amplification cloning and sequence analysis of the complete env genes of a wide variety of srv 2 isolates
Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
... Y26 Y27 TCAATAGATCTCCCGTGCTCAGATCAACAGG ATACTAAGCTTTGCAGCAGTGGGGAAGGAGA TCTACCTCGAGTTCCAACACGGAGTTTCGCT TCTACCTCGAGGCGATTCTTCTGCCTCAGCG TTTACCTCGAGCAGGACATCTGACCATTCTC ACTACCTCGAGGTGCCGTGGTTCATGCCTGT ... 5¢-CTTGCAGTGAGCTGAGATCATAACAAT GACTCCAGCCTGGGCAACAGAG-3¢; M-D sense primer, 5¢-TGGGAATGTGAGGCTGGGGGTCTTCTTT CGGAACGCTGCTTCTCAAGGG-3¢; and M-D antisense primer, 5¢-CCCTTGAGAAGCAGCGTTCCGAAAGA AGACCCCCAGCCTCACATTCCCA-3¢ ... TCTACCTCGAGGGAACGCTGCTTCTCAAGGG TCTACCTCGAGAGGGCCTCGGGCCCTTGTCA TCTACCTCGAGTTGTCAGTGGGCACAGGAAC TCTACCTCGAGACAGGAACCCCCGCACCCCC ATCATGGGCCCCGTTCCACCCTGCTCCCCCA TTCATGGGCCCGCCTCACATTCCCACCTGCA ATCATGGGCCCGGTCAGATGTCGCGCCTTTC...
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Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon)
... the Fig 1; a Histidine kinase-like ATPases (HATPase-c) domain from aa33 to aa187; N-glycosylation sites: NSSDaa44–aa47, NKTKaa279– aa2 82 , NISRaa385–aa388, NTSKaa 428 –aa431; two coiled coil: 21 2 ... sequence ATTTA which known to decrease both the stability and translation efficiency of an mRNA [28 , 29 ] There are two polyadenylation signal sequences, one is AATAAA, same as most animals, the other ... Bemisia tabaci (AAZ17403), Ceratitis capitata (CAJ28987), Salmo salar (AAD3 027 5), Chiromantes haematocheir (AY 528 900), Locusta migratoria (AY445913), Gallus gallus (P11501), Mus musculus (BC094 024 ),...
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Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx
... Kakizuka, A (20 02) Identifica- Analysis of ataxins and (Eur J Biochem 27 1) 3169 122 123 124 125 126 127 128 129 130 131 1 32 133 134 135 136 137 138 tion of ter94, Drosophila VCP, as a modulator of ... [70] (also termed LALAL motifs and PUBs [71], amino acid 22 3 24 0 and 24 3 26 0) and the polyQ region (amino acid 29 6–317) (Fig 4) [ 72] A slightly shorter alternative splice variant of ataxin-3 ... UFD 2a/ E4B, which is a U box homologue of yeast Ufd2 [ 128 ], and interacts with and regulates the degradation of the proteasome-associated ataxin-3, forming a trimeric complex of ataxin-3, VCP, and...
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Báo cáo y học: " Complete coding sequence characterization and comparative analysis of the putati" docx
... variation (nt%) 27 26 25 26 19 21 32 26 30 25 29 Amino acid variation (aa%) 14 31 25 23 16 19 26 12 23 16 24 NS variation (aa) NS variation (%) 19 80 58 56 42 63 44 23 41 19 43 34 19 20 36 29 ... participated in the sequence alignment and drafted the manuscript SP and KS participated in the sequence alignment PL and YP participated in the design of the study and performed the data statistical ... Williamson C, Posada D: RDP2: recombination detection and analysis from sequence alignments Bioinformatics 20 05, 21 (2) :26 0 -26 2 Yozwiak NL, Skewes-Cox P, Gordon A, Saborio S, Kuan G, Balmaseda A, ...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... forward SP6-reverse GACTGTTGCTCATTTGTGGA GAGGCTAGATACTGCTCGATGT TGATGATTTGGAACCATTATTGAA CACCTTTTTCCTTCATCTTTTCAT ACTACCTCATGAAGATCCTG TTGCTGATCCACATCTGCTG TAATACGACTCACTATAGGG ATTTAGGTGACACTATAGAA ... Overall Mature CAD 624 46 CAD67773 NP_000563 AAC64705 CAA43090 A3 4853 43 .2 37.6 28 .0 28 .5 25 .2 25 .2 45.7 39.3 26 .6 26 .6 25 .1 25 .1 CAA24863 27 .4 AAB26148 28 .0 CAA24863 27 .0 26 .9 26 .6 25 .7 AAG16755 ... isolated and characterized a carp cDNA sequence that is homologous to the DNA sequence of mammalian IL-10 Carp IL-10 is 1096 bp in length and encodes a 180 amino acid protein similar to that of...
