... mechanism of egg envelope digestion is discussed on the basis of the manner of the reciprocal or cross-species egg envelope digestion using enzymes and substrates of both species: zebrafish and ... other fish species, such as the Japanese eel belonging to Elopomorpha that is sister to the common ancestor of zebrafish and medaka The present study isthe first approach aiming to fully understand ... markers, and those on the right are the molecular masses for major bands sites (Fig 6, sites A, E and B) The results suggest that ZHE1 and MHCE have the same substrate specificity toward zebrafish unfertilized...
... groups of two polymers would be 90–360 ⁄ n degrees If the distance between the )SH sites is approximately 90°, and the side of the Hp subunit contributes the base of the triangle, the distance is ... ruminants and pig, as pig possesses only the Hp 1-1 phenotype [24] As dolphins and whales are the closest divergences before the ruminants, we further examined the size of the a-chain in whales and ... possess Hp as the sole genotype [25] It is likely that ruminants including deer, cattle, goat and sheep may all possess a sole Hp 2-type allele In the present study, we have shown that the inserted...
... distant relatives, including non-plant species It is possible to move genes between such seemingly unrelated organisms because all living organisms share the same code for DNA and the synthesis ... organisms, e.g plants, or cells so that changes in the organism 's DNA will occur Such changes are then evaluated for their beneficial effects, such as disease resistance Natural selection: The ... uses similar random processes to cause changes in a plant s genes Plants then are assessed to determine if the genes were changed and whether the changes provided a beneficial trait such as disease...
... Dis SI Total Figure Mean scores and standard errors of PDQ-39 domains by Hoehn and Yahr stages of illness Mean scores and standard errors of PDQ-39 domains by Hoehn and Yahr stages of illness ... severity assessment was performed during the recruitment visit, using the Unified Parkinson 's Disease Rating Scale (UPDRS version 3.1) and Hoehn and Yahr (HY) staging system [4,5] All assessments of ... and Yahr stages, UPDRS scores and main symptoms for assessment of construct validity All these studies found statistically significant differences between Table 4: Mean, standard deviation (SD)...
... still attracts the largest audiences of any play in Germany whenever it s performed Faust isthe story of a scholar who makes a deal with the devil The devil promises him anything he wants as ... human thought, including - especially including the humanities and arts Science unleavened by the human heart and the human spirit is sterile, cold, and self-absorbed It s also unimaginative: some ... interest you I started out as a classics major I’m now Professor of Biochemistry and Chemistry Of all the courses I took in college and graduate school, the ones that have benefited me the most...
... Discussion Understanding the mechanism through which the miR-17-92 locus is able to promote cellular proliferation and tumorigenesis in multiple cell lines and tissues is essential if miRNAs ... and metabolism in model organisms, some miRNAs have also been classified as tumor suppressors or oncogenes (reviewed in [1]) The first reported and most well studied oncomiR isthe human miR-17-92 ... dissect the mechanisms of the miR-17-5p proliferative response, it was necessary to determine the endogenous mRNA targets of this miRNA Predicted target sites were cloned into the 3' untranslated...
... industrious, agreeable, and popular old soul, she is a walking sermon on the vanity of feminine prettiness Just as Redpenny has no discovered Christian name, she has no discovered surname, and is known ... figure and manner he is more the young man than the titled physician Even the lines in his face are those of overwork and restless scepticism, perhaps partly of curiosity and appetite, rather than ... [She goes out] Sir Colenso Ridgeon is a man of fifty who has never shaken off his youth He has the offhanded manner and the little audacities of address which a shy and sensitive man acquires...
... colonists themselves This exasperated "the Sons of Liberty", and they met and resolved to resist this new act of oppression to the last The troops arrived in due time, and of course collisions took ... financial crisis, that was so wide-spread and disastrous that the lower classes suffered for want of food Banks suspended specie payment, manufactories were forced to stop work, and paralysis ... position beside our successful commanders and brave soldiers It is also important, as a lesson for the future, and naturally brings up the question, what are the best measures, and what is the...
... licensed psychologist specializing in sex offenders, risk assessment and the law, trained the research team in how to assess the competence of research participants to consent/assent to the research, ... subject In this study, screening potential research subjects was envisioned as a two-step process: assessing their eligibility over the phone (i.e., asking questions to assess whether they were ... find and interview this population, and how to best handle the issues that might arise Based upon the expertise of Ms Lloyd and the staff at GEMS, as well as other experts who work with these girls...
... Tom Hanks đến tham quan Xem hoà nhạc giáng sinh nhà thờ St John the Divine Nhà thờ St John the Divine công trình kiến trúc đặc s c thành phố NewYork nhà thờ lớn Mỹ Vào ngày trước sau Giáng sinh ... chân thông s n trượt băng nghệ thuật khiến cho khung cảnh giáng sinh thêm nhiều màu s c Chơi đàn chân cửa hàng đồ chơi FAO Schwartz Không khí Giáng sinh nhộn nhịp giới đồ chơi FAO Schwartz không ... nhà thờ Các chương trình ca nhạc biểu diễn nghệ s chuyên nghiệp, mục s ca s nghiệp dư khách tham quan Ngất ngây vườn thực vật Botanical Garden Botanical Garden xây dựng từ năm 1981, bảo tàng...
