bcgin dna induces up regulation of cd44hi cd62llo expression in pulmonary t lymphocytes
Báo cáo sinh học: "Improve protective efficacy of a TB DNA-HSP65 vaccine by BCG priming" pdf
... present study, we investigated the influence that the order and route of BCG vaccination in combination with DNA- HSP65 vaccine has on the induction of protective immunity against TB http://www.gvt-journal.com/content/5/1/7 ... immunization strategies Initially, we tested the ability of different combinations of prime-boost strategies to induce protection against M tuberculosis and compared the results with those obtained ... relation to BCGsc, BCGin and DNA- HSP65 groups, on days 30 and 70 after infection (Table 2) BCGin/ DNA induces up- regulation of CD44hi/ CD62Llo expression in pulmonary T lymphocytes Two lymphocyte...
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Báo cáo Y học: Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation doc
... TGTCCCTGCTAGTTGTCATTTGG ACGACCACTTTGTCAAGCTCATT TGAGGTCCACCACCCTGTTG TCCTGAAACGCCTTCGGAAGAG CCATTGGGTTGAAGGCATTCG ACAAGGCCCCTGGCTGCT CCTGTCAAAAGAATAAACAGCGGTT CACTGTTCCCTCAGCCGAGGAC CCAACTCCTGATCGGCAGAAGC ... start site (at nucleotide 1633) of transcript 5¢-A This start site is part of an octanucleotide that is highly similar to the consensus transcriptional initiator sequence [31, 32] The initiator ... would bring the total number of 5¢ noncoding exons up to four At least four promoters, here called A–D, are involved in the initiation of transcription of porcine a1,3GalT Alternative start site usage...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... GCACCCAACATATACAG CATTCA AGCCTCGCTCTTGGCAG GTACTCAGT [GenBank: NM_173841] TNF-α CGAACATCCAACCTTCC CAAAC TGGTGGTCTTGTTGCTTA AAGTTC CCAATCCCTTTATTACCC [GenBank: NM_000594] TNF receptor AATTCTGGCTTCTAGTCT ... AATTCTGGCTTCTAGTCT GGT AACGTGCCAGTGTGGAG TGA TTCAGTCCCACTCCAGG CTTCACCC [GenBank: NM_001065] TNF receptor PDAR PDAR PDAR MMP-3 TGAAGAGTCTTCCAATC CTACTGTTG CTAGATATTTCTGAACAA GGTTCATGC TTTGCTCAGCCTATCCAT [GenBank: ... GTTGGGCGGCAGGTAC TG CGACGTCATCTCCAACA T [GenBank: NM_000346] IL-1α AAGAGGGAAGTTTGCTT GATTAAGG GAGGATCAAGACTTCTTT GTGCTC ACCACTGTTCTCTTCTCT ACCCTGCCC [GenBank: NM_000575] IL-1β CGGCCACATTTGGTTCT AAGA...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
... ccaagggagagtggtcaggt ggcaacaaggtagagaggc 317 IL-1β atcactcattgtggctgtgg gtcgttgcttggttctcct 322 IL-10 tgctatgctgcctgctctta gctccactgccttgctctta 405 GAPDH accacagtccatgccatcac tccaccaccctgttgctgta 452 ... group that demonstrated an important role for TNF-receptor in the septic course In that study, induction of sepsis by CLP resulted in a mortality rate of nearly 100% in TNF-receptor knock-out ... interpretation, performed the experiments statistical analysis and drafted the manuscript FH and CK participated in the interpretation of data MG carried out the design of the study, scored the...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf
... one-third of the PKI-inhibited activity was released into the extract, indicating that the precipitated Cb2 colocalized with Ca1 on RIa and RIIa in a ratio of : Of the total PKA kinase activity, ... activity in Cb2-transfected 29 3T cells was precipitated Taken together it is therefore reasonable to assume that Cb2 activity may constitute more than 20% of total PKA activity in T- cells It ... This demonstrated that precipitates extracted with 8-CPTcAMP contained cAMP-inducible PKA activity that could be inhibited by protein kinase inhibitor (PKI) In the presence of cAMP approximately...
