and ccr2 deficient mice develop ahr and produce mucus in response to aspergillus antigen
Báo cáo y học: "Aspergillus antigen induces robust Th2 cytokine production, inflammation, airway hyperreactivity and fibrosis in the absence of MCP-1 or CCR2" pptx
... compared to PBS controls MCP-1- and CCR2- deficient mice develop AHR and produce mucus in response to Aspergillus antigen To determine airway reactivity to acetylcholine in mice exposed to Aspergillus ... -/- Ccr2 - /Aspergillus Figure antigen induced AHR in wild-type, Mcp-1-/- and Ccr2- /- mice Aspergillus Aspergillus antigen induced AHR in wild-type, Mcp-1- /and Ccr2- /- mice Airway reactivity in response ... cells These findings indicate that Aspergillus antigen induces AHR and mucus production even in the absence of MCP-1 or CCR2 Th2 cytokine and IgE production is similar in Aspergillus antigen- treated...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
... findings stand in contrast to a subsequent analysis of singlegene knockout data indicating that E coli can only survive if at least L1-P-EtAmine and a lipid A form lacking the myristoyl side chain ... metabolites produced by both enzymes are converted into either glycerol and cardiolipin, or L1-P-EtAmine Eliminating either of the two enzymes reduces the possible pathways by 50% (95 EFMs remain), and ... (Table 6) In the case of elongation of fatty acids, we decided to split the cycles into several parts and combined some of the reactions in these parts We combined most of the enzymes operating on...
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Báo cáo y học: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels" doc
... step is to eliminate proteins within the section which could otherwise interfere with binding of the probe to the target DNA b The aim of this step is to eliminate all RNA molecules including viral ... into the nucleus points to a Flap-independent nuclear import mechanism that likely accounts for transduction levels obtained with Flap- vectors, a current area of investigation in our laboratory ... vivo in all tissue- and cell-types examined [5-14], thus making the DNA Flap an essential and widely-used component of lentiviral gene therapy vectors mizing the detection of vector DNA within...
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Tài liệu CUSTOMS DECLARATION AND LIQUIDATION SOFTWARCUSTOMS DECLARATION AND LIQUIDATION SOFTWARE USAGE GUIDLINE FOR BUSINESS - PRODUCTION TYPE ECUS_KD 1.2 docx
... declaration sheet Print out the receiver number and bring it to the Customs Office to customs procedures with a click on button in on the window of inputing declaration sheet data as followings: 24 The ... error 36 Print out the receiver number and bring it to the Customs Office to customs procedures with a click on button in on the window of inputing declaration sheet data as followings: The ... (Import goods, Export goods) in standard list automatically according to the goods code you have input in Incase the goods code you input does not exist in the standard list, the program will...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
... protease inhibitors, which include Kunitz-type inhibitors and serpins [2] Hepatocyte growth factor activator inhibitor type (HAI-1), a Kunitz-type serine protease inhibitor, is implicated in the inhibition ... extracellular matrix in the tissues producing it In response to tissue injury, such as hepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively in the injured tissue ... subfamily, forms stable inhibitory complexes with plasminogen activator inhibitor-1 and protein C inhibitor [21] Thus, these serpins might be endogenous inhibitors of TMPRSS11A, HAT-like and HAT-like The...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... the interaction with the PDZ proteins: Mint-3, AMPA binding protein and glutamate receptor interacting protein (GRIP) It would therefore be interesting to determine whether this motif is also involved ... UK) were maintained in DMEM containing 10% (v/v) fetal bovine serum (Hyclone Laboratories Inc., UT, USA), mm l-glutamine, 100 UÆmL)1 penicillin and 100 lgÆmL)1 streptomycin at 37 °C in 5% CO2 ... subsequent to its fusion to the GAL4 DNA binding domain We therefore cannot rule out an interaction between MT1-MMP ICD and the GRASP55 PDZ1 domain GRASP55 binds to furin, PC5/6B and PC7 intracellular...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... Recombinant wild-type chitinase A was obtained by cloning the chitinase A gene, lacking the C-terminal proteolytic fragment, into the pQE60 expression vector and expressing the protein in E coli M15 cells ... substrate distortion, leading to glycosidic bond cleavage, to yield an oxazolinium intermediate and to retention of anomeric configuration in the products Detailed characterization and kinetic analyses ... running buffer was 20 mm Tris ⁄ HCl, pH 8.0, containing 150 mm NaCl A flow rate of 250 lLÆmin)1 was applied and fractions of 500 lL were collected Chitinase-containing fractions were combined and...
