biochemical properties of starch phosphorylase
Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... protein, Mx Because of similarities in the molecular architecture of GBPs and members of the dynamin family, GBPs are classified as a part of the dynamin superfamily Although their biochemical characteristics ... been detected in all melanoma and most of lymphoma cell lines tested 1598 In this study, we focus on the biochemical properties of both splice variants of hGBP5, hGBP5a ⁄ b (amino acids 1-586) ... the lack of GMP formation (see Fig S2) The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that of the analogous deletion mutant of hGBP1...
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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc
... characterization of the reversible binding complex between OBP and HNE; (b) investigation of the chemical modifications of OBP as a result of incubation with large excesses of HNE and evaluation of their ... Evaluation of the protective role of OBP against HNE cytotoxicity in a simplified model simulating the nasal epithelium The aim of this experiment was a preliminary evaluation of the protective role of ... peroxidation of unsaturated fatty acids This study was realized through characterization of the binding properties and chemical resistance of OBP to HNE, an alken-aldehyde involved in the pathogenesis of...
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Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt
... the two isoforms of the ADP ⁄ ATP translocase [14–16], the carnitine ⁄ acylcarnitine [17,18], citrate [13] and mitoferrin carriers [19] In the present study, we report the identification of two D ... Approximately 90 mg of purified protein was obtained per litre of culture Functional characterization of recombinant DmTpc1p DmTpc1p was reconstituted into liposomes, and its transport properties were ... Leu5, accumulation of CoA in the matrix; yPTP, phosphate transport carrier; Ndt, NAD+ carrier protein; Rim, pyrimidine nucleotides carrier; YPR011c 1178 The biochemical properties of the recombinant...
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Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
... Ó FEBS 2003 Dimer stability of bacterial starch phosphorylase (Eur J Biochem 270) 2127 important in the physiology of C callunae In light of the fact that interactions apparently ... given conditions Binding of phosphate and sulfate to StP or mutants thereof was determined by using a previously reported procedure in which inhibition of quenching of cofactor fluorescence by iodide ... absence of another open reading frame % 1000 bp upstream of the start codon and % 2000 bp downstream of the stop codon of the StP gene The entire open reading frame for StP consisted of 2388...
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EFFECT OF STORAGE TEMPERATURES ON THE PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES OF HYLOCEREUS POLYRHIZUS
... 23.694% viii TABLE OF CONTENT DECLARATION Page iv ACKNOWLEGEMENT v DECLARED BY vi ABSTRACT vii ABSTRAK vii LIST OF TABLES ix LIST OF FIGURES xi LIST OF PHOTOS xiv LIST OF SYMBOL xv LIST OF APPENDIX ... Trend of Consumer Perception Towards Herbs 2.1.4 Current Trend of Consumption of Herbs 2.2 Herb of Limnophila aromatica 2.2.1The Morphology of Limnophila aromatica 2.2.2 The Uses and Benefits of ... aromatica (Scrophulariaceae) Biochemical SystematicS and Ecology 32(10): 943-947 Bureau of Plant Industry 2005 Medical Plants of Philippines Republic of Philippines ' of Agriculture Department...
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Báo cáo khoa học: "Molecular structure and biochemical properties of lignins in relation to possible self-organization of lignin networks" pdf
... organization of a system, can be and of time function of redundancy (see Annex) Evolution of the organization as a expressed as a be calculated function of time can thus different types of showing ... hypothesis of biochemical heterogeneity of lignins Phenolic acids are known to be bound lignin, especially in the cases of monocotyledons (grasses and bamboos) and Salicaceae (poplars) Ester bonds of ... the = appearance of certain dimensional network Biochemical regularities in the properties Biochemical heterogeneity or inhomogeneity (Monties, 1985) is the second main feature of lignin Characteristic...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... SDS/PAGE and MALDITOF MS of the whole protein Both purified and crude samples of recombinant kynureninase can be stored in the absence or presence of PLP for extended periods of time (> 12 months) ... al [19] in vivo, and this raises the possibility of the existence of two isoforms of the enzyme as discussed by Toma et al [8] The pH optimum of 8.25 is consistent with previously reported experimentally ... suspension cultures of 10 L containing a minimal amount of fetal calf serum (1–2%) until 10 mL of growing cells resulted in a confluent layer when placed in a small Petri dish Infection of the Sf9 cells...
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Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx
... presence of troponins In the absence of troponins, binding of Tms to actin was very weak and only small amounts of Tm were detected in gels after centrifugation (data not shown) The addition of troponins ... of the actomyosin S1 Mg2+ ATPase activity by Tm in the presence of troponin The results are expressed as a percentage of the actin-activated Mg2+ ATPase of myosin S1 obtained in the absence of ... transition, either because of lack of resolution or because the transition was invisible to this probe Thus, the analysis of the melting profile of human Tm was enhanced by the use of different probes...
