NGHIÊN CỨU CỐ ĐỊNH ENZYME CHOLESTEROL OXYDASE TRÊN CẢM BIẾN NANO SINH HỌC DỰA TRÊN CẤU TRÚC SỢI NANO PLATIN ĐỂ ĐỊNH LƢỢNG CHOLESTEROL TRONG DUNG DỊCH

BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƢỜNG ĐẠI HỌC NÔNG LÂM THÀNH PHỐ HỒ CHÍ MINH BỘ MƠN CƠNG NGHỆ SINH HỌC ************** KHÓA LUẬN TỐT NGHIỆP NGHIÊN CỨU CỐ ĐỊNH ENZYME CHOLESTEROL OXYDASE TRÊN CẢM BIẾN NANO SINH HỌC DỰA TRÊN CẤU TRÚC SỢI NANO PLATIN ĐỂ ĐỊNH LƢỢNG CHOLESTEROL TRONG DUNG DỊCH Ngành học : CÔNG NGHỆ SINH HỌC Sinh viên thực : NGUYỄN HỮU TUYỂN Niên khóa : 2008 - 2012 Tháng 7/2012 BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƢỜNG ĐẠI HỌC NÔNG LÂM THÀNH PHỐ HỒ CHÍ MINH BỘ MƠN CƠNG NGHỆ SINH HỌC ************** KHÓA LUẬN TỐT NGHIỆP NGHIÊN CỨU CỐ ĐỊNH ENZYME CHOLESTEROL OXYDASE TRÊN CẢM BIẾN NANO SINH HỌC DỰA TRÊN CẤU TRÚC SỢI NANO PLATIN ĐỂ ĐỊNH LƢỢNG CHOLESTEROL TRONG DUNG DỊCH Hƣớng dẫn khoa học Sinh viên thực TS TỐNG DUY HIỂN NGUYỄN HỮU TUYỂN ThS ĐẶNG NGỌC THÙY DƢƠNG Tháng 7/2012 LỜI CẢM ƠN Xin chân thành gửi lời cảm ơn sâu sắc đến:  Ban giám hiệu Trƣờng Đại học Nơng Lâm TP Hồ Chí Minh tạo điều kiện cho học tập trƣờng  Ban chủ nhiệm Bộ môn Công nghệ Sinh học tất quý thầy cô Trƣờng Đại học Nông Lâm TP Hồ Chí Minh truyền đạt kiến thức cho tơi suốt q trình học tập trƣờng  Ban giám đốc Phòng thí nghiệm Cơng nghệ Nano – Đại Học Quốc Gia TP Hồ Chí Minh tạo điều kiện cho tơi thực khóa luận tốt nghiệp  Quỹ Phát triển Khoa học Công nghệ Quốc gia – Nafosted hỗ trợ kinh phí cho tơi thực đề tài Mã số đề tài 106.99-2010.52  TS Tống Duy Hiển, ThS Đặng Ngọc Thùy Dƣơng, chị Phan Thị Hồng Thủy anh Phạm Xuân Thanh Tùng tận tình hƣớng dẫn cho tơi suốt q trình thực hồn thành khóa luận tốt nghiệp  Tất Anh, Chị Phòng thí nghiệm Cơng nghệ Nano giúp đỡ tơi q trình thực khóa luận tốt nghiệp Đặc biệt, xin gửi lòng biết ơn sâu sắc đến ba mẹ sinh thành, nuôi dƣỡng, dạy dỗ, động viên tinh thần cho luôn bên Em xin cảm ơn anh Hai, chị Ba, anh Tuyên ngƣời thân gia đình ln ln động viên giúp đỡ em Cảm ơn tất bạn lớp DH08SH động viên giúp đỡ suốt q trình học tập thực khóa luận tốt nghiệp TP Hồ Chí Minh, tháng năm 2012 Nguyễn Hữu Tuyển i TÓM TẮT Đề tài “Nghiên cứu cố định enzyme cholesterol oxydase cảm biến nano sinh học dựa cấu trúc sợi nano Platin để định lƣợng cholesterol dung dịch” đƣợc thực từ tháng 01/2012 đến tháng 07/2012 Phòng thí nghiệm Cơng nghệ Nano – Đại học Quốc gia Thành phố Hồ Chí Minh với vật liệu nghiên cứu cảm biến sinh học sợi nano Platin enzyme cholesterol oxydase Khóa luận gồm hai nội dung nghiên cứu Nội dung cố định enzyme lên sợi nano Platin Trong nội dung này, trình làm bề mặt cảm biến quy trình biến đổi bề mặt cảm biến với trình tự lớp đơn tự ghép cysteamine, chất hữu trung gian glutaraldehyde, enzyme cholesterol oxydase đƣợc khảo sát Nội dung nghiên cứu ứng dụng cảm biến đƣợc cố định enzyme để kiểm tra định lƣợng nồng độ cholesterol từ 0,8 – mM dung dịch PBS phƣơng pháp quét dòng tuần hồn (cyclic voltammetry - CV) Kết trình xây dựng quy trình biến đổi bề mặt chip cố định enzyme cho thấy nồng độ dung dịch cysteamine tối ƣu 0,5 M; thời gian phản ứng 40C nồng độ dung dịch glutaraldehyde tối ƣu 1%; thời gian phản ứng 40C Cố định thành công enzyme cholesterol oxydase lên cảm biến sau đƣợc biến đổi bề mặt enzyme giữ đƣợc hoạt tính sau cố định Kết trình kiểm tra định lƣợng cholesterol nồng độ khác dung dịch PBS cảm biến sau cố định enzyme cho thấy ngƣỡng phát cholesterol 0,8 mM, xác định đƣợc đỉnh oxy hóa – khử +0,625 V Quá trình kiểm tra định lƣợng cholesterol cho thấy có mối tƣơng quan tuyến tính chặt chẽ cƣờng độ dòng thu đƣợc nồng độ cholesterol khác theo phƣơng trình Ipeak = 13,97*Ccholesterol + 221 với hệ số tƣơng quan R2 = 0,974 Với kết đạt đƣợc, đề tài “Nghiên cứu cố định enzyme cholesterol oxydase cảm biến nano sinh học dựa cấu trúc sợi nano Platin để định lƣợng cholesterol dung dịch” bƣớc khởi đầu cho trình chế tạo thiết bị cảm biến định lƣợng cholesterol máu ngƣời ii SUMMARY The thesis “Study on the immobilization of cholesterol oxydase on bionanosensor structure based on Platinum nanowires to quantify cholesterol in solution” was carried out at laboratory for Nanotechnology National University Ho Chi Minh city from January 2012 to Junly 2012 with the main materials are Platinum nanowires and cholesterol oxydase The thesis has two main subjects The first, immobilization of enzyme on Platinum nanowires In this part, the process of cleaning sensor suface and process modified electrodes suface with cysteamine self-assembled monolayer, the intermediates organic glutaraldehyde and enzyme cholesterol oxydase will be investigated In the next part, use sensors applications have been fixed with enzyme cholesterol oxydase to check, quantify the cholesterol has concentration from 0,8 to mM in PBS solution by using Cyclic Voltammetry mode in Autolab machine The resulfs were summarized as follows:  Complete the process modified electrodes suface through cysteamine self- assembled monolayer and organic glutaraldehyde with the best solution concentration cysteamine is 0,5 M, it react in two hours at 40C The best solution concentration glutaraldehyde is one percent, it react in two hours at 40C  The cholesterol oxydase enzyme was immobilized successfully on the modified surface of Platinum nanowires electrode  Checking and building successfully standard equation The regression equation for cholesterol concentrations and its relevant oxydation current reponse was Ipeak = 13,97*Ccholesterol + 221 with an approximate correlation of R2 = 0,974 and sensor sensitivity of 0,8 mM The experiment results also express the specific oxidation peak at +0,625 V The thesis “Study on the immobilization of cholesterol oxydase on bionanosensor structure based on Platinum nanowires to quantify cholesterol in solution” is the beginning in a whole process of studying about cholesterol meter for cholesterol detection in Human blood Keywords: Biosensor, cholesterol oxydase, Self-assembled monolayer (SAM), cysteamine, glutaraldehyde iii MỤC LỤC Trang LỜI CẢM ƠN i TÓM TẮT ii SUMMARY iii MỤC LỤC iv DANH SÁCH CÁC CHỮ VIẾT TẮT vii DANH SÁCH CÁC BẢNG viii DANH SÁCH CÁC HÌNH ix Chƣơng MỞ ĐẦU 1.1 Đặt vấn đề 1.2 Mục tiêu đề tài 1.3 Nội dung thực Chƣơng TỔNG QUAN TÀI LIỆU .3 2.1 Giới thiệu cholesterol ảnh hƣởng cholesterol đến sức khỏe 2.2 Một số phƣơng pháp cố định enzyme sinh học 2.2.1 Phƣơng pháp nhốt enzyme 2.2.1.1 Phƣơng pháp nhốt enzyme gel .5 2.2.1.2 Phƣơng pháp nhốt enzyme hệ sợi 2.2.1.3 Phƣơng pháp tạo màng bọc nhốt enzyme 2.2.2 Phƣơng pháp tạo liên kết 2.2.2.1 Phƣơng pháp tạo liên kết chéo phân tử enzyme 2.2.2.2 Phƣơng pháp tạo liên kết enzyme chất mang .8 2.3 Giới thiệu cảm biến sinh học cholesterol 2.3.1 Cảm biến sinh học (Biosensor) 2.3.1.1 Tổng quan cảm biến sinh học .9 2.3.1.2 Cấu tạo nguyên lý hoạt động cảm biến sinh học 2.3.2 Cảm biến sinh học cholesterol 10 2.3.2.1 Giới thiệu sợi nano Platin 11 2.3.2.2 Giới thiệu enzyme cholesterol oxydase 11 iv 2.3.3 Một số nghiên cứu ứng dụng cảm biến sinh học để định lƣợng cholesterol 13 2.3.4 Cố định enzyme COx vào sợi nano Pt cảm biến sinh học cholesterol 14 2.3.4.1 Kỹ thuật tạo lớp đơn tự ghép (self-assembled monolayer - SAM) .14 2.3.4.2 Nguyên lý hình thành lớp đơn tự ghép 14 2.3.4.3 Đặc tính lớp đơn tự ghép .15 2.3.5 Kỹ thuật qt dòng tuần hồn (CV – cyclic voltammetry) 16 2.3.5.1 Giới thiệu kỹ thuật qt dòng tuần hồn .16 2.3.5.2 Nguyên lý tiến hành .17 2.3.5.3 Đồ thị qt dòng tuần hồn 18 2.3.5.4 Đặc điểm qt dòng tuần hồn tham số đặc trƣng 19 2.3.6 Hệ thiết bị phân tích điện hóa đa Autolab .19 Chƣơng VẬT LIỆU VÀ PHƢƠNG PHÁP NGHIÊN CỨU 21 3.1 Thời gian địa điểm nghiên cứu .21 3.2 Vật liệu hóa chất nghiên cứu 21 3.2.1 Vật liệu nghiên cứu 21 3.2.2 Hóa chất 21 3.2.3 Thiết bị 22 3.3 Phƣơng pháp nghiên cứu .22 3.3.1 Chuẩn bị hóa chất vật liệu thí nghiệm 22 3.3.1.1 Chuẩn bị hóa chất thí nghiệm 22 3.3.1.2 Làm bề mặt chip 23 3.3.2 Quá trình cố định enzyme cholesterol oxydase 23 3.3.3 Quá trình phân tích xử lý số liệu 25 Chƣơng KẾT QUẢ VÀ THẢO LUẬN 26 4.1 Kết trình làm bề mặt chip 26 4.1.1 Kết trình khử bề mặt chip dung dịch H2SO4 .26 4.1.2 Kết trình khảo sát chip dung dịch K3Fe(CN)6 26 4.2 Kết trình biến đổi bề mặt chip gắn enzyme 27 4.2.1 Khảo sát trình gắn cysteamine 27 4.2.2 Khảo sát trình gắn glutaraldehyde .30 4.2.3 Quá