1 9 dimethyl methylene blue total sulfated proteoglycan assay
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... 0. 71 1.07 1. 61 19. 22 11 .11 2.55 8.25 2. 61 9. 08 11 .11 11 9 .11 10 4.33 61. 32 39. 23 15 8.48 61. 60 45 .93 18 0.30 23. 81 1 19 .37 92 4 .13 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 1. 00 apolipoprotein ... 0. 81 227 ⁄ 12 4 41 33 ⁄ 12 4 41 59 ⁄ 12 4 41 78 ⁄ 12 4 41 43 ⁄ 12 4 41 326 ⁄ 12 4 41 99 ⁄ 12 4 41 48 ⁄ 12 4 41 5 39 ⁄ 12 4 41 135 ⁄ 12 4 41 15 ⁄ 12 4 41 25 ⁄ 12 4 41 392 ⁄ 12 4 41 330 ⁄ 12 4 41 ⁄ 12 4 41 1.82 ... 12 .04 11 2.80 1. 00 0.03 0. 01 0. 01 0. 01 0. 61 1 .94 1. 23 0.25 5.62 6. 09 23. 89 3.24 1. 00 1. 00 1. 00 1. 00 0.02 0.40 0. 01 0.03 0. 01 0. 01 0 .99 0.66 0.02 0. 51 0.03 0.55 1. 81 24.82 9. 27 0. 71 1.07 1. 61 19. 22...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... cells Circulation 2004, 10 9, 12 92 -12 98 31 Rucker NC, Lumb WV, Scott RJ Combined pharmacologic and surgical treatments for acute spinal cord trauma Am J Vet Res 19 81, 42, 11 38 -11 42 32 Safford KM, Rice ... Res Protoc 2005, 14 , 17 1 -18 0 11 Gimble J, Guilak F Adipose-derived adult stem cells: Transplantation of ASCs in spinal cord injury 283 12 13 14 15 16 17 18 19 20 21 22 23 24 25 isolation, characterization, ... injured spinal cord tissue Spine (Phila Pa 19 76) 2004, 29, 19 71- 197 9 34 Silver J, Miller JH Regeneration beyond the glial scar Nat Rev Neurosci 2004, 5, 14 6 -15 6 35 Song HJ, Stevens CF, Gage FH Neural...
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Investigation of osteogenic characteristics of human adipose derived stromal cells
... t2 20 60 Pa t1 10 10 Pa t1 10 30 Pa t1 50 30 Pa t1 51 00 Pa t1 60 20 Pa 5A t1 60 20 5B Pa t1 60 40 Pa t1 90 10 Pa t1 90 30 Pa t2 50 80 Pa t3 01 10 Pa t2 40 60 Pa t2 90 Pa 04 t0 10 20 5A Pa t2 ... osteogenesis Fig 4 .11 B1 and B2 show the two different isoforms of osteopontin obtained As a control ß-actin (Fig 4 .11 C) and hFOBs cell line (Fig 4 .11 D) were used Fig 5 .1 ATF4 expression pattern ... while CD 16 6 has been postulated to play a significant role in osteogenic differentiation (Bruder SP et al., 19 98 ) The ADSCs uniformly expressed HLA- ABC, CD 90 (Thy -1) , CD 29 (integrin 1) which...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... The authors declare that they have no competing interests 21 Received: 12 December 2 010 Accepted: 19 August 2 011 Published: 19 August 2 011 22 References Barry FP, Murphy JM: Mesenchymal stem cells: ... Science 2 011 , 18 : 59 http://www.jbiomedsci.com/content /18 /1/ 59 fixed in 4% Paraformaldehyde for 20 and stained with Oil red-O solution to show lipid droplets in induced cells [5 ,13 ,15 ] To quantify ... Science 19 99 , 284 :14 3 -14 7 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma Curr Top Microbiol Immunol 2000, 2 51: 3 -11 He X,...
