... all cells were scored for apoptosis, telomeraseactivity assay and respectively Telomeraseactivity assay Cellular telomeraseactivity was measured with TRAPELISA kit (Roche Diagnostics GmbH) ... distribution and apoptotic rate by DNA analysis using FCM Statistical Analysis The student s test and X2 test were used to evaluate the statistical significance of the results All analyses were performed ... [26] The highest telomeraseactivity is found in the S phase of cell cycle [27], whereas quiescent cells not possess telomeraseactivity at a detectable level Cancer cells escape senescence through...
... Expressions oftelomeraseactivityand the subunits in human tissues Four pairs of normal (N) and tumour (T) tissues from oral cancer patients were examined (A) Relative telomeraseactivityof ... processes ranging from signal transduction to RNA processing [28] Fig Telomeraseactivity after introduction of antisense oligonucleotides into various cells Results are presented as the means of ... expression oftelomerase subunits hTERT, hTR and TEP1 has been reported Expression of hTERT, and less so of hTR or TEP1, has been found to correlate with telomeraseactivity in many cancer cells...
... vitro and in xenograft-tumors in vivo [13-16] Inhibitionoftelomerase has a significant influence on cell death processes and was reported to increase apoptosis probably by loss of chromosomal ... Dockhorn-Dworniczak B: Telomeraseactivityandtelomerase subunits gene expression patterns in neuroblastoma: a molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and paraffin-embedded ... factors of cancer stem cells [33-35] These cells are defined as a small subpopulation of cancer cells, which have the ability of self-renewing andto produce heterogeneous lineages of cancer cells...
... (IVC) occlusion The slope of end systolic pressure volume points result in the end systolic pressure volume relationship (ESPVR) and describes myocardial contractility Similarly, the slope of the ... stabilization of CI values, the slopes of end systolic pressure volume relationship (ESPVR) and end diastolic pressure volume relationship (EDPVR) as parameters for myocardial contractility and elastance, ... analysis Statistical analysis was carried out using the StatView (version 5.0) for Windows software (SAS Institute, Inc, Cary, NC) for repeated assessment of hemodynamic parameters Wilcoxon test was...
... transfected with F:HEXIM1 and cellular extracts from mock and transfected cells were prepared PTEFb activity was assayed using as substrate the CTD4 peptide consisting of four consensus repeats of ... discussion of results and drafting the manuscript LL designed this study and edited the manuscript Acknowledgements We thank S Murphy for 7SK snRNA plasmid This work was supported by grants from ... co-expression of HEXIM1 Thus, it appears that while HEXIM1 represses P-TEFb activity, enforced expression of this protein does not have significant effects on TBP-mediated basal transcription...
... tests were performed using standard kits and reagents Histological assessment Fixed tissue samples were embedded in paraffin, sectioned (5 μm), and stained with hematoxylin and eosin Liver histology ... lead to increased cellular resistance against a wide variety of stressors and cytotoxic agents To this regard, it has been shown that embryonic stem cells with high levels oftelomeraseactivity ... Histology three days after surgery Histology three days after surgery Histology of pig livers three days after surgery was assessed to grade liver regeneration and hepatitis Shown are representative...
... assay, using H2O2 standards [36] This assay gives similar values to iodometric analysis (A Wright, C L Hawkins & M J Davies, unpublished results) The effects of reductants on peroxides were determined ... FEBS 2002 only one condition was compared to its corresponding control a Students pooled two-sample t-test was used In all cases signicance was assumed if P < 0.05 RESULTS Formation of peptide and ... also likely to be poor substrates for the glutathione peroxidase family, as a result of their steric bulk, and the buried position of most Tyr, His and Trp residues in proteins This hypothesis...
... glucoamylase Enzyme activity assay Two methods were used to assess MGAnt activity For rapid measurements of cell supernatants and assessment of column fractions the pNP-glucose assay was used For detailed ... valuable tool in future design and synthesis of a-glucosidase inhibitors effective against and specific to MGA These inhibitors should be promising lead candidates as oral agents for the treatment and ... mechanism of action of this class of inhibitors, 3, 5, the stereoisomers of salacinol 6, [25], and the six-membered ring analogues of salacinol (8, 9) (N S Kumar and B M Pinto, unpublished results)...
... relationships [25]: kass ẵI0 ỵ kdiss ỵ S0 =Km 3ị kdiss ẳ kvs =vz 4ị Ki ẳ kdiss =kass kẳ 5ị Kinetics for the association of HNE and Pr3 with rec-elan or rec-trappin-2 were studied as described ... maturation of elan from trappin-2 andto compare the antiproteolytic activityof both inhibitors, we have expressed them in the Pichia pastoris expression system Using a genetic construct consisting of ... as the kdiss/kass ratio The progress curve method was used to follow the time course of HNE and PR3 inhibition The reagent concentrations were chosen to yield both easily detectable signals and...
... systems Fire Suppression and Seating, flooring Wheel sets Door systems Door systems and HVAC Communications systems; Horns, bells; Security systems Security systems Labels Driving control systems ... Articulation systems Bathroom Integrated soft ware Brake parts Communications systems; Security systems Windows Lighting Security systems Brake systems; Door systems; Electric collector; Sanding systems; ... Truck system and Undercarriage casting Truck system and Wheel sets Communications systems and Security systems Fuel systems Wheel sets Communication systems and Electric generator Brake parts Wheel...