trình gắn enzyme cholesterol oxydase lên sợi nano Pt .33 v 4.2.4 Kết định lƣợng cholesterol dung dịch PBS 35 Chƣơng KẾT LUẬN VÀ ĐỀ NGHỊ 38 5.1 Kết luận .38 5.2 Đề nghị 38 TÀI LIỆU THAM KHẢO 39 PHỤ LỤC vi DANH SÁCH CÁC CHỮ VIẾT TẮT A Ampe BCO Brevibacterium sterolicum CA Cysteamine Ccholesterol Nồng độ cholesterol CE Counter Electrode COx Cholesterol Oxydase Ctv Cộng tác viên CV Cyclic Voltammetry DI De-ion FAD Flavin Adenine Dinucleotide GAD Glutaraldehyde HDL High Density Lipoprotein Ipeak Cƣờng độ dòng thu nhận LDL Low Density Lipoprotein O Chất oxy hóa PBS Phosphate Buffer Saline Pt Platin R Chất khử RE Reference Electrode SAM Self-Assembled Monolayer SCO Streptomyces hygroscopicus SEM Scanning Electron Microscope WE Woking Electrode V Volt vii DANH SÁCH CÁC BẢNG Trang Bảng 3.1 Thông số kỹ thuật chip sinh học sợi nano Platin 21 Bảng 3.2 Các loại dung mơi dùng để pha hóa chất thí nghiệm 22 Bảng 4.3 Cƣờng độ peak tổng hợp theo nồng độ cholesterol dung dịch cholesterol nồng độ 0,8 – mM, quét +0,625 V, tốc độ quét 0,1 V/s, điện khảo sát từ -0,1 đến +0,6 V 36 viii Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch H2SO4 0,01M x y x y x y x y x y x y -0.2127 5.97E-06 -0.1471 4.46E-06 -0.0815 3.54E-06 -0.0159 2.94E-06 -0.0479 2.31E-06 -0.1135 1.88E-06 -0.2112 5.94E-06 -0.1456 4.43E-06 -0.08 3.53E-06 -0.0143 2.93E-06 -0.0494 2.30E-06 -0.1151 1.87E-06 -0.2097 5.91E-06 -0.144 4.40E-06 -0.0784 3.51E-06 -0.0128 2.92E-06 -0.051 2.29E-06 -0.1166 1.86E-06 -0.2081 5.88E-06 -0.1425 4.37E-06 -0.0769 3.50E-06 -0.0113 2.91E-06 -0.0525 2.28E-06 -0.1181 1.85E-06 -0.2066 5.84E-06 -0.141 4.34E-06 -0.0754 3.49E-06 -0.0098 2.90E-06 -0.054 2.27E-06 -0.1196 1.85E-06 -0.2051 5.81E-06 -0.1395 4.32E-06 -0.0739 3.47E-06 -0.0082 2.88E-06 -0.0555 2.26E-06 -0.1212 1.84E-06 -0.2036 5.77E-06 -0.1379 4.29E-06 -0.0723 3.45E-06 -0.0067 2.87E-06 -0.0571 2.25E-06 -0.1227 1.83E-06 -0.202 5.73E-06 -0.1364 4.26E-06 -0.0708 3.44E-06 -0.0052 2.86E-06 -0.0586 2.23E-06 -0.1242 1.82E-06 -0.2005 5.69E-06 -0.1349 4.24E-06 -0.0693 3.43E-06 -0.0037 2.84E-06 -0.0601 2.22E-06 -0.1257 1.81E-06 -0.199 5.66E-06 -0.1334 4.21E-06 -0.0678 3.41E-06 -0.0021 2.83E-06 -0.0617 2.21E-06 -0.1273 1.80E-06 -0.1974 5.62E-06 -0.1318 4.19E-06 -0.0662 3.40E-06 -0.0006 2.81E-06 -0.0632 2.20E-06 -0.1288 1.79E-06 -0.1959 5.58E-06 -0.1303 4.16E-06 -0.0647 3.38E-06 0.0009 2.79E-06 -0.0647 2.18E-06 -0.1303 1.79E-06 -0.1944 5.54E-06 -0.1288 4.14E-06 -0.0632 3.37E-06 -0.0006 2.78E-06 -0.0662 2.17E-06 -0.1318 1.78E-06 -0.1929 5.50E-06 -0.1273 4.12E-06 -0.0617 3.35E-06 -0.0021 2.76E-06 -0.0678 2.16E-06 -0.1334 1.77E-06 -0.1913 5.47E-06 -0.1257 4.10E-06 -0.0601 3.33E-06 -0.0037 2.74E-06 -0.0693 2.15E-06 -0.1349 1.76E-06 -0.1898 5.43E-06 -0.1242 4.07E-06 -0.0586 3.32E-06 -0.0052 2.72E-06 -0.0708 2.14E-06 -0.1364 1.76E-06 -0.1883 5.39E-06 -0.1227 4.05E-06 -0.0571 3.31E-06 -0.0067 2.70E-06 -0.0723 2.13E-06 -0.1379 1.75E-06 -0.1868 5.35E-06 -0.1212 4.03E-06 -0.0555 3.29E-06 -0.0082 2.68E-06 -0.0739 2.12E-06 -0.1395 1.74E-06 -0.1852 5.31E-06 -0.1196 4.00E-06 -0.054 3.28E-06 -0.0098 2.67E-06 -0.0754 2.11E-06 -0.141 -0.1837 5.28E-06 -0.1181 3.98E-06 -0.0525 3.26E-06 -0.0113 2.65E-06 -0.0769 2.10E-06 -0.1425 1.73E-06 -0.1822 5.24E-06 -0.1166 3.96E-06 -0.051 3.25E-06 -0.0128 2.63E-06 -0.0784 2.09E-06 -0.144 -0.1807 5.20E-06 -0.1151 3.94E-06 -0.0494 3.23E-06 -0.0143 2.62E-06 -0.08 2.08E-06 -0.1456 1.72E-06 -0.1791 5.16E-06 -0.1135 3.92E-06 -0.0479 3.22E-06 -0.0159 2.60E-06 -0.0815 2.07E-06 -0.1471 1.71E-06 -0.1776 5.12E-06 -0.112 3.90E-06 -0.0464 3.21E-06 -0.0174 2.58E-06 -0.083 2.06E-06 -0.1486 1.71E-06 -0.1761 5.08E-06 -0.1105 3.88E-06 -0.0449 3.19E-06 -0.0189 2.57E-06 -0.0845 2.05E-06 -0.1501 1.70E-06 -0.1746 5.05E-06 -0.1089 3.86E-06 -0.0433 3.18E-06 -0.0205 2.56E-06 -0.0861 2.04E-06 -0.1517 1.69E-06 -0.173 1.74E-06 1.72E-06 5.01E-06 -0.1074 3.84E-06 -0.0418 3.17E-06 -0.022 2.54E-06 -0.0876 2.03E-06 -0.1532 1.69E-06 -0.1715 4.97E-06 -0.1059 3.82E-06 -0.0403 3.16E-06 -0.0235 2.53E-06 -0.0891 2.02E-06 -0.1547 1.68E-06 -0.17 4.94E-06 -0.1044 3.80E-06 -0.0388 3.14E-06 -0.025 2.51E-06 -0.0906 2.01E-06 -0.1563 1.67E-06 -0.1685 4.90E-06 -0.1028 3.79E-06 -0.0372 3.13E-06 -0.0266 2.50E-06 -0.0922 2.00E-06 -0.1578 1.66E-06 -0.1669 4.87E-06 -0.1013 3.77E-06 -0.0357 3.12E-06 -0.0281 2.48E-06 -0.0937 1.99E-06 -0.1593 1.66E-06 -0.1654 4.83E-06 -0.0998 3.75E-06 -0.0342 3.10E-06 -0.0296 2.47E-06 -0.0952 1.98E-06 -0.1608 1.65E-06 -0.1639 4.80E-06 -0.0983 3.73E-06 -0.0327 3.09E-06 -0.0311 2.46E-06 -0.0967 1.97E-06 -0.1624 1.64E-06 -0.1624 4.77E-06 -0.0967 3.71E-06 -0.0311 3.07E-06 -0.0327 2.44E-06 -0.0983 1.96E-06 -0.1639 1.63E-06 -0.1608 4.73E-06 -0.0952 3.70E-06 -0.0296 3.06E-06 -0.0342 2.43E-06 -0.0998 1.95E-06 -0.1654 1.63E-06 -0.1593 4.70E-06 -0.0937 3.68E-06 -0.0281 3.04E-06 -0.0357 2.41E-06 -0.1013 1.94E-06 -0.1669 1.62E-06 -0.1578 4.67E-06 -0.0922 3.66E-06 -0.0266 3.03E-06 -0.0372 2.40E-06 -0.1028 1.93E-06 -0.1685 1.62E-06 -0.1563 4.64E-06 -0.0906 3.64E-06 -0.025 3.02E-06 -0.0388 2.39E-06 -0.1044 1.92E-06 -0.17 -0.1547 4.60E-06 -0.0891 3.63E-06 -0.0235 3.00E-06 -0.0403 2.37E-06 -0.1059 1.91E-06 -0.1715 1.60E-06 -0.1532 4.57E-06 -0.0876 3.61E-06 -0.022 2.99E-06 -0.0418 2.36E-06 -0.1074 1.91E-06 -0.173 -0.1517 4.55E-06 -0.0861 3.59E-06 -0.0205 2.98E-06 -0.0433 2.35E-06 -0.1089 1.90E-06 -0.1746 1.59E-06 -0.1501 4.51E-06 -0.0845 3.58E-06 -0.0189 2.97E-06 -0.0449 2.34E-06 -0.1105 1.89E-06 -0.1761 1.59E-06 -0.1486 4.48E-06 -0.083 -0.0174 2.95E-06 -0.0464 2.33E-06 -0.112 -0.1776 1.58E-06 3.56E-06 1.88E-06 1.61E-06 1.60E-06 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch H2SO4 0,01M x y x y x y x y x y x y -0.1791 1.58E-06 -0.2448 1.24E-06 -0.3104 5.89E-07 -0.376 -2.46E-06 -0.4416 -9.26E-06 -0.5072 -1.71E-05 -0.1807 1.57E-06 -0.2463 1.23E-06 -0.3119 5.61E-07 -0.3775 -2.58E-06 -0.4431 -9.44E-06 -0.5087 -1.72E-05 -0.1822 1.57E-06 -0.2478 1.22E-06 -0.3134 5.33E-07 -0.379 -2.71E-06 -0.4446 -9.62E-06 -0.5103 -1.74E-05 -0.1837 1.56E-06 -0.2493 1.21E-06 -0.3149 5.04E-07 -0.3806 -2.84E-06 -0.4462 -9.80E-06 -0.5118 -1.76E-05 -0.1852 1.55E-06 -0.2509 1.20E-06 -0.3165 4.72E-07 -0.3821 -2.97E-06 -0.4477 -9.98E-06 -0.5133 -1.78E-05 -0.1868 1.54E-06 -0.2524 1.19E-06 -0.318 4.38E-07 -0.3836 -3.10E-06 -0.4492 -1.02E-05 -0.5148 -1.79E-05 -0.1883 1.54E-06 -0.2539 1.18E-06 -0.3195 4.04E-07 -0.3851 -3.24E-06 -0.4507 -1.03E-05 -0.5164 -1.81E-05 -0.1898 1.53E-06 -0.2554 1.17E-06 -0.321 3.69E-07 -0.3867 -3.38E-06 -0.4523 -1.05E-05 -0.5179 -1.83E-05 -0.1913 1.52E-06 -0.257 1.15E-06 -0.3226 3.31E-07 -0.3882 -3.52E-06 -0.4538 -1.07E-05 -0.5194 -1.84E-05 -0.1929 1.52E-06 -0.2585 1.14E-06 -0.3241 2.90E-07 -0.3897 -3.66E-06 -0.4553 -1.09E-05 -0.5209 -1.86E-05 -0.1944 1.51E-06 -0.26 1.13E-06 -0.3256 2.49E-07 -0.3912 -3.80E-06 -0.4568 -1.11E-05 -0.5225 -1.88E-05 -0.1959 1.50E-06 -0.2615 1.12E-06 -0.3271 2.07E-07 -0.3928 -3.95E-06 -0.4584 -1.13E-05 -0.524 -0.1974 1.49E-06 -0.2631 1.11E-06 -0.3287 1.61E-07 -0.3943 -4.09E-06 -0.4599 -1.14E-05 -0.5255 -1.91E-05 -0.199 1.49E-06 -0.2646 1.10E-06 -0.3302 1.13E-07 -0.3958 -4.24E-06 -0.4614 -1.16E-05 -0.527 -0.2005 1.48E-06 -0.2661 1.09E-06 -0.3317 6.33E-08 -0.3973 -4.39E-06 -0.463 -1.18E-05 -0.5286 -1.94E-05 -0.202 1.47E-06 -0.2676 1.08E-06 -0.3333 1.29E-08 -0.3989 -4.54E-06 -0.4645 -1.20E-05 -0.5301 -1.96E-05 -0.2036 1.47E-06 -0.2692 1.07E-06 -0.3348 -4.06E-08 -0.4004 -4.70E-06 -0.466 -1.22E-05 -0.5316 -1.98E-05 -0.2051 1.46E-06 -0.2707 1.05E-06 -0.3363 -9.80E-08 -0.4019 -4.85E-06 -0.4675 -1.24E-05 -0.5331 -1.99E-05 -0.2066 1.45E-06 -0.2722 1.04E-06 -0.3378 -1.56E-07 -0.4034 -5.01E-06 -0.4691 -1.25E-05 -0.5347 -2.01E-05 -0.2081 1.45E-06 -0.2737 1.03E-06 -0.3394 -2.16E-07 -0.405 -5.17E-06 -0.4706 -1.27E-05 -0.5362 -2.03E-05 -0.2097 1.44E-06 -0.2753 1.02E-06 -0.3409 -2.78E-07 -0.4065 -5.33E-06 -0.4721 -1.29E-05 -0.5377 -2.04E-05 -0.2112 1.43E-06 -0.2768 1.00E-06 -0.3424 -3.45E-07 -0.408 -5.49E-06 -0.4736 -1.31E-05 -0.5392 -2.06E-05 -0.2127 1.43E-06 -0.2783 9.89E-07 -0.3439 -4.14E-07 -0.4095 -5.65E-06 -0.4752 -1.33E-05 -0.5408 -2.07E-05 -0.2142 1.42E-06 -0.2798 9.75E-07 -0.3455 -4.84E-07 -0.4111 -5.81E-06 -0.4767 -1.35E-05 -0.5423 -2.09E-05 -0.2158 1.41E-06 -0.2814 9.60E-07 -0.347 -5.56E-07 -0.4126 -5.97E-06 -0.4782 -1.36E-05 -0.5438 -2.10E-05 -0.2173 1.40E-06 -0.2829 9.46E-07 -0.3485 -6.33E-07 -0.4141 -6.14E-06 -0.4797 -1.38E-05 -0.5453 -2.12E-05 -0.2188 1.39E-06 -0.2844 9.29E-07 -0.35 -7.13E-07 -0.4156 -6.31E-06 -0.4813 -1.40E-05 -0.5469 -2.14E-05 -0.2203 1.38E-06 -0.2859 9.13E-07 -0.3516 -7.94E-07 -0.4172 -6.47E-06 -0.4828 -1.42E-05 -0.5484 -2.15E-05 -0.2219 1.37E-06 -0.2875 8.98E-07 -0.3531 -8.77E-07 -0.4187 -6.64E-06 -0.4843 -1.44E-05 -0.5499 -2.17E-05 -0.2234 1.37E-06 -0.289 8.83E-07 -0.3546 -9.64E-07 -0.4202 -6.81E-06 -0.4858 -1.46E-05 -0.5515 -2.18E-05 -0.2249 1.36E-06 -0.2905 8.65E-07 -0.3561 -1.06E-06 -0.4218 -6.98E-06 -0.4874 -1.47E-05 -0.553 -0.2264 1.35E-06 -0.2921 8.47E-07 -0.3577 -1.15E-06 -0.4233 -7.15E-06 -0.4889 -1.49E-05 -0.5545 -2.21E-05 -0.228 1.34E-06 -0.2936 8.30E-07 -0.3592 -1.24E-06 -0.4248 -7.32E-06 -0.4904 -1.51E-05 -0.556 -0.2295 1.33E-06 -0.2951 8.13E-07 -0.3607 -1.34E-06 -0.4263 -7.50E-06 -0.4919 -1.53E-05 -0.5576 -2.24E-05 -0.231 -1.89E-05 -1.93E-05 -2.20E-05 -2.22E-05 1.32E-06 -0.2966 7.94E-07 -0.3622 -1.44E-06 -0.4279 -7.67E-06 -0.4935 -1.55E-05 -0.5591 -2.25E-05 -0.2325 1.31E-06 -0.2982 7.73E-07 -0.3638 -1.54E-06 -0.4294 -7.84E-06 -0.495 -1.56E-05 -0.5606 -2.27E-05 -0.2341 1.30E-06 -0.2997 7.53E-07 -0.3653 -1.65E-06 -0.4309 -8.02E-06 -0.4965 -1.58E-05 -0.5621 -2.28E-05 -0.2356 1.30E-06 -0.3012 7.33E-07 -0.3668 -1.76E-06 -0.4324 -8.20E-06 -0.498 -1.60E-05 -0.5637 -2.29E-05 -0.2371 1.29E-06 -0.3027 7.12E-07 -0.3683 -1.87E-06 -0.434 -8.37E-06 -0.4996 -1.62E-05 -0.5652 -2.31E-05 -0.2386 1.28E-06 -0.3043 6.88E-07 -0.3699 -1.98E-06 -0.4355 -8.55E-06 -0.5011 -1.63E-05 -0.5667 -2.32E-05 -0.2402 1.27E-06 -0.3058 6.65E-07 -0.3714 -2.10E-06 -0.437 -8.72E-06 -0.5026 -1.65E-05 -0.5682 -2.34E-05 -0.2417 1.26E-06 -0.3073 6.41E-07 -0.3729 -2.21E-06 -0.4385 -8.90E-06 -0.5042 -1.67E-05 -0.5698 -2.35E-05 -0.2432 1.25E-06 -0.3088 6.17E-07 -0.3745 -2.33E-06 -0.4401 -9.08E-06 -0.5057 -1.69E-05 -0.5713 -2.36E-05 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch H2SO4 0,01M x y x y x y x y x y x y -0.5728 -2.37E-05 -0.6384 -2.72E-05 -0.704 -2.61E-05 -0.7697 -2.43E-05 -0.8353 -2.30E-05 -0.9009 -2.22E-05 -0.5743 -2.39E-05 -0.64 -2.72E-05 -0.7056 -2.61E-05 -0.7712 -2.43E-05 -0.8368 -2.30E-05 -0.9024 -2.22E-05 -0.5759 -2.40E-05 -0.6415 -2.72E-05 -0.7071 -2.60E-05 -0.7727 -2.42E-05 -0.8383 -2.30E-05 -0.9039 -2.21E-05 -0.5774 -2.41E-05 -0.643 -2.72E-05 -0.7086 -2.60E-05 -0.7742 -2.42E-05 -0.8398 -2.30E-05 -0.9055 -2.21E-05 -0.5789 -2.42E-05 -0.6445 -2.72E-05 -0.7101 -2.59E-05 -0.7758 -2.42E-05 -0.8414 -2.29E-05 -0.907 -0.5804 -2.44E-05 -0.6461 -2.72E-05 -0.7117 -2.59E-05 -0.7773 -2.41E-05 -0.8429 -2.29E-05 -0.9085 -2.21E-05 -0.582 -2.21E-05 -2.45E-05 -0.6476 -2.73E-05 -0.7132 -2.58E-05 -0.7788 -2.41E-05 -0.8444 -2.29E-05 -0.91 -0.5835 -2.46E-05 -0.6491 -2.73E-05 -0.7147 -2.58E-05 -0.7803 -2.41E-05 -0.8459 -2.29E-05 -0.9116 -2.21E-05 -2.21E-05 -0.585 -2.47E-05 -0.6506 -2.73E-05 -0.7162 -2.57E-05 -0.7819 -2.40E-05 -0.8475 -2.29E-05 -0.9131 -2.21E-05 -0.5865 -2.48E-05 -0.6522 -2.73E-05 -0.7178 -2.57E-05 -0.7834 -2.40E-05 -0.849 -2.28E-05 -0.9146 -2.20E-05 -0.5881 -2.49E-05 -0.6537 -2.73E-05 -0.7193 -2.56E-05 -0.7849 -2.40E-05 -0.8505 -2.28E-05 -0.9161 -2.20E-05 -0.5896 -2.50E-05 -0.6552 -2.73E-05 -0.7208 -2.56E-05 -0.7864 -2.39E-05 -0.8521 -2.28E-05 -0.9177 -2.20E-05 -0.5911 -2.51E-05 -0.6567 -2.72E-05 -0.7224 -2.56E-05 -0.788 -2.39E-05 -0.8536 -2.28E-05 -0.9192 -2.20E-05 -0.5927 -2.52E-05 -0.6583 -2.72E-05 -0.7239 -2.55E-05 -0.7895 -2.39E-05 -0.8551 -2.27E-05 -0.9207 -2.20E-05 -0.5942 -2.53E-05 -0.6598 -2.72E-05 -0.7254 -2.55E-05 -0.791 -2.38E-05 -0.8566 -2.27E-05 -0.9222 -2.20E-05 -0.5957 -2.54E-05 -0.6613 -2.72E-05 -0.7269 -2.54E-05 -0.7925 -2.38E-05 -0.8582 -2.27E-05 -0.9238 -2.20E-05 -0.5972 -2.55E-05 -0.6628 -2.72E-05 -0.7285 -2.54E-05 -0.7941 -2.38E-05 -0.8597 -2.27E-05 -0.9253 -2.19E-05 -0.5988 -2.56E-05 -0.6644 -2.72E-05 -0.73 -2.53E-05 -0.7956 -2.37E-05 -0.8612 -2.27E-05 -0.9268 -2.19E-05 -0.6003 -2.57E-05 -0.6659 -2.71E-05 -0.7315 -2.53E-05 -0.7971 -2.37E-05 -0.8627 -2.26E-05 -0.9283 -2.19E-05 -0.6018 -2.58E-05 -0.6674 -2.71E-05 -0.733 -2.52E-05 -0.7986 -2.37E-05 -0.8643 -2.26E-05 -0.9299 -2.19E-05 -0.6033 -2.59E-05 -0.6689 -2.71E-05 -0.7346 -2.52E-05 -0.8002 -2.37E-05 -0.8658 -2.26E-05 -0.9314 -2.19E-05 -0.6049 -2.60E-05 -0.6705 -2.71E-05 -0.7361 -2.52E-05 -0.8017 -2.36E-05 -0.8673 -2.26E-05 -0.9329 -2.19E-05 -0.6064 -2.61E-05 -0.672 -2.70E-05 -0.7376 -2.51E-05 -0.8032 -2.36E-05 -0.8688 -2.26E-05 -0.9344 -2.19E-05 -0.6079 -2.61E-05 -0.6735 -2.70E-05 -0.7391 -2.51E-05 -0.8047 -2.36E-05 -0.8704 -2.25E-05 -0.936 -0.6094 -2.62E-05 -0.675 -2.70E-05 -0.7407 -2.50E-05 -0.8063 -2.35E-05 -0.8719 -2.25E-05 -0.9375 -2.18E-05 -0.611 -2.63E-05 -0.6766 -2.69E-05 -0.7422 -2.50E-05 -0.8078 -2.35E-05 -0.8734 -2.25E-05 -0.939 -0.6125 -2.64E-05 -0.6781 -2.69E-05 -0.7437 -2.49E-05 -0.8093 -2.35E-05 -0.8749 -2.25E-05 -0.9406 -2.18E-05 -0.614 -2.64E-05 -0.6796 -2.68E-05 -0.7452 -2.49E-05 -0.8109 -2.35E-05 -0.8765 -2.25E-05 -0.9421 -2.18E-05 -0.6155 -2.65E-05 -0.6812 -2.68E-05 -0.7468 -2.49E-05 -0.8124 -2.34E-05 -0.878 -2.24E-05 -0.9436 -2.18E-05 -0.6171 -2.66E-05 -0.6827 -2.68E-05 -0.7483 -2.48E-05 -0.8139 -2.34E-05 -0.8795 -2.24E-05 -0.9451 -2.18E-05 -0.6186 -2.66E-05 -0.6842 -2.67E-05 -0.7498 -2.48E-05 -0.8154 -2.34E-05 -0.881 -2.24E-05 -0.9467 -2.18E-05 -0.6201 -2.67E-05 -0.6857 -2.67E-05 -0.7513 -2.47E-05 -0.817 -2.33E-05 -0.8826 -2.24E-05 -0.9482 -2.17E-05 -0.6216 -2.67E-05 -0.6873 -2.66E-05 -0.7529 -2.47E-05 -0.8185 -2.33E-05 -0.8841 -2.24E-05 -0.9497 -2.17E-05 -0.6232 -2.68E-05 -0.6888 -2.66E-05 -0.7544 -2.47E-05 -0.82 -2.33E-05 -0.8856 -2.24E-05 -0.9512 -2.17E-05 -0.6247 -2.68E-05 -0.6903 -2.65E-05 -0.7559 -2.46E-05 -0.8215 -2.33E-05 -0.8871 -2.23E-05 -0.9528 -2.17E-05 -0.6262 -2.69E-05 -0.6918 -2.65E-05 -0.7574 -2.46E-05 -0.8231 -2.32E-05 -0.8887 -2.23E-05 -0.9543 -2.17E-05 -0.6277 -2.69E-05 -0.6934 -2.65E-05 -0.759 -2.46E-05 -0.8246 -2.32E-05 -0.8902 -2.23E-05 -0.9558 -2.17E-05 -0.6293 -2.70E-05 -0.6949 -2.64E-05 -0.7605 -2.45E-05 -0.8261 -2.32E-05 -0.8917 -2.23E-05 -0.9573 -2.17E-05 -0.6308 -2.70E-05 -0.6964 -2.64E-05 -0.762 -2.45E-05 -0.8276 -2.32E-05 -0.8932 -2.23E-05 -0.9589 -2.17E-05 -0.6323 -2.71E-05 -0.6979 -2.63E-05 -0.7635 -2.44E-05 -0.8292 -2.31E-05 -0.8948 -2.22E-05 -0.9604 -2.16E-05 -0.6339 -2.71E-05 -0.6995 -2.63E-05 -0.7651 -2.44E-05 -0.8307 -2.31E-05 -0.8963 -2.22E-05 -0.9619 -2.16E-05 -0.6354 -2.71E-05 -0.701 -2.62E-05 -0.7666 -2.44E-05 -0.8322 -2.31E-05 -0.8978 -2.22E-05 -0.9634 -2.16E-05 -0.6369 -2.71E-05 -0.7025 -2.62E-05 -0.7681 -2.43E-05 -0.8337 -2.31E-05 -0.8994 -2.22E-05 -0.965 -2.19E-05 -2.18E-05 -2.16E-05 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch H2SO4 0,01M x y x y x y x y x y x y -0.9665 -2.16E-05 -0.9726 -2.16E-05 -0.9787 -2.15E-05 -0.9848 -2.15E-05 -0.9909 -2.14E-05 -0.997 -2.14E-05 -0.968 -2.16E-05 -0.9741 -2.15E-05 -0.9802 -2.15E-05 -0.9863 -2.15E-05 -0.9924 -2.14E-05 -0.9985 -2.14E-05 -0.9695 -2.16E-05 -0.9756 -2.15E-05 -0.9818 -2.15E-05 -0.9879 -2.15E-05 -0.994 -0.9711 -2.16E-05 -0.9772 -2.15E-05 -0.9833 -2.15E-05 -0.9894 -2.14E-05 -0.9955 -2.14E-05 -2.14E-05 Bảng Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch K3Fe(CN)6 mM, pH 4,0 với x: quét (V), y: cƣờng độ dòng (A) x y x y x y x y x y x y 0.6 2.61E-08 0.5481 1.98E-08 0.4962 1.55E-08 0.4443 1.18E-08 0.3925 9.59E-09 0.3406 7.18E-09 0.5984 3.71E-08 0.5466 1.96E-08 0.4947 1.55E-08 0.4428 1.16E-08 0.3909 9.41E-09 0.3391 6.90E-09 0.5969 2.94E-08 0.545 1.94E-08 0.4932 1.56E-08 0.4413 1.16E-08 0.3894 9.23E-09 0.3375 6.59E-09 0.5954 2.90E-08 0.5435 1.92E-08 0.4916 1.56E-08 0.4398 1.15E-08 0.3879 9.07E-09 0.336 6.25E-09 0.5939 2.76E-08 0.542 1.89E-08 0.4901 1.56E-08 0.4382 1.15E-08 0.3864 8.93E-09 0.3345 5.89E-09 0.5923 2.63E-08 0.5405 1.87E-08 0.4886 1.55E-08 0.4367 1.15E-08 0.3848 8.82E-09 0.3329 5.52E-09 0.5908 2.59E-08 0.5389 1.84E-08 0.4871 1.54E-08 0.4352 1.16E-08 0.3833 8.73E-09 0.3314 5.15E-09 0.5893 2.58E-08 0.5374 1.82E-08 0.4855 1.52E-08 0.4337 1.16E-08 0.3818 8.67E-09 0.3299 4.80E-09 0.5878 2.61E-08 0.5359 1.79E-08 0.484 1.50E-08 0.4321 1.17E-08 0.3802 8.64E-09 0.3284 4.46E-09 0.5862 2.64E-08 0.5344 1.77E-08 0.4825 1.47E-08 0.4306 1.17E-08 0.3787 8.63E-09 0.3268 4.15E-09 0.5847 2.62E-08 0.5328 1.76E-08 0.481 1.45E-08 0.4291 1.17E-08 0.3772 8.64E-09 0.3253 3.87E-09 