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Characterization of human adipose tissue derived adult multipotent precursor cells
... cells 9 .1 Chapter Abstract 9 -1 9. 2 Introduction 9- 2 9. 3 Specific Materials And Methods 9- 7 9. 4 Results And Discussion 9 -13 9. 5 Conclusion 9- 29 Chapter 10 – Conclusions and Future Work 10 .1 Summary ... (Ferrari G et al., 19 98 ; Gussoni E et al., 19 99 ), liver and epithelium (Petersen BE, et al., 19 99 ; Theise ND et al., 2000), heart (Tomita S et al., 19 99 ; Orlic D et al 20 01, Kucia M et al., 2004) ... known (Owen M 19 88; Bennett JH et al., 19 91 ; Hicok KC et al., 19 98 ; Nuttall ME et al., 19 98 ) A disorder called progressive osseous heteroplasia, where calcified nodules form in 2– 11 Chapter Literature...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... 0.6 21. 5 ± 0.2* 8.6 ± 0 .1 1 79. 5 ± 3.4*** 3.7 ± 0 .1 9. 3 ± 0.2* 6.4 ± 0. 01 31. 4 ± 0.4* 50.6 ± 1. 5* 14 .6 ± 0.3 26 .9 ± 0.4 55.3 ± 1. 1 15 .7 ± 0 .1 31. 0 ± 0.3 9. 0 ± 0.3 498 .5 ± 6 .9 3.3 ± 0.4 11 .3 ± ... 39. 6 ± 3.5 62.7 ± 4.5 13 .9 ± 1. 3 31. 5 ± 0.8 47.2 ± 1. 3 13 .4 ± 0.8 27.6 ± 2 .9 8 .9 ± 0.6 376.5 ± 30 .1 3.2 ± 0.03 10 .6 ± 0.7 5.8 ± 0 .1 39. 6 ± 2.5 55.7 ± 0.4 15 .8 ± 1. 0 28.6 ± 2.2 52.3 ± 2.3 14 .9 ... Marker CD11c CD123 CD1a CD40 ICAM -1 CD62L CD80 (B7 -1) CD83 CD86 (B7-2) DC-SIGN HLA-ABC HLA-DR Mock Empty LV LV MLV 48.8 ± 3.2 13 .0 ± 0.4 27.3 ± 1. 1 8.6 ± 0 .1 462.6 ± 57.5 3.3 ± 0 .1 9. 9 ± 0 .9 5.8...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... (c) C 10 4 (d) D 0. 01 10 0.24 10 2 10 1 10 0 0.43 10 0 10 1 0. 31 103 CD133 CD133 10 3 0.03 10 2 10 1 10 2 10 3 10 0 0. 41 100 10 1 10 4 ZO -1 102 10 3 10 4 ZO-2 (e) E 10 4 0. 01 0.03 10 3 10 2 10 1 10 0 10 0 10 1 10 2 10 3 ... 2008, 9: R83 http://genomebiology.com/2008 /9/ 5/R83 Genome Biology 2008, (a) A Volume 9, Issue 5, Article R83 Birnie et al R83.6 (b) B 10 4 0. 01 0 .16 CD133 10 3 10 2 10 1 10 0 10 0 0.33 10 1 10 2 10 3 10 4 ... IL6 1. 93 5 .18 CASP8 and FADD-like apoptosis regulator CFLAR 1. 90 1. 41 Smu -1 suppressor of mec-8 and unc-52 homolog (C elegans) SMU1 1. 90 1. 63 S100 calcium binding protein A3 S100A3 1. 92 1. 55...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... 99 4.5.3 TGF- 1 and its antagonist A2M regulate the maintenance of renal epithelia 10 1 Discussion 10 5 5 .1 Characterization of isolated and commercial HPTC 10 5 5.2 ... Figure 17 : Immunostaining of phosphorylated Smad1/5/8 in C2C12 cells 86 Figure 18 : Immunostaining of phosphorylated Smad2/3 and phosphorylated Smad1/5/8 in C2C12 cells 87 Figure 19 : ... compounds and for the regulation of the concentrations of ions and homeostasis (1, 6-8, 10 , 15 , 16 , 18 , 19 , 23, 29, 30) In addition, PTC have several important metabolic functions For instance,...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
... Reproduced from (Ikada et al 19 98 ) by permission of Elsevier Copyright © 19 98 , Elsevier 17 Figure 2.3 A Schematic representation of gelatin microsphere fabrication and cell seeding 19 Figure 2.4 22 A schematic ... Introduction 1. 1 Background and motivation 1. 2 Hypothesis 1. 3 Objectives Literature review 2 .1 Tissue engineering and regenerative medicine 2.2 Stem cells in tissue regeneration 2.2 .1 Embryonic ... positively charged basic biomolecules and vice-versa Reproduced from (Ikada et al 19 98 ) by permission of Elsevier Copyright © 19 98 , Elsevier Different types of scaffolds such as hydrogels, nanofibres and...