clinical and laboratory aspects of preimplantation genetic diagnosis and derivation of affected human embryonic stem cell lines
báo cáo khoa học: "A nano-view of West Nile virus-induced cellular changes during infection" pps
... Arrows show greater degree of thickening and clumping of actin filaments in close proximity to the cell periphery of the infected cell at 16 h p.i (c) AFM 3D height scan of 15.3 µm × 15.3 µm size ... from Professor E G Westaway, Sir Albert Sakzewski Virus Research Laboratory, Queensland, Australia), was propagated in Vero cells throughout this study Flavivirus infection of cells The Vero cells ... forming near the cell edge and the nascent filopodia (arrowheads) (d) AFM height scan of mock-infected Vero cells at 24 h p.i Scan size is 30 µm × 30 µm This control cell shows a lack of cytoskeleton...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... helix of the CTD which pack against the N-terminal end of helix of the NTD of a neighboring subunit in the hexamer Additional contacts involve helix 11 of the CTD and the C-terminal end of helix of ... oligomerization affinity of CA and the stability balance of the HIV capsid may be a result, in part, of the preservation of intersubunit electrostatic repulsions Conformational rearrangements of CA and HIV-1 ... of both hexamers and pentamers using essentially the same structural elements of CA [40]; (e) the different interactions of CA with several ligands and their effects on capsid assembly [3]; and...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being ... deletion affected the hinge between stem loops SL-E1 and SL-AII Notably, the apical loop and the upper part of the stem of SL-E1 has a primary sequence homology with the SL-II stem loop of the ... involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA Results Effect of mutations in the 3¢- or the 5¢-end of...
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Báo cáo hóa học: " Therapeutic immunisation of rabbits with cottontail rabbit papillomavirus (CRPV) virus-like particles (VLP) induces regression of established papillomas" docx
... better understanding of the molecular mechanism that regulate normal cell growth, steps involved in cancerous cell changes [7] and have examined the efficacy of several delivery systems [5,8-10] ... demonstrated that the expression of PV L1 genes in a number of cell systems results in the assembly of virus-like particles (VLPs), which elicit high titers of virus-neutralizing serum antibodies ... lesions of the cervix, vulva, and vagina and effective against genital warts [13-15] Owing to the promising human clinical trials, this prophylactic quadrivalent HPV (types 6, 11, 16 and 18)...
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Báo cáo hóa học: " Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection" potx
... 0.5 μl of reverse-transcription product, 0.1 mM of each primer and μl of QuantumRNA™ 18S internal standard (Ambion, Austin, TX) at a primer:competimer ratio of 4:6 for CYP 3A2 and 2C11 and 3:7 ... dilution of samples and infection of HeLa cells as described [53] Administration of Recombinant Adenovirus All procedures were approved by the Institutional Animal Care and Use Committee of The ... last decade, extensive use of adenovirus serotype as a vector for gene therapy and vaccine development has increased understanding of the biology and the genetic features of the virus This information...
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Báo cáo hóa học: " Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses" pot
... in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and non -human primates Blood 2002, 100:823-832 Sandrin V, Cosset FL: Intracellular versus cell surface ... Biochemical and functional analysis of Flu-VLPs Biochemical and functional analysis of Flu-VLPs A Incorporation of HA, NA and M2 proteins from H7N1 (A/Chicken/ FPV/Rostock/1934) or H5N1 (A/Thailand/KAN-1/04) ... factor of the viral supernatant and "%inf" is the percentage of GFP-positive cells as determined by FACS analysis using dilutions of the viral supernatant that transduced between 0.1% and 5% of GFP-positive...
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Báo cáo khoa học: " Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes" ppsx
... cellular cytotoxicity in human neutrophils Immunol Cell Biol 1995, 73, 234-238 26 Shoda LKM, Rice-Ficht AC, Zhu D, McKnown RD and Brown WC Bovine T cell responses to recombinant thioredoxin of ... present study is the first to investigate levels of IFN-γ, IL-6 and IL-8 during infection with F gigantica Results show that the T cell response of cattle and buffaloes infected with F gigantica in ... response commenced early and persisted throughout the 16-week observation period IL-6 is one of the cytokines produced by Th2 cells [1,7] and it participates in the polarization of the immune response...
