affinity purification of mbp lane 1 prestained protein marker biorad lane 2 uninduced cell extract lane 3 induced cell extract lane 4 flow through from the load lane 5 flow through from column wash lane 6 proteins bound to chiti
Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
... NR (20 08) Solution structure of a double mutant of the carboxy-terminal 3 31 0 53 54 55 56 57 58 59 60 61 62 63 64 65 dimerization domain of the HIV -1 capsid protein Biochemistry 47 , 22 89 22 97 ... virus -1 capsid protein J Mol Biol 31 8 , 51 9 5 31 25 del Alamo M, Neira JL & Mateu MG (20 03) Thermodynamic dissection of a low-affinity protein- protein Dynamics of monomeric CAC 26 27 28 29 30 31 32 33 ... J Mol Biol 32 2 , 13 7 15 2 49 Tjandra N, Feller SE, Pastor RW & Bax A (19 95) Rotational diffusion anisotropy of human ubiquitin from 15 N NMR relaxation J Am Chem Soc 11 7, 12 5 62 12 56 6 50 Guignard...
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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... 2. 6H Tyr50 3. 5H Tyr50 2. 6H Tyr50 3. 5H Tyr50 2. 6H Tyr50 3. 5H Tyr50 2. 6H Tyr50 3. 5H Tyr50 b1 Tyr50 b2 Tyr50 b1 Tyr50 b2 Tyr50 b1 Tyr47 b2 Tyr47 3. 5H Tyr47 3. 5H Tyr47 cH Thr 55 cH Thr 55 a1 Gly 56 a1 ... Gly 56 a1 Gly 56 a2 Gly 56 a2 Gly 56 a Ala59 a Ala59 b Ala59 b Ala59 a1 Gly 56 a1 Gly 56 a2 Gly 56 a2 Gly 56 b Glu58 b Glu58 b1 Asp 52 b2 Asp 52 (Val57, Leu 61 , Leu 63 , Leu 64 , Leu67, Leu68 and Ile 74) are located ... [28 ] During peptide synthesis, 22 labeled amino acids ( 95% 15 N, 15 % 13 C) were introduced: Thr19, Leu20, Leu 22, Leu 23, Leu 26, Ala30, Phe 34, Leu39, Gly 43 , Tyr47, Ala 55, Ala59, Leu60, Ile 61 , Ile 63 , ...
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Báo cáo sinh học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx
... 1. 00mM Lipid 2. 0 0 .20 0 . 15 0 .10 0. 05 0.00 750 1. 5 1. 0 0 .5 0 . 25 mM Lipid 0 .50 mM Lipid 0.75mM Lipid 1. 00mM Lipid 0 .5 30 0 30 0 35 0 40 0 45 0 50 0 55 0 60 0 65 0 700 750 32 5 35 0 37 5 40 0 42 5 45 0 47 5 Wavelength ... 20 05, 86 : 13 1 - 13 8 Page 13 of 14 (page number not for citation purposes) Virology Journal 20 07, 4 : 12 3 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 Dalgleish AG, Chanh ... 0 . 25 0.00 50 0 55 0 60 0 65 0 700 0 .4 0 .2 55 0 0 .1 55 0 60 0 65 0 700 65 0 700 750 50 0 55 0 60 0 65 0 700 750 Wavelength (nm) F 0 . 25 2. 5 LLP -3 alone 0 . 25 mM Lipid 0 .50 mM Lipid 0.75mM Lipid 1. 00mM Lipid 2. 0...