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... 2003, 52: 39- 49 16 Childs JD, Piva SR: Psychometric properties of the functional rating index in patients with low back pain Eur Spine J 2005, 14 :10 08 -10 12 doi :10 .11 86 /17 52 - 19 47-5- 296 Cite this ... Journal of Medical Case Reports 2 011 , 5: 296 http://www.jmedicalcasereports.com/content/5 /1/ 296 Page of Received: 11 October 2 010 Accepted: July 2 011 Published: July 2 011 References Korean Food and ... evaluation 90 15 10 four weeks post-treatment followup 10 0 20 10 12 weeks post-treatment follow-up 10 5 20 15 VAS = visual analog scale Figure MRI of the right hip; T1 sequential coronal views The blue...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... NU-04 69. 41 4.02 SKBR3 -0. 09 13 .53 ** U 118 MG SW 598 51. 96 14 . 29 1. 48 4 .14 MDA-MB-468 GI -10 1 -3.46 -6. 41 0.25 0 .92 A172 2.26 4 .97 SV-BR -1 -8.00 1. 75 IMR-5 -1. 23 3. 09 MDA2 31 -16 . 21 2.60 IMR-32 -3 .16 ... 17 .46 -13 .48 5. 01 11. 21 Caov-3 -14 6.53 2. 69 MCF-7 16 .95 0. 39 ** SW 5 79 68 .97 3. 41 734B 16 .72 2.32 SW 19 49 43 .90 13 .68 T47D 8.47 1. 23 BT-474 0.83 11 .53 Thyroid (1/ 2) Brain (2 /9) Breast (0 /9) ** ... 8 .17 2. 51 ** CAKI-2 SW 15 6 63.62 36. 51 5 .17 10 . 69 * HOS 42.58 4.86 ACHN 9. 85 0.20 * SW 13 53 42.22 4.42 SK-NEP -1 0.00 1. 82 HT 10 80 19 .37 5 .92 SA-4 12 .53 1. 05 ** SW 19 61 64.55 3.04 HS 91 9 3.01...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... declare that they have no competing interests http://www.tbiomed.com/content/2 /1/ 25 10 11 12 13 14 15 16 17 18 19 20 21 22 Authors' contributions ESS, TT, NT, QVN and GDP contributed equally to ... Engl J Med 19 92 , 327 :90 5 -90 9 Munne S, Lee A, Rosenwaks Z, Grifo J, Cohen J: Diagnosis of major chromosome aneuploidies in human preimplantation embryos Hum Reprod 19 93 , 8: 218 5- 2 19 1 Baart EB, ... stem cells Nature 20 01, 414 :92 -97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics J 19 99 , 9 :13 7 -15 0 Coalition urges passage...
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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
... CHAPTER 1. 1 Introduction…………………………………………………… 1 Background………………………………………………………….…………… .1 1 .1. 1 1. 1.2 Pancreas and islet transplantation……………………………………… 1. 1.3 Cell therapy……………………………………………………………… 1. 1.4 ... blastocyst in 19 81 (Evans et al 19 81; Martin 19 81) Professor Ariff Bongso’s group in National University of Singapore was the first to discover stem cells from human preimplantation embryos in 19 94 (Bongso ... Materials………………………………………………………………………… 49 3 .1. 1 Materials and Methods………………………………………… 49 Primary cells and cell lines……………………………………………… 49 vi 3 .1. 1 .1 Cord lining epithelial and mesenchymal cells………………… 49 3 .1. 1.2 Choriocarcinoma...
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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
... Constancia Urbani for secretarial assistance FAPESP/CEPID, CNPq and FUSP References 10 11 12 13 14 15 16 17 18 19 Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans ... 20 09, 31: 9 - 19 Gargett CE, Schwab KE, Zillwood RM, Nguyen HP, Wu D: Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium Biol Reprod 20 09, 80 :11 36 -11 45 ... dental pulp stem cells J Craniofac Surg 2008, 19 (1) :204- 210 Gargett C: Uterine stem cells: what is the evidence? Hum Reprod Update 2007, 13 (1) :87 -10 1 Cervelló I, Martínez-Conejero J, Horcajadas...
