... 5'-ATCCAAGACGGAATTCGTTTTCCTGATTCTGGAG-3' The UBA2 gene fragment was amplified from plasmid pcDNA3.1HHR2 3A by PCR The 5' primer used was 5'ATCCAAGACGGAATTCACGCCGCAGGAGAAAGAAGCTATAG-3'; the 3' primer for the APOPEC3G-UBA2 ... proteasome-interacting factors Rad23 and Rpn10 of Saccharomyces cerevisiae Genetics 19 99, 15 3 (1) :69-79 Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F: ... the APOPEC3G-UBA2 fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGGAGGAAGTTGGCAG-3'; and the 3' primer for the APOPEC3G-UBA2* fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGagcGAAGTTGGCAG-3' Purified PCR products...
... text for details), although it was not detected by the immunological assays Psb P Cyanobacteria Glaucophyceae Red algae Diatoms Haptophyceae Brown algae Prasinophyceae Euglenophyceae Green algae ... Cyanobacteria Synechocystis sp PCC6803 Rhodophyceae (red algae) Cyanidioschyzon merolae Nuclear DNA Chloroplast DNA Cyanidium caldarium Chloroplast DNA Bacillariophyceae (diatoms) Thalassiosira pseudonana ... pseudonana Nuclear DNA Chloroplast DNA Odontella sinensis Chloroplast DNA Prasinophyceae Mesostigma viride Chloroplast DNA Euglenophyceae Euglena gracilis Chloroplast DNA Chlorophyceae (green algae)...
... inhibitors in cancer therapy Cancer Cell 2002, 1: 117 -12 3 Baselga J: The EGFR asa target for anticancer therapy – focus on cetuximab Eur J Cancer 20 01, 37:S16-22 Tamm I, Dorken B, Hartmann G: Antisense ... reading of the manuscript References 10 11 12 13 14 15 16 17 18 McLennan G., Roder DM: Lung cancer in Australia Med J Aust 19 89, 15 0:206-207 Burton RC: Cancer control in Australia: into the 21 ... http://www.gvt-journal.com/content/3 /1/ 5 genomic DNA, total RNA was treated with DNase I (Invitrogen, USA) for 15 at room temperature The reaction was stopped by addition of 25 mM EDTA and heated at 65°C for 10 followed...
... increased TEWL and decreased skin capacitance in areas adjacent to the application sites, as far as approximately 0.75cm away (Patil, Singh, & Maibach, 19 95) It appears that SLS acts in a time-and-dose ... technical aspects of this project For Dr Ravi Sahu, Dr Mohammed Al-Hassani, Dr Sarita Sehra, Dr Simarna Kaur, Qiaofang Yi, Davina A Lewis, Badri M Rashid, Evelyn T Nguyen, and Pamela Durant And finally, ... suprabasal keratinocytes in a complex biochemical cascade involving phosphatases and proteases into the active form filaggrin (FLG) (Koch, et al., 2000) FLG has a high affinity for keratins and...
... 2.2 .11 Homovanillic acid (HVA) assay This is a peroxidase-based assay using homovanillic acid (HVA; Mr = 18 2 .18 ) as the oxidizable substrate (Fig 3 .10 ) HVA was dissolved in water to give a 10 ... mixture was measured at λexcitation = 312 nm and λemission = 420 nm (Barja, 2002) 2.2 .12 p-Hydroxyphenyl acetic acid (HPAA) assay In this assay, p-hydroxyphenyl acetic acid (HPAA; Mr = 15 2 .15 ) was ... quenchers (AAQs) 13 0 xi LIST OF ABBREVIATIONS AND KEYWORDS AA Ascorbic acid AAQ Ascorbic acid quencher ABTS 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid AscH- Ascorbate CuZnSOD Copper and zinc-containing...
... convertases or C5 convertases [13 ] Asa result, C 5a may be generated via thrombin-mediated coagulation abnormalities that have been documented in anorexia nervosa patients [17 ,18 ] In addition, phacocytic ... levels in anorexia nervosa: apotentialbiomarkerfor the severity of disease? Annals of General Psychiatry 2 011 10 :16 Submit your next manuscript to BioMed Central and take full advantage of: ... between anorexia nervosa patients and healthy volunteers Statistical analysis Baseline characteristics of the study participants are described with mean and standard deviation (SD) or percentages...
... left breast He also had a significant family history forbreastcancer including a maternal grandmother, two of his maternal aunts and a maternal first cousin diagnosed with breastcancer On examination, ... situ disease to invasive disease, signifying that what appears to be DCIS on histology may potentially cause distant metastases [11 ] The lack of an intact basal myoepithel cell layer can be identified ... http://www.jmedicalcasereports.com/content/3 /1/ 13 10 11 12 13 14 15 male patient diagnosed by core needle biopsy: a case report Breast 2005, 14 :322-324 Andrés B, Aguilar J, Torroba A, Martínez-Gálvez M, Aguayo...
... Biophys Acta 19 99, 14 30 :11 9 -12 6 Herceg Z, Wang ZQ: Functions of poly (ADP-ribose) polymerase (PARP) in DNA repair, genomic integrity and cell death Mutat Res 20 01, 477:97 -11 0 Ariumi Y, Masutani M, ... studies have shown that upon DNA damage, PARP -1 (a nuclear enzyme which catalyzes the polyADP-ribosylation of target proteins in response to DNA damage) and Ku proteins are rapidly activated and compete ... Wilkie AOM, Lamb J, Harris PC, Finney RD, Higgs DR: A truncated human chromosome 16 associated with α thalassaemia is stabilized by addition of telomeric repeat (TTAGGG)n Nature 19 90, 346:868-871...
