adenoviral gene expression and replication in human tumor explant models
báo cáo khoa học: " Gene expression and hypoxia in breast cancer" doc
... For example, Winter et al [47] defined an in vivo hypoxia ‘metagene’ (signature) in head and neck squamous cell carcinomas (HNSCCs) by clustering (that is, by finding) genes whose expression pattern ... use, including the Topoiso merase inhibitor topotecan, have been shown to inhibit HIF‑1α protein accumulation in cell lines and xenograft studies [49,50] It may be that, in the clinical setting, ... E1B 19 kDa protein-interacting protein 3; CAD, carboxy-terminal transactivation domain; CAIX, Carbonic anhydrase 9; CDKN1A, Cyclin-dependent kinase inhibitor 1A; FIH, factor inhibiting HIF; GLUT1,...
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Báo cáo y học: "Vascular Endothelial Growth Factor (VEGF) isoform expression and activity in human and murine lung injury" pps
... Several lines of in vitro evidence have pointed to a possible role for VEGF in lung repair and recovery following injury[19,29,35,36] In one LPSinduced murine model of lung injury, intrapulmonary ... depicting 3ng/ml and combination confirming a significant and cell number (B) following treatment for 48 hours with Bar Bar graphs depicting 3H-thymidine incorporation into ATII cells (A) and cell ... Human VEGF lung expression and quantification in normal, early and late ARDS Human VEGF expression was noted on alveolar epithelium and macrophages with weaker endothelial expression in all human...
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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx
... factor, insulin-like growth factor-binding proteins), morphogens, enzymes, numerous cytokines and chemokines, interleukins, and so forth [52] It can be hypothesized that a stringent control of the gene ... IL-17 in RA decreased the gene expression of both HexB and GusB, whereas in OA it did not have an effect Finally, we were interested in whether gene expression of the glycosidases was influenced ... proinflammatory cytokines and chemokines [13] Page of 13 (page number not for citation purposes) There is increasing evidence that SFs are key players in the pathogenesis of RA by invading and...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... containing DNA spots (5 lg each) corresponding to internal fragments of ADC or cruzipain genes The latter (used as a control) is a housekeeping gene that encodes the main cysteine proteinase ... segments corresponding to ADC [10] or cruzipain [20] genes The latter DNA was obtained by PCR amplification using a recombinant plasmid containing the cruzipain gene as template and primers A (5¢-ATGT ... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and...
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Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
... between acetylcholinesterases of kidney and erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin 4522 (LCA), and Ricinus communis agglutinin (RCA) (Fig ... acetylcholinesterase gene expression in human brain tumors Oncogene 21, 8428–8441 28 Calaf GM, Parra E & Garrido F (2007) Cell proliferation and tumor formation induced by eserine, an acetylcholinesterase ... Alzheimer’s disease in the brain in vivo Neurobiol Aging doi:10.1016/j.neurobiolaging.2010.04 028 26 Vidal CJ (2005) Expression of cholinesterases in brain and non-brain tumours Chem Biol Interact 157–158,...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... and control vs insulin and TNFa plus insulin vs insulin alone (C); **P < 0.01 when compared with insulin alone (B) and TNFa incubation when compared with control (C); ***P < 0.05, TNFa plus insulin ... PEPCK expression PEPCK overexpression, in turn, has been shown to attenuate insulin signalling and hepatic insulin sensitivity in transgenic mice [41,55] Interestingly, adipose selective overexpression ... on insulin action on PEPCK expression A Foxa2 IgG TN Fα In s TN Fα + C In s on Input 2.5 1.5 ** * 0.5 TN Fα + TN Fα s In In C s on Foxa2 binding normalized to input (arbitary units) B Foxa2 binding...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
... with a decrease in its protein, uPAR protein was analysed using western blotting Single and double dose transfections did not result in any significant decrease in uPAR protein, and therefore a ... domain and nine for each of the hybridizing arms They have the ability to bind specific uPAR mRNA by complementary base-pairing between the Dz arms and the sequences flanking the uPAR mRNA and ... drug It would be interesting to perform further studies in other cancer cell lines and in an animal model to show the efficacy of anti-uPAR DNAzymes in preventing cancer cell growth and metastasis...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment containing the N-terminal part of the gyrA gene To create a plasmid for integration of ... previously [17] Supercoiling assay DNA supercoiling was assessed in terms of the linking number of intracellular plasmid pBR322 [18] DNA supercoiling was expressed as the active linking number, aLk, ... the difference in linking number of pBR322 in the respective sample and of pBR322 isolated from cells incubated for 30 with 0.1 mgámL)1 of coumermycin and 0.2 mgámL)1 rifampicin Topoisomerase...
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Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation pot
... glutamine, lysine and histidine at the N-terminus and the nonpolar amino acid proline and the hydroxy amino acids serine and threonine at the C-terminal border 3842 Fig Spicule-associated protein SPIaP_ ... contain proliferating and differentiating cells [12,13] We found that, in response to selenium, the expression of two genes, selenoprotein M and the (sponge-specific) spicule-associated protein, ... range from amino acids 32–55 and 204–237 Recombinant proteins and antibodies To identify selenoprotein M and the spicule-associated protein in sponge extract, the respective recombinant FEBS Journal...