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Báo cáo hóa học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx
... 1. 00mM Lipid 2. 0 0 .20 0 . 15 0 .10 0. 05 0.00 750 1. 5 1. 0 0 .5 0 . 25 mM Lipid 0 .50 mM Lipid 0.75mM Lipid 1. 00mM Lipid 0 .5 30 0 30 0 35 0 40 0 45 0 50 0 55 0 60 0 65 0 700 750 32 5 35 0 37 5 40 0 42 5 45 0 47 5 Wavelength ... 20 05, 86 : 13 1 - 13 8 Page 13 of 14 (page number not for citation purposes) Virology Journal 20 07, 4 : 12 3 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 Dalgleish AG, Chanh ... 0 . 25 0.00 50 0 55 0 60 0 65 0 700 0 .4 0 .2 55 0 0 .1 55 0 60 0 65 0 700 65 0 700 750 50 0 55 0 60 0 65 0 700 750 Wavelength (nm) F 0 . 25 2. 5 LLP -3 alone 0 . 25 mM Lipid 0 .50 mM Lipid 0.75mM Lipid 1. 00mM Lipid 2. 0...
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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps
... 40 66 6 .5 13 26 49 .7 88 42 0 .5 44 66 9 .4 61 9 36 .8 35 9 02. 7 21 7 0 42 . 6 6 15 20 .8 83 41 9 .5 13 3 810 .7 20 67 54 . 5 30 888 .4 32 5 82. 9 7 13 78 .2 3 41 8 7 36 959 .8 12 817 4 6 63 6 7.7 9 710 8 .2 21 0 710 56 3 32 6 9 93 15 4 92 41 1 25 97 6 35 10 828 55 ... 13 0 .28 20 .22 30 . 16 40 .22 12 0 . 24 30 .29 12 6. 31 50 .27 15 8. 31 40 .29 50 . 24 12 6 .22 70 . 25 18 .9 18 .30 8 .20 12 .8 26 .60 16 .6 16 .7 12 5 .4 5. 6 5 .1 11. 1 23 .00 38 .8 11 .6 58 .1 18 .60 6 .2 24 .2 11 .5 18 4 52 9 .4 4 066 6 .5 ... 10 828 55 9 7 14 27 2 27 80 73 61 7 43 4 727 45 0 7 35 3 29 6 6 45 45 059 41 27 47 7070 33 80 43 3 36 9 42 4 4 14 0 43 7 83 3 05 840 91 45 060 07 21 3 61 0 0 50 31 8 77 19 92 31 4 2 32 ( 31 0 0) (4 0 0) (7 0 0) (8 0 0) 11 (10 0 0) (6 0 0) (6 0 0) 14 ...
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Báo cáo y học: "What does the structure-function relationship of the HIV-1 Tat protein teach us about developing an AIDS vaccine?" ppsx
... responses Mol Biol Cell 20 04, 15 : 23 47 - 23 60 Page 10 of 13 (page number not for citation purposes) Retrovirology 20 09, 6 :50 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Fittipaldi A, ... Watkins DI: Tat-vacci- Page 12 of 13 (page number not for citation purposes) Retrovirology 20 09, 6 :50 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 11 4 11 5 11 6 11 7 11 8 nated macaques not control ... Effect of antibody to HIV- http://www.retrovirology.com/content /6 /1/ 50 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Tat protein on viral replication in vitro and progression of HIV -1 disease...