... DRIVERS OF FAILURE What, then, does account for the success and failure of entrant and established firms? The following discussion synthesizes from the history of the disk drive industry a new perspective ... the success and failure of the industry s best firms Simply put, when the best firms succeeded, they did so because they listened responsively to their customers and invested aggressively in the ... markets they serve LESSONS FOR SPOTTING DISRUPTIVE THREATS AND OPPORTUNITIES Some managers and researchers familiar with these ideas have arrived at this point in the story in an anxious state...
... transport As ATP-driven Na+ transport would also require the stator subunits, one has to assume that previous preparations had substoichiometric amounts of subunits a and b This is in accordance ... used here was competent in ATP synthesis This underlines the essential function of the stator subunits in driving ion gradient-driven ATP synthesis As observed before with the Na+ F1F0-ATP synthase ... membrane motor Most important, both types of c subunits were present This made it possible to readdress the longstanding question of whether an 3422 ATPase with F0- and V0-type rotor subunits is...
... RNF13 has a TM sequence Consistent with this, our analysis of cellular membranes washed with high-salt solution establishes that RNF13 is indeed an integral membrane protein This is consistent ... predicts that this domain contains two N-linked glycosylation sites, residues 43 and 88 Consistent with synthesis on the ER, the program tmpred [20] predicts that residues 182– 203 comprise a 22-residue ... bands at 80 kDa and the group of bands at 65 kDa were recognized by the anti-HA serum (Fig 4B, lanes and 5) As these proteins are also recognized by the anti-FLAG serum, they must possess both...
... analysis, subcloning, DNA digestions, and lambda phage and plasmid DNA isolations [23] Chromosomal DNA was isolated as previously described [24] Sequence analysis was performed on both strands of ... using PNP-a-L-arabinofuranoside as a substrate This most likely means that the removal of the L-arabinose residues from the galactomannan oligosaccharides is caused by traces of an enzyme in the ... incubations (20 h) D-galactotriose also disappears To determine whether a small amount of a-L-1,3-arabinofuranosidase was responsible for the disappearance of Fig HPAEC analysis of the hydrolysis of...
... with the matrix solution (a-cyano-4-hydroxycinnamic acid) for MALDI-TOF MS analysis Structure comparison A search of the SwissProt-TrEMBLE database, pairwise and multiple sequence alignments, and ... maintain the quaternary structure of the protein Below pH 2.5, where the protein dissociates, the depolymerization process is accompanied by the almost complete loss of secondary structure This is indicated ... in L chains This characteristic, first described for the polypeptide chains of bullfrog ferritin [9], is common to fish ferritins on the basis of the available sequences A further distinctive property...
... by desA and its possible involvement in desferrioxamine biosynthesis, two apramycin-resistant and kanamycin-sensitive transformants were isolated among S coelicolor transformants with the desA ... and the ribosome-binding site is underlined The ATG is shown in bold letters The reported transcription start site of theS pilosus desA promoter is indicated by three asterisks Note the strict ... indicates that the desABCD cluster is expressed as a polycistronic 1116 transcript Expression of the four genes is coordinately regulated by iron limitation, as shown by the RT-PCR analysis, and there...
... sequence to distinguish the products resulting from the expression of the DNA constructs and the endogenous gene (Fig 2) These constructs were transfected in Hec-1B cells and the cells were subsequently ... newly synthesized polyadenylated IFN-b mRNA (Fig 5) This observation suggests that the deadenylation machinery is pre-existing in the cells and deadenylates IFN-b mRNA as soon as its synthesis ... not shown) Discussion In the present study, we analysed the expression and the poly(A) status of human IFN-b mRNA in human endothelial Hec-1B cells in response to infection by the Sendaı¨ virus...
... overexpress, distinct bands corresponding to LIN54 and the DHCH and DN mutants could be detected (marked by asterisks) Fluorescence-activated 5706 cell sorting studies did not suggest that the expression ... antiserum has been described previously [12] The LIN54 antiserum was raised in rabbits against the peptide AAKTKLSSQISDLLTR The LIN54 serum was affinity purified Other antibodies used were a-E2F4 ... these data establish LIN54 as an essential member of the LINC ⁄ DREAM complex delayed entry into mitosis [9] To address whether this isan isolated function of LIN9 or whether it is mediated by...
... (dotted and dashed lines), and Br-DSPC/cholesterol liposomes (long dashed lines) After 10 at room temperature, the fluorescence emission spectra were measured Maximal emission wavelengths in the presence ... reading the manuscript This work was supported by grants from the Japan Science and Technology Corporation, the Japan Science Society (to Y .S. ) and from a Grant-in-Aid for Scientific Research from the ... in membranes These results show that cholesterol-binding activity resides in domain 4, and the binding specificity isthe same as that of h-toxin They also indicate that the amino acid sequence...