... might modulate the expressionof ARE -containing genes Accordingly, we tested the effect of overexpressing ASF ⁄ SF2 on the expressionof Renilla luciferase (Rluc) reporter genes carrying (or ... control ofgeneexpression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expressionof ... context of the wild-type ASF ⁄ SF2 protein, both RRMs play equally important roles Overexpression of ASF/SF2 down-regulates the expressionof an ARE -containing reporter mRNA The association of ASF...
... expressionof biosynthetic genes (regulatory genes) [4,9–11] However, the coverage ofgeneexpression profile by cDNA-differential display seems still incomplete, given the lack of a few genes in ... Differential geneexpression analysis of the two forms by cDNAdifferential display led to the identification of several genes involved in biosynthetic reactions (structural genes) and regulation of the expression ... the list ofgenes expressing in a chemovariety-specific manner In addition to anthocyanin biosynthetic genes, red perilla expressed a set ofgenes activated by light, such as ATP synthase of photophosphorylation,...
... constituent of membrane, we examined whether glutamine regulates the expressionofgenes involved in cholesterol homeostasis To investigate the effect of glutamine on the transcription ofgenes related ... in the induction of SREBP-1a geneexpression When endogenous Sp1 expression was reduced to 20% of normal with gene- specific small interfering RNA (siRNA), the geneexpressionof SREBP-1a was ... Induction of SREBP geneexpression is not the initial trigger for the glutamine effects If induction of the SREBP geneexpression serves as the initial trigger for the glutamine effects, the gene expression...
... also showed higher expressionof the molecules involved in angiogenesis, such as VEGF, FLT1, NRP1, NRP2, ANGPT2, JAG1, ARTS1, TNFRSF12A Also the geneexpressionof a group of insulin-like growth ... Israel MA: Geneexpression profiling reveals molecularly and clinically distinct subtypes of glioblastoma multiforme Proceedings of the National Academy of Sciences of the United States of America ... TGFβresponsive gene set, 2708 unique genes were represented by 7173 array elements in the Freije study [25] Among the 7173 probesets representing the TGFβ-responsive genes, 417 representing 323 unique genes...
... possible relevance to pathogenesis J Virol 2005, 79:7819-26 Reghunathan R, Jayapal M, Hsu LY, Chng HH, Tai D, Leung BP, Melendez AJ: Expression profile of immune response genes in patients with Severe ... Interferon-stimulated genes Interferon-stimulated genes RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RT-PCR for the presence of ISG56 and MxA mRNAs ... efficient dissemination of SARS-CoV By slowing down expressionof IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order...
... allows identification of the genes within clusters by visual inspection and subsequent functional analysis ofgenes within clusters (Figures 3A &3B) Figure 3A represents 110 genesof the same cluster ... content mosaic for genes selected as HV -genes in cord blood and adult monocytes, belonging to five clusters of highest Correlative Correlative mosaic for genes selected as HV -genes in cord blood ... collectively highlight the importance of examining geneexpression profiles (or related protein expression levels) over time The limits ofgeneexpression profiling as a technique, albeit a very...
... PRJNA72155 Gene sequences for genes quantified are available through accession numbers TSA JO840460-JO840468 Quantitative geneexpression analysis Nine genes were selected for geneexpression ... Quantitative geneexpression analysis Expression analyses of nine genes were conducted at 0, 3, 6, 12, 24 and 48 hours post infection (hpi) to validate if the pyrosequencing data reflected their geneexpression ... 18S and actin reference genes to give the relative geneexpression The expression data was then compared against the pyrosequencing data which revealed that six of the nine genes showed similarities...
... the production of somatic embryos [69,71] Our data strongly indicate a role for these PRs during coffee embryogenesis Resistance Genes Most of the disease resistance genes (R genes) in plants ... Annotation of 20 genes with the highest expression among Coffea spp cDNA libraries Word file containing the ranking ofgenes more expressed in coffee EST libraries ID: Contig number; #: number of libraries ... 7) The use of regulatory elements of these highly expressed genes may be an excellent tool for conferring strong expression to a target gene in transgenesis approaches To identify genes uniquely...
... levels of the expressionof a specific gene is a true characteristic of some genes, which must be considered in a description of how elevated expressionof a particular gene contributes to pathogenesis ... regulators of Hsa21 genes in man and in mouse is essential to elucidating the genetic basis of the variation ofgeneexpression and its contribution to pathogenesis in DS reports Three genes from ... directly or indirectly regulated by other mmu21 genes Stratification of trisomic genes based on brain geneexpression profiles reports Three trisomic genes that were previously reported not to conform...
... relative to the total number of detected genes Hence, controlling the FDR may be a reasonable strategy The non-Normality of gene- expression data hampers the ranking of the gene- expression effects for ... heterogeneity of variance did not seem to improve FDRe, possibly because of the small sizes of DATA1 and DATA2 which results in poor estimates of the variances of the individual genes In general, ... Efron et al [5] used the 90th percentile of the standard errors of all the genes, i.e., for 90% of the genes, a is larger than the usual denominator of the t-statistic ( s2 /n1 + s2 /n2 ) Although,...