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Analysis of transport phenomena and electrochemical reactions in a micro PEM fuel cell with nature inspired flow field design
... ⎛ T ⎞ ⎛ 10 1 32 5 ⎞ DO2 = 3 .2 × 10 5 ⎜ ⎟ ⎜ ⎟ ⎝ 35 3 ⎠ ⎝ P ⎠ ( 35 ) 32 DH ⎛ T ⎞ ⎛ 10 1 32 5 ⎞ = 1. 1 × 10 ⎜ ⎟ ⎜ ⎟ ⎝ 35 3 ⎠ ⎝ P ⎠ 4 ( 36 ) 2. 2 .6 Cell power and efficiency Once the cell potential is determined ... [6, 7]; cW = 0.0 43 + 17 .81a − 39 .85a + 36 .0a cW = 14 .0 + 1. 4( a − 1) cW = 16 .8 (0 < a ≤ 1) (1 < a ≤ 3) (a ≥ 3) ( 25 ) ISSN 20 76 -28 95 (Print), ISSN 20 76 -29 09 (Online) 2 0 15 International Energy & ... Volume 6, Issue 1, 2 0 15 , pp .1- 16 13 Figure Velocity profiles in the mid-plane of the cathode (red) and anode (green) gas flow channels ISSN 20 76 -28 95 (Print), ISSN 20 76 -29 09 (Online) 2 0 15 International...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... Anti-p 24 kDa 55 95 43 36 36 28 34 28 17 26 6xHIS C B p 24 6xHIS RTB p 24 Figure of recombinant proteins expressed in E coli and immunodetection Purification Purification of recombinant proteins expressed ... with a booster step for diagnosis and monitoring of human 15 16 19 20 21 22 23 24 25 immunodeficiency virus type infection J Clin Microbiol 20 03, 41 : 1 0 16 -10 22 Pajot A, Schnuriger A, Moris A, Rodallec ... 72 28 55 BL 21 Ros2 T0 HMS Ros2 Ros-gami HMS LysE - h+ M 5h B T0 HMS Rossetta - h+ + T0 h h 5h 72 M 55 Ros-gami E BL 21 Ros2 HMS Ros 95 2 2 72 NaCl 55 50 mM T0 - h+ M 5h C T0 - h+ + T0 h h 5h 15 0...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... 24 1. 2. 2 .3 Epigenetic Regulation of Pluripotency 24 1. 2. 3 Key Transcription Factors Regulate Pluripotency 27 1. 2. 3 .1 Oct4/Pou5f1 27 1. 2. 3 .2 Sox2 28 ... 12 1 5 .1 Identification of Purified Proteins by Mass Spectrometry 12 1 5 .1. 1 How MS and MS/MS work 12 1 5 .1. 2 Preparing Proteins for Mass Spectrometry 12 2 5 .1. 3 Peptide ... 13 0 6 .1. 3 Peptide Identification by hybrid approaches 13 1 6 .1. 4 Peptide Identification by database searches 13 2 6 .2 From Peptides to Proteins 13 2 6 .2 .1 Choice of...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... T47-Hb-Q 61 - CH2c A50-CH3b-P 75- CH2d G 54 - CH2a-W 76- Ha H 72- Hd-V78-Ha H 72- Hd-V78-CH3c H 72- He-V78-CH3c H 72- He-L 81- Hc H 72- He-L 81- CH3d 1. 20 5 4. 458 4. 43 0 4. 43 0 1. 50 9 4. 070 7 .3 32 7 .3 32 8 .6 41 8 .6 41 8 .6 41 2 . 13 9; ... Disallowed 6 04 14 7 22 3 21 9 15 0.0 0 25 ± 0.00 06 0 . 25 nm 0.0 21 nm heavy atoms) 0 .10 0 ⁄ 0 . 14 9 0. 027 ⁄ 0.0 81 0.0 74 ⁄ 0 . 14 1 0. 030 ⁄ 0.0 91 0. 0 16 ⁄ 0. 0 46 0. 049 ⁄ 0.088 58 % 31 % 6% 5% a The sum of all NOE ... 2 . 13 9; 1. 7 74 2 .10 9; 2. 45 7; 1. 8 46 ; 4. 777 4 .20 7 1. 017 1. 017 1. 7 25 0. 933 2. 0 64 2. 2 32 2 .4 61 1. 940 Calculation of the VpUcyt structure in micelle solution employed 6 04 upper distance limits derived from...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... AAAGAGATG 1. 50 E-008 1. 10E-008 1. 30 E-008 1/ ΔF 1. 30 E-008 1. 20 E-008 1. 28 E-008 1. 00E-008 25 50 75 10 0 0 .10 0 0.0 95 0.090 0.0 85 1. 26 E-008 AAAGACACG Kd =4 nM 0 .10 5 Kd =1. 2 nM 1/ ΔF 340 1/ ΔF 340 0 .11 0 Kd=0 .29 nM 1. 32 E-008 ... ENSG000000 0 34 00 Caspase -10 10 1 6 75 8 14 8 01 35 5 31 5 9 63 808 41 8 26 2 6 51 7 25 857 caspase -10 gene, four variant motifs were identified Among them, AAACAGATG () 25 4 to ) 26 2) is present in the positive ... caspase -1 orthologs from Pan troglodytes (DOOP ID: 8 3 12 31 4 5, ) 24 5 to ) 25 3) and Macaca mulatta (DOOP ID: 9 42 5 28 93, ) 24 5 to ) 25 3) The motif sequences of the caspase -3 gene (5 -AAAGAGATG -3 , ) 828 to...
