arima 0 1 1 in place of arima 0 0 1
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... A589G_G590C A589G_G590C T 10 0 G_C 10 2 A T 10 0 G_C 10 2 A T 205 G_C 206 A 18 8 -1 90 18 8 -1 90 18 8 -19 1 18 8 -19 1 RKT RKT RKTT RKTT PKA PKC PKC PKA A568G_C569A_A570C A568G_C569A_A570C A571G_C572A A571G_C572A 18 9 -19 2 KTTS ... 5 10 confocal microscope Western Analysis β-tubulin (1: 10 0 0), Lamin-A (1: 10 0 0), Phospho- PKA (1: 10 0 0) and PKC substrate (1: 200 ) antibodies were obtained from Cell Signaling (Beverly, MA) Claudin -1 ... and Furukawa Y Involvement of claudin -1 in the beta-catenin/Tcf 10 1 signaling pathway and its frequent upregulation in human colorectal cancers Oncol Res 200 1; 12 (11 -12 ): 469-476 19 Dissanayake...
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A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part 1
... Personal Information The following graph shows the age of teachers: 50 40 30 20 10 12 % below 29 years 46% 30- 39 years 41% 40- 49 years 1% 50+ years Figure 2.6.2 .1. 1 Age of teachers Teaching experience ... years18% 6- 10 years 27% 11 -15 years 33% 16 - 20 years Figure 2.6.2 .1. 2 No years of ELT experience 3% 20+ years 10 Approximately one third ( 31% ) have received some form of in- service training, mostly in ... training More than two- thirds of teachers have been teaching for 10 years or less and one- third of teachers have been teaching for five years or less 35 30 25 20 15 10 3%2-years 16 % 2-5 years18%...
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Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx
... Solution: F1u = sin ( γ − α ) F1 sin ( 1 80 deg − γ ) sin ( γ − α ) F 1u = F1 sin ( 1 80 deg − γ ) F 1u = 205 N F 1v sin ( α ) = F 1v = F F1 sin ( 1 80 deg − γ ) sin ( α ) sin ( 1 80 deg − γ ) F 1v = 1 60 ... 2 50 N F = 1 50 N θ = 30 deg θ = 30 deg θ = 10 5 deg Solution: F 1v sin ( θ ) = F1 sin ( θ ) ⎛ sin ( θ ) ⎞ ⎜ ⎟ ⎝ sin ( θ ) ⎠ F 1v = F F 1v = 12 9 N F 1u sin ( 1 80 deg − θ − θ ) F 1u = F1 = F1 sin ... Solution: F = G m1 m2 d F = 10 . 0 nN W1 = m1 g W2 = m2 g W1 W1 = 78.5 N W2 = 11 8 N = 7.85 × 10 W2 F = 1. 18 × 10 F 10 Problem 1- 17 Using the base units of the SI system, show that F = G(m1m2)/r2 is a...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... containing 1% SDS, 10 mm EDTA, 50 mm Tris ⁄ HCl, pH Lysates were centrifuged at 14 00 0 g for 10 at °C, and the supernatant obtained was diluted 10 - fold using IP buffer [0. 01% SDS, 1. 1% Triton X- 10 0 , ... antibodies against actin (mouse monoclonal, 1: 400 0, Sigma), lamin C (rabbit polyclonal, 1: 10 0 0, Abcam), MnSOD (rabbit polyclonal, 1: 200 0, Sigma) and GAPDH (rabbit polyclonal, 1: 10 0 0, Abcam) were ... ( 200 4) Prediction and functional analysis of native FEBS Journal 276 ( 200 9) 7386–7399 ª 200 9 The Authors Journal compilation ª 200 9 FEBS D Mizgalska et al 10 11 12 13 14 15 16 17 18 disorder in...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... KG1 and Jurkat cells were maintained in RPMI -16 40 medium (Sigma) Each medium contained 10 % heat-inactivated fetal bovine serum, penicillin G ( 10 0 UÆmL )1) , and streptomycin sulfate ( 10 0 lgÆmL )1) ... accumulation in the lungs of mice lacking heme oxygenase-2 J Clin Invest 10 1 , 10 0 1 10 1 1 Adachi T, Ishikawa K, Hida W, Matsumoto H, Masuda T, Date F, Ogawa K, Takeda K, Furuyama K, Zhang Y, et al ( 200 4) ... resetting of HO -1 expression In contrast with HO -1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines examined [15 ,19 ,37] Such a mode of regulation of...