... using Cell Quest software (BD) Results Geneexpression changes were identified by comparing geneexpression profiles across the indicated age groups to the geneexpression profiles of the month age ... ofgenes warranting further scrutiny In order to obtain an expression profile of the genes which changed the most with age, we uploaded all of our array data into the array analysis program of ... 100 changed genes Red represents up-regulation ofgene expression, and green represents down regulation ofgeneexpression http://www.medsci.org Int J Med Sci 2009, 56 Table Top genes that increased...
... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR A good way of optimizing the first round PCR is to take advantage of known genes ... Labeling of a PCR-amplified probe with DIG as part of in situ RT-PCR indirect detection ofgeneexpressionexpression standard RT-PCR was performed showing specific amplification of the TNF gene For ... intensity of green versus red This gives a measure of the relative levels of the competing mRNAs bound to each spot and so provides information on the relative level ofexpressionof the genes on...
... the expression profiling of the 19 genes using four validation cell lines (RERF-LC-MS [AD], LC1/sq [SQ], 86-2 [LC], and MS-1-L [SC]) and the normal control (MRC-9) The results ofexpression profiling ... interaction Figure Gene networks and pathways of 11 genes from PCA analysis The network was analyzed using Ingenuity Pathways Analysis software and is displayed graphically as nodes (genes /gene products) ... microarray database (accession CBX 100) Quantification of 19 candidate genes by qPCR Using qPCR, we quantified the expressionof the 19 candidate genes in the same eight test cell lines (A549, ABC-1,...
... and binds to and regulates basal expression via HSEs of target genes [26] Deletion of the HSF gene is lethal to cells, even at physiological temperatures The genes targeted by HSF encode proteins ... shock and may activate nonclassical heat shock genes, but does not activate classical heat shock genes (i.e HSP genes) HSF1 prefers long arrays of the nGAAn unit, while HSF2 prefers short arrays ... disruption of nucleosomes along the coding region of HSP genes Unlike yeast HSP genes, the DNase I hypersensitive region of the hsp70 promoter of nonheat-shocked cells contains paused Pol II,...
... Hsp105b-induced expressionof the hsp70 gene Nuclear localization of Hsp105b is necessary but not sufficient for activation of the hsp70 promoter The promoter region between )206 and )187 bp of the hsp70 gene ... < 0.01 Downregulation of Hsp105 reduces the induction of Hsp70 expression during heat shock To elucidate the physiological role of Hsp105b-mediated regulation of Hsp70 expression, we examined ... Schematic representation of a series of C-terminal deletion mutants of Hsp105b The asterisk in the Hsp105bmNLS mutant indicates the location of the mutated NLS sequence (B) The expression plasmids...
... Decma-induced Erk phosphorylation Moreover, expressionof silent mutants of Shc isoforms showed that only p46Shc and p52Shc rescued the effect of the siRNA Overexpression of p66Shc not only failed to rescue ... cell–cell contact regulates the expressionof MMPs and uPA in vitro [13–15] However, the underlying molecular mechanisms are not yet fully understood Expressionofgenes for these proteolytic enzymes ... increased expressionof uPA is directly related to higher tumor growth and metastasis [1] Several analyses have already made it clear that the expressionof E-cadherin and the expressionof MMPs...
... mutant for ODC and ADC genes, we used these polyamine auxotrophic parasites as recipients of foreign ODC or ADC genes to study their expression and the eventual suppression of polyamine auxotrophy ... promoter region of pRIBOTEX and related vectors induces the transcription ofgenes cloned downstream of the promoter and their chromosomal integration [19] After 48 h of transfection, geneticin (G418) ... the cultures of transformed parasites to select plasmidcontaining protozoa The time course of ADC geneexpression was followed by northern hybridization analysis and measurements of the new enzymatic...
... pathways Inhibitory effects of n-3 PUFAs on cyclin D1 geneexpression and inhibition of IL-1b-induced Egr-1 gene activation Egr-1 is the product of an immediate-early gene that regulates SMC proliferation ... production of COX-2 mRNA expression [18] PUFAs modulate SMC activation similarly for endothelial cells (i.e proliferation, synthesis of adhesion molecules and expressionof inflammatory genes) when ... the production of PGE2 [18] The inhibition of PGE2 release was the consequence of a reduction of COX-2 and secretory PLA2 mRNA expression Moreover, the IL-1b-induced expressionof secretory PLA2...
... regulates c-myc geneexpression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expressionof a cell growth-related gene, we assessed c-myc geneexpression in ... between the inhibitory effect of NAMI-A on ERK1/2 activation and NAMI-A- induced down regulation of c-myc geneexpression NAMI-A inhibits c-myc gene transcription and protein expression To establish ... stained gels of the reaction products obtained using lL of the RT products after 30 cycles of PCR amplification The lower part of panels A, B and panels C, D report the expressionof c-myc mRNA...
... single or multiple gene predictors not involved ‡ in one of the geneexpression profile tests of interest: 150 Article does not involve one of the three geneexpression tests of interest: 659 No ... confirmed for expression measurements of individual genesof the 70 -gene expression profile, as well as on the 182 most highly expressed genes In the second phase of the study, the assay performance ... between the investigated geneexpression signatures (see Glossary, Appendix B) and the actual gene expressionbased tests The gene “signature” is the collection ofgenes whose expression levels are...