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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot
... 5¢-GGCTTAGAGCTTAATTGCTGAATCTGGTGCTCGCCTGATAAATTGTGTCGAAATCCGCGATCTGCTCC ATGTTACTTAGCCGGAACGAGGCGGATTCGCGATTCCTTGATGCCTATGGTGA-3¢ 5¢-GGCTTAGAGCTTAATTGCTGAATCTGGTGCTCGCCTGATAAATTGTGTCGAAATCCGCGACCTGCTCC ATGTTACTTAGCCGGAACGAGGCGGATTCGCGATTCCTTGATGCCTATGGTGA-3¢ ... ATGTTACTTAGCCGGAACGAGGCGGATTCGCGATTCCTTGATGCCTATGGTGA-3¢ 5¢-GCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAATTTATCAGGCGA-3¢ 5¢-TCGCCTGATAAATTGTGTCGAAATCCGCGATCTGCTCCATGTTACTTAGCCGGAACGAGGC-3¢ 5¢-TCGCCTGATAAATTGTGTCGAAATCCGCGACCTGCTCCATGTTACTTAGCCGGAACGAGGC-3¢ ... CLS GTS GCS 61 61 61 16 16 16 5¢-TCACCATAGGCATCAAGGAATCGCGAATCCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCG ATTTCGACACAATTTATCAGGCGAGCACCAGATTCAGCAATTAAGCTCTAAGCC- 3¢ 5¢-GGCTTAGAGCTTAATTGCTGAATCTGGTGCTCGCCTGATAAATTGTGTCGAAATCCGCGATCTGCTCC...
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Báo cáo khoa học: Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells pptx
... )151 to )129 5¢-CCCGTCGTGGTATTGGTCTGGGC-3¢ )89 to )64 5¢-CGCGTCTCGGGGGAGTAGTCTGTACC-3¢ )89 to )64 5¢-CGCGTCTCGGTTTAGTAGTCTGTACC-3¢ )61 to )36 5¢-CGTGATGTCCCCGCCCCGGTTCCCAG-3¢ )61 to )36 5¢-CGTGATGTCCCAAACCCGGTTCCCAG-3¢ ... unknown factors interact with Sp1 or Sp3, facilitating their binding to GC-I and enhancing their transcriptional activity On the basis of these results, we cannot rule out the possibility that other ... Early studies indicated that Sp1 is responsible for recruiting TATA-binding protein [21] and guiding transcriptional initiation [22] at promoters without a TATA box Recent studies showed that Sp1...
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Báo cáo y học: "Methotrexate enhances the anti-inflammatory effect of CF101 via up-regulation of the A3 adenosine receptor expression" doc
... compared with the control group and each of the other treatment groups (Figure 1a) In the therapeutic treatment study, in which all of the treatments were initiated at the onset of disease, clinical ... strongly support the notion that the anti-inflammatory effect of MTX is attributed more to adenosine than to tetrahydrolate MTX increases the extracellular concentration of adenosine, where it is ... with the vehicle-treated group In the CF101-treatment and the MTX+CF101-treated groups, receptor downregulation was noted, supporting previous studies demonstrating that receptor downregulation...
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Ceftriaxone-induced up-regulation of cortical and striatal GLT1 in the R6/2 model of Huntington’s disease pps
... loss of transporter function well in advance of protein down -regulation Thus, ceftriaxone is capable of overcoming a deficit in GLT1 function It is interesting in this regard that palmitoylation, ... indicating that our treatment protocol is within normal limits for this drug Nevertheless, it is interesting that ceftriaxone increased cortical and striatal GLT1 expression in both R6/2 and WT mice ... symptomatic [17] Effects of ceftriaxone treatment in cortical and striatal GLT1 expression Although saline-treated R6/2s showed no loss of either cortical or striatal GLT1 relative to WT at weeks...
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Báo cáo y học: " Cellular stress-induced up-regulation of FMRP promotes cell survival by modulating PI3K-Akt phosphorylation cascades" pdf
... and its stem and loop structure is as follows: CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT (2) in vitro shRNA virus transduction shRNA virus was used at 50 multiplicity of infection ... an increase in protein level of FMRP (Figure 1C-D) suggesting that etoposide treatment results in a transcriptional up- regulation of Fmr1 mRNA that leads to increased FMRP protein level In this ... activated Ras, integrin, vitronectin, and hepatocyte growth factor [48] Attractive points are among them STAT protein nuclear translocation [9], RAS, and integrin protein signal transduction [7],...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
... medium in triplicate Then they were put into a 24-well culture plate containing 500 μl tumor supernatants 12 h later, the inserts were removed and washed with PBS, fixed, stained, rinsed with water, ... cell supernatants cultured with HUVEC, but not 1640 medium Notably, BGC/STC supernatants showed an augmentation effect on the tube network while BGC/shSTC supernatants resulted in shorter and ... Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the nucleus of tumor cells (E) Quantification of PCNA expression( the Integrated Optical Density (IOD) of PCNA) by...
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Báo cáo y học: " Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium" doc
... the linear range of detection The contrast was inverted, the pixel intensity of each band determined, and the background pixel intensity for a negative area of the film of identical size subtracted ... proteincontaining supernatant was collected The protein concentrations were assessed using a bicinchoninic acid (BCA) protein concentration kit (Pierce, Rockford, IL) Equal concentration of protein ... Interestingly, the effect of nicotine was blocked both by the nicotinic antagonists and by the GABA-A receptor antagonists This suggests that the sequential activation of nicotinic signaling followed by...