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Tài liệu Báo cáo khoa học: The Ets transcription factor ESE-1 mediates induction of the COX-2 gene by LPS in monocytes doc
... ( 200 0) Involvement of CCAAT enhancer binding protein transcription factors in the regulation of prostaglandin G ⁄ H synthase expression by interleukin -1 in osteoblastic MC3T3–E1 cells J Bone Miner ... were infected with adenovirus for h in serum-free medium using a multiplicity of infection of 300 After infection the cells were washed with medium and incubated for 16 h in DMEM containing 10 % ... regulating COX-2 promoter activity in at least some cell types [7 ,11 ] and NF-jB cooperates with ESE -1 in transactivating the iNOS promoter in endoFEBS Journal 272 ( 200 5) 16 76 16 87 ª 200 5 FEBS...
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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx
... after fMLP ( 10 0 nM, 10 min) stimulation of TNFa-primed ( 200 UÆmL )1, 30 min) cells The inhibitors wortmannin ( 10 0 nM) (A, B) and brefeldin A ( 10 0 lgÆmL )1) (C, D) or vehicle were added 10 before stimulation ... at ) 50 °C [ 21] Thin sections mounted on nickel film were incubated in primary antibodies to PLD diluted : 10 0 in NaCl/Pi containing 2% BSA, 0. 1% Triton X- 10 0 and 0. 1% Tween 20 (w/v/v/v) for 16 h ... fMLP ( 10 0 nM, 10 min) stimulation of TNFa-primed ( 200 UÆmL )1, 30 min) cells Data represent mean ± SEM from three experiments performed in triplicate ***P < 0. 0 01 , significant inhibition of MPO...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... trithorax-related SET domain protein Proc Natl Acad Sci USA 98, 12 902 12 907 Tenney K & Shilatifard A ( 200 5) A COMPASS in the voyage of defining the role of trithorax ⁄ MLL-containing complexes: Linking leukemogensis ... Journal 272 ( 200 5) 3 505 –3 511 ª 200 5 FEBS A M Rojas et al 12 Shearn A (19 89) The ash -1, ash-2 and trithorax genes of Drosophila melanogaster are functionally related Genetics 12 1, 517 –525 13 Mazo AM, ... Dawid IB (19 90) The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains Proc Natl Acad Sci USA 87, 211 2– 211 6 14 Tripoulas...
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Mobil 1 is original equipment in some of the world’s finest vehicles, including potx
... 2 10 , 000 kilometers 0W- 40 3 50 300 Iron Level 3 50 Brand new 200 suggested alert level 1 50 10 0 50 00 1 ,0 23 00 0, 21 00 9, 18 00 8, 16 00 7 ,0 14 00 6, 12 00 5, 10 00 ,0 84 00 ,0 63 00 ,0 42 00 ,0 ... maximum 50% 40% 30% 20% 10 % 0% 00 1 ,0 23 00 0, 21 00 ,0 18 00 ,0 16 00 7 ,0 14 00 ,0 00 00 ,0 12 10 ,0 00 00 ,0 ,0 84 63 42 00 ,0 21 Test Kilometers While conventional oils may increase in viscosity ... should remain low indicating good protection against engine wear 00 0, 21 00 00 ,0 ,0 18 16 00 7 ,0 14 00 ,0 12 00 00 ,0 ,0 10 84 00 ,0 00 00 ,0 63 42 ,0 21 Test Kilometers When using Mobil 1, oil...
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Báo cáo khoa học: Dynamic reorganization of the motor domain of myosin subfragment 1 in different nucleotide states docx
... ANNSer1 81 ANNSer1 81/ IAFCys 707 FHSLys553/ ANNSer1 81 IAEDANSCys 707 IAEDANSCys 707 / FHSLys553 lM 17 lM 30 lM 0. 05 0. 25 0. 45 0. 08 0. 20 0. 40 0 .05 0. 15 0. 27 0. 04 0. 09 0. 17 0. 19 0. 36 0. 61 0. 19 0. 27 0. 33 ... determined at pH 7 .0 using the absorption coefcients of 8 400 M )1 cm )1 at 3 61 nm [22], 6 10 0 M )1 cm )1 at 336 nm [23], 68 00 0 M )1 cm )1 at 495 nm [24] and 55 00 0 M )1 cm )1 at 496 nm (determined for ... Somogyi, B ( 200 1) 9-Anthroylnitrile binding to serine -18 1 in myosin subfragment as revealed by FRET spectroscopy and molecular modeling Biochemistry 40, 14 806 14 811 41 Hiratsuka, T.K.T ( 200 3) Chemical...
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Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot
... and in vitro with NPR1/ NIM1 [27–29,32,33] NPR1/NIM1 has been identified as a key regulator of SAR in Arabidopsis acting downstream of SA in the SAR signaling pathway [ 10 , 11 , 21] , and analysis of ... Plant-Microbe Interact 13 , 19 1– 202 30 Niggeweg, R., Thurow, C., Kegler, C & Gatz, C ( 200 0) Tobacco transcription factor TGA2.2 is the main component of as-1binding factor ASF -1 and is involved in salicylic ... progeny plants of lines 2 01 – 10 or 2 01 –4 Equal amounts of protein from disks incubated for days on water (0) , on mM (1) , or on mM SA (5) were analyzed for the accumulation of PR -1 proteins by immunodetection...
