5 determining whether an element is within the viewport
Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc
... chromatographic and regulatory characteristics [13] The physiological relevance of this observation remains obscure, together with the nature (either genetic or regulative) of the mechanisms generating these ... in the therapy of malignancies and neurological disorders caused by purine dismetabolisms In this article, we report the kinetic characterization of a series of cN-II mutants, designed on the ... Saccharomyces cerevisiae; and Sp, Schizosaccharomyces pombe Only the three common sequence motifs are reported The numbers indicate the distances to the N terminus of each protein and the sizes of the gaps...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... investigated the influence of the ARE and the CRID on the poly(A) status of the human IFN-b mRNA in natural conditions of IFN-b synthesis Materials and methods Reagents All the reagents and enzymes ... RNAse H in the presence of oligo(dT) before the Northern blot analysis with a HA antisense riboprobe As shown in Fig 3A (lanes and 7), the HA-tagged IFN-b transcript underwent significant deadenylation ... mechanism [18–20], other observations deny any coupling between the recruitment of the mRNA into polysomes and its deadenylation/degradation [7] Here, we analysed whether ongoing translation is...
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Guidance for Determining Whether a Poultry Slaughter or Processing Operation is Exempt from Inspection Requirements of the Poultry Products Inspection Act docx
... under sanitary conditions using procedures that produce sound, clean poultry products fit for human food Attachment of this guidance is a list of sanitary standards and procedures These sanitation ... or her own premises or any other person’s premises • Ostrich and other poultry can be custom slaughtered and processed in an official red meat establishment that is subject to the regulatory ... produced under the PGOP Exemption are distributed solely by the manufacturer and only within the State or Territory or the District of Columbia in which the poultry product is produced; The producer/grower...
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Báo cáo y học: Identification of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b ppt
... responsiveness is slightly enhanced rather than lowered (Table 1) Apparently, the first CAGA box is not required for the down-regulation by TGF-b1 of the SeP promoter To define the actual TGF-b-responsive element ... differences between the assessment of the SeP mRNA expression and the measurement of luciferase activity exist For example, whereas in the first case the species assessed is the mRNA (i.e the respective ... factors which ultimately mediate the transcriptional repression and conceal the antigenic determinants of the Smad molecules Numerous interactions between Smads and other signalling molecules have...
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Báo cáo y học: "Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element" ppt
... assay and drafted the manuscript SP carried out the quantitative real-time-PCR analysis and participated in the manuscript YP participated in the design of the study and analysis of the quantitative ... blot analysis with primary antibodies: anti-GAPDH, anti-firefly luciferase protein (anti-Luc) and antiPoly A binding protein (anti-PABP) and the horseradish peroxidase-conjugated secondary antibody ... suggest either that the PRE sub-section 1399-1684 contains a novel inhibitory element or that the PRE subsection 1151-1398 is an important element for the function of the PRE Taken together, the PRE...
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Tài liệu To establish an Office on Women’s Health within the Department of Health and Human Services, and for other purposes pdf
... establish short-range and long-range goals and objectives for all issues of women’s health within the jurisdiction of the Food and Drug Administra- tion, including adequate inclusion of women and analysis ... FOR DISEASE CONTROL AND PREVENTION OFFICE OF WOMEN’S HEALTH ‘‘SEC 310A (a) ESTABLISHMENT.—There is estab- 19 lished within the Office of the Director of the Centers for 20 Disease Control and ... adding at the end the following: 22 23 ‘‘OFFICE OF WOMEN’S HEALTH ‘‘SEC 713 (a) ESTABLISHMENT.—There is estab- 24 lished within the Office of the Administrator of the Health 25 Resources and Services...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... D-galactotriose also disappears To determine whether a small amount of a-L-1,3-arabinofuranosidase was responsible for the disappearance of Fig HPAEC analysis of the hydrolysis of arabinogalactans by GALA ... means that the removal of the L-arabinose residues from the galactomannan oligosaccharides is caused by traces of an enzyme in the b-1,4endogalactanase preparation that is not active against the ... Simon Flitter for the identification and isolation of the galA containing phage clones and Matthew Illsley for the analysis of a-L-1,5-arabinofuranosidase activity in the endogalactanase preparation...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... or whether it transiently associates and then dissociates In general, the total amount of immunoreactive protein obtained from the human clone was lower than from the murine clone (Fig 1) This ... blot analysis and quantitated as described in Materials and methods Open and filled circles indicate the activity, and open and filled bars the immunoreactivity (PD, pixel density) for human and ... domain This is the first evidence that this region is involved in the formation of the active complex MATERIALS AND METHODS transfected with the pcDNA3 vector alone were used as controls The expression...
