... CCAAT box at 324 bp upstream of the major transcription start site. The presence of a CCAATbox and the absence of a TATA box seem to be one of the characteristics ofthe mouse integrin a3 gene.We ... upstream ofthe SacI) sites (Fig. 1).Sequence analysis ofthe 5Â-anking region anddetermination of transcription start sites of mouseintegrin a3 subunit gene The nucleotide sequence ofthe 0.5 ... C/EBP (CCAAT) is boxed. The translationstart site (ATG) and the cleavage site in the processing ofthe poly-peptide (arrow) are also shown. The nucleotide sequence of the 5Â-anking region and...
... imagine the old knight, onlyhalf aware ofthe sunshine ofthe evening, the noise ofthe streets, the looks ofthe crowd, the great minsterrising half-finished in the midst ofthe town by the Rhine, ... Boiardo, ofthe Ferrarese court to which he belonged, and ofthe school of poetry (including the more notable but less original work of Ariosto) which the genius ofthe man and the character ofthe ... select their wives, instead of beingselected; and once the woman and the dowry are in their hands, trouble themselves but little whether they are approved of or not. On the other hand, the mistress...
... compared to the wildtype Arg46, with the precise value depending on the identity ofthe substituentamino acid (Fig. 5). These data establish that the mutantreceptors are much less likely than the ... increasingconcentrations of AVP, was assayed for each ofthe 19mutant constructs and the dose–response characteristicscompared to the wildtype V1aR. None ofthe 19 encodedamino acids could substitute the Arg46 ... mutation of Arg46 had the dualeffect of (a) decreasing agonist affinity and (b) promoting the agonist-induced active conformation due to the loss of astabilizing constraint on the ground state of the...
... remain of a technical nature (i.e. ex-post behaviour ofthe model to policy changes in the baseline) and are therefore also not further commented in the report. The results of scenario S4 are ... the baseline. The amounts ofthe payments are considered a simulation outcome, because they depend on production and they are thus presented in the section of scenario results. The core assumptions ... coupling of 10% ofthe total payment ceilings for sub-sectors. In CAPRI, the decoupled payments are modelled as payments per hectare of land, with the same amount per hectare applying regardless of...
... as 100 · (arbitrary unit of each band in individual lane divided by the unit ofthe unbound RNA detected in the absence ofthe N protein). The results are representative of four independent experiments. ... copies ofthe N protein, respectively. (C) The RNA binding efficiency of N protein is represented as a percentage of bound RNA (i.e. the percentage of bound RNA = 100%)percentage of free probe). The ... phosphorylation.Effect of RS motif phosphorylation onoligomerization ofthe N proteinTo better understand the effect of RS motif phosphor-ylation on the biological function ofthe N protein, weFig. 3. The translation...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes. The orientation ofthe ... at position E7 as the source ofthe H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... ligand.Comparison ofthe strengths of dipolar interactions insolution with the alternate crystal structures of methemo-globin establish that the solution structure of wild-type Hbmore closely resembles the...
... in 7b compared with 7a (Table 1) because ofthe b-effect ofthe acetylation. Acetylation of 7b was confirmed byESI MS data, which gave the expected mass of 3221.4 Da. The spectra of oligosaccharide ... and showed, in particular, the configuration of ManNAc to beD.From the combined the data on the structures of the isolated oligosaccharides, the overall structure of the P. frisingensis LPS carbohydrate ... Oligosaccharides 1a and 1b are the fragments ofthe larger structure 2, thus the NMRdata for 1a,b are very close to those for the respectiveresidues in 2 and are not presented. The position and anomeric...
... kinetic properties of NR1, and as we have shown for CPR and P450 BM3(23,24,28) the properties ofthe flavin binding domains of NR1 are likely to mimic the redox properties ofthe domainsin full-length ... enzymes, the potentials for the FAD/NADPHdomains are rather more negative than those for the FMNdomain, reflecting the direction of electron transfer fromNADPH through FAD, then FMN and on to the ... The presence ofthe His-tag on the FAD/NADPH domain had no apparent effecton catalytic activity. The purified domains were yellow, indicating the presence of bound cofactor, and they displayed...
... LPS)[5,6]. The serological specificity ofthe bacteria is definedby the structure ofthe O-specific polysaccharide chain(O-antigen) ofthe lipopolysaccharide. On the basis of the O-antigens, the strains ... the Dconfiguration (the b effect on C3 of L-GlcAwouldbeclosetozero[27]).On the basis ofthe data obtained, it was concluded that the repeating unit ofthe O-polysaccharide of P. myxofac-iens has the following ... The Dconfiguration of GlcN andGalN was established by GLC ofthe acetylated (S)-2-butylglycosides. The Dconfiguration of GlcA was determined byanalysis of the 13C NMR chemical-shift data of the polysaccharide...
... amounts ofthe ECDs ofthe non-a sub-units of human muscle AChR for use as starting material for the determin-ation ofthe 3D structure ofthe receptor ECDs and for the characterization of the ... domain [3]. The N-terminal ECD of each ofthe two a subunits con-tains the major part ofthe binding site for the cho-linergic ligands. The two sites are nonequivalent, onebeing formed at the interface ... datafrom electron images ofthe AChR found in largeamounts in the electric organ ofthe marine ray,Torpedo californica [3]. The acquisition ofthe crystallo-graphic structure ofthe mollusc acetylcholine-bindingprotein...
... demonstrated the role of PufX in the orientation ofthe RC inside the LH1Fig. 4. Isolation ofthe PMCs on a sucrose gradient. The final detergentextracts from the ICM were loaded on the top of a 10–40% ... PufXD2)7neither affects the photo-synthetic capability ofthe bacteria nor the efficiency of exchange ofthe ubiq uinol molecules between th e RC and the bc1, a s judged from the reduction kinetics of ... residues ofthe LH1 apolypeptide in the organization ofthe LH1–RC complex[12]. In the present work, we have investigated the possible involvement ofthe N-terminus and of the C-terminus of PufX...
... power, in the beauties of holiness; from the womb ofthe morning thou hast the dew of thyyouth. There were many evident proofs ofthe truth of this since the beginning ofthe plantation ofthe gospelinto ... and feet, Europe, and the toes the Isles ofthe Sea the British Isleswith the rest. And the form ofthe great earth-filling figure is that of Jesus of Nazareth, the Man of Jehovah'sright ... not at all the question? For if reformation of religion be the case of ouraffairs; then either the parliament are they that do it, or the cavaliers: not the cavaliers, for they are on the defensive:...
... outlining the historical context of health and media studies, the authors describe major individual-level theoretical frameworks applicable in the area: social cognitive theory, the theory of reasoned ... communicate with parents (see Melton 2009). Authors estimate 5,000 blogs in the U.S. devoted to education, but there is no substantiation of this figure, and most ofthe blogs discuss areprofessional ... but whether and how these alternative information sources are effectively supplementing the apparent declines in traditional information sources (in terms of both whatthe produce and the extent...
... surplus in Japan. The pur-pose of this study is to project the future growth of the Japanese medical workforce and to forecast whether the proposed additional increase in the student quota willcause ... age, specialty, address, andplace of work. These reports are tallied and published astheSurveyofPhysicians[13].Thenumberofmedicaldoctors in our baseline year of 2008, stratified bysex and age, ... may reduce the quality of doctors and that of medical treatment.Furthermore, an increase in the medical student quotamay reduce the number of science and e ngineeringstudents or their a verage...