3 molecular biochemistry and physiology of pediatric macular degenerations
Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... or transport of ER proteins, or regulation of ER calcium channel activity [9–12] In plants, a set of 22 orthologs of known PDI family proteins was discovered by a genome-wide search of Arabidopsis ... Approximately 0.4 mg of protein was treated with 10 mU of glycosidase F (Sigma-Aldrich Inc.) at 37 °C for 16 h For crosslinking of proteins, slices of cotyledons were homogenized with 20 strokes of a Dounce ... folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer Biochemistry 30, 613–619 43 Morjana NA & Gilbert HF (1991) Effect of protein...
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Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt
... preparation and the crystal of complex II Lane 1, crystals of complex II Lane 2, molecular weight markers Lane 3, crystallization preparation of complex II The four subunits of complex II are labeled ... the requirements of structure determination, we had to measure the gene sequences and full amino acid sequences of the porcine complex II subunits An abundance of gene sequences of mammalian complex ... purified preparation of complex II could not be crystallized Instead of further purification (e.g gel filtration), the mixture was crystallized directly It may Fig SDS-PAGE analysis of the crystallization...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... Nucleotide sequences of the ORF of both PDI genes were exactly the same as those of cDNAs cloned in this study Comparisons of soybean genomic sequences of GmPDIL-1 or GmPDIL-2 with those of Arabidopsis ... CTNAN [34] In the case of GmPDIL-2, 10 of the 11 introns had TTNAN, and the eighth intron possessed CTNAN Promoter regions of around kb and 1.5 kb upstream of each start codon of GmPDIL-1 and GmPDIL-2 ... protein, were detected at a density of 1.21 gÆmL)1 in the presence of MgCl2 In the presence of EDTA, which causes release of ribosomes from the rough ER, the peaks of GmPDIL-1, GmPDIL-2 and BiP displayed...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... structures of isoquinoline alkaloids, because the structures of many P450s are closely correlated with those of their substrates Further identification and characterization of other members of the ... concentration of 0.4 lm (Fig 3) Expression of CYP719A2 and CYP719A3 genes in E californica plants: tissue specificity and their response to methyl jasmonate (MeJA) We examined the accumulation of mRNA of ... is a traditional medicinal plant of Native Americans, and has been well investigated because of the variety and pharmacological effects of its alkaloids One of the alkaloids produced by this...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... Luffa cells (A) Time course of accumulation of bryonolic acid and total sterol in cultured Luffa cells Mean of three replicates Bar indicates SD (B) Time course of levels of isomultiflorenol synthase ... with those of authentic isomultiflorenol As the control pYES2 transformant produced no triterpene mono-alcohol [3], accumulation of Fig Multiple alignment of deduced amino-acid sequences of isomultiflorenol ... product of LcIMS1 isomultiflorenol synthase, could not be identified Cloning of isomultiflorenol synthase (Eur J Biochem 268) 6315 Fig Time course of bryonolic acid accumulation and levels of isomultiflorenol...
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Morphology and physiology of viruses
... single-stranded or doublestranded; strategy of replication (2) Size and morphology, including type of symmetry, number of capsomeres, and presence of membranes (3) Presence of specific enzymes, particularly ... opposite vertices define axes of fivefold rotational symmetry: all structural features of the polyhedron repeat five times within each 360° of rotation about any of the fivefold axes Combined ... replication of adenovirus, which contains DNA in its genome Replication of poliovirus, which containing an RNA genome Measuring the Size of Viruses A Filtration Through Collodion Membranes of Graded...
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Viruses STRUCTURE, CLASSIFICATION AND PHYSIOLOGY OF VIRUSES
... consist of a core of nucleic acid, either DNA or RNA, and a protective coat of protein molecules and sometimes lipids In isolation, viruses and bacteriophages show none of the expected signs of life ... After many years of a constant battle, the body has insufficient numbers of T-Cells to mount an immune response against infections At the point when the body is unable to fight off infections, ... not any of the things we normally associate with life Strictly speaking, they should not be considered "living" organisms at all However, they are more complex than a lifeless collection of macromolecules...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... regions of the mature peptide To obtain the CHH cDNA, we used 10 lg of total eyestalk RNA from adult shrimp by RT-PCR assays The quality of synthesized cDNA was confirmed by PCR amplification of L ... residues at the same positions as that of other CHHs of penaeid shrimps (Fig 1B) Tissue-specific gene expression of L schmitti CHH gene The size and expression of the CHH mRNA in different tissues ... 5671 Functional analysis of CHH by RNAi Glucose (mg/dL) 20 16 J M Lugo et al 0h 24 h 12 Saline Unrelated dsRNA CHH dsRNA Fig Effects of injection of dsRNA in the profile of the hemolymph glucose...