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To establish an Office on Women’s Health within the Department of Health and Human Services, and for other purposes ppt
... ADMINISTRATION. The term ‘Administra- tion’ means the Health Resources and Services Ad- ministration ‘‘(2) ADMINISTRATOR. The term ‘Adminis- trator’ means the Administrator of the Health Re- sources and ... ‘‘(2) establish short-range and long-range goals 13 and objectives within the Administration for issues 14 of particular concern to women’s health within the 15 jurisdiction of the Administration, ... establish short-range and long-range goals and objectives within the Health Resources and Services Administration for women’s health and, as relevant and appropriate, coordinate with other ap-...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... further used either to analyze the MS ⁄ MS spectra acquired in the data-dependent acquisition analysis or to build an inclusion list with the light and heavy precursor masses for cross-linked candidate ... using nanoLC-LTQ-Orbitrap MS ⁄ MS analysis in the LTQ The sequence of the loop-linked peptide V340FGGSTRIPK*VQK*L352 is presented with the identified y and b fragment ions Black and grey stars and ... Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14,...
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Autobiography and Letters of Orville Dewey, by Orville Dewey1Autobiography and Letters of Orville Dewey, by Orville DeweyThe Project Gutenberg EBook of Autobiography and Letters of Orville Dewey, D.D., by Orville Dewey This eBook is for the use of an pptx
... was the first clergyman that had ever been a member of it It consisted of artists and other gentlemen, [86] an equal number of each Cole and Durand and Ingham and Inman and Chapman and Bryant and ... for historical Christianity The revelation in our nature, then, and the revelation in the Scriptures; the proper place of each in any just system of thought and theology; what importance is to ... be an improvement upon all existing ones, Roman Catholic, Church of England, or any other If in the highest ranges of human attainment there is to be an advancement of age beyond age, surely there...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... procedures), and the DdesA mutation was verified in one of the mutants by PCR and Southern blot analysis A 1462-bp PCR band corresponding to the extended resistance cassette was found only in the mutant ... pUZ8002 bearing the cosmid with oriT and the S coelicolor DdesA mutant, single crossover exconjugants were screened for kanamycin resistance Kanamycin-resistant colonies were isolated and analysed by ... by desA and its possible involvement in desferrioxamine biosynthesis, two apramycin-resistant and kanamycin-sensitive transformants were isolated among S coelicolor transformants with the desA...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... operon This poses the obvious questions of whether the heterooligomeric c ring can promote ATP synthesis, whether the c subunit stoichiometry is variable, and, if so, whether a variation of the c1 ... ATP synthesis, the following experiments were performed In a fully coupled system, ATP hydrolysis is accompanied by ion transport into the proteoliposomes, and the membrane potential established ... (1981) The mechanism and regulation of ATP synthesis by F1-ATPases Annu Rev Biochem 50, 681–714 Fillingame RH (1992) H+ transport and coupling by the F0 sector of the ATP synthase ) insights into the...
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Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
... Japan) for the technical assistance This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and from Japan ... mcrA1, mcrB1] and plasmids pKT25kan and pUT18Camp were provided by D Ladant (Pasteur Institute, Paris, France) and L Selig (Hybrigenics, S.A., Paris, France) An improved version of the bacterial ... required for the interaction with the middle domain, which mediates the dimerization of HSP90, and the other, the identification of the minimal region of the C-terminal domain for the client binding...
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5 bí quyết ăn chay vừa khoa học lại có thể giảm cân potx
... hàng ngày Uống nước giúp bạn đỡ khát mà tạo điều kiện tiêu hóa thức ăn tốt thúc đẩy hoạt động quan thể, đồng thời còngiảm lượng mỡ tích tụ Nếu bạn muốn uống nước hoa quả, pha thêm nước lọc để...
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THE KINGDOM OF GOD IS WITHIN YOU pptx
... that the meaning of the Christian doctrine has grown continually more and more clear to men The Christian view of life has exerted an influence on the Jewish and heathen, and the heathen and Jewish ... can be distorted into an antichristian and heathen meaning they regard as the foundation of Christianity In support of their assertion that Christianity is not opposed to the use of force, these ... them in the Sermon on the Mount? And can a Christian, then, or can he not, always remaining a Christian, go to law or make any use of the law, or seek his own protection in the law? And can the...
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Báo cáo khoa học: MBP-1 is efficiently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover doc
... Collectively, these data confirm the existence of this transcript in the cells Distribution of the MBP-1 transcript in normal tissues and cancers To determine the distribution of this putative MBP-1 transcript ... alternative transcriptional initiation of ENO1 and is transcribed from the intron III of the gene The transcript can be exported to the cytoplasm, and can be translated in vitro Susceptibility of the MBP-1 ... the ENO1 transcript, and the other is transcribed from an alternative transcription start site in the ENO1 gene locus In a comparison of their relative mRNA abundance in the same cell, the RT-qPCR...
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Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential doc
... extract and 20 lgÆmL)1 gentamicin (Centafarm Services, EttenLeur, the Netherlands) The human small cell lung cancer (GLC4) cell line and GLC4 cell line transfected the human multidrug resistance ... NIM811 and PSC833 induce an increase in cytosolic [Ca2+] and production of ROS The discrepancy in the effects of PSC833 between GLC4 cells and isolated mitochondria versus the consistency in the ... P-glycoprotein is located at the plasma membrane, and is expressed in almost every cell type, including human kidney and neuroblastoma cells [28–30] This pump is able to excrete many different...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008