1 effects of histone deacetylase inhibitors on cells of ra synovium
Báo cáo khoa học: Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1 gene expression docx
... second, the enzymatic modification of histones, via acetylation, methylation and phosphorylation [15–18] Acetylation by histone acetyltransferases (HATs) occurs on specific lysine residues on the ... promoters [15,16] Conversely, histone deacetylases (HDACs) have also been characterized Hypoacetylation of histones associates with transcriptional silence, and several transcriptional repressors ... the N-terminal tails of histone H2A, H2B, H3 and H4 This neutralization of positive charge leads to a loosening of the histone: DNA structure, allowing access of the transcriptional machinery; furthermore,...
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Báo cáo y học: ""Shock and kill" effects of class I-selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV-1 quiescence" doc
... decreased intracellular levels of reduced glutathione [26,28] The depletion of reduced glutathione has been linked to activation of viral replication [29], whereas the administration of this cofactor ... under control of the HIV-1 LTR [17] In these Jurkat cell clones, GFP induction by HDACIs was evident only in a fraction of cells at 24 hours of incubation and increased over time [see Additional ... Effective concentrations for increasing viral replication to 500% of the basal levels of untreated controls (EC500) Panel D: Cell viability of ACH-2 cells, as measured by the methyl tetrazolium...
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báo cáo khoa học: "New clinical developments in histone deacetylase inhibitors for epigenetic therapy of cancer" doc
... was 45 mg/ m2/day There was inhibition of HDAC activity and induction of acetylation of H3 histones by MGCD0103 A separate phase trial of oral MGCD0103 was conducted in patients with leukemia ... WD: Rational development of histone deacetylase inhibitors as anticancer agents: a review Mol Pharmacol 2005, 68:917-932 Bolden JE, Peart MJ, Johnstone RW: Anticancer activities of histone deacetylase ... induce acetylation of histones, induction of p21, cell cycle growth arrest, and apoptosis but also has been demonstrated to induce acetylation of HSP90 [62] LBH589 IV formulation was investigated...
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Anti inflammatory pharmacology of histone deacetylase inhibitors in pre clinical models of rheumatoid arthritis
... residues on histone tails leads to chromatin condensation and transcription repression [11-13] The participation of HATs and HDACs in gene expression control as well as applications of HDAC inhibitors ... values of HDAC inhibitors on GCP-2 inhibition in THP-1 cells 151 7.4 IC50 values of HDAC inhibitors on MCP-2 inhibition in THP-1 cells 152 7.5 IC50 values of HDAC inhibitors on MIF inhibition in ... ubiquitinylation, sumoylation, carbonylation and glycosylation [11-13] The histone acetylation status controls gene expression as illustrated on Figure 1.2 [11-13] Acetylation of the -NH2 group on lysine residues...
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Epigenetic potential of histone deacetylase inhibitors in treating fibroproliferative diseases and preventing peri implantational fibrosis
... charge of the histones (Fig 4) 21 Fig Biochemical reaction of histone acetylation (Reused with permission from John Wiley & Sons, Inc.: Kuo MH, Allis CD Roles of histone acetyltransferases and deacetylases ... among the histone modifications Reversible histone acetylation usually occurs on the ε-NH3+ groups of highly conserved lysine residues embedded in the N-terminal tails of core histones Histone ... of core histones by histone variants (Ahmad K & Henikoff S 2002; Redon C et al 2002) Among them, histone modifications, including acetylation, methylation, phosphorylation, ADP-ribosylation, ubiquitination...
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Báo cáo y học: "Histone deacetylase inhibitors modulate metalloproteinase gene expression in chondrocytes and block cartilage resorption" ppt
... Acetylation by histone acetyltransferases occurs on specific lysine residues on the N-terminal tails of histones H3 and H4 This neutralisation of positive charge leads to a loosening of the histone: DNA ... Hypoacetylation of histones associates with transcriptional silence, and several transcriptional repressors and corepressors have been identified that have (or R504 recruit) HDAC activity [17-19] Nonhistone ... expression Conceptually, the action of HDAC inhibitors leading to an increase in histone acetylation should induce expression of susceptible genes, but in fact, many instances of a repression of gene...
