control of adult stem cell function in bioengineered artificial niches matthias p lutolf and helen m blau
Characterisation of lung dendritic cell function in a mouse model of influenza
... Figure 5.9.1 Intracellular staining of TAP1 and TAP2 in DCs from lung and pMLN 161 Figure 5.9.1 Intracellular staining of TAP1 and TAP2 in DCs from lung and pMLN (continued) ... CD8 T -cell with TCR specific for OVA257-264/Kb PBS Phosphate buffered saline PE Phycoerythrin PerCP Peridinin-chlorophyll protein PCR Polymerase Chain Reaction PFA Paraformaldehye P. I Post Infection ... results in the induction of inflammatory responses against inhaled non-immunogenic proteins In vivo imaging of CD4 T -cell and lung DC interactions in the lung draining lymph node by two-photon...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2
... containing 20% glycerol, 1mM mercaptoethanol, 1mM EDTA, 1mM sodium orthovanadate (SOV), 1mM PMSF, 1mM Aprotinin, 1mM Leupeptin and 1mM Pepstatin A The buffer was stored at 4℃ and the protease inhibitors ... inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using Substrate preparation: 1mM D-erythro-sphingosine was prepared by mixing 20μl sphingosine (50mM) with ... pro-monocyte lymphoma cell line The cells were cultured in RPMI 1640 supplemented with 10% FBS (GIBCO, Invitrogen Singapore), 2mM glutamine, 10U/ml penicillin and 10mg/ml streptomycin at 37C, 5% carbon...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3
... isobutyl-methylxanthine, 1 M dexamethasone, 10 M insulin, 200 M indomethacin, 1% Penicillin and Streptomycin DMEM+10% FBS 0.01 M 1,25-dihydroxyvitamin D3, 50 M +1% Penicillin and ascorbate-2-phosphate, 10mM ßStreptomycin ... Expression of SPHK1 and SPHK2 in Human BM- and ADMSCs Human BM-MSCs and AD-MSCs were tested on their mRNA expression of SPHK1 and SPHK2 using real time PCR, and the results are summarized in ... more fatty acid binding protein gene expression than 1 M of CP6 did; and 1 M of CP6 treated adipogenic-induced cells expressed more fatty acid binding protein mRNA than 0.5 M of CP6 Interestingly,...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4
... are shown in Figure 4.5 S 1P function on human MSCs proliferation * cell growth (fold) ** BM-MSCs AD-MSCs ** * ** DMEM DMEM+10%FBS DMEM+100nM S 1P DMEM+500nM S 1P DMEM+1uM S 1P DMEM+5uM S 1P culture ... cultured in S 1P- supplemented media were tested for their stemness maintenance In particular, cells cultured from serum-supplemented or S 1P- supplemented media were compared for their morphologies, ... shown in Figure 4.7 4.7A S 1P+ PDGF-AB function in human MSCs proliferation (4 days) cells growth (fold) * ** BM-MSCs * ** * * ** ** AD-MSCs DMEM DMEM+10% FBS DMEM+1uM S 1P DMEM+20ng/ml PDGF-AB DMEM+1uM...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5
... potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... Work procedure of extracting these cells is much less painful, compared with extracting MSCs from BM This study investigated the function of S 1P, S 1P+ 10%FBS, S 1P+ 1%FBS and PDGF-AB in human AD-MSCs ... serum, can be an important element in the development of better defined serum-free or serum-reduced strategies to promote human -adult BM- and AD- MSCs proliferation S 1P is much cheaper than most...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6
... “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” Biochemistry, ... of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment ... “Charaterization of N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18-25 Kim, Hyo-Lim,...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation
... S1PRs, S1PR1, S1PR2 and S1PR3 are most widely expressed, while S1PR4 is mainly found in hematopoietic system, and S1PR5 is predominantly expressed in brain and spleen (MacLennan et al., 1994; McGiffert ... effect of SPHK and its product S 1P on immune cells such as monocytes, neutrophils, mast cells and eosinophils, suggest that SPHK and its product S 1P may play key roles in inflammation In humans, ... surface markers, maintained by S 1P+ PDGF Thus, it indicates the involvement of endogenous SPHK and/ or S 1P in stem cell proliferation and multipotency maintenance It is well known that in stem cells,...