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Báo cáo khoa họcRe-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme docx
... often puried in research laboratories prior to use [30,31] in order to avoid misleading kinetic anomalies, but this routine has largely been abandoned in recent years because the purity and stability ... [24,25], were aimed initially at facilitating productive binding of the phosphorylated coenzyme by enlarging the potential binding pocket and removing the negative charge, likely to repel the 2Â-phosphate ... discrimination is never total, and the actual factor varies widely from enzyme to enzyme Particularly interesting, however, are those enzyme families that include members showing little discrimination,...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... stacking prevails in all the steps, both in DDwt and DDmut C7 and A24 involved in A .C mismatch in DDwt and the equivalent T7 and A24 in DDmut are coloured red Trajectories corresponding to every ... of minor and major grooves are around 9.5 A ˚ in both PDwt and PDmut and 25 A Sugar puckers in DNA strands favour the C2¢ endo conformation in both PDwt and PDmut Interestingly, C7 involved in ... entropic gain due to interrupted stacking, fluctuating nature of the hydrogen bond and water organization in the vicinity of the mismatch might be the contributing factors for the increase in free...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... tagged PSI-G into PSI Whilst it is evident that both C-terminally and looptagged PSI-G can insert into the thylakoid in vivo (Figs and 5), it is possible that the tags hinder assembly into PSI complexes ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... glycine, glycine-ester and D-pipecolic acid was only observed using GO Interestingly, D-proline is the only amino acid in which the a-amino group is involved in a covalent bond with the side chain ... subtilis GO in E coli (up to 3.9% of total soluble protein) using two different expression systems In particular, we produced a recombinant GO containing a polyhistidine tag at the N-terminus, that ... was inserted into the EcoRI site of the MCS of pT7-DBam/Hind plasmid [6] The original ATG starting codon is shown in bold and the ATG codon corresponding to the Met1 of the wild-type GO in italic...
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Báo cáo khoa học: Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme docx
... first the binding of RXP407 to the N-domain at low inhibitor concentration, followed at higher concentrations by inhibitor binding to the C-domain The location of the in ection points in this profile ... N-domain, only IC50 values for inhibitor binding to the N-domain can be obtained from the inhibition profile Thus, affinity of the inhibitor for the mouse ACE C-domain cannot be determined using ... C-domain varies according to the ACE species For rat and human enzymes, estimation of IC50 values for the binding of the inhibitor to the N-domain and C-domain can be deduced from the inhibition...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... CcStP appears to differ subtly from maltodextrin and glycogen phosphorylase in how it copes with constraining the cofactor phosphate group into a configuration that is believed to promote catalysis ... given ligand therefore provide a characteristic fingerprint of nonpolar ligand interactions within the binding pocket of the protein [21–27] Because hydrogen bonds and other electrostatic interactions ... an overwhelmingly local disruption of binding interactions caused by removing the two functional groups individually or together Analysis of kinetic consequences in the H334G mutant and chemical...
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Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc
... with bovine serum albumin standards Enzyme assays and protein determinations Glutaminase assay CTPS activity was determined at 37 C using a continuous spectrophotometric assay by following the ... resin per unit of thrombin following the Novagen protocol [35] Cleaved CTPS, free of biotinylated thrombin, was then dialysed against HEPES buffer (70 mM, pH 8.0) containing EGTA (0.5 mM) and ... N-terminal His6-tag The BL21(DE3) cells were grown in LuriaBertani medium at 37 C, induced using isopropyl thio-b-D-galactoside according to the Novagen expression protocol [35], and lysed using...
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Báo cáo khoa học: Regulatory feedback loop between NF-jB and MCP-1-induced protein 1 RNase pdf
... construct To verify the ability of sites N1–N4 to bind activated NF-jB complexes, EMSA was performed Radiolabelled probes corresponding to the original sequences of nominated NF-jB-binding sites and to ... places the MCPIP in the negative regulatory loop of the cell’s response to the in ammatory state NF-jB is a transcription factor known to play a central and crucial role in regulating such complex ... mode and stage of MCPIP action still need to be determined Altogether, observations concerning the mutual regulatory effect between MCPIP and NF-jB, its implication in the in ammatory state and...
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Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt
... to be related to the thermal unfolding of the protein structure [11] This unfolding study was based mainly on monitoring tryptophan fluorescence There are altogether nine tryptophan residues in ... maximum and quantum yield are sensitive to the microenvironment of tryptophan side-chains in a protein, and both parameters can be used to monitor structural changes and the unfolding of proteins In ... dependent, and, depending on the enzyme, the pH stability and activity curves can in theory overlap totally, partially or not at all When thinking of applying an enzyme at certain pH and temperature...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... 15000; C and D: 90000 In this study, we investigated the interactions between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine...
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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
... expressed in the brain in response to ischemia and hypoxia [1-4] Hypoxia or ischemia stimulates the expression of inflammatory cytokines (IL-1β, TNF-α), chemokines (IL-8, MCP1/CCL2) and adhesion ... adhesion molecules (ICAM-1) in the brain and in cultured astrocytes and brain endothelial cells [510] These inflammatory mediators play a critical role not only in the initiation and propagation of ischemica/ ... poly-lysine The cells were cultured in an atmosphere of 5% CO2/95% air at 37°C in D-MEM containing 4.5 g/L glucose, mM glutamine, 25 μg/ml gentamycin (Invitrogen, Burlington, ON), and 10% fetal bovine...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... 15000; C and D: 90000 In this study, we investigated the interactions between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine...
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báo cáo hóa học:" Frequency of Drug-Resistant Variants of HIV-1 Coexistent With Wild-Type in Treatment-Naive Patients of India" pdf
... North India who came to a voluntary counseling and testing center in the Department of Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India, for testing and ... HIV-1 in primary infection leading to super infection AIDS 2004, 18:1653-1660 Abstract Palmiter RD, Chen HY, Messing A, Brinster RL: SV 40 enhancer and large T antigen are instrumental in development ... to zidovudine The point mutation K70R causes low-level (4- to 5-fold) resistance to zidovudine and is usually the first drug resistance mutation to develop in patients receiving zidovudine monotherapy.[14,15]...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008