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Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx
... other [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule ... measured using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions Bovine serum albumin was used as standard to determine the protein ... quantitative polymorphism The remaining nine individuals fell in the normal (NN) category; seven in a narrow range between 16.2 mgÆdL)1 and 18.4 mgÆdL)1, and two with 20.5 mgÆdL)1 and 20.6 mgÆdL)1 Although...
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Báo cáo sinh học: "Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells" doc
... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
... By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order to grow unhindered in the ... by infiltrating immune cells Taken together, our data demonstrate that SARS-CoV in general is a weak inducer of cytokines and antiviral genes in non-lymphatic cells Chemokines like IP-10 and ... Plasma inflammatory cytokines and chemokines in severe acute respiratory syndrome Clin Exp Immunol 2004, 136:95-103 BGoNRPf SARS: Dynamic changes in blood cytokine levels as clinical indicators in...
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báo cáo hóa học:"Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells" potx
... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...
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Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf
... have shed light on factors involved in directing lymphocytes into inflamed tissue and maintaining inflammation in SS, whereas the etiologic factors of SS remain to be delineated Of potential importance ... the interaction between epithelial cells and infiltrating T cells has been characterized in detail, but the cytokines involved in local B cell activation remain largely unknown Chemokines The infiltration ... Immunoglobulin kappa chain receptor editing in systemic lupus erythematosus J Clin Invest 1998, 102:688-694 35 Dörner T, Farner NL, Lipsky PE: Immunoglobulin lambda and heavy chain gene usage in early...
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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx
... Kresse H: Decorin-type I collagen interaction Presence of separate core protein-binding domains J Biol Chem 1995, 270:8877-8883 Hedbom E, Heinegard D: Binding of fibromodulin and decorin to separate ... cartilage in both MTP and LTP, and there was a generalized decrease in staining following meniscectomy (Fig 2e–h) As expected [31,32], types I and III collagens were also prominently immunolocalized in ... control and meniscectomized joints and OA human cartilage are shown in Fig There was little difference in total staining intensity between nonoperated and meniscectomized cartilage for the 45 kDa intact...
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Báo cáo khoa học: " Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines" pot
... Innotech, San Leandro, CA) Fold change in protein expression was expressed as a ratio calculated by dividing the specific protein band density with the actin band density (loading control), and ... lysed in RIPA buffer (Santa Cruz Biotechnology, Inc Santa Cruz, CA) containing protease inhibitor cocktail and phosphatase inhibitors (Roche Applied Science, Indianapolis, IN) The samples were incubated ... nitrocellulose membrane using iBlot Dry Blotting System from Invitrogen (Carlsbad, CA) Non specific protein binding was blocked by incubating the membranes for hour in 3% blocking grade non fat dry...
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Báo cáo y học: "Peroxisome proliferator-activated receptor γ1 expression is diminished in human osteoarthritic cartilage and is downregulated by interleukin-1β in articular chondrocytes" doc
... expression of PPARγ in OA cartilage may, at least in part, be involved in increased expression of inflammatory and catabolic genes, promoting articular inflammation and cartilage degradation In addition, ... of the standard curve method Serial dilutions of internal standards (plasmids containing cDNA of target genes) were included in each PCR run, and standard curves for the target gene and for GAPDH ... mitogen-activated protein kinases and tyrosine kinases on IL1-induced NF-κB activation and iNOS expression in bovine articular chondrocytes Nitric Oxide 2002, 6:35-44 40 Liacini A, Sylvester J,...
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báo cáo khoa học: "Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cells" docx
... bladder carcinoma cell proliferation, we determined the effect of as-APF on the expression or activation of enzymes involved in winglessint (Wnt)/frizzled signaling (including AKR-transforming enzyme ... CA) containing Complete Protease Inhibitor Cocktail (Roche, Indianapolis, IN) and centrifuged at 14,000 rpm for 15 minutes at 4°C Supernatant containing total cell protein was collected and stored ... receptor for mediating APF antiproliferative activity in T24 bladder carcinoma cells APF increases p53 tumor suppressor gene expression via CKAP4 in T24 cells % Inhibition of H-Thymidine Incorporation...
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báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
... leupeptin, 1-μg/ml pepstatin, 1-μg/ml aprotinin and 1% Triton X-100) The insoluble material was pelleted (15,000 × g for 15 min) at 4°C Total protein content in the lysates was determined using ... would be interesting to determine if this behavior is a more general property of MICA- and MICB-producing cells by evaluating whether virus- infected and tumor cells known to secrete MICA and MICB ... would be interesting to determine if NK and other NKG2Dexpressing cells could also be induced to produce and secrete MICA and MICB If the secretion of MICA and MICB by virus-infected or tumor cells...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... Figure virus-induced HSE binding activity in macrophages and a human hepatoma cell line Vaccinia3 Vaccinia virus-induced HSE binding activity in macrophages and a human hepatoma cell line (A) Macrophages ... interleukin 12 and interleukin 10 expression in vaccinia virus-infected human monocytes and U-937 cell line Cytokine 2000, 12:900-908 Jindal S, Young RA: Vaccinia virus infection induces a stress ... vaccinia virus infection on IL-10 gene expression in human blood elutriated monocytes The finding stays in agreement with the data on the enhancement of the hsp90 gene expression by IL-10 in a human...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008