0.5832 2.64E-08 0.5313 1.74E-08 0.4794 1.42E-08 0.4276 1.17E-08 0.3757 8.67E-09 0.3238 3.63E-09 0.5817 2.59E-08 0.5298 1.73E-08 0.4779 1.40E-08 0.426 1.17E-08 0.3741 8.69E-09 0.3223 3.42E-09 0.5801 2.63E-08 0.5283 1.72E-08 0.4764 1.38E-08 0.4245 1.16E-08 0.3726 8.71E-09 0.3207 3.24E-09 0.5786 2.62E-08 0.5267 1.72E-08 0.4749 1.36E-08 0.423 1.16E-08 0.3711 8.72E-09 0.3192 3.07E-09 0.5771 2.61E-08 0.5252 1.71E-08 0.4733 1.34E-08 0.4214 1.15E-08 0.3696 8.70E-09 0.3177 2.92E-09 0.5756 2.60E-08 0.5237 1.71E-08 0.4718 1.33E-08 0.4199 1.14E-08 0.368 8.67E-09 0.3162 2.75E-09 0.574 2.57E-08 0.5222 1.71E-08 0.4703 1.33E-08 0.4184 1.13E-08 0.3665 8.61E-09 0.3146 2.57E-09 0.5725 2.53E-08 0.5206 1.70E-08 0.4688 1.32E-08 0.4169 1.12E-08 0.365 8.55E-09 0.3131 2.34E-09 0.571 2.48E-08 0.5191 1.69E-08 0.4672 1.32E-08 0.4153 1.11E-08 0.3635 8.47E-09 0.3116 2.07E-09 0.5695 2.43E-08 0.5176 1.68E-08 0.4657 1.33E-08 0.4138 1.10E-08 0.3619 8.39E-09 0.3101 1.75E-09 0.5679 2.38E-08 0.5161 1.66E-08 0.4642 1.33E-08 0.4123 1.09E-08 0.3604 8.31E-09 0.3085 1.37E-09 0.5664 2.33E-08 0.5145 1.65E-08 0.4626 1.33E-08 0.4108 1.08E-08 0.3589 8.24E-09 0.307 9.34E-10 0.5649 2.28E-08 0.513 1.63E-08 0.4611 1.33E-08 0.4092 1.08E-08 0.3574 8.17E-09 0.3055 4.57E-10 0.5634 2.23E-08 0.5115 1.61E-08 0.4596 1.33E-08 0.4077 1.07E-08 0.3558 8.11E-09 0.304 -4.94E-11 0.5618 2.18E-08 0.5099 1.59E-08 0.4581 1.33E-08 0.4062 1.07E-08 0.3543 8.06E-09 0.3024 -5.80E-10 0.5603 2.14E-08 0.5084 1.57E-08 0.4565 1.32E-08 0.4047 1.06E-08 0.3528 8.02E-09 0.3009 -1.13E-09 0.5588 2.11E-08 0.5069 1.55E-08 0.455 1.30E-08 0.4031 1.06E-08 0.3513 7.99E-09 0.2994 -1.69E-09 0.5573 2.08E-08 0.5054 1.54E-08 0.4535 1.28E-08 0.4016 1.05E-08 0.3497 7.95E-09 0.2979 -2.24E-09 0.5557 2.06E-08 0.5038 1.54E-08 0.452 1.27E-08 0.4001 1.04E-08 0.3482 7.90E-09 0.2963 -2.78E-09 0.5542 2.04E-08 0.5023 1.53E-08 0.4504 1.25E-08 0.3986 1.03E-08 0.3467 7.83E-09 0.2948 -3.33E-09 0.5527 2.03E-08 0.5008 1.53E-08 0.4489 1.23E-08 0.397 1.01E-08 0.3452 7.73E-09 0.2933 -3.87E-09 0.5511 2.01E-08 0.4993 1.53E-08 0.4474 1.21E-08 0.3955 9.95E-09 0.3436 7.59E-09 0.2917 -4.39E-09 0.5496 2.00E-08 0.4977 1.54E-08 0.4459 1.19E-08 0.394 9.77E-09 0.3421 7.41E-09 0.2902 -4.90E-09 Bảng (tt) Dữ liệu đồ thị quét dòng tuần hoàn – CV chip Pt dung dịch K3Fe(CN)6 mM, pH 4,0 x y x y x y x y x y x y 0.2887 -5.42E-09 0.2231 -8.05E-08 0.1575 -2.19E-07 0.0919 -2.25E-07 0.0263 -2.02E-07 -0.0394 -1.87E-07 0.2872 -5.97E-09 0.2216 -8.36E-08 0.1559 -2.21E-07 0.0903 -2.25E-07 0.0247 -2.02E-07 -0.0409 -1.86E-07 0.2856 -6.54E-09 0.22 -8.69E-08 0.1544 -2.22E-07 0.0888 -2.24E-07 0.0232 -2.01E-07 -0.0424 -1.86E-07 0.2841 -7.16E-09 0.2185 -9.04E-08 0.1529 -2.24E-07 0.0873 -2.24E-07 0.0217 -2.01E-07 -0.044 -1.86E-07 0.2826 -7.85E-09 0.217 -9.40E-08 0.1514 -2.25E-07 0.0858 -2.23E-07 0.0201 -2.00E-07 -0.0455 -1.85E-07 0.2811 -8.61E-09 0.2155 -9.77E-08 0.1498 -2.27E-07 0.0842 -2.22E-07 0.0186 -2.00E-07 -0.047 -1.85E-07 0.2795 -9.45E-09 0.2139 -1.02E-07 0.1483 -2.28E-07 0.0827 -2.22E-07 0.0171 -1.99E-07 -0.0485 -1.85E-07 0.278 -1.04E-08 0.2124 -1.05E-07 0.1468 -2.29E-07 0.0812 -2.21E-07 0.0156 -1.99E-07 -0.0501 -1.84E-07 0.2765 -1.14E-08 0.2109 -1.09E-07 0.1453 -2.30E-07 0.0797 -2.20E-07 0.014 -1.99E-07 -0.0516 -1.84E-07 0.275 -1.25E-08 0.2094 -1.13E-07 0.1437 -2.31E-07 0.0781 -2.20E-07 0.0125 -1.98E-07 -0.0531 -1.84E-07 0.2734 -1.36E-08 0.2078 -1.17E-07 0.1422 -2.31E-07 0.0766 -2.19E-07 0.011 -1.98E-07 -0.0546 -1.84E-07 0.2719 -1.48E-08 0.2063 -1.21E-07 0.1407 -2.32E-07 0.0751 -2.18E-07 0.0095 -1.97E-07 -0.0562 -1.83E-07 0.2704 -1.60E-08 0.2048 -1.25E-07 0.1392 -2.33E-07 0.0736 -2.17E-07 0.0079 -1.97E-07 -0.0577 -1.83E-07 0.2689 -1.72E-08 0.2032 -1.29E-07 0.1376 -2.33E-07 0.072 -2.17E-07 0.0064 -1.97E-07 -0.0592 -1.83E-07 0.2673 -1.84E-08 0.2017 -1.32E-07 0.1361 -2.33E-07 0.0705 -2.16E-07 0.0049 -1.96E-07 -0.0607 -1.83E-07 0.2658 -1.97E-08 0.2002 -1.36E-07 0.1346 -2.33E-07 0.069 -2.16E-07 0.0034 -1.96E-07 -0.0623 -1.83E-07 0.2643 -2.09E-08 0.1987 -1.39E-07 0.1331 -2.34E-07 0.0674 -2.15E-07 0.0018 -1.95E-07 -0.0638 -1.82E-07 0.2628 -2.22E-08 0.1971 -1.43E-07 0.1315 -2.34E-07 0.0659 -2.15E-07 0.0003 -1.95E-07 -0.0653 -1.82E-07 0.2612 -2.35E-08 0.1956 -1.46E-07 0.13 -2.34E-07 0.0644 -2.14E-07 -0.0012 -1.95E-07 -0.0668 -1.82E-07 0.2597 -2.48E-08 0.1941 -1.50E-07 0.1285 -2.34E-07 0.0629 -2.14E-07 -0.0027 -1.94E-07 -0.0684 -1.81E-07 0.2582 -2.62E-08 0.1926 -1.54E-07 0.127 -2.34E-07 0.0613 -2.13E-07 -0.0043 -1.94E-07 -0.0699 -1.81E-07 0.2567 -2.77E-08 0.191 -1.57E-07 0.1254 -2.34E-07 0.0598 -2.13E-07 -0.0058 -1.93E-07 -0.0714 -1.81E-07 0.2551 -2.92E-08 0.1895 -1.61E-07 0.1239 -2.34E-07 0.0583 -2.12E-07 -0.0073 -1.93E-07 -0.0729 -1.81E-07 0.2536 -3.09E-08 0.188 -1.64E-07 0.1224 -2.34E-07 0.0568 -2.12E-07 -0.0089 -1.93E-07 -0.0745 -1.80E-07 0.2521 -3.27E-08 0.1865 -1.68E-07 0.1208 -2.34E-07 0.0552 -2.11E-07 -0.0104 -1.92E-07 -0.076 -1.80E-07 0.2505 -3.46E-08 0.1849 -1.72E-07 0.1193 -2.34E-07 0.0537 -2.11E-07 -0.0119 -1.92E-07 -0.0775 -1.80E-07 0.249 -3.66E-08 0.1834 -1.76E-07 0.1178 -2.33E-07 0.0522 -2.10E-07 -0.0134 -1.91E-07 -0.079 -1.80E-07 0.2475 -3.88E-08 0.1819 -1.79E-07 0.1163 -2.33E-07 0.0507 -2.09E-07 -0.015 -1.91E-07 -0.0806 -1.80E-07 0.246 -4.11E-08 0.1804 -1.83E-07 0.1147 -2.33E-07 0.0491 -2.09E-07 -0.0165 -1.90E-07 -0.0821 -1.80E-07 0.2444 -4.35E-08 0.1788 -1.86E-07 0.1132 -2.32E-07 0.0476 -2.08E-07 -0.018 -1.90E-07 -0.0836 -1.80E-07 0.2429 -4.59E-08 0.1773 -1.89E-07 0.1117 -2.32E-07 0.0461 -2.07E-07 -0.0195 -1.90E-07 -0.0851 -1.80E-07 0.2414 -4.85E-08 0.1758 -1.93E-07 0.1102 -2.31E-07 0.0446 -2.07E-07 -0.0211 -1.90E-07 -0.0867 -1.80E-07 0.2399 -5.10E-08 0.1743 -1.95E-07 0.1086 -2.30E-07 0.043 -2.06E-07 -0.0226 -1.89E-07 -0.0882 -1.80E-07 0.2383 -5.36E-08 0.1727 -1.98E-07 0.1071 -2.30E-07 0.0415 -2.06E-07 -0.0241 -1.89E-07 -0.0897 -1.80E-07 0.2368 -5.62E-08 0.1712 -2.01E-07 0.1056 -2.29E-07 0.04 -2.05E-07 -0.0256 -1.89E-07 -0.0913 -1.80E-07 0.2353 -5.88E-08 0.1697 -2.03E-07 0.1041 -2.29E-07 0.0385 -2.05E-07 -0.0272 -1.89E-07 -0.0928 -1.80E-07 0.2338 -6.14E-08 0.1682 -2.06E-07 0.1025 -2.28E-07 0.0369 -2.04E-07 -0.0287 -1.89E-07 -0.0943 -1.80E-07 0.2322 -6.40E-08 0.1666 -2.08E-07 0.101 -2.28E-07 0.0354 -2.04E-07 -0.0302 -1.88E-07 -0.0958 -1.80E-07 0.2307 -6.65E-08 0.1651 -2.10E-07 0.0995 -2.27E-07 0.0339 -2.04E-07 -0.0317 -1.88E-07 -0.0974 -1.79E-07 0.2292 -6.92E-08 0.1636 -2.12E-07 0.098 -2.27E-07 0.0324 -2.03E-07 -0.0333 -1.88E-07 -0.0989 -1.79E-07 0.2277 -7.19E-08 0.162 -2.14E-07 0.0964 -2.26E-07 0.0308 -2.03E-07 -0.0348 -1.88E-07 -0.1004 -1.79E-07 0.2261 -7.46E-08 0.1605 -2.15E-07 0.0949 -2.26E-07 0.0293 -2.03E-07 -0.0363 -1.87E-07 -0.1019 -1.79E-07 0.2246 -7.75E-08 0.159 -2.17E-07 0.0934 -2.26E-07 0.0278 -2.02E-07 -0.0378 -1.87E-07 -0.1035 -1.79E-07 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch K3Fe(CN)6 mM, pH 4,0 x y x y x y x y x y x y -0.105 -1.78E-07 -0.1706 -1.75E-07 -0.166 -1.63E-07 -0.1004 -1.55E-07 -0.0348 -1.47E-07 0.0308 -1.38E-07 -0.1065 -1.78E-07 -0.1721 -1.75E-07 -0.1645 -1.63E-07 -0.0989 -1.55E-07 -0.0333 -1.47E-07 0.0324 -1.38E-07 -0.108 -1.78E-07 -0.1736 -1.75E-07 -0.163 -1.62E-07 -0.0974 -1.55E-07 -0.0317 -1.47E-07 0.0339 -1.37E-07 -0.1096 -1.78E-07 -0.1752 -1.76E-07 -0.1614 -1.62E-07 -0.0958 -1.55E-07 -0.0302 -1.47E-07 0.0354 -1.37E-07 -0.1111 -1.78E-07 -0.1767 -1.76E-07 -0.1599 -1.62E-07 -0.0943 -1.54E-07 -0.0287 -1.46E-07 0.0369 -1.36E-07 -0.1126 -1.78E-07 -0.1782 -1.76E-07 -0.1584 -1.62E-07 -0.0928 -1.54E-07 -0.0272 -1.46E-07 0.0385 -1.36E-07 -0.1141 -1.78E-07 -0.1797 -1.76E-07 -0.1569 -1.61E-07 -0.0913 -1.54E-07 -0.0256 -1.46E-07 0.04 -1.36E-07 -0.1157 -1.78E-07 -0.1813 -1.76E-07 -0.1553 -1.61E-07 -0.0897 -1.53E-07 -0.0241 -1.46E-07 0.0415 -1.35E-07 -0.1172 -1.78E-07 -0.1828 -1.76E-07 -0.1538 -1.61E-07 -0.0882 -1.53E-07 -0.0226 -1.45E-07 0.043 -1.35E-07 -0.1187 -1.78E-07 -0.1843 -1.76E-07 -0.1523 -1.60E-07 -0.0867 -1.53E-07 -0.0211 -1.45E-07 0.0446 -1.35E-07 -0.1202 -1.78E-07 -0.1859 -1.76E-07 -0.1508 -1.60E-07 -0.0851 -1.52E-07 -0.0195 -1.45E-07 0.0461 -1.35E-07 -0.1218 -1.78E-07 -0.1874 -1.76E-07 -0.1492 -1.59E-07 -0.0836 -1.52E-07 -0.018 -1.45E-07 0.0476 -1.34E-07 -0.1233 -1.78E-07 -0.1889 -1.76E-07 -0.1477 -1.59E-07 -0.0821 -1.52E-07 -0.0165 -1.45E-07 0.0491 -1.34E-07 -0.1248 -1.78E-07 -0.1904 -1.76E-07 -0.1462 -1.58E-07 -0.0806 -1.52E-07 -0.015 -1.45E-07 0.0507 -1.34E-07 -0.1263 -1.78E-07 -0.192 -1.76E-07 -0.1447 -1.58E-07 -0.079 -1.52E-07 -0.0134 -1.45E-07 0.0522 -1.34E-07 -0.1279 -1.77E-07 -0.1935 -1.75E-07 -0.1431 -1.58E-07 -0.0775 -1.52E-07 -0.0119 -1.45E-07 0.0537 -1.33E-07 -0.1294 -1.77E-07 -0.195 -1.75E-07 -0.1416 -1.57E-07 -0.076 -1.52E-07 -0.0104 -1.45E-07 0.0552 -1.33E-07 -0.1309 -1.77E-07 -0.1965 -1.75E-07 -0.1401 -1.57E-07 -0.0745 -1.52E-07 -0.0089 -1.45E-07 0.0568 -1.33E-07 -0.1324 -1.77E-07 -0.1981 -1.74E-07 -0.1385 -1.57E-07 -0.0729 -1.52E-07 -0.0073 -1.45E-07 0.0583 -1.32E-07 -0.134 -1.77E-07 -0.1996 -1.74E-07 -0.137 -1.57E-07 -0.0714 -1.51E-07 -0.0058 -1.44E-07 0.0598 -1.32E-07 -0.1355 -1.77E-07 -0.2011 -1.73E-07 -0.1355 -1.57E-07 -0.0699 -1.51E-07 -0.0043 -1.44E-07 0.0613 -1.31E-07 -0.137 -1.76E-07 -0.1996 -1.73E-07 -0.134 -1.57E-07 -0.0684 -1.51E-07 -0.0027 -1.44E-07 0.0629 -1.31E-07 -0.1385 -1.76E-07 -0.1981 -1.73E-07 -0.1324 -1.57E-07 -0.0668 -1.51E-07 -0.0012 -1.44E-07 0.0644 -1.31E-07 -0.1401 -1.76E-07 -0.1965 -1.72E-07 -0.1309 -1.57E-07 -0.0653 -1.51E-07 0.0003 -1.43E-07 0.0659 -1.30E-07 -0.1416 -1.76E-07 -0.195 -1.72E-07 -0.1294 -1.57E-07 -0.0638 -1.51E-07 0.0018 -1.43E-07 0.0674 -1.30E-07 -0.1431 -1.76E-07 -0.1935 -1.71E-07 -0.1279 -1.57E-07 -0.0623 -1.50E-07 0.0034 -1.43E-07 0.069 -1.29E-07 -0.1447 -1.76E-07 -0.192 -1.71E-07 -0.1263 -1.57E-07 -0.0607 -1.50E-07 0.0049 -1.42E-07 0.0705 -1.29E-07 -0.1462 -1.76E-07 -0.1904 -1.70E-07 -0.1248 -1.56E-07 -0.0592 -1.50E-07 0.0064 -1.42E-07 0.072 -1.28E-07 -0.1477 -1.76E-07 -0.1889 -1.69E-07 -0.1233 -1.56E-07 -0.0577 -1.50E-07 0.0079 -1.42E-07 0.0736 -1.28E-07 -0.1492 -1.76E-07 -0.1874 -1.69E-07 -0.1218 -1.56E-07 -0.0562 -1.49E-07 0.0095 -1.42E-07 0.0751 -1.28E-07 -0.1508 -1.76E-07 -0.1859 -1.68E-07 -0.1202 -1.56E-07 -0.0546 -1.49E-07 0.011 -1.41E-07 0.0766 -1.27E-07 -0.1523 -1.76E-07 -0.1843 -1.68E-07 -0.1187 -1.56E-07 -0.0531 -1.49E-07 0.0125 -1.41E-07 0.0781 -1.27E-07 -0.1538 -1.76E-07 -0.1828 -1.67E-07 -0.1172 -1.55E-07 -0.0516 -1.49E-07 0.014 -1.41E-07 0.0797 -1.26E-07 -0.1553 -1.76E-07 -0.1813 -1.66E-07 -0.1157 -1.55E-07 -0.0501 -1.49E-07 0.0156 -1.41E-07 0.0812 -1.26E-07 -0.1569 -1.76E-07 -0.1797 -1.66E-07 -0.1141 -1.55E-07 -0.0485 -1.49E-07 0.0171 -1.41E-07 0.0827 -1.25E-07 -0.1584 -1.76E-07 -0.1782 -1.65E-07 -0.1126 -1.55E-07 -0.047 -1.49E-07 0.0186 -1.41E-07 0.0842 -1.25E-07 -0.1599 -1.76E-07 -0.1767 -1.65E-07 -0.1111 -1.55E-07 -0.0455 -1.49E-07 0.0201 -1.41E-07 0.0858 -1.24E-07 -0.1614 -1.76E-07 -0.1752 -1.64E-07 -0.1096 -1.55E-07 -0.044 -1.48E-07 0.0217 -1.40E-07 0.0873 -1.23E-07 -0.163 -1.76E-07 -0.1736 -1.64E-07 -0.108 -1.55E-07 -0.0424 -1.48E-07 0.0232 -1.40E-07 0.0888 -1.22E-07 -0.1645 -1.76E-07 -0.1721 -1.64E-07 -0.1065 -1.55E-07 -0.0409 -1.48E-07 0.0247 -1.40E-07 0.0903 -1.21E-07 -0.166 -1.75E-07 -0.1706 -1.63E-07 -0.105 -1.55E-07 -0.0394 -1.48E-07 0.0263 -1.39E-07 0.0919 -1.21E-07 -0.1675 -1.75E-07 -0.1691 -1.63E-07 -0.1035 -1.55E-07 -0.0378 -1.48E-07 0.0278 -1.39E-07 0.0934 -1.20E-07 -0.1691 -1.75E-07 -0.1675 -1.63E-07 -0.1019 -1.55E-07 -0.0363 -1.48E-07 0.0293 -1.38E-07 0.0949 -1.19E-07 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt dung dịch K3Fe(CN)6 mM, pH 4,0 x y x y x y x y x y x y 0.0964 -1.18E-07 0.162 -3.83E-08 0.2277 1.04E-07 0.2933 9.74E-08 0.3589 6.50E-08 0.4245 4.85E-08 0.098 -1.17E-07 0.1636 -3.51E-08 0.2292 1.06E-07 0.2948 9.65E-08 0.3604 6.46E-08 0.426 4.84E-08 0.0995 -1.16E-07 0.1651 -3.19E-08 0.2307 1.07E-07 0.2963 9.57E-08 0.3619 6.41E-08 0.4276 4.83E-08 0.101 -1.16E-07 0.1666 -2.87E-08 0.2322 1.09E-07 0.2979 9.48E-08 0.3635 6.37E-08 0.4291 4.81E-08 0.1025 -1.15E-07 0.1682 -2.54E-08 0.2338 1.10E-07 0.2994 9.40E-08 0.365 6.32E-08 0.4306 4.78E-08 0.1041 -1.14E-07 0.1697 -2.20E-08 0.2353 1.11E-07 0.3009 9.32E-08 0.3665 6.28E-08 0.4321 4.75E-08 0.1056 -1.13E-07 0.1712 -1.85E-08 0.2368 1.12E-07 0.3024 9.24E-08 0.368 6.22E-08 0.4337 4.72E-08 0.1071 -1.12E-07 0.1727 -1.49E-08 0.2383 1.13E-07 0.304 9.16E-08 0.3696 6.17E-08 0.4352 4.68E-08 0.1086 -1.11E-07 0.1743 -1.13E-08 0.2399 1.14E-07 0.3055 9.07E-08 0.3711 6.11E-08 0.4367 4.65E-08 0.1102 -1.10E-07 0.1758 -7.53E-09 0.2414 1.15E-07 0.307 8.99E-08 0.3726 6.05E-08 0.4382 4.61E-08 0.1117 -1.09E-07 0.1773 -3.69E-09 0.2429 1.15E-07 0.3085 8.90E-08 0.3741 5.98E-08 0.4398 4.57E-08 0.1132 -1.08E-07 0.1788 2.23E-10 0.2444 1.16E-07 0.3101 8.80E-08 0.3757 5.92E-08 0.4413 4.53E-08 0.1147 -1.07E-07 0.1804 4.20E-09 0.246 1.16E-07 0.3116 8.70E-08 0.3772 5.86E-08 0.4428 4.50E-08 0.1163 -1.06E-07 0.1819 8.22E-09 0.2475 1.16E-07 0.3131 8.61E-08 0.3787 5.81E-08 0.4443 4.46E-08 0.1178 -1.04E-07 0.1834 1.23E-08 0.249 1.16E-07 0.3146 8.51E-08 0.3802 5.75E-08 0.4459 4.43E-08 0.1193 -1.03E-07 0.1849 1.63E-08 0.2505 1.16E-07 0.3162 8.41E-08 0.3818 5.70E-08 0.4474 4.41E-08 0.1208 -1.01E-07 0.1865 2.02E-08 0.2521 1.16E-07 0.3177 8.32E-08 0.3833 5.65E-08 0.4489 4.38E-08 0.1224 -9.97E-08 0.188 2.42E-08 0.2536 1.16E-07 0.3192 8.23E-08 0.3848 5.61E-08 0.4504 4.36E-08 0.1239 -9.80E-08 0.1895 2.81E-08 0.2551 1.16E-07 0.3207 8.15E-08 0.3864 5.58E-08 0.452 4.34E-08 0.1254 -9.63E-08 0.191 3.19E-08 0.2567 1.15E-07 0.3223 8.07E-08 0.3879 5.55E-08 0.4535 4.32E-08 0.127 -9.46E-08 0.1926 3.56E-08 0.2582 1.15E-07 0.3238 7.99E-08 0.3894 5.52E-08 0.455 4.30E-08 0.1285 -9.28E-08 0.1941 3.93E-08 0.2597 1.15E-07 0.3253 7.93E-08 0.3909 5.49E-08 0.4565 4.28E-08 0.13 -9.11E-08 0.1956 4.29E-08 0.2612 1.14E-07 0.3268 7.86E-08 0.3925 5.47E-08 0.4581 4.26E-08 0.1315 -8.93E-08 0.1971 4.64E-08 0.2628 1.14E-07 0.3284 7.81E-08 0.394 5.44E-08 0.4596 4.23E-08 0.1331 -8.75E-08 0.1987 4.99E-08 0.2643 1.13E-07 0.3299 7.75E-08 0.3955 5.41E-08 0.4611 4.20E-08 0.1346 -8.56E-08 0.2002 5.34E-08 0.2658 1.13E-07 0.3314 7.70E-08 0.397 5.38E-08 0.4626 4.16E-08 0.1361 -8.37E-08 0.2017 5.68E-08 0.2673 1.12E-07 0.3329 7.64E-08 0.3986 5.35E-08 0.4642 4.13E-08 0.1376 -8.18E-08 0.2032 6.02E-08 0.2689 1.11E-07 0.3345 7.59E-08 0.4001 5.31E-08 0.4657 4.10E-08 0.1392 -7.97E-08 0.2048 6.35E-08 0.2704 1.11E-07 0.336 7.53E-08 0.4016 5.26E-08 0.4672 4.06E-08 0.1407 -7.76E-08 0.2063 6.69E-08 0.2719 1.10E-07 0.3375 7.47E-08 0.4031 5.22E-08 0.4688 4.03E-08 0.1422 -7.54E-08 0.2078 7.02E-08 0.2734 1.10E-07 0.3391 7.40E-08 0.4047 5.17E-08 0.4703 4.00E-08 0.1437 -7.30E-08 0.2094 7.35E-08 0.275 1.09E-07 0.3406 7.32E-08 0.4062 5.13E-08 0.4718 3.98E-08 0.1453 -7.06E-08 0.2109 7.67E-08 0.2765 1.08E-07 0.3421 7.25E-08 0.4077 5.08E-08 0.4733 3.95E-08 0.1468 -6.80E-08 0.2124 7.98E-08 0.278 1.07E-07 0.3436 7.17E-08 0.4092 5.04E-08 0.4749 3.94E-08 0.1483 -6.53E-08 0.2139 8.29E-08 0.2795 1.06E-07 0.3452 7.09E-08 0.4108 5.01E-08 0.4764 3.92E-08 0.1498 -6.25E-08 0.2155 8.58E-08 0.2811 1.05E-07 0.3467 7.01E-08 0.4123 4.97E-08 0.4779 3.91E-08 0.1514 -5.97E-08 0.217 8.87E-08 0.2826 1.04E-07 0.3482 6.93E-08 0.4138 4.95E-08 0.4794 3.91E-08 0.1529 -5.67E-08 0.2185 9.14E-08 0.2841 1.03E-07 0.3497 6.86E-08 0.4153 4.92E-08 0.481 3.90E-08 0.1544 -5.37E-08 0.22 9.39E-08 0.2856 1.02E-07 0.3513 6.78E-08 0.4169 4.90E-08 0.4825 3.90E-08 0.1559 -5.07E-08 0.2216 9.63E-08 0.2872 1.01E-07 0.3528 6.72E-08 0.4184 4.89E-08 0.484 3.90E-08 0.1575 -4.76E-08 0.2231 9.85E-08 0.2887 1.00E-07 0.3543 6.66E-08 0.4199 4.88E-08 0.4855 3.90E-08 0.159 -4.46E-08 0.2246 1.01E-07 0.2902 9.94E-08 0.3558 6.60E-08 0.4214 4.87E-08 0.4871 3.89E-08 0.1605 -4.14E-08 0.2261 1.03E-07 0.2917 9.84E-08 0.3574 6.55E-08 0.423 4.86E-08 0.4886 3.88E-08 Bảng (tt) Dữ liệu đồ thị quét dòng tuần hoàn – CV chip Pt dung dịch K3Fe(CN)6 mM, pH 4,0 x y x y x y x y x y x y 0.4901 3.87E-08 0.5084 3.68E-08 0.5267 3.59E-08 0.545 3.54E-08 0.5634 3.46E-08 0.5817 3.56E-08 0.4916 3.85E-08 0.5099 3.68E-08 0.5283 3.57E-08 0.5466 3.54E-08 0.5649 3.47E-08 0.5832 3.56E-08 0.4932 3.83E-08 0.5115 3.68E-08 0.5298 3.56E-08 0.5481 3.54E-08 0.5664 3.48E-08 0.5847 3.54E-08 0.4947 3.81E-08 0.513 3.68E-08 0.5313 3.54E-08 0.5496 3.53E-08 0.5679 3.49E-08 0.5862 3.51E-08 0.4962 3.79E-08 0.5145 3.68E-08 0.5328 3.53E-08 0.5511 3.52E-08 0.5695 3.50E-08 0.5878 3.54E-08 0.4977 3.77E-08 0.5161 3.68E-08 0.5344 3.53E-08 0.5527 3.51E-08 0.571 3.52E-08 0.5893 3.46E-08 0.4993 3.75E-08 0.5176 3.67E-08 0.5359 3.52E-08 0.5542 3.50E-08 0.5725 3.53E-08 0.5908 3.40E-08 0.5008 3.73E-08 0.5191 3.66E-08 0.5374 3.52E-08 0.5557 3.49E-08 0.574 