... (A) 3rd vis-à-vis, (V), 20× H&E staining of paraffin section of cranial part of the adenoH&E staining of paraffin section of cranial part of the adenohypophysis (A) and vis-à-vis, proliferating ... paraffin located S = stomatodeum, cones ryngeal area high-prismatic cells: horizontal epithelialA = adeH&E staining of paraffin section (4 μm) through adenopharyngeal area with basally located ... an antibody that specifically binds to the cleaved and thereby activated form form of caspase3 (Anti-ACTIVE Caspase-3 pAb; Promega, Cleaved Caspase-3 (Asp 175) Ab; Cell Signaling) Proliferating...
... mitochondria in this process [19] In mammalian apoptosis, caspase-8 and caspase-9 are known to be associated with the mitochondrial pathway Active caspase-8 induces the release of mitochondrial apoptosis ... mitochondria were most abundant in fraction (Fig 5A) On the other hand, acid phosphatase activity was higher in fractions and than in fraction (Table 1) These results indicate that fraction contains a ... by Lu and Wolfe [23], who used a combination strategy of 4,6-diamino-2-phenylindole (DAPI) staining for the detection of DNA and Azo dye staining for the identification of acid phosphatase activity,...
... Materials and Methods Zebrafish care and maintenance and tissue collection 1.1 Zebrafish maintenance Wild type and transgenic adult zebrafish were maintained in the National University of Singapore ... the zebrafish gaining popularity as a model organism in the study of human diseases including cancers, we aim to investigate the roleof autophagy in liver cancer using our established zebrafish ... Schematic illustration of the autophagy pathway and its core machinery in mammalian cells 17 Figure Regulation of mammalian autophagy 22 Figure Autophagy in tumourigenesis and anti-cancer therapy...
... Regional systems of innovation are increasingly being framed within an international arena The links between sub-regional, regional, national and international systems of innovations imply hat analyses ... main emphasis was put on strengthening research and highquality education The Commission proposed a standardisation and streamlining of teaching programmes, a large increase in doctoral programmes, ... requirements regarding staff qualifications and doctoral degree education Another proposal was the establishment ofa national agency in charge of evaluation and accreditation of study programmes and higher...
... identical to that of ePAD or AAH53724 (an egg and embryo abundant peptidylarginine deiminase) Mouse cortical granules contain PAD To ascertain if mouse cortical granules contain PAD, antibodies made ... such as chemokines, albumin, and transcobalamin II, are also secreted [40-42] PADs are a family of calcium dependent enzymes that catalyze the conversion of arginine into citrulline in proteins In ... orange nucleic acid stain and Alexa-488 conjugated to goat anti-rabbit IgG were obtained from Molecular Probes (Eugene, OR) PAD V (N) antibody was made against recombinant human PAD V and affinity...
... is largely written at a tenth grade reading level However, each action that a family caregiver should take is accompanied by a drawing showing a person carrying out that action along with a caption ... are large numbers of people with low literacy skills as shown in Table [8] Gazmararian et al [9] reported, ina study of 3260 enrollees ina national managed care organization in the United States, ... recall, pictures are present during both learning and recall A clinical example would be a health professional telling a patient or family caregiver how to treat a bed sore while viewing matching...
... 5¢-ACCACTCTCTGGATGTGATTGGA-3¢ and 5¢- TCAAGAACATTTTATTTCCCACATTTT-3¢ for Ugt2b5; 5¢-ATTGCCCATATGGTGGCCAAAGGAG-3¢ and 5¢- GGCTGCCACACAAGCGAGTAGGAAT-3¢ for Ugt2b37; 5¢-GGGAAGGACATGAAGGAGAGAGC-3¢ and ... 5¢-AGAGATGATCCCATGAGAAACGG TGAA-3¢ for Cyp 3a4 4; 5¢-AGATCATCATTCCTTGGCA CTGG-3¢ and 5¢- ATTGCAGAAAGGAGGGAAGATGG -3¢ for Cyp 4a1 0; 5¢-CCAGTTGAGTGACGAGGAG ATGG-3¢ and 5¢-TCTGCATGCCCTCAAATGTTACC-3¢ for Akr1b8; ... primers were as follows: 5¢-CCCCTTACAGCTCTG CTTCATT-3¢ and 5¢-TCAAGAATGGATACACATAAA CACAAGGA-3¢ for Cyp2c29; 5¢-CCAGCTCTGCTTCAT TCCTCTCT-3¢ and 5¢-CGCAGGAATGGATAAACATA AGCA-3¢ for Cyp2c38; 5¢-ACTTCTCTGTGGCAAGCCC...