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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx
... comparison J Orthop Res 2005, 23 :13 83 -13 89 Taswell C: Limiting dilution assays for the determination of immunocompetent cell frequencies J Immunol 19 81, 12 6 :16 14 -16 19 Tew SR, Li Y, Pothacharoen P, ... pericytes J Exp Med 19 88, 16 7 :10 03 -10 15 41 Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO -1 Blood 19 91 , 78:55-62 42 ... perlecan, collagen type I (COL1A2), collagen type II (COL2A1), collagen type IX (COL9A1), collagen type X (COL10A1), collagen type XI (COL11A2), L-SOX5, SOX6, and SOX9 Total RNA was extracted with...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... 7 .1 0. 31 0. 01 0 .1 Multiplicity of infection (MOI) 40 20 3.56 (16 9) 97 . 69 94.63 10 0 80 60 10 D) 42-7: Infected with MLV MFG EGFP 80 2 .96 10 C) 42-7: Infected with HIV -1 CSII EGFP 10 0 35 .97 29. 7 ... CSII EGFP 10 0 35 .97 29. 7 3.63 (18 1) 73.43 40 20.22 ( 19 7) 0. 01 85 .1 60 45.84 (236) 20 92 .18 80 60 10 0 93 . 29 (840) Parental Mutant 10 38. 59 20.66 2.33 0.55 0. 01 3.78 0 .1 10 Multiplicity of infection ... in eukaryotic cells Gene 19 88, 66 (1) :1- 10 Page 10 of 11 (page number not for citation purposes) Retrovirology 2007, 4:45 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Aiken C:...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... times 15 2× 10 3 4× 10 3 8× 10 3 10 × 10 3 30 60 12 0 223 ± 23.5 4 89 ± 19 .0# 90 7 ± 14 .5# 10 47 ± 33.5# 10 47 ± 12 7* 17 95 ± 13 .7*# 2074 ± 267* 2725 ± 2 41* # 13 17 ± 70.0 30 31 ± 297 *# 416 9 ± 222*# 4 690 ± 17 8*# ... 4 .14 77.4 ± 0 .11 95 .8 ± 0 .12 97 .7 ± 0 .12 0.20 89. 6 ± 4.60 78.6 ± 0 .12 97 .7 ± 0 .10 99 .0 ± 0.02 0 .10 96 .8 ± 4.80 80.8 ± 0.5 10 0 ± 0 .11 10 0 ± 0.03 0.00 10 0 ±0.05 10 0 ± 0. 01 100 ± 0 .11 10 0 ± 0.03 Data ... 15 2× 10 3 4× 10 3 8× 10 3 10 × 10 3 30 60 292 ± 15 .5 415 ± 20.0# 627 ± 26.8# 706 ± 28.5# 395 ± 40 .1* 9 71 ± 7 .1* # 2006 ± 36.6*# 2352 ± 42.0*# 532 ± 39 .1* 13 66 ± 63.5*# 25 61 ± 11 1*# 3 211 ± 66.5*# 12 0...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... Co2+ Cr3+ Cd2+ Concentration (mM) Relative activity (%) – 1. 0 1. 0 0 .1 1.0 1. 0 0 .1 0 .1 1.0 0 .1 1.0 0.5 0.5 10 0 10 0 10 0 10 0 10 0 15 12 4 90 12 0 13 0 35 25 Fig Purification of recombinant human ACMSD I ... compilation ª 2007 FEBS L Pucci et al 10 11 12 13 14 15 16 17 18 19 20 21 quinolinic acid immunoreactivity in Alzheimer’s disease hippocampus Neuropathol Appl Neurobiol 31, 395 –404 Bosco MC, Rapisarda A, ... Neurochem Res 16 , 11 39 11 43 Schwarcz R, Whetsell WO & Mangano RM ( 19 83) Quinolinic acid: an endogenous metabolite that produces axon-sparing lesions in rat brain Science 2 19 , 316 – 318 Burns LH,...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... QGP -1 cells 0.5 B 13 95 2 13 866 13 202 12 068 11 8 71 11 7 81 12 395 Relative luciferase activities in QGPI cells 13 86M luc −2081H luc 19 95 H luc −231M luc 13 31H luc 13 7M luc −524H luc 11 6M ... con- B Exons 1a DN -11 0 DN -10 5 +1 DN -14 4 DN+385 DN+7 21 DN -9 DN+ 89 DN+232 DN+254 DN+785 A 8 81 bp C FEBS Journal 273 (2006) 3 094 – 310 5 ª 2006 The Authors Journal compilation ª 2006 FEBS 895 bp B RT-PCR ... of COS-7 cells ATG −5 TGA +1 0.5 1. 0 coding region ex-pDN +1 exon ex-pDN1 ( +1) ( +15 6) exon 1a ( -11 868) +847 coding region (+243) +1 ex-pDN1a +847 +847 coding region ( -11 770) Fig Demonstration of...
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