3.55E-08 0.5923 3.40E-08 0.5023 3.71E-08 0.5206 3.65E-08 0.5389 3.52E-08 0.5573 3.48E-08 0.5756 3.56E-08 0.5939 3.41E-08 0.5038 3.70E-08 0.5222 3.64E-08 0.5405 3.53E-08 0.5588 3.47E-08 0.5771 3.57E-08 0.5954 3.41E-08 0.5054 3.69E-08 0.5237 3.62E-08 0.542 3.53E-08 0.5603 3.46E-08 0.5786 3.57E-08 0.5969 3.44E-08 0.5069 3.68E-08 0.5252 3.60E-08 0.5435 3.54E-08 0.5618 3.46E-08 0.5801 3.56E-08 0.5984 3.22E-08 Bảng Dữ liệu đồ thị quét dòng tuần hoàn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 với x: quét (V), y: cƣờng độ dòng (A) x -0.1 y -3.81E-07 -0.0618 -2.23E-07 x y -0.0237 -1.63E-07 0.0145 -1.22E-07 0.0526 -8.46E-08 0.0908 -4.39E-08 -0.0221 -1.61E-07 0.016 -1.21E-07 0.0542 -8.31E-08 0.0923 -4.22E-08 0.0176 -1.19E-07 0.0557 -8.15E-08 0.0938 -4.04E-08 0.0572 -8.00E-08 0.0954 -3.87E-08 0.0969 -3.69E-08 x y -0.0984 -4.82E-07 -0.0603 -2.20E-07 -0.0969 -3.52E-07 -0.0588 -2.17E-07 -0.0206 -1.59E-07 x -0.0954 -4.42E-07 -0.0572 -2.14E-07 -0.0191 -1.57E-07 0.0191 -1.18E-07 x -0.0938 -3.24E-07 -0.0557 -2.11E-07 -0.0176 -1.56E-07 0.0206 -1.16E-07 0.0588 -7.84E-08 x -0.0923 -3.94E-07 -0.0542 -2.08E-07 -0.016 -1.54E-07 0.0221 -1.15E-07 0.0603 -7.68E-08 0.0984 -3.52E-08 -0.0908 -3.17E-07 -0.0526 -2.05E-07 -0.0145 -1.52E-07 0.0237 -1.13E-07 0.0618 -7.52E-08 0.1 -3.34E-08 -0.0893 -3.54E-07 -0.0511 -2.02E-07 -0.013 -1.51E-07 0.0252 -1.12E-07 0.0633 -7.36E-08 0.1015 -3.16E-08 -0.0877 -3.08E-07 -0.0496 -2.00E-07 -0.0114 -1.49E-07 0.0267 -1.10E-07 0.0649 -7.20E-08 0.103 -2.98E-08 -0.0862 -3.09E-07 -0.0481 -1.97E-07 -0.0099 -1.47E-07 0.0282 -1.09E-07 0.0664 -7.04E-08 0.1045 -2.81E-08 -0.0847 -3.01E-07 -0.0465 -1.94E-07 -0.0084 -1.46E-07 0.0298 -1.07E-07 0.0679 -6.88E-08 0.106 -2.63E-08 -0.0832 -2.94E-07 -0.045 -1.92E-07 -0.0069 -1.44E-07 0.0313 -1.06E-07 0.0694 -6.71E-08 0.1076 -2.45E-08 -0.0816 -2.82E-07 -0.0435 -1.89E-07 -0.0053 -1.43E-07 0.0328 -1.04E-07 0.071 -6.55E-08 0.1091 -2.27E-08 -0.0801 -2.78E-07 -0.042 -1.87E-07 -0.0038 -1.41E-07 0.0343 -1.03E-07 0.0725 -6.39E-08 0.1106 -2.10E-08 -0.0786 -2.69E-07 -0.0404 -1.85E-07 -0.0023 -1.39E-07 0.0359 -1.01E-07 0.074 -6.22E-08 0.1122 -1.92E-08 -0.0771 -2.66E-07 -0.0389 -1.83E-07 -0.0008 -1.38E-07 0.0374 -9.99E-08 0.0755 -6.06E-08 0.1137 -1.74E-08 -0.0755 -2.58E-07 -0.0374 -1.80E-07 0.0008 -1.36E-07 0.0389 -9.84E-08 0.0771 -5.90E-08 0.1152 -1.56E-08 -0.074 -2.55E-07 -0.0359 -1.78E-07 0.0023 -1.35E-07 0.0404 -9.69E-08 0.0786 -5.73E-08 0.1167 -1.38E-08 -0.0725 -2.49E-07 -0.0343 -1.76E-07 0.0038 -1.33E-07 0.042 -9.53E-08 0.0801 -5.57E-08 0.1183 -1.21E-08 -0.071 y y y -2.46E-07 -0.0328 -1.74E-07 0.0053 -1.32E-07 0.0435 -9.38E-08 0.0816 -5.40E-08 0.1198 -1.03E-08 -0.0694 -2.41E-07 -0.0313 -1.72E-07 0.0069 -1.30E-07 0.045 -9.23E-08 0.0832 -5.23E-08 0.1213 -8.48E-09 -0.0679 -2.37E-07 -0.0298 -1.70E-07 0.0084 -1.29E-07 0.0465 -9.08E-08 0.0847 -5.07E-08 0.1228 -6.69E-09 -0.0664 -2.33E-07 -0.0282 -1.68E-07 0.0099 -1.27E-07 0.0481 -8.93E-08 0.0862 -4.90E-08 0.1244 -4.89E-09 -0.0649 -2.30E-07 -0.0267 -1.66E-07 0.0114 -1.26E-07 0.0496 -8.77E-08 0.0877 -4.73E-08 0.1259 -3.09E-09 -0.0633 -2.26E-07 -0.0252 -1.64E-07 0.013 -1.24E-07 0.0511 -8.62E-08 0.0893 -4.56E-08 0.1274 -1.28E-09 0.1289 5.26E-10 0.1305 2.34E-09 0.132 4.14E-09 0.1335 5.95E-09 0.135 7.75E-09 0.1366 9.55E-09 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 x y 0.2022 0.1381 1.14E-08 x 0.1396 1.31E-08 0.2037 7.79E-08 y 7.92E-08 0.2663 1.19E-07 0.3304 1.44E-07 0.3944 1.65E-07 0.4585 1.86E-07 0.2678 1.20E-07 0.3319 1.44E-07 0.396 1.65E-07 0.4601 1.86E-07 0.3334 1.45E-07 0.3975 1.66E-07 0.4616 1.87E-07 0.399 1.66E-07 0.4631 1.87E-07 0.4646 1.88E-07 x y 0.1411 1.49E-08 0.2052 8.05E-08 0.2693 1.20E-07 x y 0.1427 1.67E-08 0.2068 8.17E-08 0.2708 1.21E-07 0.3349 1.46E-07 x 0.1442 1.84E-08 0.2083 8.30E-08 0.2724 1.22E-07 0.3365 1.46E-07 0.4005 1.67E-07 x 0.1457 2.02E-08 0.2098 8.42E-08 0.2739 1.22E-07 0.338 1.47E-07 0.4021 1.67E-07 0.4662 1.88E-07 0.1472 2.20E-08 0.2113 8.54E-08 0.2754 1.23E-07 0.3395 1.47E-07 0.4036 1.68E-07 0.4677 1.89E-07 0.1488 2.37E-08 0.2129 8.65E-08 0.2769 1.24E-07 0.341 1.48E-07 0.4051 1.68E-07 0.4692 1.89E-07 0.1503 2.54E-08 0.2144 8.77E-08 0.2785 1.24E-07 0.3426 1.48E-07 0.4066 1.69E-07 0.4707 1.90E-07 0.1518 2.72E-08 0.2159 8.88E-08 0.28 1.25E-07 0.3441 1.49E-07 0.4082 1.69E-07 0.4723 1.90E-07 0.1534 2.89E-08 0.2174 8.99E-08 0.2815 1.26E-07 0.3456 1.49E-07 0.4097 1.69E-07 0.4738 1.91E-07 0.1549 3.06E-08 0.219 9.10E-08 0.2831 1.26E-07 0.3471 1.50E-07 0.4112 1.70E-07 0.4753 1.91E-07 0.1564 3.23E-08 0.2205 9.21E-08 0.2846 1.27E-07 0.3487 1.50E-07 0.4128 1.70E-07 0.4768 1.92E-07 0.1579 3.40E-08 0.222 9.32E-08 0.2861 1.28E-07 0.3502 1.51E-07 0.4143 1.71E-07 0.4784 1.92E-07 0.1595 3.57E-08 0.2235 9.42E-08 0.2876 1.28E-07 0.3517 1.51E-07 0.4158 1.71E-07 0.4799 1.93E-07 0.161 3.74E-08 0.2251 9.53E-08 0.2892 1.29E-07 0.3532 1.52E-07 0.4173 1.72E-07 0.4814 1.93E-07 0.1625 3.91E-08 0.2266 9.63E-08 0.2907 1.29E-07 0.3548 1.52E-07 0.4189 1.72E-07 0.4829 1.94E-07 0.164 4.07E-08 0.2281 9.73E-08 0.2922 1.30E-07 0.3563 1.53E-07 0.4204 1.73E-07 0.4845 1.94E-07 0.1656 4.24E-08 0.2296 9.83E-08 0.2937 1.31E-07 0.3578 1.53E-07 0.4219 1.73E-07 0.486 1.95E-07 0.1671 4.40E-08 0.2312 9.93E-08 0.2953 1.31E-07 0.3593 1.54E-07 0.4234 1.74E-07 0.4875 1.95E-07 0.1686 4.57E-08 0.2327 1.00E-07 0.2968 1.32E-07 0.3609 1.54E-07 0.425 1.75E-07 0.489 1.96E-07 0.1701 4.73E-08 0.2342 1.01E-07 0.2983 1.32E-07 0.3624 1.55E-07 0.4265 1.75E-07 0.4906 1.96E-07 0.1717 4.89E-08 0.2357 1.02E-07 0.2998 1.33E-07 0.3639 1.55E-07 0.428 1.76E-07 0.4921 1.97E-07 0.1732 5.05E-08 0.2373 1.03E-07 0.3014 1.34E-07 0.3654 1.56E-07 0.4295 1.76E-07 0.4936 1.97E-07 0.1747 5.20E-08 0.2388 1.04E-07 0.3029 1.34E-07 0.367 1.56E-07 0.4311 1.77E-07 0.4951 1.98E-07 0.1762 5.36E-08 0.2403 1.05E-07 0.3044 1.35E-07 0.3685 1.57E-07 0.4326 1.77E-07 0.4967 1.98E-07 0.1778 5.51E-08 0.2419 1.06E-07 0.3059 1.35E-07 0.37 1.57E-07 0.4341 1.78E-07 0.4982 1.99E-07 0.1793 5.66E-08 0.2434 1.07E-07 0.3075 1.36E-07 0.3716 1.58E-07 0.4356 1.78E-07 0.4997 1.99E-07 0.1808 5.81E-08 0.2449 1.08E-07 0.309 1.36E-07 0.3731 1.58E-07 0.4372 1.79E-07 0.5013 2.00E-07 0.1823 5.96E-08 0.2464 1.08E-07 0.3105 1.37E-07 0.3746 1.58E-07 0.4387 1.79E-07 0.5028 2.00E-07 0.1839 6.11E-08 0.248 1.09E-07 0.312 1.38E-07 0.3761 1.59E-07 0.4402 1.80E-07 0.5043 2.01E-07 0.1854 6.25E-08 0.2495 1.10E-07 0.3136 1.38E-07 0.3777 1.59E-07 0.4417 1.80E-07 0.5058 2.01E-07 0.1869 6.40E-08 0.251 1.11E-07 0.3151 1.39E-07 0.3792 1.60E-07 0.4433 1.81E-07 0.5074 2.02E-07 0.1884 6.55E-08 0.2525 1.12E-07 0.3166 1.39E-07 0.3807 1.60E-07 0.4448 1.81E-07 0.5089 2.02E-07 0.19 6.69E-08 0.2541 1.13E-07 0.3181 1.40E-07 0.3822 1.61E-07 0.4463 1.82E-07 0.5104 2.03E-07 0.1915 6.83E-08 0.2556 1.13E-07 0.3197 1.40E-07 0.3838 1.61E-07 0.4478 1.82E-07 0.5119 2.03E-07 0.193 6.97E-08 0.2571 1.14E-07 0.3212 1.41E-07 0.3853 1.62E-07 0.4494 1.83E-07 0.5135 2.04E-07 0.1945 7.11E-08 0.2586 1.15E-07 0.3227 1.41E-07 0.3868 1.62E-07 0.4509 1.83E-07 0.515 2.04E-07 0.1961 7.25E-08 0.2602 1.16E-07 0.3242 1.42E-07 0.3883 1.63E-07 0.4524 1.84E-07 0.5165 2.05E-07 0.1976 7.39E-08 0.2617 1.16E-07 0.3258 1.42E-07 0.3899 1.63E-07 0.4539 1.84E-07 0.518 2.05E-07 0.1991 7.53E-08 0.2632 1.17E-07 0.3273 1.43E-07 0.3914 1.64E-07 0.4555 1.85E-07 0.5196 2.06E-07 0.2007 7.66E-08 0.2647 1.18E-07 0.3288 1.43E-07 0.3929 1.64E-07 0.457 1.85E-07 0.5211 2.06E-07 y y 0.5226 2.07E-07 0.5241 2.07E-07 0.5257 2.08E-07 0.5272 2.08E-07 0.5287 2.09E-07 0.5302 2.09E-07 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 x y 0.5959 0.5318 2.10E-07 x 0.5333 2.10E-07 0.5974 2.28E-07 y 2.29E-07 0.6599 2.45E-07 0.724 2.64E-07 0.7881 2.93E-07 0.8522 3.36E-07 0.6615 2.45E-07 0.7256 2.65E-07 0.7896 2.94E-07 0.8537 3.37E-07 0.7271 2.66E-07 0.7912 2.94E-07 0.8553 3.39E-07 0.7927 2.95E-07 0.8568 3.40E-07 0.8583 3.41E-07 x y 0.5348 2.11E-07 0.5989 2.29E-07 0.663 2.46E-07 x y 0.5363 2.11E-07 0.6004 2.30E-07 0.6645 2.46E-07 0.7286 2.66E-07 x 0.5379 2.12E-07 0.602 2.30E-07 0.666 2.47E-07 0.7301 2.67E-07 0.7942 2.96E-07 x 0.5394 2.12E-07 0.6035 2.30E-07 0.6676 2.47E-07 0.7317 2.67E-07 0.7957 2.97E-07 0.8598 3.43E-07 0.5409 2.13E-07 0.605 2.31E-07 0.6691 2.47E-07 0.7332 2.68E-07 0.7973 2.98E-07 0.8614 3.44E-07 0.5425 2.13E-07 0.6065 2.31E-07 0.6706 2.48E-07 0.7347 2.68E-07 0.7988 2.99E-07 0.8629 