... considered to take place in the intestine following active absorption of Na? and Cl- [3, 5] In the posterior part of the intestine, including the rectum, osmolality of ingested seawater is reduced to ... essential for water absorption in the intestine to reduce osmolality of ingested seawater to isotonic or subisotonic levels, since the major driving force for intestinal water absorption is an osmotic ... during molting (stage A) observed in the present study is considered to be due mainly to increased water influx from the ambient water through the gill or gut into the body by an increase in...
... calcitoninlike immunoreactivity in the crustacean Orchestia cavimana during a molt cycle Gen Comp Endocrinol 73:80-84 Huong D.T.T., W.J Yang, A Okuno and M.N Wilder 2001 Changes in free amino ... Jesus 1987 Responses of intermolt Penaeus indicus to large fluctuations in environmental salinity Aqualcuture 64:175-184 Samocha T.M., L Hamper, C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and ... 2001 Changes in free amino acids in the hemolymph of giant freshwater prawn Macrobrachium rosenbergii exposed to varying salinities: Relationship to osmoregulatory ability Comp Biochem Physiol...
... calcitoninlike immunoreactivity in the crustacean Orchestia cavimana during a molt cycle Gen Comp Endocrinol 73:80-84 Huong D.T.T., W.J Yang, A Okuno and M.N Wilder 2001 Changes in free amino ... Jesus 1987 Responses of intermolt Penaeus indicus to large fluctuations in environmental salinity Aqualcuture 64:175-184 Samocha T.M., L Hamper, C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and ... 2001 Changes in free amino acids in the hemolymph of giant freshwater prawn Macrobrachium rosenbergii exposed to varying salinities: Relationship to osmoregulatory ability Comp Biochem Physiol...
... photo affinity labeling According to Fig 2A, the inhibitory activity in the nuclear extract showed a minimum between and h after mitosis (Fig 2A) It was of interest whether this interval coincided ... for binding to the polyanion In the (added) nuclear extract, such ligands were histones, and probably other DNAbinding proteins [2] To obtain an optimal response by the reporter polymerase to a ... dependence is shown in Fig 2A in terms of the reciprocal values, corresponding to the residual DNA polymerase activity The maximum at h after mitosis corresponded to a minimum in inhibitory activity...
... was introduced into Asia experimentally from 1978-1979, but beginning in 1996, L vannamei was introduced into Asia on a commercial scale This started in Mainland China and Taiwan Province of China ... widely cultured in countries of Asia-Pacific The introduction of white shrimp to Asia has given rise to a bloom in farming of this species in China, Thailand, Indonesia and Viet Nam in the last decade, ... L.vannamei to evaluate the effect of Mg-P-K levels containing in low salinity water in molting cycle, growth, and survival rate in order to propose potential use for white shrimp L vannamei culture in...
... of incubation at 37 C in the dark and gentle stirring TNP-ATP binding to the protein was detected as an increase of uorescence intensity in the presence of protein compared to the uorescence intensity ... intensity in its absence [23,42] Determination of eosin binding to the fusion proteins Interaction of eosin Y with GST fusion proteins was studied in similarity to Skou & Esman [43] in 20 mM Tris/HCl, ... equilibrium binding to the (L354I777)GST fusion protein that contains both the N- and P-domains Titration in fact revealed that TNP-ATP binds to a single site only, as tting ể FEBS 2004 Mapping of...
... uptake was maintained at 90% of control (P < 0.001 compared to inhibition by fructose) To show that the protection that a-crystallin provided against Na/K-ATPase inactivation was not due to the removal ... fructose by binding to a-crystallin, BSA was resealed (in the same manner and concentration as that of a-crystallin) and incubated with 50 mM fructose for h BSA (1 mgÆmL)1) was used as a control ... g, min) The ghost cells were then incubated for h at 37 °C Samples from the ghost cells and the supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase...
... in Fig 2B With an increase in pressure up to 400 MPa, kmax shifted from 334 to 340 nm with a small decrease in ¯uorescence intensity This indicates that aromatic amino-acid residues located inside ... kmax for intrinsic ¯uorescence shifted to a longer wavelength with a small decrease in ¯uorescence intensity, indicating that aromatic residues in the protein moved from hydrophobic to hydrophilic ... by up to 220 MPa, followed by a signi®cant decrease with further increases in pressure The BIPM ¯uorescence intensity of Na+-dependent ATPase increased somewhat with increasing pressure up to 100...
... [3H]ouabain binding by 16% (McKenna et al., 1993) No changes in muscle [3H]ouabain binding or NKA isoform abundance were seen in elite cyclists completing additional interval training involving x ... adaptations to exercise training in aged rats 64 2.5.6 Muscle [3H]ouabain changes following exercise training in the elderly 64 2.5.7 High-intensity exercise and training in the elderly ... isoforms in skeletal muscle single fibres was investigated in Chapter Several of the NKA isoforms are adaptable to exercise training, with various high-intensity training protocols inducing upregulation...
... et al [21] In addition to the change in pH, the ouabain concentration was increased from 0.1 to mm to completely depress the increase in the ouabain-insensitive activity due to increasing the KCl ... resultant increase in Na+ binding was regarded as Na+ occlusion In the absence of oligomycin, NaCl gradually inhibited pNPPase activity and Rb+ binding (Fig 4A,B) In the presence of oligomycin, the binding ... KCl in the presence of NaCl with or without oligomycin In the absence of oligomycin, increasing the NaCl concentration decreased pNPPase activity (Fig 2A) In the presence of oligomycin, increasing...