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Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc
... G 22 G 22 S 41 Without OG S 12 S 45 S48 S 54 S 55 T 26 V29 M1 Q9 M3 V 16 W6 D10 R31V 21 V 32 M 25 L20 R4 F 52 R 42 R 53 V 35 W49 S3 R 36 A5 A 44 I39 C 13 A 43 A 23 I 33 A2 W 34 A 56 With OG 6% 11 8 V 21 122 V 35 12 6 I 33 ... 13 0 13 0 10 .0 8 .4 A17 8 .2 7.8 1H (p.p.m.) 8 .4 8 .2 8.0 7.8 C C0 1 2 C α 1 2 C β 1 2 11 1. 0 0 .5 SSP score Δδ (p.p.m.) B A17 10 .0 13 0 8.0 W 34 0.0 –0 .5 1. 0 21 31 41 51 Amino acid sequence 11 ... of HCV ARFP/Core +1/ S kDa Absorbance at 28 0 nm (a.u.) A 67 43 29 A Boumlic et al B kDa (a) 13 .7 6 .5 NusA 55 28 12 11 (b) 55 28 HCV-1a Core +1/ S 11 (c) 55 28 20 40 60 80 a 10 0 HCV-1b Core +1/ S 11 ...
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Báo cáo khoa học: End-damage-specific proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx
... (20 01) DNA synthesis and dRPase activities of polymerase beta are both essential for single-nucleotide 57 62 15 16 17 18 19 20 21 22 23 24 25 26 27 28 patch base excision repair in mammalian cell ... Nucleic Acids Res 22 , 18 66 18 73 11 Parsons JL, Dianova II & Dianov GL (20 04) APE1 is the major 3 -phosphoglycolate activity in human cell extracts Nucleic Acids Res 32 , 35 31 35 36 12 Rasouli-Nia A, ... recruitment of DNA ligase IIIa–XRCC1 to sites of base excision repair Biochemistry 44 , 10 6 13 10 61 9 FEBS Journal 27 2 (20 05) 57 53 57 63 ª 20 05 FEBS J L Parsons et al 29 Wilson SH & Kunkel TA (20 00) Passing...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... Angiotensin II 17 28 24 25 26 27 28 29 30 31 32 33 34 35 enhances interleukin -18 mediated inflammatory gene expression in vascular smooth muscle cells: a novel cross-talk in the pathogenesis of atherosclerosis ... Han et al +1 AP -1 STAT5 tataaa 28 2~ 27 7 17 2~ 16 3 TATA AP -1 – 41 9 ~– 4 12 B Relative luciferase activity 90 * 80 70 60 50 40 * 30 * 20 Discussion 10 In this report, we demonstrated, for the first time, ... (20 01) AP -1 in cell proliferation and survival Oncogene 20 , 23 90 24 00 16 van Dam H & Castellazzi M (20 01) Distinct roles of Jun : Fos and Jun : ATF dimers in oncogenesis Oncogene 20 , 24 53 24 64 ...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... MG1 32 Lactacystin DMSO MG1 32 pHPI - 15 07 pHPI - 14 94 Control pHPI - 14 95 MG1 32 pHPI - 14 95 (a) B 20 14 pHPI - 15 79 +MG1 32 pHPI - 15 79 pHPI - 14 96 +MG1 32 pHPI - 14 96 Control C anti-core +1 anti-core pHPI - 15 80 ... ª 20 07 FEBS 9– 8 25 pHPI -17 48 (pBluescript II KS-) pHPI - 15 80 (pA-EUA2) 59 0– 828 59 0– 8 25 51 5 – 8 25 3 42 8 25 pHPI - 15 09 (pcDNA3 .1( –) ⁄ Myc-His) pHPI - 14 96 (pA-EUA2) pHPI - 15 79 (pA-EUA2) 9– 8 25 pHPI -17 06 ... C 53 C2 03 60 N2 94 N2 95 66 N 2 46 –N 247 59 N298–N300 57 N298–N 222 C 53 C 54 64 60 For the PCR amplification of the core ⁄ core +1 sequences, the following conditions were used: 94 °C for followed by 35 cycles...