... C45 8A( sense) 5¢-ATACTTGAGTGGGCTGTG TTATCAG-3¢; C45 8A( antisense) 5¢-ATCTGATAACAC AGCCCACTCAAGTAT-3¢; C46 6A( sense) 5¢-GATAAA GCACCCGCTATCACAGACTGG-3¢; C46 6A( antisense) 5¢-CCAGTCTGTGATAGCGGGTGCTTTATCTG-3¢; ... 5¢-ACTACCGAACGGGCCCCCGGGGTC AGACTGGACACATGG-3¢ and 5¢-TCGGGCCATGGC ATGCCCGGGGGTCAGAGCTGGG-3¢ for amplification of D4 of GCSFR and 5¢-TACTCTCAAGAAATG CCCGGGTCCCATGCCCCAGAG-3¢ and 5¢-GCCCAG GATGATGTGTAGCTCCCCGGGCTCTGGGGTCAA ... C49 1A( sense) 5¢-GCAGAGAGCAAAGCCTATTTGAT AACAG-3¢ and C491(antisense) 5¢-TGTTATCAAATAG GCTTTGCTCTCTG-3¢ PCRs were performed applying standard procedures All plasmids were sequenced using an ABI...
... 5'-TATAGTCGACCACCCGGACTCAGAGTCTCCT-3' and 5'-ATATGGATCCATCTCAGTCCCTCACAAGA-3' (HLAB), 5'-ACCTGTACGCCAACACAGTG-3' and 5'-GCCATGCCAATCTCATCTT-3' (β-Actin), 5'-CTGAAGGAGACCATTGGTGA and 5'-GGTACTGGTACACAGTTCGA-3' ... and 5'-GGACGTAGGGTAAACGTCAGC-3' (TAP2), 5'-CTCGCGCTACTCTCTCTT3' and 5'-AAGACCAGTCCTTGCTGA-3' (β2m), 5'-TATAGTCGACCACCCGGACTCAGAATCTCCT-3' and 5'ATATGGATCCATCTCAGTCCCTCACAAGA-3' (HLA -A) , 5'-TATAGTCGACCACCCGGACTCAGAGTCTCCT-3' ... β-actin and CD45, 59°C for TAP1 and 60°C for HLA -A and HLA-B The primer sequences were: 5'-GAGACATCTTGGAACTGGAC-3' and 5'-CTCTGAGTGAGAATCTGAGC-3' (forward and reverse, TAP1), 5'GTACAACACCCGCCATCAG-3'...
... Mano A, Falcao A, Godinho I, Santos J, Leitao F, de Oliveira C, Caramona M: CA-125 AUC as a predictor for epithelial ovarian cancer relapse Cancer Biomark 2008, 4:73-81 Mano A, Falcao A, Godinho ... estimation of female patients with relapsed ovarian carcinoma In multivariate analysis only the variation of blood levels of CA125 and the free disease interval from the finalization of the first line ... paclitaxel/cisplatin as primary treatment for advanced epithelial ovarian cancer: a National Cancer Institute of Canada Clinical Trials Group Study J Clin Oncol 2000, 18:4038-4044 Jacobs IJ, Skates SJ, MacDonald...
... of their HIV status Additionally, at that time, very little was known in the sub-Saharan African context about what role, if any, children play in decision making on research participation, and ... formative research was approved by the Institutional Review Boards at the Kinshasa School of Public Health in the DRC and at the University of North Carolina at Chapel Hill in the US All participants ... experiences among youth and parents and caregivers in non-research settings, as well as culturally appropriate approaches for disclosing an HIV diagnosis to minors as this information could inform our assent...