3.45E-07 0.544 2.14E-07 0.6081 2.32E-07 0.6721 2.48E-07 0.7362 2.69E-07 0.8003 2.99E-07 0.8644 3.47E-07 0.5455 2.14E-07 0.6096 2.32E-07 0.6737 2.49E-07 0.7378 2.69E-07 0.8018 3.00E-07 0.8659 3.48E-07 0.547 2.15E-07 0.6111 2.32E-07 0.6752 2.49E-07 0.7393 2.70E-07 0.8034 3.01E-07 0.8675 3.49E-07 0.5486 2.15E-07 0.6126 2.33E-07 0.6767 2.50E-07 0.7408 2.71E-07 0.8049 3.02E-07 0.869 3.51E-07 0.5501 2.15E-07 0.6142 2.33E-07 0.6783 2.50E-07 0.7423 2.71E-07 0.8064 3.03E-07 0.8705 3.52E-07 0.5516 2.16E-07 0.6157 2.34E-07 0.6798 2.50E-07 0.7439 2.72E-07 0.808 3.04E-07 0.872 3.54E-07 0.5531 2.16E-07 0.6172 2.34E-07 0.6813 2.51E-07 0.7454 2.72E-07 0.8095 3.05E-07 0.8736 3.55E-07 0.5547 2.17E-07 0.6187 2.34E-07 0.6828 2.51E-07 0.7469 2.73E-07 0.811 3.06E-07 0.8751 3.57E-07 0.5562 2.17E-07 0.6203 2.35E-07 0.6844 2.52E-07 0.7484 2.74E-07 0.8125 3.07E-07 0.8766 3.58E-07 0.5577 2.18E-07 0.6218 2.35E-07 0.6859 2.52E-07 0.75 2.74E-07 0.8141 3.08E-07 0.8781 3.60E-07 0.5592 2.18E-07 0.6233 2.35E-07 0.6874 2.53E-07 0.7515 2.75E-07 0.8156 3.09E-07 0.8797 3.61E-07 0.5608 2.19E-07 0.6248 2.36E-07 0.6889 2.53E-07 0.753 2.76E-07 0.8171 3.10E-07 0.8812 3.63E-07 0.5623 2.19E-07 0.6264 2.36E-07 0.6905 2.54E-07 0.7545 2.76E-07 0.8186 3.11E-07 0.8827 3.65E-07 0.5638 2.19E-07 0.6279 2.37E-07 0.692 2.54E-07 0.7561 2.77E-07 0.8202 3.12E-07 0.8842 3.66E-07 0.5653 2.20E-07 0.6294 2.37E-07 0.6935 2.55E-07 0.7576 2.78E-07 0.8217 3.13E-07 0.8858 3.68E-07 0.5669 2.20E-07 0.631 2.37E-07 0.695 2.55E-07 0.7591 2.78E-07 0.8232 3.14E-07 0.8873 3.69E-07 0.5684 2.21E-07 0.6325 2.38E-07 0.6966 2.55E-07 0.7607 2.79E-07 0.8247 3.15E-07 0.8888 3.71E-07 0.5699 2.21E-07 0.634 2.38E-07 0.6981 2.56E-07 0.7622 2.80E-07 0.8263 3.16E-07 0.8904 3.73E-07 0.5714 2.21E-07 0.6355 2.38E-07 0.6996 2.56E-07 0.7637 2.80E-07 0.8278 3.17E-07 0.8919 3.74E-07 0.573 2.22E-07 0.6371 2.39E-07 0.7011 2.57E-07 0.7652 2.81E-07 0.8293 3.18E-07 0.8934 3.76E-07 0.5745 2.22E-07 0.6386 2.39E-07 0.7027 2.57E-07 0.7668 2.82E-07 0.8308 3.19E-07 0.8949 3.78E-07 0.576 2.23E-07 0.6401 2.40E-07 0.7042 2.58E-07 0.7683 2.82E-07 0.8324 3.20E-07 0.8965 3.79E-07 0.5775 2.23E-07 0.6416 2.40E-07 0.7057 2.58E-07 0.7698 2.83E-07 0.8339 3.21E-07 0.898 3.81E-07 0.5791 2.24E-07 0.6432 2.40E-07 0.7072 2.59E-07 0.7713 2.84E-07 0.8354 3.22E-07 0.8995 3.83E-07 0.5806 2.24E-07 0.6447 2.41E-07 0.7088 2.59E-07 0.7729 2.85E-07 0.8369 3.24E-07 0.901 3.85E-07 0.5821 2.24E-07 0.6462 2.41E-07 0.7103 2.60E-07 0.7744 2.85E-07 0.8385 3.25E-07 0.9026 3.86E-07 0.5836 2.25E-07 0.6477 2.42E-07 0.7118 2.60E-07 0.7759 2.86E-07 0.84 3.26E-07 0.9041 3.88E-07 0.5852 2.25E-07 0.6493 2.42E-07 0.7133 2.61E-07 0.7774 2.87E-07 0.8415 3.27E-07 0.9056 3.90E-07 0.5867 2.26E-07 0.6508 2.43E-07 0.7149 2.61E-07 0.779 2.88E-07 0.843 3.28E-07 0.9071 3.92E-07 0.5882 2.26E-07 0.6523 2.43E-07 0.7164 2.62E-07 0.7805 2.89E-07 0.8446 3.30E-07 0.9087 3.94E-07 0.5898 2.27E-07 0.6538 2.43E-07 0.7179 2.62E-07 0.782 2.89E-07 0.8461 3.31E-07 0.9102 3.96E-07 0.5913 2.27E-07 0.6554 2.44E-07 0.7195 2.63E-07 0.7835 2.90E-07 0.8476 3.32E-07 0.9117 3.98E-07 0.5928 2.28E-07 0.6569 2.44E-07 0.721 2.63E-07 0.7851 2.91E-07 0.8492 3.33E-07 0.9132 4.00E-07 0.5943 2.28E-07 0.6584 2.45E-07 0.7225 2.64E-07 0.7866 2.92E-07 0.8507 3.35E-07 0.9148 4.02E-07 y y 0.9163 4.04E-07 0.9178 4.06E-07 0.9193 4.08E-07 0.9209 4.10E-07 0.9224 4.13E-07 0.9239 4.15E-07 Bảng (tt) Dữ liệu đồ thị qt dòng tuần hồn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 x y 0.9895 0.9254 4.17E-07 x 0.927 4.19E-07 0.9911 5.38E-07 y 5.39E-07 0.9468 2.92E-07 0.8827 1.65E-07 0.8186 8.73E-08 0.7545 3.55E-08 0.9453 2.88E-07 0.8812 1.63E-07 0.8171 8.59E-08 0.753 3.45E-08 0.8797 1.61E-07 0.8156 8.44E-08 0.7515 3.36E-08 0.8141 8.29E-08 0.75 3.26E-08 0.7484 3.16E-08 x y 0.9285 4.22E-07 0.9926 5.38E-07 0.9438 2.84E-07 x y 0.93 4.24E-07 0.9941 5.37E-07 0.9422 2.80E-07 0.8781 1.59E-07 x 0.9315 4.26E-07 0.9956 5.35E-07 0.9407 2.77E-07 0.8766 1.56E-07 0.8125 8.15E-08 x 0.9331 4.29E-07 0.9972 5.32E-07 0.9392 2.73E-07 0.8751 1.54E-07 0.811 8.01E-08 0.7469 3.06E-08 0.9346 4.31E-07 0.9987 5.29E-07 0.9377 2.69E-07 0.8736 1.52E-07 0.8095 7.86E-08 0.7454 2.97E-08 0.9361 4.33E-07 5.24E-07 0.9361 2.66E-07 0.872 1.50E-07 0.808 7.72E-08 0.7439 2.87E-08 0.9377 4.36E-07 0.9987 5.19E-07 0.9346 2.62E-07 0.8705 1.48E-07 0.8064 7.58E-08 0.7423 2.77E-08 0.9392 4.38E-07 0.9972 5.13E-07 0.9331 2.58E-07 0.869 1.46E-07 0.8049 7.44E-08 0.7408 2.68E-08 0.9407 4.41E-07 0.9956 5.07E-07 0.9315 2.55E-07 0.8675 1.44E-07 0.8034 7.30E-08 0.7393 2.58E-08 0.9422 4.43E-07 0.9941 5.00E-07 0.93 2.51E-07 0.8659 1.41E-07 0.8018 7.17E-08 0.7378 2.49E-08 0.9438 4.46E-07 0.9926 4.92E-07 0.9285 2.48E-07 0.8644 1.39E-07 0.8003 7.03E-08 0.7362 2.40E-08 0.9453 4.48E-07 0.9911 4.84E-07 0.927 2.45E-07 0.8629 1.37E-07 0.7988 6.90E-08 0.7347 2.30E-08 0.9468 4.51E-07 0.9895 4.76E-07 0.9254 2.41E-07 0.8614 1.35E-07 0.7973 6.77E-08 0.7332 2.21E-08 0.9483 4.53E-07 0.988 4.67E-07 0.9239 2.38E-07 0.8598 1.34E-07 0.7957 6.64E-08 0.7317 2.12E-08 0.9499 4.56E-07 0.9865 4.58E-07 0.9224 2.35E-07 0.8583 1.32E-07 0.7942 6.51E-08 0.7301 2.03E-08 0.9514 4.58E-07 0.985 4.49E-07 0.9209 2.32E-07 0.8568 1.30E-07 0.7927 6.38E-08 0.7286 1.95E-08 0.9529 4.61E-07 0.9834 4.40E-07 0.9193 2.29E-07 0.8553 1.28E-07 0.7912 6.26E-08 0.7271 1.86E-08 0.9544 4.64E-07 0.9819 4.31E-07 0.9178 2.26E-07 0.8537 1.26E-07 0.7896 6.14E-08 0.7256 1.78E-08 0.956 4.67E-07 0.9804 4.22E-07 0.9163 2.23E-07 0.8522 1.24E-07 0.7881 6.01E-08 0.724 1.69E-08 0.9575 4.70E-07 0.9789 4.13E-07 0.9148 2.20E-07 0.8507 1.22E-07 0.7866 5.89E-08 0.7225 1.61E-08 0.959 4.73E-07 0.9773 4.05E-07 0.9132 2.17E-07 0.8492 1.20E-07 0.7851 5.77E-08 0.721 1.53E-08 0.9605 4.76E-07 0.9758 3.96E-07 0.9117 2.14E-07 0.8476 1.19E-07 0.7835 5.65E-08 0.7195 1.44E-08 0.9621 4.79E-07 0.9743 3.88E-07 0.9102 2.11E-07 0.8461 1.17E-07 0.782 5.53E-08 0.7179 1.36E-08 0.9636 4.82E-07 0.9727 3.81E-07 0.9087 2.08E-07 0.8446 1.15E-07 0.7805 5.41E-08 0.7164 1.28E-08 0.9651 4.86E-07 0.9712 3.74E-07 0.9071 2.06E-07 0.843 1.13E-07 0.779 5.29E-08 0.7149 1.20E-08 0.9666 4.89E-07 0.9697 3.67E-07 0.9056 2.03E-07 0.8415 1.12E-07 0.7774 5.18E-08 0.7133 1.12E-08 0.9682 4.93E-07 0.9682 3.60E-07 0.9041 2.00E-07 0.84 1.10E-07 0.7759 5.06E-08 0.7118 1.04E-08 0.9697 4.97E-07 0.9666 3.54E-07 0.9026 1.97E-07 0.8385 1.08E-07 0.7744 4.94E-08 0.7103 9.53E-09 0.9712 5.01E-07 0.9651 3.48E-07 0.901 1.95E-07 0.8369 1.06E-07 0.7729 4.83E-08 0.7088 8.72E-09 0.9727 5.05E-07 0.9636 3.42E-07 0.8995 1.92E-07 0.8354 1.05E-07 0.7713 4.71E-08 0.7072 7.92E-09 0.9743 5.09E-07 0.9621 3.36E-07 0.898 1.89E-07 0.8339 1.03E-07 0.7698 4.60E-08 0.7057 7.12E-09 0.9758 5.13E-07 0.9605 3.31E-07 0.8965 1.87E-07 0.8324 1.01E-07 0.7683 4.49E-08 0.7042 6.33E-09 0.9773 5.17E-07 0.959 3.26E-07 0.8949 1.84E-07 0.8308 9.97E-08 0.7668 4.38E-08 0.7027 5.55E-09 0.9789 5.21E-07 0.9575 3.22E-07 0.8934 1.82E-07 0.8293 9.81E-08 0.7652 4.27E-08 0.7011 4.79E-09 0.9804 5.25E-07 0.956 3.17E-07 0.8919 1.79E-07 0.8278 9.65E-08 0.7637 4.17E-08 0.6996 4.04E-09 0.9819 5.28E-07 0.9544 3.13E-07 0.8904 1.77E-07 0.8263 9.49E-08 0.7622 4.06E-08 0.6981 3.31E-09 0.9834 5.31E-07 0.9529 3.08E-07 0.8888 1.75E-07 0.8247 9.34E-08 0.7607 3.96E-08 0.6966 2.59E-09 0.985 5.34E-07 0.9514 3.04E-07 0.8873 1.72E-07 0.8232 9.18E-08 0.7591 3.85E-08 0.695 1.89E-09 0.9865 5.36E-07 0.9499 3.00E-07 0.8858 1.70E-07 0.8217 9.03E-08 0.7576 3.75E-08 0.6935 1.20E-09 0.988 5.37E-07 0.9483 2.96E-07 0.8842 1.68E-07 0.8202 8.88E-08 0.7561 3.65E-08 0.692 5.21E-10 y y 0.6905 -1.43E-10 0.6889 -7.97E-10 0.6874 -1.44E-09 0.6859 -2.08E-09 0.6844 -2.72E-09 0.6828 -3.36E-09 Bảng (tt) Dữ liệu đồ thị quét dòng tuần hoàn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 x y 0.6172 0.6813 -4.00E-09 x 0.6798 -4.63E-09 0.6157 -2.78E-08 y -2.82E-08 0.5531 -4.60E-08 0.489 -6.10E-08 0.425 -7.55E-08 0.3609 -9.07E-08 0.5516 -4.64E-08 0.4875 -6.13E-08 0.4234 -7.59E-08 0.3593 -9.11E-08 0.486 -6.17E-08 0.4219 -7.62E-08 0.3578 -9.15E-08 0.4204 -7.65E-08 0.3563 -9.19E-08 0.3548 -9.23E-08 x y 0.6783 -5.27E-09 0.6142 -2.87E-08 0.5501 -4.68E-08 x y 0.6767 -5.91E-09 0.6126 -2.92E-08 0.5486 -4.71E-08 0.4845 -6.20E-08 x 0.6752 -6.55E-09 0.6111 -2.97E-08 0.547 -4.75E-08 0.4829 -6.23E-08 0.4189 -7.69E-08 x 0.6737 -7.19E-09 0.6096 -3.02E-08 0.5455 -4.79E-08 0.4814 -6.27E-08 