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Báo cáo sinh học: " Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis" ppt
... virus type Vpu protein is an oligomeric type I integral membrane protein J Virol 19 93, 67 :50 56 -50 61 http://www.virologyj.com/content /4 /1/ 81 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Moore PB, ... 4: 81 10 11 12 13 14 15 16 17 18 19 20 21 22 Cohen EA, Terwilliger EF, Sodroski JG, Haseltine WA: Identification of a protein encoded by the vpu gene of HIV -1 Nature 19 88, 33 4: 5 32 - 5 34 Schubert U, ... glycoprotein enhancement of particle budding: role of the cytoplasmic domain J Virol 19 96, 70 : 26 69 - 26 73 Page 10 of 11 (page number not for citation purposes) Virology Journal 20 07, 4: 81 10 11 12 13 ...
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Báo cáo hóa học: " Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed β-propeller domain of the HIV-1 cellular interactor EED" pptx
... integration Science 20 03, 30 0 :17 49 -17 51 http://www.virologyj.com/content /5 /1/ 32 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Engelman A: The ups and downs of gene expression ... 2C, D) These regions spanned residues 98 10 6 (1) , 12 8 13 3 (2) , 22 3 22 8 (3) , 26 8 27 3 (4) , 29 4 30 5 (5) , 31 4 – 31 9 (6) , 3 82 38 7 (7) and 43 4 – 43 9 (8), respectively Five of these regions, n° 2, 4, 5, and ... structure of the Drosophila extra sex combs repressor protein Mol Cell Biol 19 97, 17 :66 63 - 66 72 Page of (page number not for citation purposes) Virology Journal 20 08, 5: 32 10 11 12 13 14 15 16 17 18 19 ...
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Báo cáo hóa học: " The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2" pot
... Page 12 of 13 (page number not for citation purposes) Virology Journal 20 08, 5 : 12 5 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 not essential for virus replication in cell culture J Virol 19 96, ... wild-type YK3 04 and MT1 03 Page of 13 (page number not for citation purposes) Virology Journal 20 08, 5 : 12 5 http://www.virologyj.com/content /5 /1/ 1 25 A 10 8 (PFU/ml) 10 6 10 4 10 2 10 0 10 20 30 40 50 (hour ... MT1 02 MT1 03 MT1 03 MT1 02 WT Plaque size (x40, cm) C Figure Comparison of the phenotype of wild-type YK3 04 and the recombinant viruses MT1 02 and MT1 03 Comparison of the phenotype of wild-type YK304...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... characterization of the recombinant BaeyerVilliger monooxygenase MekA from Pseudomonas veronii MEK700 Appl Microbiol Biot 77 : 12 51 12 60 doi :10 .10 07/s0 0 25 3- 007 - 12 64 - 6 doi :10 .11 86/ 21 9 1-0 855 -1- 13 Cite this ... (+)-Camphor ((+) -1) 66 0.9 (-)-Camphor ((-) -1) 25 0 .3 Norcamphor ( 13 ) 98 1. 3 (±)-cis-bicyclo [3 .2. 0] hept -2- en -6- one ( 14 ) 10 0 1. 4 (R,R)-bicyclo [2. 2 .1] heptane -2, 5- dion ( 15 ) 94 0. 06 a Conversion ... investigation of the Kadow et al AMB Express 2 011 , 1: 13 http://www.amb-express.com/content /1/ 1 / 13 Page of 4- hydroxyacetophenone monooxygenase from Pseudomonas putida JD1 Appl Environ Microb 75: 31 0 6 31 1 4...
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