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Up regulation of c EBPa in hepatocellular carcinoma is correlated to poorer prognosis
... B-actin Forward Primer (5'-3') CGAGTTCCTTCAACATGCACT CAGTCACAAGTCGTTTTGCCT GGGGTACTTTATCACGCCCTG TGTATACCCCTGGTGGGAGA GGACTTCGAGCAAGAGATGG Reverse Primer (5'-3') TTTGCGGTCAGTTCCTGAGC AATGGGCTGGGAATAGTAGGT ... AATGGGCTGGGAATAGTAGGT GGGAATCCCGTTCTCATCAGA TCATAACTCCGGTCCCTCTG AGCACTGTGTTGGCGTACAG The following conditions were set up in the Roche LightCycler 480 machine: an initial denaturation step of min, followed ... Representative staining patterns of C/EBPα in HCC tissues Nuclear staining of the hepatocytes in the tissue sections were scored between 0-3, with indicating negative staining and 1-3 positive staining...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... observed These uncoupling effects were the result of overexpression of the recombinant proteins leading to a compromised mitochondrial integrity rather then to an intrinsic property of the proteins This ... ancillary protein(s) Alternatively, it is possible that certain cofactors necessary for the uncoupling are missing in the cell culture system In this respect, it is relevant to mention that UCP2 and ... and inhibit the intrinsic uncoupling activity of hUCP3, the digitonin treated cells were extensively incubated with the resin Dowex-K21 This extracting procedure was found to effectively remove...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... findings, together with previous reports in the literature regarding other immune cell types at different stages of maturation and activation, indicate that this signaling system might be regulated in ... against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh ... endocannabinoidmediated T cell–dendritic cell communication Finally, the presence of relatively high amounts of PalEtn in both immature and mature dendritic cells might indicate that the strong anti -in ammatory...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... Benz et al N-terminus, anchoring it in the IEM, whereas the C-terminus of the protein projects into the stroma (Fig 1) [34] Two motifs can be located in the C-terminal half of the stromal domain: ... appear to mediate the interaction with Tic110 and not with the chaperone partner (Hsp93), which is in contrast to the function of these motifs in Hop and Hip [40,42,43] Interestingly, binding of Tic40 ... Tic40 to Tic110 is favoured when the transit peptide-binding site of Tic110 is occupied by incoming preprotein, but interaction with Tic40 appears to decrease the affinity of Tic110 for the transit...
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Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf
... complex, the MT1-MMP is inactive as a result of its interaction with the N-terminal part of TIMP-2, whereas the C-terminal part of the inhibitor binds to the HPX domain of proMMP-2 Another MT1-MMP ... PGs This compartmentalization regulates the MMP activity by locating and concentrating them close to or on potential substrates The interaction with their binding partners varies in strength, ... domain of proMMP-9 can bind to both hemin and b-hematin, which results in an autocatalytic truncation of parts of the enzyme’s prodomain [68] The truncation in the presence of hemin results in two...
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Báo cáo Y học: Regulation of peptide-chain elongation in mammalian cells pptx
... although it might affect its interaction with eEF1A or other proteins Another GEF involved in translation initiation provides a precedent for this – the phosphorylation of two sites in the extreme ... control of translation initiation This involves the phosphatase-binding protein Tap42 [66], which binds to PP2A and related enzymes TOR signalling promotes the interaction of Tap42 with these phosphatases ... phosphorylation sites in the regulation of eEF2 kinase, and the identification of the kinase(s) acting at the novel mTOR-regulated sites Indeed, identification of these kinases may well provide important...
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Report on the Regulation of Reproductive Cell Donation in the European Union potx
... considers it essential to encourage Member States to incorporate into their national legislation the principle of voluntary and unpaid donation It regards the paying of substantial fees to obtain human ... (published in G.U 03.12.05) set up a National Registry of authorised institutions that can apply techniques of medically-assisted procreation This Registry is held by the National Institute of Health ... the Act of Status and Rights of Patients requires that mutual understanding be a requisite for patient treatment, explicit consent of the patient in everyday healthcare procedures is not required...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... of the proregion in controlling activation of the enzyme, it appears that the CT-peptide might be implicated in regulating enzyme activity This adds an additional level of complexity to the regulation ... prerequisite for PC1 ⁄ enzyme activity in the constitutive pathway, and that the CT-peptide appears to act as an inhibitor Direct inhibition of PC1 ⁄ enzymatic activity by a CT-peptide has been tested ... corresponds to incubation of the enzyme with the substrate in the absence of any CT-peptide [CT-peptide] ¼ 3484 When PC1 ⁄ activity was examined at two different concentrations of substrate in the presence...
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