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báo cáo khoa học: "Novel histone deacetylase inhibitors in clinical trials as anti-cancer agents" ppt
... activity of these inhibitors is thought to be mediated in part by induction of histone acetylation, resulting in a permissive or more open chromatin configuration and potential reactivation of aberrantly ... of the core histones, thereby strengthening histone tail-DNA interactions and blocking access of the transcriptional machinery to the DNA template In addition, histone modifications are specifically ... response duration was months ATRA exerted no additional effect in patients receiving the combination However, of ten VPA responders who relapsed, four achieved a second response after addition of...
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Báo cáo y học: " Histone deacetylase inhibitors induce apoptosis in human eosinophils and neutrophils" docx
... inflammation List of abbreviations (COPD): Chronic obstructive pulmonary disease; (GMCSF): Granulocyte-macrophage colony-stimulating factor; (HAT): Histone acetyltransferase; (HDAC): Histone deacetylase; ... determinations using cells from different donors Kankaanranta et al Journal of Inflammation 2010, 7:9 http://www.journal-inflammation.com/content/7/1/9 Page of 15 Figure The expression of histone deacetylases ... solutions of budesonide (50 mM) were prepared in ethanol The final concentration of ethanol in the culture was 0.2% Stock solutions of HDAC inhibitors were prepared in DMSO The final concentration...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells ... Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells ... the cells synchronization Dose-response effect of growth factors on cell proliferation A 96-well plate with synchronal NP cells was taken and the medium was removed, different concentrations of...
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báo cáo khoa học: " Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells" pot
... the monoplast colony formation of ovarian carcinoma cells Monoplast colony in 50 -cells wells of each group (A): OVCAR-3 cells (B): OVCAR-3-neo cells (C): OVCAR-3-NC cells (D): OVCAR-3-s3 cells ... effects of MACC1 expression for further analysis As a consequence of MACC1 gene knockdown, the proliferation, migration and invasion of OVCAR-3 cells were obviously inhibited, but the apoptosis rate ... 100) Bar graphs show the average rates of monoplast colony formation.*P < 0.05 versus control groups Figure Apoptosis induced by MACC1 RNAi in ovarian carcinoma cells After MACC1 inhibition, cell...
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Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
... 283 ± 28% vs control: it has been shown that the tested concentration produces near-maximal activation of PMNs [35,36,20] Concentrations of the other stimuli were chosen on the basis of published ... mechanisms of inhibitory actions of gliclazide on neutrophils-endothelial cells adhesion and surface expression of endothelial adhesion molecules mediated by a high glucose concentration J Diabetes ... and their inhibition of O2- production and of myeloperoxidase release, these properties being shown in vitro with steep concentrationresponse curves and at high concentrations The two compounds...
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Báo cáo khoa học: Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) potx
... MRP4-expressing cells Results Characterization of the mRNA expression of selected ABC transporters in transfected HEK293 cells To determine the relative mRNA expression of the various ABC transporters of ... following conditions: reverse transcription (20 at 61 °C), one cycle of denaturation at 95 °C for 30 s, and PCR of 45 cycles with denaturation (15 s at 95 °C), annealing (30 s at 58 °C) and elongation ... accumulation in Pgp-expressing cells in a manner that depends on both the concentration of the flavonoids and the level of Pgp expression It has been proposed that the flavonoids interacted directly...
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Báo cáo Y học: Effects of ATP depletion and phosphate analogues on P-glycoprotein conformation in live cells docx
... interact with Pgp in such a manner that preincubation of the cells with UIC2 completely suppresses the subsequent binding of MM12.10 In contrast, UIC2 mAb added at saturating concentration decreased ... indicative of conformational changes propagating to the extracellular domains of the pump upon its interaction with these nucleotides [21] We have recently shown [22] that drug transport-related conformational ... Vi trapping of Pgp also occurs in live cells Coupling of Pgp-mediated drug transport and ATP hydrolysis are generally interpreted in terms of ligandinduced ATPase activation and concomitant transitions...
