Tìm hiểu các enzym bảo vệ ôxy hóa của nấm linh chi ganoderma lucidum

TIM HIEU CAC ENZYM BAO VE OXI HOA CUA N A M LINH CHI GANODERMA LUCIDUM TA BICH THUAN, PHAM KIEN CUdNG, DANG QUANG HUNG Khoa Sinh hoc, Dai hoc KHTN Mddau Nain Linh Chi (Ganoderma lucidum) tir lau da duge coi nhu la mot loai "than dugc" vdi nhiing tac dung gia tri dugc Ueu nhu bd gan, than, phdi, dieu boa huye't ap, lam giam lugng dudng va colesteron mau Gan day, nain Linh Chi dugc diing de dieu tri cac benh ung thu va dugc xem la nguyen lieu chira nhieu chat chdng oxy boa, lao boa cao Nbam ddng gdp them cac dan lieu ve loai nam ed nhieu gia tri dugc lieu nay, chiing tdi da tien hanh tim hieu cac enzyme bao ve oxi boa cua nam Linh Chi, dd trude he't tap trung vao enzym chinh dd la eatalase, superoxid dismutase va NADH oxidase Sau day gidi thieu mot so ket qua ma chiing tdi da thu dugc Nguyen lieu va phi/ctng phap Nguyen lieu Nam Linh Chi Ganoderma lucidum - ky hieu GaL dugc la'y tit phdng Gidng na'm gd'c ciia Trung tam Cdng nghe sinh hoc, Dai bgc Qudc Gia Ha Ndi Phuang phap nghien cicu - Nudi ca'y nam Ganoderma lucidum dugc trinh bay [1,2] - Protein dugc xac dinh theo phuang phap cua Lowry, diing casein lam cha't chu£n [7] - Hoat SOD dugc xac dinh theo phuang phap McCord va Fredovicli [3,8] 784 - noai ug caiaiase ouge xac oinh theo phuang phap cua Thibodeau va cdng su [9] va lugng FIjOz lai duge xac dinh theo phuang phap cua Mottola va cdng su [10] - Hoat cua NOX dugc xac dinh theo phuang phap cua Poole va Clairborne [11] - Dien di tren gel polyacrylamide dugc tien hanh theo phuang phap cua Laemmli [6] - Tinh sach enzyme bang cot sac ky trao ddi ion qua cdt DE-52, sac ky Igc gel qua cdt Sephadex GlOO [4,5] Ket qua thao luan Sir sinh trudng va phat trien ciia nam GaL Ket qua nghien ciiu ciia chung tdi cho tha'y he sgi ciia nam GaL phat trien thich bgp d 26-28°C Sau 18-24 gid ca'y, he sgi bat dau phat trien manh tren mdi trudng nudi ca'y long Chung Ganoderma lucidum cd td'c sgi mge tir 150 - 200 ml/gid Cdn dieu kien nudi cay sinh qua the, ke't qua cho tha'y sau khoang 25-30 nudi ca'y, he sgi na'm bat dau ben ket Sau khoang 2-3 thang ke tir ca'y gidng, tan na'm phat trien thue, mdi bich ehi cho 1-2 tan na'm Idn, nang sua't thu hai tuang ddi thap tir 2,5 - 4% Hoat cac enzym NADH, CAT, SOD ciia nam GaL Hoat dd enzvm NADH oxidase (NOX) cua nam GaT, NOX sinh khoi NOX qua the Hinh L Hoat NADH oxidase (NOX) cua nam GaL 785 Ke't qua xac dinh boat ciia enzym NADH oxidase dugc trinh bay d hinh Qua day chiing tdi thay rang boat NOX cua na'm GaL nhin chung la tuang ddi thap, chi dat 0,049 dan vi/ g nguyen lieu tuoi Hoat NOX cua na'm GaL a dang sinh kbdi cao ban so vdi boat NOX d dang qua the dat 0,029 dan vi/g nguyen lieu tuai Hoat enzym eatalase (CAT) cua nam GaL Catalase (CAT) la enzym chii yeu giii vai trd quan trgng phan buy cac H2O2 hSu bet cac ca the sinh vat Ke't qua xac dinh boat CAT cua mSu GaL (hinh 4) cho thay boat CAT ciia nam GaL nhin chung la tuang ddi cao, dat tuang ling la 2,331 dv/mg protein va 2,319 dv/mg protein d dang qua the va dang sinh kbdi 2.34 s 2.33 '