analysis manual curation and pathologic incremental updates
Báo cáo y học: "Nucleosome deposition and DNA methylation at coding region boundarie" doc
... oftheand highergene expression and for and region in yeastfileofin thestart codon and levelcodons codinghigh DNA inside genes withsurroundingto differential codon, Pol II Illustrative data UTR and ... carried out the analysis, and wrote the manuscript JBB carried out the purification and sequencing of methylated DNA JL processed the raw sequencing data and participated in data analysis TYK participated ... transformed (base e) Genetic and epigenetic codes near the start codon and stop codon For quantitative analysis of genetic or epigenetic codes near coding start and end sites, we used the DNA...
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Characterization of high efficiency pseudo bilayer organic solar cells and
... invaluable and is much appreciated My special thanks to the staff, colleagues and friends at SERIS and Chemical and Biomolecular Engineering Department with whom I have had the privilege to read and ... P3HT and PCBM were used to study the effect of continued exposure of moisture and oxygen and its effects on the device performance It was found out that the effect of oxygen was permanent and ... annealed sample was found to be 4.4% and at 30 minutes, 40 minutes, 50minutes and 60 minutes (annealing at 140ºc) the efficiency was found to be 4.3%, 4.6%, 5.6% and 5.2% respectively (d) Efficiency...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... blood examination and liver and renal functions, no manifestations of acute and chronic toxic reactions and graft versus host disease, no local swelling at injection sites, and no lower limb ... of acute and chronic toxic reactions and graft versus host disease were observed during and after MSC transplantation Meanwhile, the body temperature, routine blood parameters and liver and renal ... from each section were randomly selected and GFP positive cells were counted at a magnification of 200 Data were expressed as means ± standard deviation (SD) Statistical analysis was performed...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... parameters, and wrote the manuscript; PAD, CMG, RCG, and TSG designed, performed or analyzed in vitro ultrasound experiments, MGO, PAD, GJM and DCS analyzed data and directed Pilot Studies and Study ... necropsies and supervised all animal experiments performed at ILS; EJRS and KGH treated animals, performed necropsies and analyzed sperm All authors participated in experimental design and read and ... [26,27], and in the United States there are about 247 thousand collisions each year between deer Page 13 of 15 and automobiles that damage approximately billion dollars in property and kill approximately...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
... 25 lm and n ¼ at ALI after weeks] Epidermal thicknesses measured in ALI cultures under sham and GSM-900 exposure were, respectively: 41.5 ± 8.7 and 37.9 ± 6.8 lm after weeks, 56.6 ± 9.9 and 45.0 ... Hsc70 On the one hand, the Hsc70 expression pattern was different and, on the other hand, RFR exposure had no effect on Hsc70 expression in NHDFc It is, however, not clear why NHDFe and NHDFc react ... absorption in ear and eye region of children and adults at 900, 1800 and 2450 MHz Phys Med Biol 50, 4355–4369 ` ´ ´ Boisseau AM, Donatien P, Surleve-Bazeille JE, Amedee ´ J, Harmand MF, Bezian JH,...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... delayed-extraction and reflectron systems and a N2 laser (337 nm) operating with 3-ns pulse width and 107)108 WÆcm)2 irradiance at the surface of 0.2 mm2 spots In addition to the positive mode standard procedure ... individual clones were isolated at random, exposed to 0.05 lM MTX, expanded, and checked for the presence of human HCII mRNA by RT-PCR Positive cell lines were picked and after several rounds of selection ... human plasma J Biol Chem 257, 2162– 2169 Sandset, P.M & Andersson, T.R (1989) Coagulation inhibitor levels in pneumonia and stroke: changes due to consumption and acute phase reaction J Intern Med...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... is to provide candid and critical comments that will assist the institution in making its published report as sound as possible and to ensure that the report meets institutional standards for objectivity, ... the committee’s findings and recommendations It is based on careful consideration of information presented at the workshop and on data and opinions found in the scientific and other scholarly literature ... the moral and legal status of the early embryo The controversy has encouraged provocative and conflicting claims both inside and outside the scientific community about the biology and biomedical...
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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc
... unbound antibody and toxin, and the temperature was changed to 37 °C in order to restore transport and thereby allow the endocytosis of GD3 and GM1 for different times Confocal microscopy analysis revealed ... labelled from either surface with the antibody R24 and CTx, specific for GD3 and GM1, respectively The binding of R24 antibody and CTx in both apical and basolateral domains was evaluated by western ... the subcellular distribution and intracellular transport of GD3 and GM1, representative complex gangliosides from series ‘a’ and ‘b’, respectively Using biochemical and fluorescent confocal microscopy,...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... sPLA2-IID (mIID) and its mutants, KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2, respectively (B) Expression of the wild-type (WT) and two mutants ... regulatory mechanisms for gene expression, and perhaps functions, of sPLA2-IID and those of sPLA2-IIA and -V are not entirely identical and are even cell- and tissue-specific Searching the nucleic ... physiological and pathological states and in different cells and tissues Ó FEBS 2002 Analyses of group IID phospholipase A2 (Eur J Biochem 269) 2705 ACKNOWLEDGEMENTS We thank G Lambeau (CNRS-UPR) and...