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Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx
... mediator, platelet activating factor [23 ,24 ] The molecular biological analysis of N brasiliensis genes and gene products is at a very early stage Secreted and intracellular globins have been characterized ... taxa to the Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence analysis [48] The set of ... projects have added Litomosoides sigmodontis [43], Toxocara canis [44] and many other related species to the available database of parasitic nematode sequences [36] In designing a study on N brasiliensis,...
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Báo cáo y học: "Sequence analysis of the Epstein-Barr virus (EBV) BRLF1 gene in nasopharyngeal and gastric carcinomas" docx
... BRLF1 -A1 GGTGCAATGTTTAGTGAGTTAC 103187 - 10 320 8 BRLF1 -A2 ACCAAGAGAGCGATGAGAGA 104 021 - 1040 02 BRLF1 -A3 GGAGGCAGTTTTCAGAAGTGT 103345 - 103365 BRLF1-B1 TTTGGCTGACACACCTCTCG 103847 - 103866 BRLF1-B2 ... of 57 NPCs, and all the TWs) The rest mutations in this domain affected the weaker accessory activating subregions (AA 3 52 to 410 and 450 to 500) Variation analysis of CTL epitope sequences among ... was affected by an S®N change at position 14 of the epitope, and existed in the majority of the specimens (21 EBVaGCs, 50 NPCs, and 28 TWs) Mutation A E at position three of the NAA epitope was...
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Báo cáo khoa học: " Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine" pdf
... AGGCCAGGATGTCAACACTGGCAC ATGTATGGATAGCCCTCAGATTG GTCCACATCAACGGCCGCCGGCTCG AGCCAACAGACACTCCTGTGTTCC CATGCCAGACCCTGATATTATCACC ACCTACACCAATGTCACCTGGAC GTGCCACACCTACTATGACCACAG TCAAGCCTCCAAACCAAGCC ... ATTGACGTGACAGCCCCCAC TGTGGTTCTTGACTGGTGAG TGGTGGAGGCCTGTTCAGAGC CCAAGTTGTGGGCTGTCAACAC CAGAGTCCTCCTGGTGGACATTC ATTACCAAGCGCAACGCTAGGC CATGTGGCCAACATGTGTG TGATTTGGTCAAGGTAGCC CCTTCTACAACACCAAATGATTGCC ... TCAAGCCTCCAAACCAAGCC TGGCGGTCCATAAATGAGGTG TATGCAGCTTTGGCAATTCCC TTGATCTTTAGCAACTGTATCTG TTGGATTGCAGGAGCAATGCAGG TGTAAGGTTCGGTACGCGTAACC TCAATTGGCTGGGTCACGTGAAG CAAACACCTTTGGTCCACCAAGG 1-14 645-667...
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Báo cáo khoa học: " Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination" pot
... characteristics of vaccines used in China and efficacy of vaccination against heterologous strains from China Avian Pathol 20 06, 35:394-399 20 21 22 23 24 25 26 27 28 29 30 31 Mondal SP, Cardona ... 99, AY3 920 51; DE0 72, AY3 920 54;GA98, AY3 920 53; Gray, AY3 920 56; (b) PLpro: Conn, AY3 920 59; Florida, AY3 920 60; CU-T2, AY561734; Ark 99, AY3 920 61; DE0 72, AY3 920 64; GA98, AY3 920 63; Gray, AY3 920 66 ... speculate that GA98 may be a derivative of the Ark DPI strain Analysis of the structural region of Ark DPI clearly demonstrates that it is a chimera of three strains The S1 gene of Ark DPI is probably...