... MCM4 to prevent binding of MCM complex to chromatin [12] In contrast, a recent finding suggests that an intermediate level of phosphorylation of MCM4 is required for chromatin binding during interphase ... MCM proteins on licensed chromatin in the G1 phase was detached from chromatin Phosphorylation of MCM4 and other factors may be involved in stronger binding of MCM4 protein to chromatin in the ... complex to chromatin in G2 and M [9] To address this possibility, we expressed mutant MCM4 in which six amino acids (Ser or Thr) were converted to Ala or Glu to examine their binding to chromatin...
... and interacting proteins in mitotic cells in a population of asynchronous cells (data not shown) These results indicate that MLL1 and its interacting proteins dissociate from mitotic chromatins, ... further immunostaining experiments with several MLL1-interacting proteins, such as CGBP, Ash2, Rbbp5, etc., using synchronized HeLa cells Interestingly, each of these MLL-interacting proteins (CGBP, ... were localized in the less intense DAPI-stained regions in the nucleus (representing less condensed euchromatin), leaving gaps in the more intense DAPI-stained regions (representing more condensed...
... additional roles in signal-transducing function of ouabain’s interaction with Na+/K+-ATPase were attributed to tyrosine protein kinase, Src, epidermal growth factor receptor and the Ras/MAPK (mitogenactivated ... ouabainaltered Na+/K+-ATPase with neighbouring proteins (Src, growth factor receptors and Ras), i.e by protein–protein interaction In myocytes, the events from signal-transduction were interpreted ... needed to obtain Ca2+-oscillations in a reasonable fraction of the rat kidney cells [8], though oscillations were seen in a few cells exposed to 10–100 nM ouabain for h As ouabain binding to Na+/K+-ATPase...
... clear that linkage to signaling intermediates and pathways through protein–protein interactions is a common property of Na+/K+-ATPase in most, if not all, cells Using partially inhibitory concentrations ... stimulus-induced protein–protein interactions involving the Na+/K+-ATPase, the neighboring plasma membrane proteins, and the organized cytoplasmic protein assemblies An important point that is ... nonreceptor tyrosine kinases may also be activated by ouabain, the transactivation of the EGF receptor by Src is sufficient to account for the recruitments of Shc (SH-2 domain-containing protein),...
... ability to distinguish, or rather the inability to distinguish, exponential from linear growth Linear growth models The linear growth model has a long history Using interferometry on single cells, ... observed a single cell that appeared to stop growing for a few minutes This result was, and remains singular, and is reminiscent of the duplicate points in the individual human growth curves in Figure ... analysis vs integral analysis Consider the experimental problem in determining the pattern of cell growth using single-cell observations The main problem in distinguishing between linear and exponential...
... the minor capsid proteins are not the sole or even the main inducers of apoptosis in the infection process and are dispensable for cell death During infection, most of the minor capsid proteins ... cytotoxicity induced by the minor proteins (results not shown) In the late phase of SV40 infection, VP4 protein (a shorter form of VP3 that contains only the third, C-terminal hydrophobic domain) ... structural protein, is a potent inducer of cell apoptosis Our results suggest that the induction of apoptosis is related to the ability of the minor proteins to interact with intracellular membranes...
... are then incorporated into the RNAinduced silencing complex and bound to the 3¢-UTR of its target mRNA to induce either mRNA degradation or translational inhibition [2,14,15] Interestingly, unlike ... generation, and to be related to the expression of granuphilin ⁄ slp4 in insulin-producing cells [28] Moreover, miR9 and other neuronal miRs including miR125b and miR128, are involved in the Alzheimer’ ... hairpin are involved in formation of the piRNA-interacting complex (piRC) and function in the transcriptional gene silencing of retrotransposons and genetic elements in germline cells [18] Investigations...
... probably due to Mg2+ binding to the zinc binding sites (M2 and M1) It appears that in contrast to the binding of Zn2+ to the second M3 site, Mg2+ binding in the range of Mg2+ concentrations examined ... affinities differ widely An increase in the Zn2+ concentration is followed only by an increase in Vm with the remaining kinetic parameters not changing considerably According to the kinetic parameters, ... enhancing the conformational change in path C, and enabling a conformational change in path A, thereby increasing the rate of both cycles In reaction path A, binding of Mg2+ to subunit induces...
... are indicated in the panels First heatings of the sample are represented in continuous line, second heating in dashed lines, third heating in dotted lines and fourth heating in dashed-dotted lines ... streptokinase to a fibrin-targeted plasminogen activator Proc Natl Acad Sci USA 96, 8879–8883 12 Lin, L.F., Houng, A & Reed, G.L (2000) Epsilon amino caproic acid inhibits streptokinase–plasminogen ... its domains help to clarify inconsistencies existing in previous reports concerning the number of cooperative folding units in this multidomain protein We have also carried out a preliminary characterization...
... was subsequently found in germinating castor beans to convert acetyl-CoA from fat degradation into succinate and subsequently carbohydrates during conversion of fat into carbohydrate [28] The ... an ironresponsive element-binding protein (IRE-BP), which binds to mRNA of ferritin and the transferrin receptor and thus participates in regulating iron metabolism in animals [49,50] The latter ... To be able to make sense out of the data and in order to make the phylogenies tractable, we had to limit the number of proteins to be retrieved for analysis In selecting sequences, we tried to...