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Báo cáo khoa học: LRRK2 in Parkinson’s disease: function in cells and neurodegeneration doc
... neighbors b-RAF and mixed-lineage kinase (MLK)3 require self-interaction and dimerization for proper regulation and activation [20,21] Similarly, LRRK2 forms structures consistent with dimers and oligomers ... biochemical separations and immunocytochemistry [12–15] A portion of LRRK2 protein is soluble and readily resolved, whereas another portion resolves only with strong detergents and reduced conditions ... allows kinase activity, autophosphorylation and stabilization of a P J Webber and A B West kinase-active homodimer (Fig 1) Competing phosphatase activity and loss of GTP, as well as the subsequent...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... in b-cell dysfunction and apoptosis in rodent models of diabetes [37–40], and changes in mitochondrial function, including increased ROS and UCP2 expression, lower ATP and a decreased ATP ⁄ ADP ... two bands in cells incubated with recombinant PHB, the top band corresponding to His-tagged PHB (Fig 5A) These data indicate that exogenous PHB enters the cells PHB is expressed in b-cells and ... described in Materials and methods The results are expressed as the mean ± SEM percentage of G2 for (A) and (B); as the mean ± SEM arbitrary units relative to actin for (C); and as the mean ± SEM...
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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... steel sample plate and dried in air prior to analysis External calibration was performed with standards using the averaged m/z values of 1297.51, 2094.46, 2466.72, 3660.19 and 5734.59 Synthetic ... 2H-enriched-TFE, H2O and 2H2O was adjusted to pH 5.5 The standard COSY, TOCSY as well as 100 and 200 ms mixing time NOESY spectra were accumulated, processed by NMRPIPE [21] and analyzed by X-EASY ... Kozlowska (IBB) in HPLC and of M JerkaDziadosz (Nencki Institute, Warsaw) in immunofluorescence technique is acknowledged We are grateful to A Wyslouch (IBB) and to M Jaquinod and J.-P Andreini (IBS) for...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... 271) 4673 Fig Analysis of FAAH promoter by transient expression (A) Proximal upstream region of human FAAH gene Left and right handed arrows denote SP1 sites in the – and + strands, respectively ... were validated by size determination and sequencing, as described previously [18] Analysis of N-acyltransferase, phospholipase D, anandamide transporter and leptin receptor binding An N-acyltransferase ... competition assays [18] Ó FEBS 2004 Western blot analysis of protein phosphorylation and nuclear levels of Ikaros For the analysis of total STAT3 and of the corresponding phosphorylated (activated)...
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Neural Stem Cells and Therapy Edited by Tao Sun potx
... Carvalho and Inês M Araújo Chapter 19 Immune System Modulation of Germinal and Parenchymal Neural Progenitor Cells in Physiological and Pathological Conditions 413 Chiara Rolando, Enrica Boda and ... positive to BrdU and NF200 (D and E) -Tubulin-III (Tuj1, brown) and BrdU (blue) immunohistochemistry on the normal (D) and denervated (E) hippocampus Arrow showed the -Tubulin-III and BrdU positive ... neurological diseases and injuries The publication of this book is timely It contains the characterization of embryonic and adult neural stem cells in both invertebrates and vertebrates, and highlights...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... 94 °C and 30 s at 68 °C PCR products were cloned in pEGFPn3 (Clontech) and sequenced on both strands Tissue extraction Mouse spinal cord tissue Animals were handled in accordance with standard ... NPFF-related peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanospray ion trap tandem mass spectrometry ... NPFF-IR molecular forms in SH-SY5Y (peaks and 3) and COS-7 transfected cells (peaks and 3) was provided using MS/MS analyses Identification of SQA-NPFF and hNPAF by capillary HPLC/nanospray MS/MS...
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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx
... J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germs layers Mol Med 6, 88 - 95 Itkin - ansari, P., et al (2001); PDX - and ... la Tour, D., et al (2001); B - cell differentiation from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive ... khảo Amit et al (2000); Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture Dev Biol 227, 271 - 278 Beltrami, A...
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cells and robots - milutinovic and lima
... world And huge thanks to my wife Dragana Milutinovi´ for her understanding, patience, emotional support and the c book proofreading This research was supported by the Institute for System and Robotics, ... explain based on the analogy between the robot and the cell Let us imagine that someone provides us a brand new mobile robot and asks us to understand in full detail how it works without even telling ... and negative selection of immature T-cells in the thymus [77], and also in the survival, activation, differentiation, and cell cycle progression of mature circulating T-cells [63, 81] Understanding...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells coexpressed the HCC cells-derived WT1 antigens and ... levels of WT1 and HLA-ABC (HLA-A2+/A24-) and low levels of CEA but not HLA-DR, costimulatory molecules (CD80 and CD86), and maturation marker, CD83 (Figure 5A) Before the vaccination and one month ... contributions SK, SH, and EH conceived of the study, participated in its design, coordination, and preparation of this manuscript SK, MM, AT, EN, and, YN prepared reagents and PBMCs SH, HK, and YS prepared...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... through DNA analysis (Tepnel Lifecodes Luminex, UK) of Allergy and Immunity, Emery University, USA Tetramers were conjugated to Phycoerythrin (PE) and shipped at a concentration of mg/ml and the ... American Type Culture Collection (ATCC) and maintained in Iscove's Modified Dulbecco's Medium supplemented with glutamine, and penicillin and streptomycin (100 IU/ml and 100 μg/ml, respectively, Sigma-Aldrich, ... prelabelled) with 100,000 PBMCs and incubated for h Cells were stained with FITC-conjugated Annexin V and ToPro3 (Sigma Aldrich, UK) and cells that were PKH26+, annexin high and ToPro3 high were regarded...
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Báo cáo hóa học: "Lasers, stem cells, and COPD" docx
... infarct size, 5- and 6.3-fold significant increase in cell density that positively immunoreacted to BrdU and c-kit, respectively, and 1.4- and 2-fold higher level of angiogenesis and vascular endothelial ... trial 660 stroke patients were recruited and randomized: 331 received LLL and 327 received sham No prespecified test achieved significance, but a post hoc analysis of patients with a baseline National ... ulcers, and various degenerative conditions, despite no clear mechanistic understanding [69-71] In vitro studies have demonstrated that ozone is a potent oxidant and inducer of cell apoptosis and...
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