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Comparing the cultural and linguistic analysis of the English word “meal” and words relating to it in contrast with Vietnamese equivalents.
... traditional breakfast as well as conditional breakfast They often have cereal or porridge, egg and bacon followed by toast and marmalade and coffee and tea or they may just have a toast and marmalade ... and jam and cake with a pot of tea 3.1.3 .2 Afternoon tea The tradition of afternoon tea goes many years to the late 1700s In England at that time, there were two main daily meals, breakfast and ... cup of tea in the morning and a nice cup of tea at night And at half past seven, their idea of heaven is a nice cup of tea They like a nice cup of tea with their dinner and a nice cup of tea with...
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Tài liệu Static and Dynamic Analysis of the Internet’s Susceptibility to Faults and Attacks docx
... defined as the average diameter, K, and DIK of the original networks and df , Kf and DIKf denote the diameter, K, and DIK after 10% of the nodes are removed Results are the average of ten runs (a) and ... expect that failures are either all faults or all attacks One may expect that failures are a mixture of attacks and faults, e.g., only a small fraction of failures are attacks while most failures ... measured the average diameter We also generated the BA model and the GE model and measured their average diameters While the number of nodes in the Internet increased, the average diameter actually...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... Thr3-NH/GalNAc-NH GalNAc-NH/GalNAc-H3 2. 35 2. 35 3.03 3.17 2. 48 2. 33 2. 99 2. 83 2. 56 2. 46 2. 52 2.44 2. 99 2. 97 3.13 2. 65 2. 60 2. 60 3.35 3.51 2. 74 2. 58 3.30 3. 12 2.83 2. 72 2.78 2. 69 3.31 3 .28 3.46 2. 93 ... minimize temperature and magnet instability artefacts The so called on resonance irradiation of the protein was performed at a chemical shift of )2 ppm Off resonance irradiation was applied at 40 p.p.m., ... presaturation of the protein was achieved by a train of 40 Gaussian shaped pulses of 50 ms length, each separated by a ms delay, leading to a total saturation time of 2. 04 s The pulse scheme is as...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... accession No BAA094 62) , A thaliana (accession no BAA078 42) , Ipomoea batatas (accession no BAA 022 86), Triticum aestivum (accession No P93594), Zea mays (accession no P55005) and Hordeum vulgare (accession ... different pH values in a range between and 12, and incubated for h at 25 8C Then 0.1 vol of a solution of 0.5 M sodium acetate (pH 5.0) was added and the activity of the enzyme was measured by the dinitrosalicylic ... 7.4) The main peak eluting with an apparent molecular mass around 20 0 kDa was collected, dialysed against appropriate buffers and stored in small aliquots at 22 0 8C until use Analysis by SDS/PAGE...