0.4173 -7.72E-08 0.3532 -9.27E-08 0.6721 -7.83E-09 0.6081 -3.07E-08 0.544 -4.83E-08 0.4799 -6.30E-08 0.4158 -7.75E-08 0.3517 -9.30E-08 0.6706 -8.47E-09 0.6065 -3.12E-08 0.5425 -4.87E-08 0.4784 -6.34E-08 0.4143 -7.79E-08 0.3502 -9.34E-08 0.6691 -9.10E-09 0.605 -3.16E-08 0.5409 -4.91E-08 0.4768 -6.38E-08 0.4128 -7.82E-08 0.3487 -9.38E-08 0.6676 -9.73E-09 0.6035 -3.21E-08 0.5394 -4.95E-08 0.4753 -6.41E-08 0.4112 -7.86E-08 0.3471 -9.42E-08 0.666 -1.04E-08 0.602 -3.26E-08 0.5379 -4.99E-08 0.4738 -6.45E-08 0.4097 -7.90E-08 0.3456 -9.46E-08 0.6645 -1.10E-08 0.6004 -3.31E-08 0.5363 -5.03E-08 0.4723 -6.49E-08 0.4082 -7.93E-08 0.3441 -9.51E-08 0.663 -1.16E-08 0.5989 -3.36E-08 0.5348 -5.07E-08 0.4707 -6.53E-08 0.4066 -7.97E-08 0.3426 -9.55E-08 0.6615 -1.22E-08 0.5974 -3.40E-08 0.5333 -5.11E-08 0.4692 -6.56E-08 0.4051 -8.01E-08 0.341 -9.59E-08 0.6599 -1.28E-08 0.5959 -3.45E-08 0.5318 -5.15E-08 0.4677 -6.60E-08 0.4036 -8.04E-08 0.3395 -9.63E-08 0.6584 -1.33E-08 0.5943 -3.49E-08 0.5302 -5.18E-08 0.4662 -6.64E-08 0.4021 -8.08E-08 0.338 -9.67E-08 0.6569 -1.39E-08 0.5928 -3.53E-08 0.5287 -5.22E-08 0.4646 -6.67E-08 0.4005 -8.12E-08 0.3365 -9.72E-08 0.6554 -1.45E-08 0.5913 -3.58E-08 0.5272 -5.26E-08 0.4631 -6.71E-08 0.399 -8.16E-08 0.3349 -9.76E-08 0.6538 -1.51E-08 0.5898 -3.62E-08 0.5257 -5.29E-08 0.4616 -6.74E-08 0.3975 -8.19E-08 0.3334 -9.80E-08 0.6523 -1.56E-08 0.5882 -3.66E-08 0.5241 -5.33E-08 0.4601 -6.77E-08 0.396 -8.23E-08 0.3319 -9.85E-08 0.6508 -1.62E-08 0.5867 -3.70E-08 0.5226 -5.36E-08 0.4585 -6.80E-08 0.3944 -8.27E-08 0.3304 -9.89E-08 0.6493 -1.68E-08 0.5852 -3.74E-08 0.5211 -5.40E-08 0.457 -6.84E-08 0.3929 -8.30E-08 0.3288 -9.93E-08 0.6477 -1.73E-08 0.5836 -3.78E-08 0.5196 -5.43E-08 0.4555 -6.87E-08 0.3914 -8.34E-08 0.3273 -9.97E-08 0.6462 -1.79E-08 0.5821 -3.82E-08 0.518 -5.47E-08 0.4539 -6.90E-08 0.3899 -8.37E-08 0.3258 -1.00E-07 0.6447 -1.85E-08 0.5806 -3.86E-08 0.5165 -5.50E-08 0.4524 -6.93E-08 0.3883 -8.40E-08 0.3242 -1.01E-07 0.6432 -1.91E-08 0.5791 -3.91E-08 0.515 -5.53E-08 0.4509 -6.96E-08 0.3868 -8.44E-08 0.3227 -1.01E-07 0.6416 -1.96E-08 0.5775 -3.95E-08 0.5135 -5.57E-08 0.4494 -6.99E-08 0.3853 -8.47E-08 0.3212 -1.02E-07 0.6401 -2.02E-08 0.576 -3.99E-08 0.5119 -5.60E-08 0.4478 -7.03E-08 0.3838 -8.51E-08 0.3197 -1.02E-07 0.6386 -2.08E-08 0.5745 -4.04E-08 0.5104 -5.63E-08 0.4463 -7.06E-08 0.3822 -8.54E-08 0.3181 -1.02E-07 0.6371 -2.13E-08 0.573 -4.08E-08 0.5089 -5.67E-08 0.4448 -7.09E-08 0.3807 -8.58E-08 0.3166 -1.03E-07 0.6355 -2.19E-08 0.5714 -4.12E-08 0.5074 -5.70E-08 0.4433 -7.13E-08 0.3792 -8.61E-08 0.3151 -1.03E-07 0.634 -2.24E-08 0.5699 -4.17E-08 0.5058 -5.74E-08 0.4417 -7.16E-08 0.3777 -8.65E-08 0.3136 -1.04E-07 0.6325 -2.29E-08 0.5684 -4.21E-08 0.5043 -5.77E-08 0.4402 -7.20E-08 0.3761 -8.69E-08 0.312 -1.04E-07 0.631 -2.35E-08 0.5669 -4.25E-08 0.5028 -5.81E-08 0.4387 -7.24E-08 0.3746 -8.73E-08 0.3105 -1.05E-07 0.6294 -2.40E-08 0.5653 -4.29E-08 0.5013 -5.84E-08 0.4372 -7.27E-08 0.3731 -8.76E-08 0.309 -1.05E-07 0.6279 -2.45E-08 0.5638 -4.34E-08 0.4997 -5.87E-08 0.4356 -7.31E-08 0.3716 -8.80E-08 0.3075 -1.06E-07 0.6264 -2.50E-08 0.5623 -4.38E-08 0.4982 -5.91E-08 0.4341 -7.35E-08 0.37 -8.84E-08 0.3059 -1.06E-07 0.6248 -2.54E-08 0.5608 -4.41E-08 0.4967 -5.94E-08 0.4326 -7.38E-08 0.3685 -8.88E-08 0.3044 -1.07E-07 0.6233 -2.59E-08 0.5592 -4.45E-08 0.4951 -5.97E-08 0.4311 -7.42E-08 0.367 -8.92E-08 0.3029 -1.07E-07 0.6218 -2.64E-08 0.5577 -4.49E-08 0.4936 -6.00E-08 0.4295 -7.45E-08 0.3654 -8.96E-08 0.3014 -1.08E-07 0.6203 -2.68E-08 0.5562 -4.53E-08 0.4921 -6.04E-08 0.428 -7.49E-08 0.3639 -9.00E-08 0.2998 -1.09E-07 0.6187 -2.73E-08 0.5547 -4.57E-08 0.4906 -6.07E-08 0.4265 -7.52E-08 0.3624 -9.04E-08 0.2983 -1.09E-07 y y 0.2968 -1.10E-07 0.2953 -1.10E-07 0.2937 -1.11E-07 0.2922 -1.11E-07 0.2907 -1.12E-07 0.2892 -1.12E-07 Bảng (tt) Dữ liệu đồ thị quét dòng tuần hoàn – CV chip Pt/CA/GAD/COx dung dịch PBS, pH 7,0 x y 0.2235 0.2876 -1.13E-07 x 0.2861 -1.13E-07 0.222 -1.46E-07 y -1.47E-07 0.1595 -2.27E-07 0.0954 -4.04E-07 0.0313 -5.23E-07 -0.0328 -4.98E-07 0.1579 -2.30E-07 0.0938 -4.09E-07 0.0298 -5.23E-07 -0.0343 -4.97E-07 0.0923 -4.13E-07 0.0282 -5.23E-07 -0.0359 -4.96E-07 0.0267 -5.24E-07 -0.0374 -4.95E-07 x y 0.2846 -1.14E-07 0.2205 -1.48E-07 0.1564 -2.33E-07 x y 0.2831 -1.14E-07 0.219 -1.50E-07 0.1549 -2.36E-07 0.0908 -4.18E-07 x 0.2815 -1.15E-07 0.2174 -1.51E-07 0.1534 -2.39E-07 0.0893 -4.23E-07 0.0252 -5.24E-07 0.28 -1.16E-07 0.2159 -1.52E-07 0.1518 -2.42E-07 0.0877 -4.27E-07 0.0237 -5.24E-07 -0.0404 -4.93E-07 0.2785 -1.16E-07 0.2144 -1.53E-07 0.1503 -2.46E-07 0.0862 -4.32E-07 0.0221 -5.24E-07 -0.042 0.2769 -1.17E-07 0.2129 -1.55E-07 0.1488 -2.49E-07 0.0847 -4.36E-07 0.0206 -5.24E-07 -0.0435 -4.91E-07 0.2754 -1.18E-07 0.2113 -1.56E-07 0.1472 -2.53E-07 0.0832 -4.41E-07 0.0191 -5.24E-07 -0.045 0.2739 -1.18E-07 0.2098 -1.57E-07 0.1457 -2.56E-07 0.0816 -4.45E-07 0.0176 -5.23E-07 -0.0465 -4.89E-07 0.2724 -1.19E-07 0.2083 -1.59E-07 0.1442 -2.60E-07 0.0801 -4.49E-07 0.016 -5.23E-07 -0.0481 -4.88E-07 0.2708 -1.20E-07 0.2068 -1.60E-07 0.1427 -2.64E-07 0.0786 -4.53E-07 0.0145 -5.23E-07 -0.0496 -4.87E-07 0.2693 -1.20E-07 0.2052 -1.62E-07 0.1411 -2.67E-07 0.0771 -4.57E-07 0.013 -5.23E-07 -0.0511 -4.86E-07 0.2678 -1.21E-07 0.2037 -1.63E-07 0.1396 -2.71E-07 0.0755 -4.61E-07 0.0114 -5.22E-07 -0.0526 -4.85E-07 0.2663 -1.22E-07 0.2022 -1.65E-07 0.1381 -2.75E-07 0.074 -4.65E-07 0.0099 -5.22E-07 -0.0542 -4.85E-07 0.2647 -1.22E-07 0.2007 -1.66E-07 0.1366 -2.79E-07 0.0725 -4.69E-07 0.0084 -5.21E-07 -0.0557 -4.84E-07 0.2632 -1.23E-07 0.1991 -1.68E-07 0.135 -2.83E-07 0.071 -4.72E-07 0.0069 -5.21E-07 -0.0572 -4.83E-07 0.2617 -1.24E-07 0.1976 -1.70E-07 0.1335 -2.87E-07 0.0694 -4.76E-07 0.0053 -5.20E-07 -0.0588 -4.82E-07 0.2602 -1.24E-07 0.1961 -1.71E-07 0.132 -2.91E-07 0.0679 -4.79E-07 0.0038 -5.19E-07 -0.0603 -4.81E-07 0.2586 -1.25E-07 0.1945 -1.73E-07 0.1305 -2.96E-07 0.0664 -4.83E-07 0.0023 -5.19E-07 -0.0618 -4.80E-07 0.2571 -1.26E-07 0.193 -1.75E-07 0.1289 -3.00E-07 0.0649 -4.86E-07 0.0008 -5.18E-07 -0.0633 -4.79E-07 0.2556 -1.27E-07 0.1915 -1.77E-07 0.1274 -3.04E-07 0.0633 -4.89E-07 -0.0008 -5.17E-07 -0.0649 -4.77E-07 0.2541 -1.27E-07 0.19 -1.79E-07 0.1259 -3.09E-07 0.0618 -4.91E-07 -0.0023 -5.17E-07 -0.0664 -4.76E-07 0.2525 -1.28E-07 0.1884 -1.80E-07 0.1244 -3.13E-07 0.0603 -4.94E-07 -0.0038 -5.16E-07 -0.0679 -4.75E-07 0.251 -1.29E-07 0.1869 -1.82E-07 0.1228 -3.18E-07 0.0588 -4.97E-07 -0.0053 -5.15E-07 -0.0694 -4.74E-07 0.2495 -1.30E-07 0.1854 -1.84E-07 0.1213 -3.22E-07 0.0572 -4.99E-07 -0.0069 -5.14E-07 -0.071 0.248 -1.31E-07 0.1839 -1.86E-07 0.1198 -3.27E-07 0.0557 -5.02E-07 -0.0084 -5.13E-07 -0.0725 -4.73E-07 0.2464 -1.31E-07 0.1823 -1.88E-07 0.1183 -3.31E-07 0.0542 -5.04E-07 -0.0099 -5.12E-07 -0.074 0.2449 -1.32E-07 0.1808 -1.91E-07 0.1167 -3.36E-07 0.0526 -5.06E-07 -0.0114 -5.11E-07 -0.0755 -4.70E-07 0.2434 -1.33E-07 0.1793 -1.93E-07 0.1152 -3.41E-07 0.0511 -5.08E-07 -0.013 -5.11E-07 -0.0771 -4.66E-07 0.2419 -1.34E-07 0.1778 -1.95E-07 0.1137 -3.46E-07 0.0496 -5.10E-07 -0.0145 -5.10E-07 -0.0786 -4.69E-07 0.2403 -1.35E-07 0.1762 -1.97E-07 0.1122 -3.50E-07 0.0481 -5.11E-07 -0.016 -5.09E-07 -0.0801 -4.64E-07 0.2388 -1.36E-07 0.1747 -2.00E-07 0.1106 -3.55E-07 0.0465 -5.13E-07 -0.0176 -5.08E-07 -0.0816 -4.66E-07 0.2373 -1.37E-07 0.1732 -2.02E-07 0.1091 -3.60E-07 0.045 -5.14E-07 -0.0191 -5.07E-07 -0.0832 -4.63E-07 0.2357 -1.38E-07 0.1717 -2.05E-07 0.1076 -3.65E-07 0.0435 -5.16E-07 -0.0206 -5.06E-07 -0.0847 -4.64E-07 0.2342 -1.39E-07 0.1701 -2.07E-07 0.106 -3.70E-07 0.042 -5.17E-07 -0.0221 -5.05E-07 -0.0862 -4.57E-07 0.2327 -1.40E-07 0.1686 -2.10E-07 0.1045 -3.75E-07 0.0404 -5.18E-07 -0.0237 -5.04E-07 -0.0877 -4.74E-07 0.2312 -1.41E-07 0.1671 -2.12E-07 0.103 -3.80E-07 0.0389 -5.19E-07 -0.0252 -5.03E-07 -0.0893 -4.29E-07 0.2296 -1.42E-07 0.1656 -2.15E-07 0.1015 -3.85E-07 0.0374 -5.20E-07 -0.0267 -5.02E-07 -0.0908 -5.02E-07 0.2281 -1.43E-07 0.164 -2.18E-07 0.1 -3.89E-07 0.0359 -5.21E-07 -0.0282 -5.01E-07 -0.0923 -4.26E-07 0.2266 -1.44E-07 0.1625 -2.21E-07 0.0984 -3.94E-07 0.0343 -5.22E-07 -0.0298 -5.00E-07 -0.0938 -5.11E-07 0.2251 -1.45E-07 0.161 -2.24E-07 0.0969 -3.99E-07 0.0328 -5.22E-07 -0.0313 -4.99E-07 -0.0954 -4.03E-07 y -0.0389 -4.94E-07 x y -4.92E-07 -4.90E-07 -4.72E-07 -4.69E-07 -0.0969 -5.24E-07 -0.0984 -4.08E-07