animals fructose rich diet and green tea extract
Báo cáo y học: "IFN-gamma regulation of ICAM-1 receptors in bronchial epithelial cells: soluble ICAM–1 release inhibits human rhinovirus infectio" pptx
... expression of both mICAM-1 and sICAM-1 forms at the protein and gene level; and (b) to examine the consequences of IFN-γ cell preconditioning on subsequent HRV binding and infection To ensure that ... (HRV-14 and HRV-1b) were purified using a sucrose gradient 20 μg/ml RNase A (Sigma, UK) was added to the viral suspension and incubated at 35°C for 20 mins 1% sodium sarkosyl (Sigma-Aldrich, UK) and ... cells were then washed times in PBS and standard SABM medium was replaced on all cell monolayers to sustain cell growth The gene and protein expressions of membrane and soluble ICAM-1 forms were measured...
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Báo cáo lâm nghiệp: "Yellow-cedar and western redcedar ecophysiological response to fall, winter and early spring temperature conditions" pdf
... and rooted at Cowichan Lake Research Station under standard cultural practices [40] Five random rooted cuttings from each clone were transplanted into gallon pots and grown under a standard greenhouse ... populations had an overall height and diameter of 31.4 cm and 3.8 mm, respectively and the yellow-cedar populations had an overall height and diameter of 28.6 cm and 3.7 mm, respectively 2.3 Shoot ... /C 2⎠ ⎩ ⎭ where T1 and T2 are the conductivity of treatment tubes after freezing and after boiling, respectively, and C1 and C2 are the conductivity of control tubes before and after boiling,...
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Báo cáo lâm nghiệp: " Release of oxalate and protons by ectomycorrhizal fungi in response to P-deficiency and calcium carbonate in nutrient solution" pptx
... mycelia from P500 and P20 media of 1.2, 1.3 and 1.5 for S collinitus 22, S collinitus and R roseolus, 818 J.-C Arvieu et al Table I Contents of oxalate, total or fungus associated, and calcium from ... Semer-Olsen A.R., Automatic methods for determining nitrate and nitrite in water and soil extracts, Analyst 95 (1970) 514–518 [11] Hoffland E., Findenegg G.R., Nelemans J.E., Solubilization of rock ... media were called P20, P100 and P500, respectively The fourth medium, referred to as Pi + CaCO3 medium, contained 500 µM NaH2PO4 and g·L–1 of CaCO3 solid phase instead of CaCl2 The mineral was...
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Báo cáo lâm nghiệp: " Radial growth of mature pedunculate and sessile oaks in response to drainage, fertilization and weeding on acid pseudogley soils" ppt
... broad-leaved species, and results are more contrasting, from clear positive effects (Belgrand and Lévy, 1985) to the absence of effect (Kollist, 1975) and even negative effects in young stands (Holstener-Jørgensen ... early stages of the stands (eg, Bialkiewicz, 1976; Wang et al, 1985; Hauser et al, 1993) as well as in mature stands (Pakhuchii, 1978; Dang and Lieffers, 1989; Trettin and Jones, 1989), however ... treated for centuries as coppice-with-standards stands, in which oaks (Quercus robur L and Q petraea [Matt] Liebl) are the predominant species in the standard trees In many places, the foresters...
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Báo cáo y học: "Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis" doc
... American College of Rheumatology [24], and 12 healthy volunteers (2 men and 10 women) The mean ages ± SEM of RA patients and healthy controls were 52.9 ± 4.8 and 49.3 ± 1.9 years, respectively The ... cells and NK cells are the major source of IFN-γ in PBMC cultures, and IL-12 and IL-18 augment IFN-γ production by these cells Defects in IFN-γ production may result from changes in cell number and/ or ... purchased from Sigma-Aldrich SARL (St Quentin Fallavier, France) Preparation of synovium and cell cultures R40 Peripheral blood samples were obtained from 14 patients (2 men and 12 women) with RA...
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Báo cáo y học: "Vaccination response to tetanus toxoid and 23-valent pneumococcal vaccines following administration of a single dose of abatacept: a randomized, open-label, parallel group study in healthy subjects" pot
... were obtained for subjects of Groups A and B at study days 14 and 28, for Group C subjects at study days 28 and 42, and for Group D subjects at study days 70 and 84 Statistical methods All subjects ... tetanus toxoid and 23-valent pneumococcal vaccines Serum samples were obtained on study day 14 prior to vaccinations and at 14 and 28 days after the vaccinations (study days 28 and 42, respectively) ... serotypes at 14 and 28 days after vaccination are illustrated in Figure 4a and 4b, respectively In general, and as expected, the highest responses were observed for serotypes 14 and The apparent...