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Báo cáo y học: "Altered expression of inflammatory cytokines in primary osteoarthritis by human T lymphotropic virus type I retrovirus infection: a cross-sectional study" pps
... proliferation; however, several clinical issues remain unresolved There is still no established criterion for the diagnosis of HAAP because of the lack of specific symptoms and/ or laboratory markers Moreover, ... degree of proliferation of synovial lining cells was assessed in at least five points in an area of more than 1.5 cm × 1.5 cm as follows: -, or cells thick; +/-, or cells thick; ++, 5–9 cells ... onset, and the changes in disease status over a few years are often small and difficult to quantify Concentrations of chondrocalcin (a marker of articular cartilage damage) and DPD (a marker of subchondral...
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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx
... Effects of VLPs IL-10 shRNA on morphology and viability of RAW 264.7 cells (A) Two preparations of IL-10 shRNA (IL-10i-1 and IL-10i2) were packaged into JCV VLPs by osmotic shock The presence of IL-10 ... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... μg of JCV VLPs with IL-10 shRNA, or irrelevant shRNA, respectively After 6, 12 and 24 h of treatments, serum samples were collected and tested for IL-10 and TNF-α levels by real-time PCR and...
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báo cáo khoa học: "Stability and assembly in vitro of bacteriophage PP7 virus-like particles" pptx
... junction of the of the 5'- and 3' is shown at ThePP7 bottom, and the sequences2PP7itduplication ends of The sequence at the junction of the 2PP7 duplication is shown at bottom, and the sequences of ... the structure of the icosahedron Partial reduction of the PP7 VLP results in the appearance of monomers, and multiples of monomers up to the size of hexamers However, VLPs made of single-chain ... Figure of DTT reduction of PP7 and 2PP7 VLPs with varying concentrations SDS polyacrylamide gel electrophoresis of the products of SDS polyacrylamide gel electrophoresis of the products of reduction...
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Thermal Stability of RNA Phage Virus-Like Particles Displaying Foreign Peptides potx
... transcription terminator, and the polylinker of pET3d and the kanamycin resistance determinant of and origin of replication of pET9 Note that detailed information about pET3d and pET9, as well as ... a function of temperature in the presence and absence of a reducing agent (DTT) (B) Disassembly and aggregation of the Flag-PP7 single-chain dimer VLP with and without DTT Caldeira and Peabody ... insertion of ten NNS triplets between codons for amino acids 13 and 16 in the MS2 AB loop The denaturation profile of the random 10-mer library (~109 individual Caldeira and Peabody Journal of Nanobiotechnology...
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Báo cáo y học: "Kinetics and isotype profile of antibody responses in rhesus macaques induced following vaccination with HPV 6, 11, 16 and 18 L1-virus-like particles formulated with or without Merck aluminum adjuvant" ppt
... to cancer HPV type 16 and 18 infection cause 70% of cancer cases and 25% of low grade cervical dysplasia [8] HPV types and 11, on the other hand, cause approximately 95% of genital warts (condylomata ... warts) and 25% of low grade cervical dysplasia (CIN1) [9,10] Thus, a vaccine targeting HPV 6, 11, 16 and 18 will target the majority of HPV-related clinical disease Recently, we reported a proof -of- concept ... IL-4-producing T-helper cells, high levels of neutralizing Abs and low levels of IFN-γ secreting CD8+ or CD4+ Page of 11 (page number not for citation purposes) Journal of Immune Based Therapies and Vaccines...
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Báo cáo y học: " Human herpes virus 8 replication during disseminated tuberculosis in a man with human immunodeficiency virus: a case report" pdf
... copies/10E5 cells [4] HHV-8 DNA detection predicts the clinical evolution and the response to treatment of both KS and MCD, but its Page of (page number not for citation purposes) Journal of Medical ... was noted Discussion The clinical presentation of fever, weight loss, generalized lymphadenopathy and enlargement of the liver and spleen in our patient was suggestive of tuberculosis However, ... feature, and intense interfollicular plasma cell hyperplasia Immunohistochemistry using antibodies against latent nuclear antigen of HHV-8 allows the detection of B cells infected with HHV-8 These cells...
