... revealed that upon peptide binding, NarJ undergoes a conformational change Isothermal titration calorimetry (ITC) and BIAcore analysis showed that protonation ofthe chaperone is responsible for ... the interaction (on rate constant kon and off rate constant koff) between NarJ and the NarG(1–15) peptide Taking into account the existence of two subpopulations of NarJ at pH 7, the BIAcore ... basis for peptide recognition by NarJ S Zakian et al Upon peptide binding, NarJ undergoes a conformational change AThe temperature dependence ofthe partial molar heat capacity of free NarJ or NarJT...
... is clearly responsible forthe enhanced inhibitory potency Conformational alterations ofthe peptide backbones have to be taken into consideration especially in the case of different amino acid ... molecules one of them bound in the active site and one of them at an alternative site This special, rare type of inhibition is therefore worth examining although the kinetic constants characterize these ... binding site Therefore, the substitution of only one amino acid (Asp2) in the Tat(1–9) sequence resulted in a change ofthe inhibition mode in conjunction with a gain ofthe ability to bind at the...
... substitution of Arg46 on intracellular signalling The stimulation of accumulated InsPs by increasing concentrations of AVP, was assayed for each ofthe 19 mutant constructs and the dose–response characteristics ... substitution of Arg46 facilitated agonist-induced transition ofthe V1aR to the active state, signalling was nevertheless still dependent on agonist because an increase in basal signalling was not ... mutation of Arg46 had the dual effect of (a) decreasing agonist affinity and (b) promoting the agonist-induced active conformation due to the loss ofa stabilizing constraint onthe ground state of the...
... Stratagene The sequence ofthe oligonucleotides forthe mutations at the underlined positions were: 5¢-ACATGGC AACAGGCGGAATCAGGTAAA-3¢ (W3 4A) and 5¢-AT ATTCTAGAAAGCTAACCAGAATCAA-3¢ (Y7 4A) The ... (1996) Characterisation of independent DNA and multiple Zn -binding domains at theNterminusof human DNA-(cytosine-5) methyltransferase: modulating the property ofa DNA -binding domain by contiguous ... many proteins; the fusions found may simply represent permutations and combinations ofa set of common components and may not imply interactions [37] Even though the organisms that harbor these...
... spontaneously under a range of conditions raises important questions about the mechanism of cleavage and suggests that cleavage may occur in vivo If it is an in vivo phenomenon, truncation of ... CAACGCATAC-3¢; truncate 4, 5¢-GGGAATTCCATAT GAGCAACGCATACGACGCCGGCATC-3¢; truncate 5, 5¢-GGGAATCCATATGAACGCATACGACGCCGGCA TCATGCAGCTGAAA-3¢; truncate 6, 5¢-GGGAATTCC ATATGGCATACGACGCCGGCATCATGCAGCTGAA ... ATATGGCATACGACGCCGGCATCATGCAGCTGAA A- 3¢; truncate 7, 5¢-GGGAATTCCATATGTACGACGC CGGCATCATGCAGCTGAAA-3¢; truncate 8, 5¢-GGGA ATTCCATATGGACGCCGGCATCATGCAGCTGAA A- 3¢; truncate 9, 5¢-GGGAATTCCATATGGCCGGCAT CATGCAGCTGAAAGGCAAG-3¢;...
... that isthe most typical mean of indicating language as well as indicating any change of language and life Because language isa mean of human interaction and people are social beings in a society ... local or regional or national or international level The main conveyor of news is newspaper Neither the advent of television nor that of internet could affect the importance ofthe newspaper The ... taken into consideration analysis, transferring, restructuring and testing Analysis consists of essentially of determining the meaning ofthe whole story In other words, all the content of the...
... impart news by the language And it is newspaper that isthe most typical mean of indicating language as well as indicating any change of language and life Because language isa mean of human interaction ... regional or national or international level The main conveyor of news is newspaper Neither the advent of television nor that of internet could affect the importance ofthe newspaper The reason for ... and in the second one Such a sentence can exist only when each half of it is an independent unit of information Under that circumstance, the reader needs not to read this half to understand the...
... probably be classed under the head I am now considering One of his relations has sent me the following account of his early and sudden decease : " He was found dead in his bed ona Monday morning ... effects of training and Boat-racing on their health I have endeavoured to discuss this much vexed question in an honest and impartial spirit I entered onthe enquiry without any personal bias in the ... board the " London" in the Bay of Biscay, another was drowned in swimming a young horse across a river in Australia, another was murdered by poachers, and another accidentally shot Turning from these...
... banking organizations and undermine existing controls For example, unbalanced incentive compensation arrangements can place substantial strain onthe risk-management and internal control functions ... risk-management processes and internal controls to reinforce and support the development and maintenance of balanced incentive compensation arrangements Risk-management and control personnel are engaged ... functions of even well-managed organizations Therefore, risk-management and internal control functions should be involved in designing, implementing, and evaluating incentive compensation arrangements...