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báo cáo hóa học:" Chemomodulation of human dendritic cell function by antineoplastic agents in low noncytotoxic concentrations" pdf
... antitumor immunity in tumor-bearing hosts In addition, CD40 ligation protects human and murine DCs from tumor-induced apoptosis by inducing expression of anti-apoptotic proteins from the Bcl-2 family ... (Cytoxan) and mitomycin C (Mutamycin); the topoisomerase inhibitor doxorubicin (Adriamycin); the platinum agents cisplatin (Platinol) and carboplatin (Paraplatin); the hormonal agents flutamide (Drogenil, ... cell carcinoma (UPCI, Pittsburgh, PA, USA), and HCT-116 and HT-29 colon adenocarcinomas (ATCC) Cells were cultured in RPMI 1640 medium supplemented with 10% FBS, mM Lglutamine, mM sodium pyruvate,...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
... explained by the presence of 46 SNPs upstream of the minimal promoter of the IL-2 gene that can alter the transcriptional activity of this gene compared to NOD mice [22] Moreover, polymorphisms ... MOP67211 and CIHR MOP84041 grant from the New Emerging Team in Clinical Autoimmunity: Immune Regulation and Biomarker Development in Pediatric and Adult Onset Autoimmune Diseases C.A .P holds a ... choice of immunosuppressive drug therapy Cell Cycle 2008, 7:458-462 Monti P, Scirpoli M, Maffi P, Piemonti L, Secchi A, Bonifacio E, Roncarolo MG, Battaglia M: Rapamycin monotherapy in patients...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 1) ppt
... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... specific cell types, minimizing the risk of tumor formation or proliferation of inappropriate cell types, ensuring the viability and function of the engrafted cells, overcoming immune rejection ... as heart and brain Substantial efforts have therefore been devoted to obtaining more pluripotent stem cell populations, such as bone marrow mesenchymal stem cells (MSCs) or adipose stem cells,...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 2) pps
... infarction, functional improvements have been achieved after transplantation of a variety of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose ... stem cells Bone marrow stem cells in particular have been examined in clinical trials of human ischemic heart disease These have largely been small, nonrandomized studies that typically combine ... drugs may be injected to stimulate endogenous stem cell populations Disease-Specific Stem Cell Approaches Ischemic Heart Disease and Cardiomyocyte Regeneration Because of the high prevalence of...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 3) pot
... oligodendroglia, and astrocytes These neural stem cells have been transplanted into the rodent brain with formation of appropriate cell types and no tumor formation Multipotent stem cells present in the adult ... Two clinical trials of fetal nigral transplantation failed to meet their primary endpoint and were complicated by the development of dyskinesia Transplantation of stem cell derived dopamine-producing ... and a clinical trial of fetal neural stem cells in Batten disease is commencing Transdifferentiation of bone marrow and adipose stem cells into neural stem cells, and vice versa, has been reported,...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 4) pot
... 2007 [PMID: 17671448] National Institutes of Health: Stem cell information page URL: http://stemcells.nih.gov/index.asp Puceat M, Ballis A: Embryonic stem cells: From bench to bedside Clin Pharmacol ... of critically ill patients Health care providers and experts with backgrounds in ethics, law, and sociology must help guard against the premature or inappropriate application stem cell therapies, ... Mimeault M et al: Stem cells: A revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications in regenerative medicine and cancer therapies Clin Pharmacol Ther 82(3):252,...
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Báo cáo sinh học: "Growth control of the eukaryote cell: a systems biology study in yeas" ppt
... Gre 2p, Tsl 1p, Tps 1p, Eno 1p Pan 5p, Mdh 3p, Npt 1p Pol3 0p, Hsp10 4p, Rvs16 1p Lap 3p, Akr 1p, Ycf 1p, Fet 3p Skp 1p, Pre 3p, Rpn 3p, Kar 2p Sec1 7p, Tpm 1p, Vtc 2p, Vtc 3p Pet 9p, Sar 1p, Fet 3p, Hxt 3p Ncp 1p, Erg 1p, ... proteins (for example, Kap9 5p, Kap10 4p, Kap12 3p, Crm 1p, Mtr1 0p, Nup 2p) , mitochondrial proteins (for example, Mpm 1p, Mis 1p, Mdj 1p) , vacuolar proteins and proteins involved in vacuolar protein sorting ... (7) Proteins (examples) p- value (GO studies) Ser 3p, Cpa 1p, Lia 1p Rpl1 0p Gln 1p, Leu 1p, Lys 4p Rpl6ap, Mes 1p, Sui 3p, Fun1 2p Mdh 2p, Rpl 3p, Mes 1p, Ths 1p Mes 1p, Cdc6 0p, Ded8 1p Nug 1p, Rpl3 0p, Nop5 8p, ...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... calf serum, mmol/l L-glutamine, mmol/l pyruvate, 50 μmol/l mercaptoethanol, 100 U/ml penicillin, and 100 μg/ml streptomycin, and then incubated in plastic cell cultures plates for h at 37°C in a ... the development of airway inflammation and Th2 responses and suppressed the proliferation of peripheral CD4 +and CD8+ T cells and the expression of Foxp3+ Treg in spleen, which could be prevented ... thickness of small airway walls and the number of infiltrated eosinophils around the airway increased, eosinophils appeared within the lumen of the airway, and goblet cell hyperplasia and hypertrophy...
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Asymmetric cell division in the regulation of neural stem cell self renewel in drosophila melanogaster
... Molar MgCl2 mins Mira ml mM MOPs MTOC Mts Mud NB Magnesium chloride minutes Miranda millilitre millimolar 4-Morpholinepropanesulphonic acid microtubule organizing center Microtubule star Mushroom ... N-terminal OD PAGE PAN Par PBS PCM PCR PH3 Pins PON PP2A Pp4 Pros RNA RNAi RT RT-PCR S SDS secs Amino (NH2) terminal optical density Polyacrylamide gel electrophoresis Posterior Asense-Negative partitioning ... interaction Par-6 is a small protein that contains a PDZ domain, a PB1 domain and an atypical CRIB domain Par6 binds aPKC through the PB1 domain, and binds Rho GTPase Cdc42 through the CRIB domain Since...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... expressed in a particular cell type may play a critical role in the regulation of cellular 41 processes specific to that cell The in depth transcriptome profiling by Wei et al (2005) using MPSS also ... in further examining the functional role of Lefty2 in ES cells For this purpose, a loss of function approach using RNAi was employed Ablation of Lefty2 induced differentiation in both mouse and ... experiment to dispel this argument (Figure 23) SB431542 is a specific competitive ATP binding site kinase inhibitor, and it inhibits signaling by inhibiting the phosphorylation of SMAD2 and SMAD3 by ALK4/5/7...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... implicated in embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part ... embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have both previously ... cells into meso/mesendodermal lineages while repression of signaling with the specific chemical inhibitor SB431542 and Lefty protein supplementation induces trophectodermal differentiation This...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... were maintained on matrigelcoated dishes in conditioned medium containing knockout DMEM/10% serum replacement (Invitrogen), 0.1 mM MEM nonessential amino acids (Invitrogen), mM lglutamine (Invitrogen), ... serum replacement (Invitrogen,Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), 0.1 mM β-mercaptoethanol (Invitrogen), and penicillin– streptomycin ... Briefly, the MEF conditioned medium was prepared as follow: 2X106 mitomycin treated MEF cells were plated in 100mm dishes and cultured in 10ml TS medium containing RPMI 1640/20% FBS, 0.1 mM MEM nonessential...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... from monkey embryonic stem cells function in a Parkinson primate model J Clin Invest 115, 102-109 Takahashi K, Yamanaka S (2006), Induction of pluripotent stem cells from mouse embryonic and adult ... The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells Cell 113, 631642 Moffat, J., and Sabatini, D M (2006) Building mammalian signalling pathways with ... Formation of germ-line chimeras from embryonic stem cells maintained with recombinant leukemia inhibitory factor Exp Cell Res 190, 209-211 Pera, M F., Andrade, J., Houssami, S., Reubinoff, B., Trounson,...
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