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Báo cáo khoa học: The effects of a-secretase ADAM10 on the proteolysis of neuregulin-1 pot
... nontransgenic littermates Furthermore, Akt-phosphorylation, which also Fig Processing products of NRG-1 in ADAM10 transgenic mice Soluble and membrane tethered fractions of NRG-1 from brains of ... concentration of lm GM6001 (Calbiochem, San Diego, CA, USA) was used at a final concentration of 10 lm and phorbol 12-myristate 13-acetate (Sigma, Deisenhofen, Germany) was used at a concentration ... differentiation factor is a neuron-glia signal and regulates survival, proliferation, and maturation of rat Schwann cell precursors Neuron 15, 585–596 15 Lai C (2005) Peripheral glia: Schwann cells...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
... effects of 20E both on cell proliferation and morphological changes of IAL-PID2 cells Isolation and characterization of P interpunctella EcR-B1 mRNA Cloning of a PIEcR cDNA frament We wondered ... minimal concentration of 20E required for an induction of PIEcR-B1 mRNA, IAL-PID2 cells were exposed to various concentrations of 20E for 18 h As shown in Fig 5B, a significant induction of PIEcR-B1 ... alone induced a striking decrease of cell proliferation By contrast, in combination with JH II at 10)6 or 10)7 M, cells grew at almost the normal rate Intermediate levels of cell proliferation...
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Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin ppt
... reaction, is dependent on the starting concentration of free thrombin molecules (which itself is dependent on the total TM concentration) (Fig 5B) Consequently, the maximal concentration of this ... numerical integration of the rate equations that describe the mechanism in Fig was performed to obtain theoretical insight into the effect of the second-order rate constants and concentrations of TM and ... concentrations of TM and PAI-1 on the rate of thrombin/ PAI-1 complex formation For various combinations of kon and koff for the thrombin/TM interaction, the concentration of all reactants and intermediates...
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Báo cáo khoa học: Effects of the antioxidant PycnogenolÒ on cellular redox systems in U1285 human lung carcinoma cells docx
... biological effects of selenium are however strictly concentration-dependent, with antioxidant properties at low concentrations and powerful pro-oxidant effects at moderate to high concentrations TrxR, ... here that regulation of TrxR appears to be primarily dependent on the redox state of the cell, rather than the selenium content This is demonstrated by the effect of addition of PycnogenolÒ after ... functions, including reduction of thioredoxin and other low molecular weight substances, and reduction and detoxification of hydroperoxides [9] This reduction may be direct or by regeneration of...
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báo cáo hóa học:" Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cells" potx
... part of the programme endorsed by the Consortium for the Globalization of Chinese Medicine The authors are most grateful to Prof YC Cheng of Yale University and Prof Paul Tam of University of Hong ... ethanol-water extracts of ginseng http://www.translational-medicine.com/content/7/1/34 Figure Inhibition of MAP kinases activation after PGSE treatment Inhibition of MAP kinases activation after PGSE ... a known inhibitor of ERK1/2, on the suppression of TNF-αinduced CXCL-10 transcription (not shown) In contrast, the PGSE did not show any effect on TNF-α-induced activation of p38MAPK and NF-κB...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx
... volume of intact tissue in the ipsilateral hemisphere from that in the contralateral hemisphere Liu et al Journal of Translational Medicine 2011, 9:105 http://www.translational-medicine.com/content/9/1/105 ... differentiation of MSCs: Upon induction with adipocyte induction media cells showed adipocyte globules on oil red ‘O’ staining (D) Osteogenic differentiation of MSCs: Upon induction with osteogenic ... percentage of positivity was mentioned in the brackets Liu et al Journal of Translational Medicine 2011, 9:105 http://www.translational-medicine.com/content/9/1/105 Page of 10 Figure Efficiency of gene...
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báo cáo hóa học: " Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve " doc
... neural cells in the presence of 1.5% of DMSO The rationale for the utilization of the N1E-115 cells was to take advantages of the properties of these cells as a neural-like cellular source of ... mats of fibronectin as a conduit material for use in peripheral nerve repair J Hand Surg (Br) 1995, 20:429-36 22 Ahmed Z, Brown RA: Adhesion, alignment, and migration of cultured Schwann cells on ... application These hfPS cells, in contrast to Embrionic stem cells, N1E-115 cells after in vitro differentiation and induced pluripotent stem cells, not require any genetic manipulation, are readily...
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