cơ hội và thách thức đối với hoạt động thương mại của việt nam với nhật bản sau khi hiệp định vjepa chính thực thực thi
Báo cáo khoa học: " Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding" ppt
... utilized in the retrieval of TM proteins to the ER and that binding of the SP to this motif masks localization to this organelle, thereby facilitating the mobilization of the glycoprotein complex ... unclear at this juncture whether GPCTMICFLAG and GPCTM-FLAG proteins are specifically but inefficiently cleaved by SKI-1/S1P protease as a result of normal virus protein processing or if this phenomenon ... company, such as founder's and incentive stock options, as it pertains to the execution of this work This publication may, in part, result in the seeking of additional funding, public or private,...
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Lecture 3 DNA RNA and protein synthesis great
... All living things are made ofWater (an inorganic compound) Other inorganic compounds (mostly salts) Organic ... Lipids(fats) Proteins* Nucleic Acids* *These are what we’ll talk about today When we look at a living thing, What we see is mostly PROTEIN So, how does an organism produce its particular protein? ... A polymer {a compound made of repeating subunits} WHAT DOES “DNA” STAND FOR? DNA’s proper name isDeoxyribonucleic acid! Consists of a ribose SUGAR with a “missing oxygen” (that’s the de-oxy...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... was unable to use this RNA as template for RNA synthesis It could be argue that this data results from a misfolding of this short RNA due to a bimolecular association However, this last hypothesis ... deleted Results with this construction displayed a slightly but significantly reduced RNA synthesis in the presence of heparin compared to that observed in the absence of this compound Our further ... Fig 6A, in the absence of heparin this RNA gave a major RNA product of the template size (282 nt) and a slower migrating product twice the size of the template This high molecular mass RNA was...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... mature peptide was the only one that had been isolated [3] In this work, we have applied the techniques of molecular biology to this important species of industrial exploitation We have isolated, ... * indicates the conserved amino acids within penaeid species ‘:’ indicates similar amino acid The gray shaded boxes indicate the amino acids conserved within the CHH family neuropeptides A B Fig ... muscle or stomach This is in agreement with previous finding that have described the eyestalk X-organ–sinus gland complex as the principal source for the CHH family of neuropeptides [1] This result...
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase pot
... mM dithiothreitol, at room temperature for h After washing with buffer A, membranes were transferred to RNA binding buffer (10 mM Tris/HCl, 100 mM NaCl, 5% glycerol, mM MgCl2, 0.2 mM dithiothreitol, ... visualized by ethidium bromide staining The expected fragments were 228 nucleotides (newly synthesized minus-strand RNA) and 373 nucleotides (synthesized plus-strand RNA) in length host factors This result ... lowed to 18 °C, and protein expression was induced for 20 h by the addition of 0.4 mM isopropyl thio-b-D-galactoside (IPTG) The cell pellet obtained from 500 mL of culture was resuspended in 30...
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cdna preparation and characterization
... units of XhoI Repeat this procedure for the cut-check sample and incubate it for hr at 37° To reduce the background of the vector, a third cleavage can be performed at a third site on the A DNA ... s are localized within dendrites and raised the notion that dendritic mRNA-targeting mechanisms in the dendrite existed Translation of a reporter-tagged m R N A construct within isolated dendrites ... single growth cone, as shown in Fig 3, provided a broad view of the potential mRNA species within this region 1LFurther analysis, such as sequencing or subcloning, can be performed to distinguish...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35 were transformed into the BL21 strain of E coli, and expression of the respective proteins was induced by isopropyl-ß-D-thiogalactopyranoside ... VP30 expression in MARV infected cells resulted in decreased levels of all other viral proteins This suggests a role for VP30 in replication or transcription The self-interaction of NP is the ... coil 1: aa 320– 348, and coiled coil 2: aa 371–400, coiled coil + 2: aa 320–400) were generated within NP by inverse PCR (Imai et al., 1991) and pT-NP as template [6] Plasmids containing the required...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
... RNA-replication reactions This mutant protein exhibited a 2-fold stimulation in RNA synthesis, only slightly lower than what is obtained with 3CDpro(3CproH40A) (Fig 2A) This result indicates that ... possibility that this protein has an additional role at a subsequent step in the viral life cycle, the encapsidation of the progeny viral RNAs To examine at which step of assembly this might occur, ... label that appear in this 80S peak following SDS treatment is not derived from the 155S peak, presumably by the dissociation of provirions into 80S empty capsids and RNA (Fig 6A) This suggested to...