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báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx
... ABA and methyl viologen (MV), by extracting total RNA from these plants and performing quantitative real-time PCR (qRT-PCR) Compared to untreated plants, 150 mM NaCl, 250 mM mannitol, μM ABA and ... mannitol and (d) 0.2 μM methyl viologen Relative expression levels were calculated and ACTIN (At3g18780) and 18S rRNA (At3g41768) here used as reference genes (B) GUS expression in AtTSPO-437::GUS and ... OxM1TSPO:eGFP and tspo-1 lines compared to wild type plants, by qPCR 5-day-old seedlings grown under standard conditions and transferred for hours to plates containing 150 mM NaCl (A) DREB2A, (B) RAB18 and...
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báo cáo khoa học: " The Chinese government’s response to drug use and HIV/AIDS: A review of policies and programs" potx
... written in English and published in international peer-reviewed journals between 1990 and 2008, (2) harm reduction intervention studies among drug users in mainland China and (3) related government ... incarceration, voluntary counseling and testing and harm reduction intervention Results Current policies regarding drug use and HIV prevention among drug users In 1990, the Standing Office of National ... professional staff and funding to provide these services, especially at the city or county level Police and medical staff lack the knowledge and skills for delivering harm-reduction and behavioralchange...
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báo cáo khoa học: " Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments" ppt
... England Biolabs, Ipswich, MA, USA) and/ or Bsp HI (Fermentas), purified again and cloned into Nco I digested pCsGFPBT (GenBank: DQ370426) vector with a Gly-Alarich peptide linker between CDSs and ... (AOC) [88] and plant defensin 1.2 (PDF1.2) for JA [50]; ethylene insensitive (EIN2) [89] and ethylene responsive factor (ERF2 and ERF4) [90] for ET; ABA insensitive (ABI5) [91-93] for ABA, and Arabidopsis ... expression in response to two hormones (Table 2) At h, both JA and ET repressed BnWRKY11 and both ET and BAP repressed BnWRKY1, 20 and3 2 (Figure 4C, Table 2) However, none of the genes were observed...
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Báo cáo khoa học: "RAGE: Exacting a toll on the host in response to polymicrobial sepsis and Listeria monocytogenes" pps
... RAGE ligands as well) HMGB-1 may stimulate interferon-γ (IFN-γ)-producing cells and thus mediate macrophage activation Furthermore, HMGB-1 may directly activate macrophages via RAGE and/ or Toll-like ... to splenic macrophages and is internalized; Listeria produces products that activate nuclear factor-kappa B and upregulate innate immune molecules such as CC-chemokine ligand CCL2 [5] Infected ... loss of a single TLR and permit macrophage activation and bacterial killing Unlike the TLRs, RAGE is not likely to be activated directly by microbial products However, RAGE ligands inducibly expressed...
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Báo cáo y học: "Aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic AMp" doc
... fumigatus proteases) and proteins of unknown to date functions (Asp f 2, -4, -7 and -17) Asp f 11, -12 and -22 were induced 5-10 fold under the same conditions Expression of Asp f (peroxiredoxin) and ... complex and clearly lacks the coordinated nature of the responses observed in axenic culture (Figure 4) The protease allergens, Asp f 5, -10 and -13 and -18 were expressed at very low levels and ... containing monocytes and lymphocytes was removed, and transferred to a new sterile tube The cells were washed once with Dulbecco’s Phosphate Buffered Saline (PBS) (Sigma-Aldrich) and centrifuged for...
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REGULATION OF CHOP TRANSLATION IN RESPONSE TO eIF2 PHOSPHORYLATION AND ITS ROLE IN CELL FATE
... valuable time and advice over the years I am grateful to Sheree Wek for her help, and my fellow lab members and friends Souvik Dey, Tom Baird, and Brian Teske for technical help, support, and suggestions ... and ε in mammals, and the yeast counterparts Gcn3p, Gcd7p, Gcd1p, Gcd2p, and Gcd6p, respectively (6, 21, 22) The γ and ε subunits facilitate the catalytic function of eIF2B, while the α, β, and ... first AUG codon, and selection can be enhanced by an optimum sequence context -GCC(A/G-3)CCAAUGG+4-, with the initiation codon in underline and bold and a flanking purine at the -3 and a G at the...