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Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... investigated the amino-acid sequence and some biochemical properties of the 105-kDa protein In the present study, we discuss the biochemical properties of the 105-kDa protein in the rat brain and ... sequence of the 105-kDa protein To investigate the biochemical properties of the 105-kDa protein, the amino-acid sequence of the protein was determined using a peptide sequencer The peptides of the ... [11,12] However, the biochemical properties of the two proteins have not yet been fully understood until today In 1990, we purified and reported some biochemical properties of the 105-kDa protein...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... enzyme intermediate of a double displacement-like mechanism of the phosphorylase The precedent of sucrose phosphorylase [7–9], mechanistically representing a large class of retaining glycoside ... relative elevation of pKa of PLP phosphate in unliganded CcStP and the apparent lack of perturbation of pKa in the CcStP complex with arsenate The pH dependence of the 31P chemical shift of PLP phosphate ... favor some form of a two-step mechanism, consisting of glucosylation and deglucosylation of a catalytic group on the enzyme, typically a carboxylate of Glu or Asp [5,10] Glycogen phosphorylase...
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Novel biochemical and functional properties of the HPV 16 oncoprotein e6
... (PART 1): BIOPHYSICAL PROPERTIES OF E6 AND E7 33 3.1 Biophysical properties of the E6 and E7 proteins: Previous data and concept of this study 33 3.2 Biophysical properties of the E7 protein: ... modes of interaction of E6 with cellular proteins based upon studies of a new interaction partner 33 - Results (part 1): Biophysical properties of E6 and E7 3.1 Biophysical properties of the ... List of figures Figure 1: Prevalence of different cancers in cancer related deaths in women Figure 2: Picture of the capsid of human papillomavirus Figure 3: Organization of the genome of HPV16...
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Properties of Convolution
... operations Chapter 7- Properties of Convolution 127 2.0 a Original signal Amplitude 1.0 0.0 -1.0 FIGURE 7-3 Example of calculus-like operations The signal in (b) is the first difference of the signal ... curve of the form: sin(x)/(x) , is used to separate one band of frequencies from another c Sinc 0.3 Amplitude Amplitude 0.3 0.2 0.1 0.0 -0.1 -20 -15 -10 Sample number Chapter 7- Properties of Convolution ... affect any of the output signal values As shown by the examples in Fig 7-6, the impulse response of a causal system must have a value of zero for all negative numbered samples Think of this from...
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FOAMING PROPERTIES OF PROTEINS.pdf
... summary of the independent parameters the modified foaming apparatus FOAMING PROPER TIES used far experi- Nature Fixed Diameter Porosity Volume Volume Mass of of sparging disc of sparging disc of ... = Tw Did = = percent of gas initially in 70 ml of foam (gas Flow Rate) - ml/min of nitrogen time to fill the column with foam volume of liquid in the foam initially volume of liquid drained from ... characteristics of protein i.e the foaming capacity of protein and the stability and strength of the foam Foaming ability i.e Gi denotes the amount of gas retained in the foam reflecting the potential of...
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Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
... University of Bari in 1979 University Researcher at the of Engines and Energy Institute of the Faculty of Engineering at the University of Bari from 1983 to 1987 Associate professor of Technical ... measure of conductance The analyses, done in the laboratory of Technical Physics of the Polytechnic of Bari, were performed on some samples of calcarenite sandstone and calcareous stone of Fasano ... typologies of building wall: a wall in blocks of calcarenite sandstone assembled with mortar, typical of the constructions of "Sassi of Matera," and a masonry made with dry calcareous stones of Fasano,...
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Effects of time of heatsetting on the tensile properties of ingeo™ poly (lactic acid) (PLA) fabric
... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension ... Increasing time of heatsetting had effect on tensile properties of PLA at varying times of 30s to 90s at temperature of 130°C Optimum effect on tensile properties occurred at times of 30 – 45s PLA ... [PLA] Figure Mean [Ā] of effect of time of heatsetting on the tensile extension EM [%] of Ingeo™ Poly (lactic acid) 3.2 Linearity of load extension curve LT [-] The linearity of load extension curve...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... of organic acid and reduced levels of ammonia after compost processing [41, 42] Table Characterization of yard waste compost samples Specifications YWCa-I YWC-II Location Bottom of heap Top of ... reports, however, lack information on the biochemical and kinetic properties of the secreted enzymes Such information may be useful in gaining better understanding of the lignocellulose biodegradation ... Hackensack, NJ) During extrusion, a screw speed of the extruder of 100 rpm and a barrel temperature of 100°C was maintained [28] 2.3 Sample collection Samples of yard waste compost (YWC) and finished...
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Physicochemical properties of polluted water of river Ganga at Varanasi
... pH Because most of the chemical and biochemical reaction are influenced by the pH it is of great practical importance The adverse affect of most of the acids appear below and of alkalis above ... quality criteria of an aquatic system In the system where rate of respiration and organic decomposition are high ,the DO values remain lower than those of system where the rate of photosynthesis ... growth and activities of microorganism 3.6 Biological Oxygen demand It has been used as a measure of the amount of organic materialism an aquatic solution which support the growth of microorganism...
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Properties of American Derivative Securities
... owner of the derivative security should exercise it If he does not, then the seller of the security can immediately consume f Vk ! , + r IE Vk+1jF k ! and still maintain the hedge 6.2 Proofs of ... maintain the hedge 6.2 Proofs of the Properties Let fGk gn=0 be a sequence of non-negative random variables such that each Gk is F k -measurable k Define Tk to be the set of all stopping times satisfying ... Lemma 2.18 Proof: Take Vk Gk for every k Tk to be the constant k Lemma 2.19 The process f 1 + r ,k Vk gn=0 is a supermartingale k Proof: Let attain the maximum in the definition of Vk+1 , i.e.,...
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