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Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35 were transformed into the BL21 strain of E coli, and expression of the respective proteins was induced by isopropyl-ß-D-thiogalactopyranoside ... VP30 expression in MARV infected cells resulted in decreased levels of all other viral proteins This suggests a role for VP30 in replication or transcription The self-interaction of NP is the ... coil 1: aa 320– 348, and coiled coil 2: aa 371–400, coiled coil + 2: aa 320–400) were generated within NP by inverse PCR (Imai et al., 1991) and pT-NP as template [6] Plasmids containing the required...
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Báo cáo hóa học: "Preparation and characterization of carbon nanofluid by a plasma arc nanoparticles synthesis system" pot
... The thickness of carbon nanoparticles is much smaller than its length and width in the photographs Overall, the shape of these nanoparticles is of the shape of flakes (d-Spacing about 0.35 nm) This ... nanofluid, and cannot cover all factors Since this study is made of non-spherical carbon nanoparticles, Maxwell’s equation will show an undervalue Moreover, this study found that low concentrations ... conductivity of carbon/water nanofluid Furthermore, the carbon nanoparticles made in this study are flake shaped, in which the thickness of the nanoparticle is much smaller than the length and width respectively,...
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Báo cáo hóa học: " Intermatrix synthesis: easy technique permitting preparation of polymer-stabilized nanoparticles with desired composition and structure" ppt
... sensitivity in this case is far lower than that of sensors modified with Cu0NPs after identical treatment Conclusions The main conclusion, which can be derived from the results of this study, concerns ... on IMS of low-solubilitymetal-salt-NPs (i.e metal sulphide NPs) and nanocomposites on their base This communication reports the use of IMS of CuS and PbS INPs along with characterization of the ... bi-metallic core-shell, tri-metallic core-sandwich, etc) The results presented in Figure confirm this hypothesis showing TEM images and EDS spectra of bi-metallic core-shell Pt@Cu (Figure 1a, b)...
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Báo cáo khoa học: "Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture" pot
... grow within 1-2 weeks Among the media tested, the highest multiplication rate was obtained on low salt media (BTM, WPM) supplemented with a low concentration of cytokinin (BAP 0.2-0.6 mg·l Within ... in our experiments with vegetative propagation of Q robur and Q petraea by cuttings and by tisIn this sue culture are presented and discussed MATERIALS AND METHODS Propagation by cuttings Leafy ... containing a low concentration of auxin (IBA or NAA 0.2-1.0 mg·l stimulated root induc) -1 tion Within 2-3 weeks, 68-92% of microshoots of juvenile origin (depending upon the clone) produced roots...
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Báo cáo sinh học: "Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer" pps
... Roughly one-third of these patients progress to incurable metastatic disease, for which there are few treatment options The chemotherapy currently used has limited efficacy [3,4] This lack of ... HLA-A2-restricted peptides [13,14] For other populations, this approach might not be an option because of the high genetic diversity observed This is the case for the Brazilian population, which consists ... mismatch among patients and leukapheresis donors This difference can provoke immune responses against donor DCs instead of the tumor proteins that they carry This problem could be solved by using patient...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... have influenced this book in less direct but more fundamental ways: Steve Boyd, Janice Fiamengo, Scott McFarlane and Tarik Kafala have all insisted on the larger contexts within which this sort of ... in this period It is not only by understanding more about each of them, but also by trying to think through their points of interconnection, that we can better recognize our own implication within ... plans for a panopticon – which reversed this dynamic by emphasizing the visibility of the subjects rather than the rulers Whereas Foucault’s sense of this historical shift is pessimistic (modern...
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Cambridge.University.Press.War.Land.on.the.Eastern.Front.Culture.National.Identity.and.German.Occupation.in.World.War.I.May.2000.pdf
... place Since this study deals above all with German perceptions of the occupied East, which military authorities claimed as a ‘‘New Land’’ for German administration, this study uses German names given ... underlined this ethnic complexity, especially the amazing case of the ‘‘three Smiths’’ in the Lithuanian town of Mariampol Besides a family by the German name of Schmidt, there were also in this multilingual ... historical circumstance and (to them this seemed most obscene) on personal choice and commitment Whatever feelings came to them at the sight of this variety, one thing was clear to Germans They could...
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IELTS Writing Preparation
... Someland What you think? What is your opinion of this sample answer? How well does it meet the requirements of the guidelines? Read the next page for a teacher's comments on this answer © 1999 ... Describing the difference between two levels This year unemployment has increased by 20,000 cases (the difference between this year and last year is 20,000 cases) This year there has been an increase ... ended their schooling at this point This is in stark contrast with 1945 when only 30% of girls completed primary school, 35% had no schooling at all and 35% only completed the third grade In conclusion,...
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Preparation And Practice
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