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Báo cáo Y học: The sodium pump Its molecular properties and mechanics of ion transport potx
... moiety of the ATP molecule, but that it is merely within reach of the highly reactive azido group of 8-azido-ATP In the crystal structure of the Ca2+-ATPase, Lys492, the equivalent of Lys480 of the ... membrane peptides and also offers the possibility of addressing physiologically relevant questions in connection with the regulation of the expression of this type of protein THE CATALYTIC MECHANISM ... or K+ ions A number of isoforms of the a and b subunits has been isolated from various tissues of numerous species, and it has been repeatedly demonstrated that the function of Na+/ K+-ATPase requires...
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kumar - molecular genetics and breeding of forest trees (haworth, 2004)
... Modification of PAL and C4H Genetic Modification of 4CL Genetic Modification of C3H Genetic Modification of CCoAOMT Genetic Modification of CCR Genetic Modification of F5H Genetic Modification of COMT ... of wood and tree production, with the goal of enabling new kinds of highly domesticated, biosafe plantations to be employed Highlights of the book include a description of the EST databases of ... 141 142 143 The Site of Cellulose Biosynthesis Current Knowledge of the General Process of Cellulose Synthesis in Plants Identification of CesA Genes in Plants Isolation of New CesA and CSLD...
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báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt
... unpaired data mode of the algorithm As a cut-off for significance, an estimated FDR of 2.6% was chosen by the tuning parameter delta of the software The significance level of each gene was given ... genes of a sample, queried their expression values and made the decision of "part of the class" or "outside the class" For each classifier and LOOCV iteration, the frequency of a gene (how often ... each of the four classes were chosen in order to generate a final classifier of 40 genes The accuracy of correct classification of the tissue is calculated as percentage using 30 classifiers of...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... 59.5% of the radioactivity was bound The addition of 10 μg of unlabeled fragment, which reduced the percentage of bound radioactivity to an average of 3.5%, demonstrated the specificity of the ... comprised of a single product consistent with the Mr of a F(ab’) (data not shown) The final yield of this preparation was appreciably higher than the digestion of 100 mg with a final yield of 56% ... those of the F(ab’)2 fragment with a higher overall detection of tumor lesions The authors postulate that the success of these studies was the result of careful selection and matching of the...
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Báo cáo khoa học: "Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea" potx
... *Map position of the primers based on the published sequence of Western buxton (AF078669) Results Table Kinds of potential motif exist in VP1 of GC1 Nucleotide and amino acid sequences of the VP1 ... were compared with the corresponding sequences of previously reported cite accession numbers of aquabirnaviruses in Table Genogrouping of VP1 of aquatic birnavirus 87 Table RT-PCR primer sets ... Descriptions of VP1 sequences of aquabirnaviruses cited in this study Name of virus GC1 578 1146 2290 AM98 AY98 DRT Jasper NC1 Sp WB Y6 YT01A CLV 88R 24R 20Gld 19F3b 6B1a H1 UPM97/61 DXV Genus of virus...
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Báo cáo lâm nghiệp:"Performance and physiology of large containerized and bare-root spruce seedlings in relation to scarification and competition in Québec (Canada)" pot
... 0.947 0.063 age of 34% of full sunlight while they received 51% of full sunlight at LC At both sites, soil scarification did not significantly affect vegetation density of any of the vegetation ... nature of the specific scarification technique, which can create an array of microsite conditions even within a single type of treatment [57] 4.2 Impacts of competing vegetation The presence of competing ... case of significant correlation, we used ANOVA for completely rand- We tested all data for normality and homoscedasticity of the experimental errors In the case of departure from normality or of...