... Giardine B, van Baal S, Kaimakis P, Riemer C, Miller W, Samara M, Kollia P, Anagnou NP, Chui DH, Wajcman H, Hardison RC, Patrinos GP HbVar database of human hemoglobin variants and thalassemia ... these Hb variants are inherited in an autosomal dominant manner High affinity Hb variants release oxygen in the tissue relatively slowly and create relative tissue hypoxia This leads to increased ... easily available in routine and even reference laboratories Lichtman and colleagues have reported a mathematical formula which can be used to calculate P50 reliably [5] Calculating P50 using this...
... Kimura Y, Sakata M, Naganawa M, Oda K, Miyatake R, Fujisaki M, Shimizu E, Shirayama Y, Iyo M, Hashimoto K: High occupancy of sigma-1 receptors in the human brain after single oral administration of ... (’moderate akathisia’) Fluvoxamine (50 mg, twice a day) was administered Substantial relief of akathisia was noted on day of fluvoxamine treatment The dose of aripiprazole was increased to 24 mg, since ... with antipsychotic treatment Aripiprazole is an antipsychotic drug that acts as a partial agonist at dopamine D receptors and serotonin 5hydroxytryptamine (5-HT) 1A receptors, and an antagonist at...
... added to cultures at a final concentration of 10–50 µg/ml or 10 µl of concanavalin A (ConA) (Difco) at a final concentration of µg/ml These concentrations had optimal stimulatory effects as assessed ... 99:245-250 Sakata K, Sakata A, Vela-Roch N, Espinosa R, Escalante A, Kong L, Nakabayashi T, Cheng J, Talal N, Dang H: Fas (CD95)-transduced signal preferentially stimulates lupus peripheral T lymphocytes ... an allelic combination derived from the original inbred strains Arthritis Rheum 2002, 46:1067-1074 Sugiyama M, Tsukazaki T, Yonekura A, Matsuzaki S, Yamashita S, Iwasaki K: Localization of apoptosis...
... data in public databases such as dbGaP [13] for use in (disease sub-type-specific) meta-analyses ITGAV is the latest example ofa candidate gene that may be relevant to RA Increased significance ... Migliorini P, Balsa A, Westhovens R, Barrera P, Alves H, et al.: The ITGAV rs3738919-C allele is associated with rheumatoid arthritis in the European Caucasian population: a family-based study Arthritis ... between PADI4 and CTLA4, but not with other associations [12] With OR in the range 1.1 to 1.2, variants of PADI4 and CTLA4 confer a similar risk to that of the new ITGAV variant Because confirmed associations...
... Kit (Qiagen Inc., Valencia, CA, USA) from a small tail snip taken between and weeks of age just prior to weaning Each DNA sample was used as a template in polymerase chain reaction amplification ... presence of ANAs, in particular anti-SS -A/ Ro and antiSS-B/La in the sera of human patients, is one parameter in the diagnosis of clinical SjS Concomitantly with the appearance of mononuclear leukocytes ... recombinant founder pair was identified, the RI line was maintained via a single line of descent All RI lines were bred and maintained under specific pathogenfree conditions in the animal facility...
... Cardiovascular collapse due to anaphylaxis is a vasodilatory shock, characterized by an abrupt fall in systemic vascular resistance, enhanced vascular permeability, intravascular volume depletion and ... hypoxia and lactic acidosis can maintain all the described pathophysiologic mechanisms and induce a relative deficiency in vasopressin plasma concentration further amplifying the vasoplegic scenario ... postoperative normalization of plasma lactate levels Conclusion In case of anaphylactic shock, continuous infusion of lowdose vasopressin might be considered in the treatment algorithm after inadequate...
... ripening-associated patterns for aromatic and branched chain amino acid-, fatty acid-, and furanrelated classes, with a peak at the S3/Br stages and a more or less pronounced decline at later ripening ... The data set was made up of data from eight repetitions of each ripening stage of RH and RHB The variable set was made of the major 41 volatile aroma compounds PCA involves a mathematical procedure ... than RHB (Table 1) At full ripening S4 stage, xanthophylls made up the majority of carotenoids - zeaxanthin was the main carotenoid in RHB, while antheraxanthin, luteoxanthin and zeaxanthin were...