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Báo cáo y học: "Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever" ppt
... http://www.virologyj.com/content/7/1/279 Page 10 of 19 Figure Binding profile of human serum IgM and IgG, and NP-specific mAbs on LASV VLP and recombinant nucleoprotein Human sera collected from household contacts of patient G676, ... combination of rational design and proven genetic elements have resulted in high yields of LASV VLP These vectors afford the possibility of developing a VLP-based vaccine candidate in mammalian cell systems ... micrographs of LASV VLP budding from the surface of HEK-293T/17 cells expressing LASV Z alone or in combination with GPC and NP genes, and high magnification of LASV pseudoparticles Cells expressing...
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Báo cáo y học: " Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli" pptx
... The reaction of Cap-tag and nCap may with anti-PCV2 pig sera and was observed by Western blotting (Fig 3) Cleavage of His-Smt3 tag and assembly of Cap protein VLPs After the cleavage of the His-Smt3 ... Expression and purification of the Wrst nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding ... containing BsaI on both positive and negative strands and a BamHI restriction site were added to the 3′ end of smt3 gene After double digestion with restriction enzymes NcoI and BamHI, the amplified...
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Báo cáo khoa học: " The inhibition of assembly of HIV-1 virus-like particles by 3-O-(3'''',3''''-dimethylsuccinyl) betulinic acid (DSB) is counteracted by Vif and requires its Zinc-binding domain" pdf
... indicated on top of panels (a) and (b), and the x-axis of panel (c) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting ... indicated on top of panels (a) and (b), and on the x-axis of panel (c) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting, ... indicated on top of panels (i) and (ii), and on the x-axis of panel (C) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting,...
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Báo cáo y học: " Kinetics of hepatitis C virus RNA load during pegylated interferon alpha-2a and ribavirin treatment in naïve genotype 1 patients" potx
... to week 2, 4, 8, and 12 of treatment, week point time tend to be better than other time points for predicting SVR, with a PPV of 91% and a NPV of 89% and a very good sensitivity and specificity ... mechanisms of action of interferon as well as the consequences of varying dosages of interferon [14] The high turnover rates of HCV explain the rapid generation of viral diversity and the opportunity ... after the end of the treatment (0 = no response; = response) cycle of these viruses and their response to therapy [6] Studies of the kinetics of hepatitis C virus (HCV) after initiation of IFN monotherapy...
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Báo cáo sinh học: "The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression" docx
... follows: HepG2 Cells Detection of ribozyme RB15 RNA in cells by one-step RT-PCR A human hepatoma cell line (HepG2 cells, × 105 cells) was seeded onto each well of a six-well plate until the cells reached ... reduction of apoB protein of 62% and cholesterol and triglyceride of 42% and 51%, respectively [3] The different observations demonstrated in our studies between using an adenovirus vector and an ... conversion of single-stranded DNA to doublestranded DNA process, expressed ribozyme RB15 RNA faster and more efficiently Page of 11 (page number not for citation purposes) Genetic Vaccines and Therapy...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
... safe and effective systems for delivering antigenic and/ or adjuvant proteins/ genes to DCs, T cells or both represent valuable means of eliciting and modulating type, extent, and duration of adaptive ... stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates Blood 2002, 100:823-832 Munz C, Steinman RM, Fujii S: Dendritic cell maturation ... pcDNA3.1 plasmid and RD114/TR by the phCMVRD114/TR plasmid (kind gift of Dr François-Loic Cosset, Ecole Normale Supérieure, Lyon, France) Cell lines and primary murine cells The cells used included...
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Báo cáo khoa học: The association of viral proteins with host cell dynein components during virus infection pdf
... ASFV p54 [78] and KSTQT and KSIQI in the case of the P protein of rabies and Mokola viruses respectively [70,71] and SQTQT in the case of protein VP35 of ebolavirus [103] The presence of these sequences ... endings of peripheral nerves, such as neuromuscular junctions, to reach the central nervous system and causes lethal encephalitis in animals and humans After receptor binding, RV enters its host cells ... rabies and Mokola virus phosphoproteins as baits in yeast two-hybrid screens and retrieved DYNLL1 as an interacting partner using PC12 cells and human brain libraries [70,71] Fine mapping of the...
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