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Báo cáo khoa học: " Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon" ppsx
... amplification of cDNA ends (RACE) kits (Invitrogen) as recommended by the manufacturer Nucleotide sequence alignment of SAV3 UTRs with those of SAV1 and SAV2 (sequences of the UTRs of SAV4-6 subtypes ... replaced with that of the gene of interest (GOI) Expression of the GOI is then executed by the alphavirus replication apparatus Such replicons are frequently used for basic studies of alphavirus replication ... characterization of SAV3 5'- and 3'-ends The 5'- and 3'-ends of SAV3 were cloned and sequenced from the isolate SAVH20/03 and aligned to the 5'- and 3'-UTRs of other SAVs A) Alignment of the region...
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A PROSPECTIVE STUDY ON DETECTION, SUBTYPE ANALYSIS, CHARACTERIZATION, MOLECULAR EPIDEMIOLOGY AND TRANSMISSION OF INFLUENZA VIRUSES AMONG UNIVERSITY STUDENTS AND STAFF IN SINGAPORE
... TABLE OF CONTENTS Page CONTENTS Declaration page I Acknowledgements II Table of contents III Publications, presentations, awards VII Summary VIII List of tables XI List of figures XIV List of abbreviations ... 1.2 Important determinants of influenza virus pathogenicity Table 1.3 The Origin of Swine Influenza Virus Segments 11 Table 1.4 Summary of characteristics of pandemics of 20th and 21st century ... B 35 Table 2.8 List of primers for sequencing of surface genes of influenza A virus 37 Table 3.1 Demographic characteristics of study population 42 Table 3.2 Viral etiology of ILI on NUS campus...
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Molecular biology and pathogenesis of coronavirus virus and host factors involved in viral RNA transcription
... research ii Table of Contents Summary vii List of Tables ix List of Figures x List of Publications Chapter Literature Review: The Biology of Coronavirus xvi 1.1 Overview of Coronaviruses ... Table 2.3: List of primer sequences used in the cloning of all full-length and truncated MADP1 constructs 71 Table 2.4: List of all primer sequences used in the generation of mutants of pXJ40Flag-MADP1 ... densities of MADP1 (normalized with band densities of actin for each cell line) in 16 cell lines classified by tissue of origin 163 ix List of Figures Figure 1.1: Schematic diagram of a coronavirus...
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Molecular transport and structure of DNA in a congested state
... the method of multiple-particle tracking to measure the microviscosity and degree of heterogeneity of solutions of DNA over a wide range of concentration and lengths to identify some of the topological ... Physical Review E, 81: 061905 (2010) ii Table of Contents Acknowledgement i List of Publications ii Table of Contents iii Summary vi List of Tables vii List of Figures viii Chapter Introduction 1.1 ... supports DNA is of paramount importance (9, 10) The ionic strength of the supporting medium provides different screening conditions of DNA From a biophysical point of view, it is of interest to...
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Molecular function and regulation of the bax associating protein MOAP 1
... capable of dramatically extending the half-life of MOAP-1 and promote the accumulation of poly-ubiquitinated forms of MOAP-1 in a variety of mammalian cell lines Interestingly, induction of MOAP-1 ... i TABLES OF CONTENTS SUMMARY ABBREVIATION LIST OF FIGURES LIST OF TABLES INTRODUCTION 1.1 APOPTOSIS 1.1.1 Definition and morphology of apoptosis 1.1.2 Extrinsic and intrinsic pathways of apoptosis ... characterized by shrinkage of the cell, hypercondensation of chromatin, cleavage of chromosomes into nucleosomes, violent blebbing of the plasma membrane without rupture and packaging of cellular contents...
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Molecular cloning and characterization of sq163, a zebrafish liver mutant
... hypoglycemia and failure of expression of genes associated with hepatocyte activities Disruption of the formation of bile canaliculi, a consequence of the failure of hepatocyte expression of cell junction ... of genomic DNA pooling in BSA 83 Figure 2-5 84 Figure 3-1 Schematic illustration of the experimental layout and output of rough mapping Scheme of genetic screening 111 Figure 3-2 Phenotypes of ... epithelial lining of the whole of the digestive tube except part of the mouth, pharynx and the terminal part of the rectum Expectedly, it also contributes to the cell linings of all the glands...
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