2 features of musculoskeletal tissues which impact tissue engineering
liver stem cells methods and protocols
... buffers (4C) Harvesting of the hepatic tissue from mouse fetuses (E 14.5) Enzymatic dissociation of the tissues Filtration (60 àm) Decantation step (15 min) Isolation of mouse fetal liver cells ... Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore MARI DEZAWA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, ... portal vasculature if repeated transfusions of large number of cells are required, further increasing the risks of bleeding, infection, and loss of viability of transplanted cells After infusion into...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... Downregulation of HIF-1a decreased the enhanced PI of NPCs induced by hypoxia (A) Cytometry analysis indicates that the PI of NPCs was decreased by downregulation of HIF-1a (B) Graph of the PI of NPCs ... growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells [12] Therefore, ... that hypoxia induces the transcription of HIF-1a mRNA, which increases the level of HIF-1a protein in the presence of continued hypoxia [37,38] Expression of HIF-1 (protein level) was markedly...
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báo cáo hóa học:" Circulating endothelial progenitor cells: a new approach to anti-aging medicine?" docx
... tissue insult is acute respiratory distress syndrome (ARDS), in which respiratory failure often occurs as a result of disruption of the alveolarcapillary membrane, which causes accumulation of ... cells, it was found that areas of the vasculature with high endothelial turnover, which were the areas of elevated levels of sheer stress, had incorporated the majority of new endothelial cells derived ... key component of endothelial turnover appears to be the existence of circulating endothelial progenitor (EPC) cells that appear to be involved in repair and angiogenesis of ischemic tissues An early...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... plated at a concentration of 20000 cells per well in 96 well plates After days of culture, level of cellular ATP was quantified by bio-luminescence The ratio of average values of ATP in growth factor ... existence of various subsets of cells with EPC potential residing outside of the CD34+ KDR+ fraction Further studies are required to elucidate functional importance of the variable kinetics of mobilization, ... composed of a combination of blueberries, green tea extract, carnosine, and vitamin D3 In vitro activity on proliferation of human bone marrow cells was assessed, in which a 60% enhancement of growth...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong to the family of mesenchymal ... increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of MSCs in ... of the hepatic artery, and the presence of any vascular occlusion or changes in the flow patterns We will monitor lung toxicity by assessing the necessity of reintubation and the occurrence of...
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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx
... with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong to the family of mesenchymal ... increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of MSCs in ... of the hepatic artery, and the presence of any vascular occlusion or changes in the flow patterns We will monitor lung toxicity by assessing the necessity of reintubation and the occurrence of...
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Transcoronary Transplantation of Progenitor Cells after Myocardial Infarction ppt
... CPC infusion, which were of only borderline significance It is likely that the smaller number of progenitor cells derived from 270 ml of venous blood, which was 1/10 the number of monocytic cells ... in because of inadequate contrast opacification, in because of ventricular extrasystoles, and in because of the patients’ refusal to undergo invasive follow-up Thus, a total of 72 of 81 serial ... previously was followed by the administration of 7500 to 10,000 U of heparin and (in 89% of the cell-treated patients) a bolus of abciximab (0.25 mg per kilogram of body weight) Cells were infused into...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... up to 70% of individuals, usually with a delayed onset of several months after treatment [1,2] Even if examination of all cancers were to lower the frequency of these problems to 25% of the lower ... great as for many of the more widely recognized neurological syndromes Despite the clear evidence of the neurotoxicity of at least some forms of chemotherapy, studies on the effects of chemotherapeutic ... contribution of clones consisting of just one or two oligodendrocytes and no progenitor cells (with 16 out of 100 such clones seen in control cultures and 42 of 100 in those transiently exposed Journal of...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... terms of the Ethics Committee of the University of Ulm The age of the donors ranged from 55 to 89 years (mean 74 years) The diagnosis was based on clinical and radiological criteria None of the ... recapitulation of development such as expression of procollagen type IIA [29] Possibly, the activation of MPCs may contribute to the observed expression of this alternative splice variant A misguiding of ... combinations of cell surface markers for MPCs offer powerful tools with which to study the biological properties of defined subpopulations and their involvement in disease or repair processes Of course,...