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Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin ppt
... immobilized at 1 20 ngÆlL )1 in 10 mM sodium acetate buffer (pH 4.8), resulting in approximately 400 0, 200 0, and 17 00 0 immobilized resonance units, respectively In all SPR experiments, HBS buffer [ 20 mM ... continuously monitored by injecting buffer alone (beyond 1 50 s) (B) Again, 10 lL 200 nM rIIA was injected directly followed by 40 lL 800 nM active PAI -1 (ÆÆÆ), 300 nM VN (—), 200 nM latent PAI -1 ... (t1/2) of PAI -1 inhibition (A) Residual activity was monitored during the inhibition of 15 nM human thrombin by 1. 5 lM PAI -1, in the absence (d) or presence of 30 nM (s), 50 nM (j), 10 0 nM (h), 400 ...
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HOMINOID EVOLUTION AND CLIMATIC CHANGE IN EUROPE VOLUME 1 The Evolution of Neogene Terrestrial Ecosystems in Europe ppt
... MnO 0. 01 0. 04 0. 02 0. 04 0. 03 0. 04 0. 03 sd 0. 06 0. 03 0. 01 0. 04 0. 03 0. 03 0. 02 MgO 0. 01 0. 01 0. 00 0 .02 0. 01 0. 03 0. 02 sd 0. 33 0. 19 0. 23 0. 22 0. 22 0. 28 0. 21 CaO 0. 02 0. 01 0. 00 0 .05 0. 01 0. 06 0. 02 ... 4 .06 4 .14 Fe2O3 0. 06 0. 06 0. 06 0. 18 0. 35 0. 37 sd 0. 31 0. 18 0. 21 0. 19 0. 19 0. 19 MnO 0. 06 0. 01 0. 01 0. 02 0. 02 0. 02 sd 0. 05 0. 07 0. 08 0. 16 0. 16 0. 16 MgO 0. 01 0. 01 0. 01 0. 02 0. 02 0. 02 sd 0. 31 0. 18 ... 12 .73 11 .42 11 .19 11 .12 10 . 41 11. 11 10 . 62 Al2O3 0. 10 0.26 0. 22 0. 19 0. 23 0. 21 0. 24 sd 1. 69 2.48 2. 81 3 .19 4.43 4.56 4.62 Fe2O3 0. 06 0. 06 0. 06 0. 10 0 .06 0. 33 0. 12 sd 0. 05 0. 08 0. 10 0 .11 0. 15 0. 17 ...
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Báo cáo khoa học: Tumor suppressor p16INK4a: Downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model pptx
... 1 20 0 200 400 FL2-H 600 12 40 80 1 20 1 60 400 FL2-H 200 200 400 FL2-H 16 600 200 400 FL2-H 600 31 10 20 30 40 50 60 0 Counts p16-3 0 40 80 1 2 01 60 200 Mock 30 60 9 01 20 1 50 50 10 0 1 50 200 A 50 10 0 ... DNA 22 60 17 0 0 10 30 20 40 60 80 10 0 1 20 60 90 30 Counts 1 20 E 90 1 20 1 50 B 20 40 60 80 10 0 1 20 A Mock 200 400 DNA asG3-A 200 400 DNA asG3-B Fig Upregulation of Gal-3 in clones changes anoikis ... 2 01 0 The Authors Journal compilation ª 2 01 0 FEBS p16INK4a downregulates anti-anoikis effector Gal-3 Time (h) 200 400 FL2-H 600 200 400 FL2-H 600 12 20 40 60 80 0 600 200 400 FL2-H 600 30 60 90...
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Báo cáo khoa học: Adaptation to G93Asuperoxide dismutase 1 in a motor neuron cell line model of amyotrophic lateral sclerosis The role of glutathione doc
... comparing the basal levels of the various cell lines ( P < 0. 01, P < 0. 0 01 ) or the effect of t-BHQ in each cell line (**P < 0. 01, ***P < 0. 0 01 ) and in the different cell lines (dP < 0. 05, ddP < 0. 01, ... are given as mean ± SEM of three independent experiments DP < 0. 05, DDP < 0. 01 versus tTA- 40 P < 0. 05, P < 0. 01, P < 0. 0 01 versus highWT-tTA hhP < 0. 01, hhhP < 0. 0 01 versus lowG93AtTA (One-way ANOVA ... lgÆmL )1) or without ()) dox, at the fourth passage Values are given as mean ± SEM of four independent experiments DP < 0. 05, DDP < 0. 01, DDDP < 0. 0 01 versus tTA- 40 (dox)) sP < 0. 05, sssP < 0. 0 01 ...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... Foundation of China (nos 306 708 45 and 307 707 87), the ‘973’ Program of China (nos 200 8CB 517 402 ) and the Hebei Province Natural Science Foundation (nos C 200 600 0 814 and C 200 500 0722) References Pfeffer ... specificity Oncogene 20, 2438–2452 15 Shaulian E & Karin M ( 200 1) AP -1 in cell proliferation and survival Oncogene 20, 23 90 2 400 16 van Dam H & Castellazzi M ( 200 1) Distinct roles of Jun : Fos and ... Free probe C Ang II 0. 5 12 h IP: c-Jun AP -1 binding sequence No antibody Input Ang II AG4 90 – – – 3h + IP: STAT5B STAT5B binding sequence FEBS Journal 276 ( 200 9) 17 20 17 28 ª 200 9 The Authors Journal...
