biểu hiện podoplanin trong ung thư tế bào gai hốc miệng và liên quan di căn hạch

BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ Y TẾ ĐẠI HỌC Y DƯỢC TP HỒ CHÍ MINH - PHẠM ĐỖ THANH THANH BIỂU HIỆN PODOPLANIN TRONG UNG THƯ TẾ BÀO GAI HỐC MIỆNG VÀ LIÊN QUAN DI CĂN HẠCH LUẬN VĂN THẠC SĨ RĂNG - HÀM - MẶT THÀNH PHỐ HỒ CHÍ MINH - NĂM 2020 BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ Y TẾ ĐẠI HỌC Y DƯỢC TP HỒ CHÍ MINH - PHẠM ĐỖ THANH THANH BIỂU HIỆN PODOPLANIN TRONG UNG THƯ TẾ BÀO GAI HỐC MIỆNG VÀ LIÊN QUAN DI CĂN HẠCH NGÀNH: RĂNG – HÀM – MẶT MÃ SỐ: 8720501 LUẬN VĂN THẠC SĨ RĂNG - HÀM - MẶT NGƯỜI HƯỚNG DẪN KHOA HỌC: TS NGUYỄN THỊ KIM CHI PGS.TS NGUYỄN THỊ HỒNG THÀNH PHỐ HỒ CHÍ MINH - NĂM 2020 LỜI CAM ĐOAN Tôi xin cam đoan cơng trình nghiên cứu riêng tơi Các liệu kết nêu luận văn trung thực chưa công bố cơng trình khác Ký tên Phạm Đỗ Thanh Thanh MỤC LỤC DANH MỤC TỪ VIẾT TẮT i ĐỐI CHIẾU THUẬT NGỮ ANH – VIỆT iv DANH MỤC BẢNG vi DANH MỤC BIỂU ĐỒ viii DANH MỤC HÌNH ix MỞ ĐẦU CHƯƠNG TỔNG QUAN TÀI LIỆU 1.1 Bệnh học ung thư hốc miệng 1.2 Podoplanin ung thư hốc miệng CHƯƠNG ĐỐI TƯỢNG VÀ PHƯƠNG PHÁP NGHIÊN CỨU 13 17 2.1 Đối tượng nghiên cứu 17 2.2 Phương pháp nghiên cứu 18 2.3 Khía cạnh đạo đức nghiên cứu 30 CHƯƠNG KẾT QUẢ NGHIÊN CỨU 31 3.1 Đặc điểm lâm sàng 31 3.2 Đặc điểm giải phẫu bệnh 33 3.3 Biểu Podoplanin 36 3.4 Mối liên quan kiểu biểu Podoplanin với đặc điểm lâm sàng giải phẫu bệnh 40 3.5 Mối liên quan kiểu biểu Podoplanin với di hạch giải phẫu bệnh 44 3.6 Mối liên quan mức độ biểu Podoplanin với đặc điểm lâm sàng giải phẫu bệnh 45 3.7 Mối liên quan mức độ biểu Podoplanin với di hạch giải phẫu bệnh 50 CHƯƠNG BÀN LUẬN 51 4.1 Đặc điểm lâm sàng 51 4.2 Đặc điểm giải phẫu bệnh 55 4.3 Biểu Podoplanin 58 4.4 Mối liên quan kiểu biểu Podoplanin với đặc điểm lâm sàng giải phẫu bệnh 62 4.5 Mối liên quan kiểu biểu Podoplanin với di hạch giải phẫu bệnh 64 4.6 Mối liên quan mức độ biểu Podoplanin với đặc điểm lâm sàng giải phẫu bệnh 64 4.7 Mối liên quan mức độ biểu Podoplanin di hạch giải phẫu bệnh 70 4.8 Hạn chế nghiên cứu 71 4.9 Ý nghĩa tính ứng dụng đề tài 71 KẾT LUẬN 72 KIẾN NGHỊ VÀ ĐỀ XUẤT HƯỚNG NGHIÊN CỨU 73 TÀI LIỆU THAM KHẢO PHỤ LỤC DANH MỤC TỪ VIẾT TẮT VIẾT TẮT VIẾT NGUYÊN CHỮ AJCC American Joint Committee on Cancer Ang1 Angiopoietin-1 α-SMA Alpha-Smooth Muscle Actin BVUB Bệnh Viện Ung Bướu CAF Cancer – Associated fibroblast CAR-T Chimeric Antigen Receptor T cell CCL21 Chemokine (C-C motif) Ligand 21 CD9 Cluster of Differentiation CD44 Cluster of Differentiation 44 CDK5 Cyclin-Dependent Kinase CLEC-2 C-type LECtin like receptor c.s Cộng CT Computed Tomography DAB 3’- Diaminobenzidine DNA Deoxyribonucleic Acid Ebp1 ErbB3 binding protein E-cadherin Epithelial cadherin EDTA Ethylenediaminetetraacetic Acid EGFR Epidermal Growth Factor Receptor EMT Epithelial to Mesenchymal Transition ERK Extracellular signal-regulated kinase ERM Ezrin– Radixin– Moesin FGF-2 Fibroblast Growth Factor GLOBOCAN Global Burden Of Cancer GPB Giải Phẫu Bệnh GTP Guanosine Triphosphate Gy Gray HE Hematoxylin and Eosin HMMD Hóa mơ miễn dịch HSPA9 Heat Shock Protein family A member IFN-γ Interferon gamma IL-6 Interleukin-6 ITG5A Integrin Alpha kDa Kilo Dalton LSAB Labeled StreptAvidin Biotin MMP Matrix Metalloproteinase MMP-9 Matrix Metallopeptidase MMP-14 Matrix Metallopeptidase 14 MRI Magnetic Resonance Imaging mRNA Messenger Ribonucleic acid NF Normal Fibroblast PDGF-BB Platelet – Derived Growth Factor - BB PDPN Podoplanin PET Positron Emission Tomography PET-CT Positron Emission Tomography – Computed Tomography PKA Protein Kinase A PLAG Platelet Aggregation pN Pathological lymph Node metastasis PTCH1 Patched-1 RhoA Ras Homolog gene family, member A RNA Ribonucleic Acid ROCK Rho – associated protein Kinase SEER Surveillance, Epidemiology, and End Results SHH Sonic Hedgehog SMA Smooth Muscle Actin SMO Smoothened SPSS Statistical Package for Social Sciences TBST Tris Buffered Saline with Tween 20 TGF-β1 Transforming Growth Factor beta TNM Tumor Node Metastasis Tp.HCM Thành phố Hồ Chí Minh UTHM Ung thư hốc miệng UICC Union for International Cancer Control WHO World Health Organization ĐỐI 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