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Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus
... (α, β and ω) IFN genes and proteins found in mice and human, there is only one IFN-γ gene and protein IFN-γ maps to a single locus located on the long arm of chromosome 12 in the human, and chromosome ... Cy3 and Cy5 and run in four technical replicates (2 x Cy3 and x Cy5; figure 6) Independent labelling of each protein sample with the two fluor minimal dyes Cy3 and Cy5 enabled identification and ... B-, and NK cells), proliferation of fibroblasts, chemotaxis of monocytes, neutrophils, and lymphocytes; stimulation of prostaglandin E2, increasing in numbers of peripheral blood neutrophils, and...
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báo cáo khoa học: " Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection" doc
... contributions AB contributed to extracting RNA and making the 454 libraries, curated and analyzed the data, supervised the work SC and YZ, and wrote the paper CS and YZ contributed to the bioinformatics ... prepared RNA, cDNA and 454 libraries, and helped prepare the first draft of the manuscript KB and WAP contributed to the RNA preparation and discussion of disease response gene candidates NW manages ... healthy stem and canker tissues from American and Chinese chestnut were compared (Fig and Fig 3) As predicted, we observed that the fraction of genes involved in response to stress, biotic and abiotic...
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Báo cáo y học: " γδ T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-α and IFN-γ-producers in response to Pseudomonas aeruginosa" pptx
... and days of Figure PBMC culture with cytosolic extract in αβ T lymphocytes (top) and Percentage of CD25-expressing cells in αβ T lymphocytes (top) and γδ T lymphocytes (bottom), after 0, 4, and ... was added to PBMC cultures at 50 µg/ml CD25 and CD45RO expression by αβ and γδ T cells was evaluated by three-color flow cytometry before and after 4, and days of culture We chose these days of ... Serotec (Oxford, England) The cells were then washed and resuspended with 100 µl of 1% paraformaldehide Samples were acquired and analysed using CellQuest software (BD) Ten-thousand T lymphocytes...
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Microarray analysis of host transcription in response to infection of arabidopsis thaliana protoplasts by coat protein mutants of TCV and HCRSV
... encouragements and help have made my work and study easier and more enjoyable I would also acknowledge the financial support of National University of Singapore and emotional support of my family and friends ... Immunization and tests of sensitivity and specificity…….31 3.3 Results and discussion………………………………………… 31 3.3.1 Expression of TCV and HCRSV CPs…………………… 31 3.3.2 Test of antibody against TCV CP and HCRSV ... agarose gel, and target band was excised from the gel under long wavelength UV light The DNA was purified using QIAquick Gel Extraction Kit (QIAGEN, Netherlands) Weight of gel was measured and volumes...
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Báo cáo y học: " Facilitators and obstacles in pre-hospital medical response to earthquakes: a qualitative study"
... words and phrases, incidents and facts in the text were noted Primary codes were extracted The codes and data were compared for similarities and differences Categories and sub-categories were developed ... in the conception and design of study and took an active part in the data analysis and results interpretation GO participated to the study conception and design, writing-up and finalization of ... analyze and interpret the data and to write the manuscript LK participated to the study design, analysis and results interpretation and writing-up of the manuscript All authors read and approved...
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Báo cáo y học: " Factors affecting the long-term response to tacrolimus in renal transplant patients: Pharmacokinetic and pharmacogenetic approac"
... 125- and 20- bp bands (lanes 1, 2, 3, 6, and 9) and CYP3A5*1/*3 genotype gives 168-, 148-, 125- and 20- bp (lanes 4, 5, and 10) CYP3A5*3/*3 genotype is not seen in this picture The 20- bp band ... CYP3A5*1/*1 genotype gives 148-, 125- and 20- bp bands, CYP3A5*3/*3 genotype gives 168- and 125-bp bands and CYP3A5*1/*3 genotype gives 168-, 148-, 125- and 20- bp [19] Statistical analysis Tacrolimus ... England Biolabs) 25 μL PCR product was incubated for hours at 37οC with 2μl SspI 5Units/μl and 3μl 10xBuffer A 3.5% agarose/Tris-borate EDTA gel with ethidium bromide staining was used and bands...
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Báo cáo y học: "Weight loss, leukopenia and thrombocytopenia associated with sustained virologic response to Hepatitis C treatmen"
... schizophrenia (6%) and bipolar disorder (3%) Underlying medical problems included hypertension (15%) and diabetes mellitus (DM) (14%) Demographics, clinical and laboratory data of patients (percent and means) ... which has a limit of detection of 28 IU/mL Body weight measurement and laboratory data were collected and compared at the beginning and at the end of therapy The Knodell scoring system for liver ... laboratory data and liver biopsy results of all patients are shown in Table The major risk factors for acquiring HCV infection were related to injection drug use and tattooing Mild and moderate...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose