... Parvaiz Ahmad, and Nicole Geissler Prospects of Halophytes in Understanding and Managing Abiotic Stress Tolerance Vinayak H Lokhande and Penna Suprasanna UV-B Radiation, Its Effects and Defense ... soils, withstand high soil salinity and saline water irrigation, phytoremediation and wetland restoration It will be invaluable to develop these strategies to ensure sustainability, andfuture efforts ... and uptake of ions, enzymatic and nonenzymatic antioxidant response, maintenance of redox and energetic status, salt inclusion/excretion and genetic control (Flowers and Colmer 2008) Understanding...
... eagle medium (Gibco, Grand Island, NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained ... the expression of b1- and b2-ARs was determined at both the mRNA and protein level in SGC-7901 and BGC-823 cells by RT-PCR and western blot Our results showed that b1- and b2-adrenergic receptors ... the data and plotting of the figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 and BGC-823...
... analysis and helped to draft the manuscript All authors read and approved the final manuscript References Methods Cell lines and chemical inhibitors T98G and U373-MG human glioblastoma cells and CHOK1 ... alone, and one for the drug-treated case; and δx – where "x" is any of the parameters above – is the variation on x between the control and case under study General least square fitting was used and ... cytotoxic and radiosensitising effects, and to ascertain whether the two are interdependent The aim of this project was to devise a general approach that could be applied to complex survival curves and...
... UV-B-induced stimulation of MBPK and CDPK activities and the UV-Binduced accumulation of Tdc and Str mRNAs, and catharanthine Because EGTA and verapamil are unlikely to enter cells, and verapamil blocks ... IIIS (a) and MBP (b) under standard conditions as described in materials and methods A second set of cells was similarly treated and the total RNA was isolated at the indicated times and analyzed ... for 10 with 0.1 and mM suramin concentrations and sub- sequently irradiated with UV-B for min, the UV-Binduced MBPK and CDPK activities, accumulation of Tdc and Str transcripts and catharanthine...
... system at a superfund site Photocatalytic Purification and Treatment of Water and Air, Edited by D F Otlis and H Al-Ekabi, pp 547-556 Raquel F P N and Welson F J (1996) TiO2-fixed-bed reactor for water ... on the degradation rate of TCE, and the byproducts from the TCE degradation were examined MATERIALS AND METHODS TCE (99+%) was obtained from Aldrich Chemical Co, and the TiO2 used was Degussa P-25, ... which was mostly anatase and had a BET surface area of 50-m2/g and an average particle diameter of 30 nm The extraction solvents used in this study were n-hexane (Burdick and Jackson, GC quality)...
... excitation and emission of nm and quartz cells of 1-cm path length; the excitation and emission were recorded at wavelengths of 496 and 516 nm, respectively The A laidlawii B cells were grown and cell ... and sound velocimetry that GS binding to PtdCho bilayers decreases the temperature and cooperativity of their gel/liquidcrystalline phase transition and increases the volume compressibility and ... headgroups and above the fatty acyl chains, and that GS penetrates more deeply into anionic and more fluid phospholipid bilayers [14] Finally, Ó FEBS 2002 using solid-state 19F-NMR spectroscopy and a...
... mutual effects of pH and NaCl on the bimolecular association rate constant of O2 rebinding and the 6110 quantum yield of BR for the a and b subunits within the liganded dimer and tetramer of hemoglobin ... Kt, for the last ligand binding step: Kt ¼ K1 Á K2 K1 þ K2 ð5Þ Here, K1 and K2 correspond to the affinity of O2 binding to the a and b subunits within the triliganded (monoliganded) tetramer (dimer), ... the triliganded HbA (Table 1, ), but only one in every 20 ligands leaves the a subunits (Table 1, ), and in every six ligands leaves the b subunits (Table 1, ) Using Eqns (3) and (4),...
... in b-cell dysfunction and apoptosis in rodent models of diabetes [37–40], and changes in mitochondrial function, including increased ROS and UCP2 expression, lower ATP and a decreased ATP ⁄ ADP ... two bands in cells incubated with recombinant PHB, the top band corresponding to His-tagged PHB (Fig 5A) These data indicate that exogenous PHB enters the cells PHB is expressed in b-cells and ... described in Materials and methods The results are expressed as the mean ± SEM percentage of G2 for (A) and (B); as the mean ± SEM arbitrary units relative to actin for (C); and as the mean ± SEM...
... IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- (black bars) mixed glial cultures Cultures were treated with LPS or LPS+IFNg for h and then ... C/EBPb+/+ (white bars) and C/EBPb-/(black bars) show similar total cell numbers and microglial density in control conditions and after 16 h of LPS or LPS+IFNg A C/EBPb+/+ and C/ EBPb-/- total cell ... comparison between LPS and LPS+IFNg effects on C/EBPb and on pro-inflammatory markers in glial cells We have observed that both LPS and LPS +IFNg induce similar increases in C/EBPb mRNA and protein levels...
... osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand-receptor system that directly regulates osteoclast differentiation and bone resorption, and both are produced and secreted ... Orthopaedic Surgery and Research 2010, 5:83 http://www.josr-online.com/content/5/1/83 expressed in brain tissue, adrenal and pituitary glands, the exocrine pancreas, peripheral tissue and on osteoblasts ... cells [13,14] On the one hand, RANKL binds to RANK, which is expressed on osteoclast progenitors, and leads to osteoclast activation On the other hand, OPG binds to RANKL and thereby inhibits osteoclast...
... osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand-receptor system that directly regulates osteoclast differentiation and bone resorption, and both are produced and secreted ... Orthopaedic Surgery and Research 2010, 5:83 http://www.josr-online.com/content/5/1/83 expressed in brain tissue, adrenal and pituitary glands, the exocrine pancreas, peripheral tissue and on osteoblasts ... cells [13,14] On the one hand, RANKL binds to RANK, which is expressed on osteoclast progenitors, and leads to osteoclast activation On the other hand, OPG binds to RANKL and thereby inhibits osteoclast...
... {kobs of control - kobs of fullerene}/kobs of control) of PVP/C60 and C60O were 50% and 68% in 10 lM for antioxidant, and 73% and 81% in 30 lM, respectively Here, it is more convenient to define ... (for C60), 1.33 (for C60O), and 1.93 (for C60On), represent the efficiency of the antioxidants and thus C60O and C60On react with the LOO• radical approximately 1.7 and 2.4 times faster than C60 ... ethanol, pure water (5 mL) was added and mixed again for 30 The mixture was centrifuged and the obtained solution was filtered through a membrane filter (0.45 and 0.1 lm) to give a clear purple solution...
... the fetal period, as was also reported by Konermann [11] and Rugh and Wohlfromm [28], and supports the earlier conclusions of Russell [29] and others [25, 33, 37] that the period of organogenesis ... damage, agree with the conclusions of Russell [30, 31] and Uma Devi and Baskar [33] Maximum lethality was found after exposure on day 2.5 p.c A similar observation was made by Rugh and Wohlfromm [28], ... mice and some seasonal effects in the perinatal period Radiat Res 1965, 25, 611-625 Kimmel, C A and Trammell, C A rapid procedure for routine double staining of cartilage and bone in fetal and...
... skull and extremities The threshold doses of radiation that induced cleft palate and dilatation of cerebral ventricle, and abnormal extremities were between 1.0 and 2.0 Gy, and between 0.5 and ... mice and some seasonal effects in the perinatal period Radiat Res 1965, 25, 611-625 Kimmel, C A and Trammell, C A rapid procedure for routine double staining of cartilage and bone in fetal and ... 70, 45-52 Uma Devi, P and Hande, M P Effect of low dose 70 kVp X-rays on the intrauterine development of mice Experimentia 1990, 46, 511-513 Uma Devi, P., Baskar, R and Hande, M P Effect of exposure...
... cancer and overcoming resistance to standard genotoxic chemo- and radiotherapy Importantly, Akt is embedded into a highly complex network of upstream regulators and downstream effector proteins and ... performed conception and design of the study and substantially contributed to interpretation of data, drafting and critical revision of the manuscript and final approval All authors read and approved ... Western blotting analyzing caspase-3 and PARP cleavage (C, D) Cleavage of caspase-3 (PC3 and LNCaP cells) and PARP (LNCaP cells) was more effective when ErPC3 and ionizing radiation were combined...
... the associations between tTATI and s-TATI concentrations, before and after RT, and clinicopathological parameters, including s-CEA and s-creatinine Classification and regression tree (CRT) analysis ... shown) Association between TATI in tissue and serum and clinicopathological characteristics Figure TATI expression in tumour tissue and normal mucosa before and after radiotherapy Box plots showing ... Angenete E, Oresland T, Falk P, Breimer M, Hultborn R, Ivarsson ML: Preoperative radiotherapy and extracellular matrix remodeling in rectal mucosa and tumour matrix metalloproteinases and plasminogen...
... BM and migrate via peripheral blood (PB) to secondary lymphoid tissue such as the spleen and lymph nodes Here they change/switch the expression pattern of Ig from IgD and IgM to IgG, IgA and ... lymphoma and after autologous stem cell transplantation [29,34] Leandro and colleagues described a depletion of mature BM B cells three months after rituximab treatment, while pro- and pre-B ... (Three Star Inc., Ashland, OR, USA) Collection of blood and BM samples Heparinized blood and BM aspirates of a volume of 10 ml each were obtained at baseline (n = 37) Blood and BM sampling was...
... NF-κB1 (p50 and p105), NF-κB2 (p52 and p100), c-Rel, and RelB These transcription factors are sequestered in the cytoplasm by inhibitory (I) κBs, which prevent NF-κB activation, and inhibit nuclear ... Upland, CA, USA) The relative density of the DNA-protein binding bands was scanned by densitometry using MyImage (SLB, Seoul, Korea), and quantified by Labworks 4.0 software (UVP Inc, Upland, ... and p-IκB (1:500 dilution, Santa Cruz Biotechnology Inc Santa Cruz, CA, USA), rabbit polyclonal for p65 and IκB (1:500 dilution, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and iNOS and...
... blood and RA synovial B cells express CCR5, CCR6, CCR7, CXCR3, CXCR4, and CXCR5 The ligand chemokines, CCL3, CCL4 and CCL5 for CCR5, CCL20 for CCR6, CCL19 and CCL21 for CCR7, CXCL9, CXCL10 and ... interpretation, and manuscript preparation KT and YK participated in the data analysis and interpretation, and assisted in manuscript preparation TM, HK, AN, PEL, and NM assisted in data interpretation and ... also them Therefore, we selected four chemokines, CCL20, a ligand for CCR6, CCL19 and CCL21, ligands for CCR7, and CXCL12, a ligand for CXCR4 First, we analyzed the effects the chemokines on...