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Báo cáo y học: "It’s all in the blood: circulating endothelial progenitor cells link synovial vascularity with cardiovascular mortality in rheumatoid arthritis" docx
... comparable levels of adhesion to unstimulated endothelial cells or matrix proteins such as laminin [8] What is the relevance of these findings in terms of our understanding of RA? Increased angiogenesis ... supplying nutrients and oxygen to the synovial tissue in RA, which by virtue of its proliferative nature becomes rapidly hypoxic [1] The presence of EPC in RA synovium could result from their ... observations of reduced peripheral blood EPC in RA However, the corollary of augmented synovial tissue EPC paralleled by reduced circulating EPC might also be an impaired ability to revascularise areas of...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... the time of inclusion, 18 of 44 patients had a SLEDAI score of 0, 17 had a score of and had a SLEDAI score of Exclusion criteria were pregnancy, diabetes mellitus, cancer, presence of cardiovascular ... participant The number of patients and controls included per assay are denoted below Because of the low number of CPCs in the circulation of SLE patients and limitations in the amount of blood to be ... maturation of these cells, the expression of CD133 is lost The combined expression of CD34 and CD133, therefore, defines a population of (immature) circulating progenitor cells Because of the low...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... the lower level of expression of particular markers of differentiation could be the result of prior in vivo exposure of T-MPCs to high concentrations of inflammatory cytokines, which are characteristically ... (Figure 3b) (T-MPCs) Surface epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived ... fetal development is invaded by lymphoid tissue (of mesodermal origin) Therefore, embryologically, tonsils could be a source of MPCs Because of the prevalence of tonsillectomy procedure, tonsils are...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... averages of duplicate cultures The use of lentiviral vectors permitted higher levels of gene transfer (>90%) into CD34+ cells with both of the constructs as assayed by EGFP expression Two rounds of ... http://www.aidsrestherapy.com/content/1/1/2 Figure FACS profiles of thymocyte subsets derived in SCID-hu grafts FACS profiles of thymocyte subsets derived in SCID-hu grafts: To determine the presence of different thymocyte subsets, ... improved level of protection is likely due to higher levels of gene transduction and expression, lower levels of transgene silencing during cell differentiation steps, or a combination of both Although...
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Báo cáo y học: "Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells" pot
... regulation of glutamatergic neuronal fate Pax6 induces expression of Ngn2 and NeuroD, which are involved in glutamatergic Page of differentiation and reduces expression of Mash1, which induces ... of Nestin and (B) Tuj-1 was determined by Western blot after ethanol treatment Ethanol (50 mM) decreased the expression of Nestin to 70% of control level and increased that of Tuj-1 to 170% of ... Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0016738) Page of 9 10 11 12 13 14 15 16 Author details Department of Pharmacology, College of Pharmacy,...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... We have recently studied the roles of Sall4 isoforms in controlling murine HSC/HPC properties Our data indicate that a certain level of expression of Sall4 isoforms is necessary for normal HSC/HPC ... indicated that overexpression of Sall4 increased the repopulation activity of HSC/HPCs Of note, however, though HSC/HPCs were expanded by sustained ectopic expression of Sall4 isoforms and exhibited enhanced ... with or without transduction of Sall4 isoforms are all strictly IL-3 dependent, which are unable to grow when removed from IL-3 Thus, over-expression of Sall4 isoforms did not convert the 32D...
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báo cáo khoa học: "Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells" pptx
... shown at 450 nm (i.e., at the edge of the excitation peak which is centred at 458 nm with a FWHM of about 12 nm) in order to be able to show the spatial structure of the sample Strong reflection ... was used Excitation power control was 20% Page of remained loosely attached to the bottom of the dish after washing steps The optical spectrum of one of such QD cluster outside EPCs is shown in ... surrounding Page of the QDs shields the QDs so that the excitation of the QDs inside the cells is usually weaker QDs outside the cells are excited to higher degree, which leads to the saturation of the...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... Formation of type I collagen-producing, alpha smooth muscle actin (aSMA)-positive myofibroblasts is a hallmark of pulmonary fibrosis Despite decades of extensive research, the origin of pulmonary myofibroblasts ... lung tissues differ in their regenerative capability Therefore, we concluded that the microenvironment of injured lung tissue dictates the fate and function of bone-marrow-derived Page 12 of 13 ... one representative out of five independent experiments (A, B) Quantification of flow cytometry analysis of prominin-1 + /CD45+ cells out of all analyzed cells in the lung tissue following bleomycin...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... (University of Konstanz) for their assistance, and Mrs Ina Kähler, Prof M Clauss (University of Indianapolis) and Prof M D Menger (University of Saarland) for the critical reading of the manuscript ... design, aqcuisition of data, analysis of data and interpretation thereof; J Wechselberger performed charcterisation and isolation of EPC; W Hilbe was engaged in evaluation of immunoimmunochemistry ... tolerability of intraveinous application of EBM-2 (1 ml) alone turned out to be safe Intravenous application of EPC was performed 50 to 120 after reperfusion of the transplanted left lung Assessment of...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... preferential sequestration of these cells in inflamed lung tissues To determine homing of BM-derived cells to inflamed lung tissues, we investigated the localization of these cells at 24 hours ... weight) Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias was considered to represent 18.1% of total ... results were shown as percentages of total number of transfused donor cells Histological analysis and detection of donor cells Thin sections (5 µm) of lung tissue were prepared from paraffin-embedded...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... uptake of cell-penetrating dyes [13] or their ability to grow out of cartilage tissue [9] Alternatively, the expression of typical membrane-associated proteins can be employed for the selection of ... the relative percentage of CD166+ chondrocytes by immunofluorescence, analyzing the zonal distribution of these cells within the matrix of normal and OA cartilage was of particular interest Owing ... contributed to acquisition of data, analysis and interpretation of data, and drafting and critical revision of the manuscript SR contributed to acquisition of data and critical revision of the manuscript...
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