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..GENE CLONING AND DNA ANALYSIS..GENE CLONING AND DNA ANALYSISAn IntroductionT.A. BROWNFaculty of Life Sciences University of Manchester ManchesterSixth EditionA John Wiley & Sons, Ltd., Publication. pdf
... DNA Analysis in Medicine 24 5 Gene Cloning and DNA Analysis in Agriculture 26 4 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 28 2 Glossary 29 8 Index 3 12 Companion website available ... DNA profiling 28 6 16 .2. 1 Related individuals have similar DNA profiles 28 6 16 .2. 2 DNA profiling and the remains of the Romanovs 28 6 STR analysis of the Romanov bones 28 6 Mitochondrial DNA was ... especially for this purpose Ultraviolet absorbance can also be used to check the purity of a DNA preparation With a pure sample of DNA, the ratio of the absorbances at 26 0 and 28 0 nm (A 26 0 /A 28 0)...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... Q CAG T ACC Q CAA K AAG P CCG K AAG S TCC H CAC W TGG M ATG ACG C TGC G GGT E GAG T ACC E GAA A GCC L CTC K AAG G GGC Y TAC K AAG I ATC B Cyn d 24 CGT I ATC G GGA A GCG R AGG G GGA I ATA T ACG ... GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG L CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC ... STQLPSDEPLNGLNDKAIQDILNEHNMFRAKEHVPPLTWNTTLA 44 Hordeum MQTPKLVILLALAMSAAMVNLSQAQNSP YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... in the pH 4 .2 8.0 range, and a marked increase in Km at alkaline pH (Table 2, Fig 3) The D215N and D140N mutants displayed an acidic shift in the pH-activity profiles, whereas the Y214F and the ... The pKa of Asp140 is much lower than that of Asp1 42 and Glu144 in all situations where all three residues are present and the one proton shared by Asp140 and Asp1 42 appears to remain on Asp1 42 when ... bond ˚ and Asp140 is 10.7 A Mutational effects The residues mutated in this study are shown in Fig Asp140, Asp1 42, Glu144 and Asp215 were mutated individually to asparagine and alanine, Tyr10 and...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... GAATCATTATGAAGCGGTGAGAGCTAATTTTGAAGG TGCTCGTACGCTGCAGGTCGAC TATTTACAAATTTACCTATACGCTCTGAGTTGATATTAC ATCGATGAATTCGAGCTCG GCTAATAACAACTAATATCACTAACAGAAGACCATTAC CCCGTACGCTGCAGGTCGAC GGAGGATTTATCAAAAGCTACTTCGAAAGCTAGGAACG ... CCTTCTTTGATTGTGCTGCAGAACGTTGAGGCTCTATT TGG CCAAATAGAGCCTCAACGTTCTGCAGCACAATCAAAGA AGG CGGATCCATGGTTAAAGATATTATCGAAAGGCATTTGC CCGCATGCGGCCGCTCAAGATGGTGTAAACGCACTAA GCG CACTAGCAGAACGTACTACGGTGTGGTTTATACAAGAG GGTTCCAGCTGAAGCTTCGTACGCT ... TCTAAGCTTGAGCTCTTTCACATAAGGGAGAGTCG CTGTATTCTATTTTTTCAAGAGTTCGATTCTATTG CAATAGAATCGAACTCTTGAAAAAATAGAATACAG CTTCTCTACGCCCGTGCTC GGAGGATCCGTCGACCATGACGACGACCAAGAG CCTTCTTTGATTGTGCTGCAGAACGTTGAGGCTCTATT...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... R&R Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Unknown Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial ... DNA sequence analysis [ 62] Rapid amplification of cDNA ends (RACE) Analysis by 5¢- and 3¢-RACE was performed using a SMARTTM RACE cDNA amplification kit (Clontech Laboratories, Palo Alto, CA, USA) ... in invertebrates J Mol Evol 48, 341– 347 Kawabata S, Nagayama R, Hirata M, Shigenaga T, Agarwala KL, Saito T, Cho J, Nakajima H, Takagi T & Iwanaga S (1996) Tachycitin, a small granular component...
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The Report of the Task Force on Financial Mechanisms for ICT for Development - A review of trends and an analysis of gaps and promising practices ppt
... dealing with small and relatively low-income countries in particular countries in sub-Saharan Africa, as well as in the Caribbean and Pacific islands, Central America or Caucasus and Central Asian ... Saudi Arabia and United Arab Emirates) and other donors (Israel and others) China and India also provide aid, but the numbers are not yet reported internationally 45 The members of the DAC are: ... Regional organizations and institutions can help facilitate cooperation and coordination and international financial institutions and donors can then play a vital role in seeding and facilitating the...
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Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity doc
... (Stratagene, La Jolla, CA, USA) The primers used were: 5¢-TATATCATTCA GGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3 ¢ (forward, the mutagenesis codon underlined) and 5¢-TT AGCGTATTCTAAAAGATACAAATAATCCTGAATGA ... Journal 27 6 (20 09) 622 7– 623 5 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 622 9 Structure of H pylori TenA N Barison et al A B Fig TenA active site (A) Cartoon view of a detail of TenA active ... aprE, at the transcriptional level [6] and, for that reason, TenA is often classified as ‘putative transcriptional regulator’ (http:// au.expasy.org/) A comparative analysis of several fully sequenced...
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