... relative fluorescent units Discussion Competitive versus uncompetitive inhibitors Studies of enzyme inhibitors in open systems have suggested that competitive inhibitors, still a major focus of therapeutic ... localized and systemic sPLA2 response as has been demonstrated for a variety of disorders, including following nervous system lesions (see Introduction) Inflammation and associated oxidative stress will ... enzyme activity in human plasma at the lowest substrate concentrations used This is a classical feature of uncompetitive inhibition because at low substrate concentrations the mass action effects of...
... 7) Analysis of assigned subject areas Analysis of the subject areas assigned to the publications revealed only two involved medical issues in the first ten areas The other eight areas dealt either ... affinity of languages It is possible that states belonging to the former USSR still use Russian as their scientific language after the independency from the Russian government It is also possible ... intensified towards this topic While natural, stationary emission cannot be significantly influenced, a major focus has been given to changes in man-made pollutant emissions Heretofore, there has...
... episodes yearly in the United States [1] On average, each episode is associated with 1.2 days of missed work or classes [1] The cost of treating ambulatory patients with symptoms of dysuria is ... successfully applied to acute illnesses, whereas other quality of life scales, such as the SF-36 Health Survey, are better suited to assess chronic illnesses [4] Higher scores on the Quality of Well-Being ... treatment of acute cystitis [2,3] In most cases, these guidelines suggest treatment based upon symptoms of disease in otherwise healthy adult women and recommend against obtaining culture and sensitivity...
... using software Statistica 7.0 RESULTS AND DISCUSSION Distribution Six species of the genus Anthribus Geoffroy, 1762 are known from the palearctic region (Rheinheimer 2004) The seventh species ... found on the same tree species and further on Salix sp., Fagus sp., Quercus sp and Larix sp (see above) In 2002–2003 (–2005), an outbreak of A nebulosus was observed in several parts of the Czech ... eggs of the scale insects Larvae pupate in this place and emerged adults bite out through the dorsal parts of hosts’ scales (Valentine 1962) The whole development lasts about two months (Silvestri...
... vitro assays (cell adhesion assays, FACS analysis, reporter gene assays) and the statistical analysis PG and AK performed the animal experiments and intravital fluorescence microscopic analysis RV ... its potential to interfere with trafficking of other cells of the innate and adaptive immune system such as lymphocytes, monocytes, dendritic cells and mast cells The answers to these questions ... forward and sideways light-scattering properties and counting of 5,000 events demonstrate the increasing surface expression of Eselectin in response to stimulation with IL-1β (curve shifted furthest...
... at least two similarly treated vital tumor tissues of allografts and orthotopic tumors For the allograft tumor tissue sections mice/group and for the spontaneous tumor model tissue sections 2-4 ... with IR and PTK787 resulted in a similar absolute increase (appr fold) oftumor cell apoptosis in comparison to control tumors (p < 0.001) In comparison to the apoptotic treatment response to IR ... di Tomaso E, Hooper A, Huang P, Huber J, Hicklin DJ, Fukumura D, Jain RK, Suit HD: Responses to antiangiogenesis treatment of spontaneous autochthonous tumors and their isografts Cancer Res 2003,...
... this BST-2 mutant Glycosylation of BST-2 is not required for inhibitionof virus release and sensitivity to Vpu To assess the importance of BST-2 glycosylation for the inhibitionof virus release ... molecular and biochemical studies, and assisted in writing the manuscript EM performed biochemical and FACS analyses and helped with data analysis SK assisted with BST-2 mutagenesis and biochemical ... and datura stramonium lectin (DS lectin) binding assays For glycoprotein analysis of BST-2, cell lysates were prepared as follows: Cells were washed once with PBS and lysed in 300 μl of lysis...
... events leading toapoptosis ([11] Hanauske-Abel et al., in preparation) In phase I the drugs cause a reduction in the expression of HIV proteins, some of which are necessary to prevent apoptosisof ... streptomycin and 8% FBS Quantitation ofapoptosisand viability was performed with a BD FACSCalibur system using Annexin V-PE Apoptosis Detection Kit I (BD Biosciences, San Jose CA) Plasmids pSP-luc+ and ... reverse transcriptase and protease inhibitors Despite the success of combinations of these drugs in highly active antiretroviral therapy (HAART), the emergence of drug-resistant HIV-1 strains that...
... it is not sufficient to deal with classical solutions of differential equations One needs to introduce the notion of weak derivatives andto work in Sobolev spaces, which have become an indispensable ... Press, New York, NY, USA, 1975 SS Dragomir and T M Rassias, Eds., Ostrowski Type Inequalities and Applications in Numerical Integration, Kluwer Academic Publishers, Dordrecht, The Netherlands, ... Brezis, Analyse Fonctionelle, Th´ ory et Applications, Masson, Paris, France, 1992 e F C Cˆrstea andSS Dragomir ı 15 R A Adams, Sobolev Spaces Pure and Applied Mathematics, vol 65, Academic Press,...
... it is not sufficient to deal with classical solutions of differential equations One needs to introduce the notion of weak derivatives andto work in Sobolev spaces, which have become an indispensable ... Press, New York, NY, USA, 1975 SS Dragomir and T M Rassias, Eds., Ostrowski Type Inequalities and Applications in Numerical Integration, Kluwer Academic Publishers, Dordrecht, The Netherlands, ... Brezis, Analyse Fonctionelle, Th´ ory et Applications, Masson, Paris, France, 1992 e F C Cˆrstea andSS Dragomir ı 15 R A Adams, Sobolev Spaces Pure and Applied Mathematics, vol 65, Academic Press,...