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Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung fibroblasts is specifically mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot
... undistinguishable from those of untreated cells and were taken as a control *P < 0. 05 as compared to stimulation with PGE2 in the presence of H-89 10 0 10 1 10 2 10 3 10 4 FL1-H Fig In uence of inhibition ... compilation ª 200 8 FEBS E Sokolova et al PAR -1 downregulation by EP2 A B control 40 80 1 20 1 60 200 CHX the in uence of inhibition of protein synthesis on PGE2-induced downregulation of PAR -1 level Cells ... regulator of the PAR -1 gene and a competitor of AP-2 for binding to the regulatory region of the PAR -1 gene [19 ,29] Interestingly, in the human alveolar epithelial A549 cell line, Sp1 inhibition, as in...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... (Nunc) N18TG2 cells (DSMZ ACC 10 3 ) were cultured in Dulbecco’s MEM, supplemented with 10 % fetal bovine serum (GibcoBRL), 10 0 lM 6-thioguanine (Sigma), 10 0 lgÆmL )1 streptomycin, and 10 0 lgÆmL )1 penicillin ... performed in a volume of 50 lL with 10 0 lg of F -11 cell particulate according to the manufacturer’s instructions Non transfected and rVRL -1 or rVRL -1- CT-GFP transfected F -11 cells were used 30 lg of ... supplemented with 20% fetal calf serum (GibcoBRL), 2% hypoxanthin/thymidine/ aminopterin supplement (Biochrom KG), 10 0 lgÆmL )1 streptomycin, and 10 0 lgÆmL )1 penicillin at 37 °C in a humidified atmosphere...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... 1. 30% 10 1 10 2 CD8 FITC KW 03 1 203 . 01 5 10 3 10 4 10 0 2. 50% 10 1 10 2 CD8 FITC KW 03 1 203 . 01 8 10 3 10 4 4 .17 % MART -1 10 0 10 1 10 2 CD8 FITC 10 3 10 4 10 0 10 1 10 2 CD8 FITC 10 3 10 4 10 0 10 1 10 2 CD8 FITC 10 3 10 4 ... Cells/well TIL/PBMC 80 1/ 5 ,00 0 53 57 15 40 1/ 10 , 000 27 30 14 20 1/ 20, 000 15 17 1/ 50, 000 1/ 10 0 ,00 0 200 1/ 500 86 84 28 10 0 1/ 10 0 0 52 59 19 50 1/ 200 0 37 38 11 25 1/ 400 0 22 31 12 1/ 800 0 13 10 PBMC Low TIL ... MART -1 0. 07 0. 07 0. 42 0. 04 0. 02 0. 49 0. 02 0. 02 0. 47 0. 05 0. 02 0. 43 0. 04 0. 02 0. 54 0. 12 0. 02 0. 59 0. 03 0. 04 0. 40 0 .13 0. 02 0. 58 0. 24 0. 07 0. 48 10 0 .07 0. 06 0. 63 11 0. 04 0. 07 0. 82 12 0. 07 0. 02 0. 55 13 ...
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báo cáo hóa học:" Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma" doc
... UCHL1 B MZ1851RC MZ2862RC 10 M H2O control 5μM 1 M untreated ß-actin DAC MZ1851LN UCH-L1 10 M 5μM 1 M ß-actin 10 M extent of methylation bisulfite DNA sequencing of the respective UCHL1 promoter ... UCHL1 promoter DNA) to 11 - 13 fold in the RCC cell line MZ1851LN (strong methylated UCHL1 promoter DNA) to 11 - 18 fold in the RCC cell line MZ2862RC (partially methylated UCHL1 promoter DNA) 10 0 ... GATCGGTTCGGTTTTGTTTTTTTTTTTGTATTTGTTTTT -3’ 10 M 22 CpG-dinucleotides 5μM coding sequence 10 M -3' +1 1μM +13 5 1 M ATG untreated -1 30 5'- Restoration of UCHL1 expression in RCC by treatment with DAC To confirm that UCHL1 promoter...
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