... (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, ... Chem 275, 10 995 11 0 01 Tanaka Hall TM, Porter JA, Beachy PA & Leahy DJ (19 95) Apotential catalytic site revealed by the 1. 7Angstrom crystal structure of the amino-terminal signalling domain of Sonic ... but as an apparent ligand for Patched Proc Natl Acad Sci USA 96, 10 992 10 999 35 Henikoff S & Henikoff JG (19 93) Performance evaluation of amino acid substitution matrices Proteins 17 , 49– 61 36...
... all preparations for ultrasound treatment as treated animals: anesthesia was administered and maintained at - 2.5% isoflurane/oxygen, scrotal fur was shaved, a ligature was used to retain the testes ... transplantation Proc Natl Acad Sci USA 19 94, 91: 113 03 -11 307 13 Clouthier DE, Avarbock MR, Maika SD, Hammer RE, Brinster RL: Rat spermatogenesis in mouse testis Nature 19 96, 3 81: 418 -4 21 14 Franca LR, ... transmitted diseases [1] , it is not always accepted asa family planning method for committed, monogamous couples [1, 2] Ultrasound’s potentialasa male contraceptive was first reported by Fahim...
... labeled anti-beta2 microglobulin (L368) for sHLA-I and L.2.03 Mab for sHLA-II were added to each bead and incubated for an additional hour at 45°C After additional washes, the color reaction was started ... therapy, with a surrogate marker, may have additional value as particularly effective immunomodulatory activity, such as that observed with natalizumab, may have apotential downside [26] that ... of ranks to avoid the assumption of normality implicit in the analysis of variance J Amer Statist Assoc 19 37, 32:675-7 01 Stone LA, Frank JA, Albert PS, Bash CN, Calabresi PA, Maloni H, McFarland...
... transplantation was performed days after the harvest Under anesthesia, the right knee joint was approached through a medial parapatellar incision, and the patella was dislocated laterally Full-thickness ... increasing the safety and economic feasibility Our study will advance and extend the clinical application of MSC-based cell therapy for cartilage injury Materials and methods Rabbits Skeletally mature ... non-cartilage Noncartilage only Matrix-staining (metachromasia) Normal (compared with host adjacent cartilage) Slightly reduces Markedly reduced No metachromatic stain Surface regularitya Moderate (1/ 2...
... flexibility [ 21] Increased HRV has been used asa marker of increased vagal activity and has been consistently associated with greater capacities to regulate stress, emotional arousal, and attention ... Rehabilitation 2008, 15 :386-396 doi: 10 .11 86 /17 49-8090-5-36 Cite this article as: Dao et al., Autonomic cardiovascular dysregulation asapotential mechanism underlying depression and coronary artery ... Depression and cardiovascular disease Circulation 2005, 11 1:250-253 12 Barnes RF, Veith RC, Borson S, Verhey J, Raskind MA, Halter JB: High levels of plasma catecholamines in dexamethasone-resistant...
... MCH, mean corpuscular hemoglobin; PT, prothrombin time; PTT, partial thromboplastin time; AST, aspartate aminotransferase; ALT, alanine aminotransferase; BUN, blood urea nitrogen Page of (page number ... Ichimura S, Sasaki R, Takemura Y, Iwata H, Obata H, Okuda H, Imai F: The prognosis of idiopathic portal hypertension in Japan Intern Med 19 93, 32:4 41- 444 Harmanci O, Bayraktar Y: Clinical characteristics ... microsomes (ALKM -1) , antimitochondrial antibody (AMA), and anti-liver cytosol antigen antibody (ALC -1) , were negative The serum gammaglobulin level was normal A gluten free diet was advised At a month...
... type isolated from the spinal cord of a cow with astasia Archives of virology 2000, 14 5 (11 ):2363-2370 Redaelli M, Cavaggioni A, Mucignat-Caretta C, Cavirani S, Caretta A, Donofrio G: Transduction ... anterior and 1. 5 mm lateral to the bregma, 3.7 mm below the pial surface Injection was carried out for 16 minutes and was performed using a Hamilton syringe Animals were monitored daily for neurological ... therapy in patients with recurrent glioblastoma multiforme: biological and clinical results Cancer gene therapy 2005, 12 (10 ):835-848 doi :10 .11 86 /17 43-422X-7-298 Cite this article as: Redaelli...
... Rockford, IL) and the membranes were probed with specific monoclonal antibodies against GFAP at a concentration of 1: 1000, mouse antiCaspase antibody at a concentration of 1: 1000 as primary antibodies ... whole and activated caspase-3 Lanes -1, expression of procaspase-3 in normal astrocytes transduced with HIV -1 Nef particles, 2-astrocytes treated with 10 mM glucose and HIV -1 Nef virus, 3-astrocytes ... of caspase-3 was further enhanced by the addition of HIV -1 Nef [34] The combination of hyperglycemia and Nef further activated the caspases in astrocytes (Figure 5B lane 6) as well as in diabetic...