... using IMAGEQUANT software, and depurination was calculated by relating the amounts ofthe small aniline-fragment and 5.8S rRNA and expressing values as a percentage Reassociation and quantification ... electrophoresed on an agarose/formamide gel rRNAs were quantified from digital images using IMAGEQUANT software The depurination was calculated by relating the amounts of small aniline-fragment and 5.8S rRNA ... Expression and purification of ricin A chain variants A single colony of Escherichia coli JM101 transformed with the pUTA vector [14] containing the appropriate RTAvariant sequence was used to inoculate...
... urea solution and then run on SDS ⁄ PAGE Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of both ... contraction [10] TnC interacts with both TnI and TnT The TnC–TnI interaction and changes in the interaction upon Ca2+ binding to TnC have been intensively studied as the central mechanisms of ... regulatory TnCbinding site to the C -terminus of Akazara scallop TnI is not important for this regulation, and that Akazara scallop troponin acts through mechanisms in which the region spanning...
... TGGACGGATATTGAAGCTGATCTCACCATAAAGGAT ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC ... material is available online: Fig S1 Sequence alignment ofthe C-terminal regions of Vps4, spastin, katanin and fidgetin from a range of species This material is available as part ofthe online article ... by the addition of Vta1p and a Vps4p protein containing an intact C-terminal helix Based on our experimental data and bioinformatic analysis, we propose a model forthe role ofthe C-terminal...
... ClpA consists of three domains: an N- domain and two ATP -binding domains referred to as the D1 and D2 domains Interestingly, deletion ofthe N- domain from ClpA not only abolishes bindingofthe adaptor ... functional interaction between ClpS and the N- domain of RR ⁄ AA, and this result suggests that neither the local nor the overall structure ofthe RR ⁄ AA mutant was compromised Mutation oftheconserved ... standard, and refer to the protomer Unfolding and protein degradation assays GFP–ssrA degradation was monitored by changes in fluorescence (excitation at 400 nm and emission at 510 nm) Degradation...
... their activity upon incubation, may be responsible forthe only moderate acceleration of fibril formation of N- WT by seeds Consequently, an increase in the protein concentration and ⁄ or temperature ... filtration as published [26] Purified protein Fibrils were formed by incubation of N- (+7)Ala at a concentration of mm for 30 days and N- WT at a concentration of mm for 100 days When fibril formation ... simultaneous presence of both amorphous aggregates and fibrils hampered the analysis of fibril formation kinetics For this reason, fibril formation was analyzed with the N- terminal domain of PABPN1 consisting...
... preparation Patient management Patient education and counselling are integral parts ofthe BoNT -A treatment It is essential forthe patients to have a realistic expectation ofthe treatment outcome, ... results As there are only a few clinical studies and regional guidelines available onthe offlabel indications,12–18 international consensus recommendations would be helpful in providing a general ... solution to obtain a final concentration of 200 s U ⁄ mL (10 s U ⁄ 0.05 mL) This was the concentration used in the majority of international clinical studies forthe treatment of glabellar lines...
... onthe lateral part ofthe nose In some patients, the wrinkles also exist onthe dorsal part ofthethe procerus, the nasalis and the depressor septi nasi The nasalis isthe main muscle responsible ... indications,21–28 international consensus recommendations should be helpful in providing a general guideline for efficacious and safe injection of BoNT -A (Speywood Unit) Consensus recommendations on ... periosteum The orientation ofthe injection should be perpendicular, with an angle of about 45° to the nasal bone To slightly raise the nasal tip, one injection at the base ofthe columella is recommended...
... nucleotide binding to and phosphorylation ofthea subunit Another function of AclB was found to be stabilization ofthe enzyme, as AclB prevented the degradation of AclA that was otherwise observed in ... Efcient dissociation ofthe individual subunits ofthe wild type ACL was conrmed in lanes and The individual subunits ofthe H27 3A mutant Fig Phosphorylation and dephosphorylation of Cl-ACL and the ... occurred in the absence of CoA Taking into account the reaction mechanism of mammalian ACL [23], the nal step ofthe reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated...
... Hoang, M.H Hanh / VNU Journal of Science, Mathematics - Physics 23 (2007) 139-142 Discussion and conclusion In the stationary operation of two-mode random microlaser, the variation of laser parameters ... coefficient α1 augments, the mode photon density n1 is increased and the one of mode nis diminished (see Fig 1a) However, when the gain coefficient α augments, the transformation of photon densities ... saturated values of mode photon densities, we vary one of parameters in table and remain invariable all the rest of parameters The obtained results are shown in Section 3 Influences of laser parameters...
... following asymptotically universal bounds: n area(P0 An+2 ) n area(P0 n area(Pqn+1 An+2 ) n area(Pqn+1 area(Qn An+2 ) area(Qn An+2 ) An+2 ) An+2 ) nn area(P0 ∪ P0 ), nn area(Pqn+1 ∪ Pqn+1 ), area(Qn ... boundary ofa drop U of minimal generation It then follows the boundary of U along a nontrivial arc I Finally, it returns along the boundaries of another chain of descendants of U until it reaches ... decomposition to a unique cell of level nofthe second decomposition, with the dilatation depending only onthe (n + 1)-st term an+1 ofthe continued fraction expansion ofthe rotation number θ The...