Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)

Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)Tái sinh cây dâu tây (Fragaria Vesca L.) in vitro từ mảnh lá (Khóa luận tốt nghiệp)

i Tên TÁI SINH CÂY DÂU TÂY (Fragaria Vesca L.) IN VITRO : Chính quy Chuyên ngành Khoa : CNSH - CNTP : 2011 - 2015 ii Tên TÁI SINH CÂY DÂU TÂY (Fragaria Vesca L.) IN VITRO : Chính quy Chuyên ngành Khoa : 43 CNSH : CNSH - CNTP : 2011 - 2015 i Em S Thái Nguyên, ngày tháng ii 2012 12 nuôi 23 trình .24 P .26 27 (sau theo dõi) 29 (sau .30 nhân .32 33 iii Hình 2.1 Cây dâu tây .23 25 26 Hình 4.4 28 (sau t theo dõi) 30 (sau .31 dâu tây 32 34 iv CT : TN MS : Murashinge and Skoog, 1962 BAP : 6-benzylaminopurine Kinetin : 6-furfuryaminopurine NAA : Naphlene acetic acid IAA : 3-Indol acetic acid IBA : Indole-3-butyric acid v : .2 .2 .2 .2 : 2.1 2.2 2.3 .4 2.3 2.3 .5 2.3.4 .6 2.3.5 2.4 Tì 2.4.1 T .10 11 13 : 14 14 3.1.1 .14 14 14 vi 14 14 14 3.4 14 3.5 nghiên 15 3.6 15 .15 16 3.6.3 P 17 .18 3.6.5 dâu tây 19 3.6.6 20 3.7 21 : .23 nuôi c 23 4.2 .24 4.3 P 26 27 29 tr : 33 35 35 .35 36 1 (2004) [8], dâu tây l , [48] [8], [45] : [45], [37], [37] [37] [20], [22] [22] , [22], n [37], Tái sinh dâu tây (Fragaria Vesca L Tái sinh thành công dâu tây (Fragaria Vesca L.) in vitro 1.2.2 - P ng - P - P 1.3 1.3 1.3 in vitro tái sinh 35 agar 5,0g/l + Myo Inositol 100mg/l, pH= 5,61 o cao (91,3%) cao 72,0% - - BAP 0,2 1,0mg/l cho 80,0% 36 2009), Thái (2008), Selence & Technology Development, 11 in vitro ex vitro dâu tây (Fragaria x Ananassa Duch.), , 6: 233-239 Fragaria x ananassa Duch , , 2: 227-234 Fragaria vesca L., 10 11 37 12 bào 13 in vitro, 14 , 15 Barceló M., El-Mansouri I., Mercado J.A., Quesada M.A and Alfaro F.P (1998), Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler, Plant Cell, Tissue and Organ Culture, 54:29 36 16 Cao G., Russell R.M., Lischner N and Prior R.L (1998), Serum antioxidant capacity is increased by consumption of strawberries, spinach, red wine or vitamin C in elderly women, Journal of Nutrition, 128:2383 2390 17 Chevallier A (1996), The Encyclopedia of Medicinal Plants, Darling Kindersley Lim- ited, London 18 Chien-Ying K., Al-Abdulkarim A.M, Al-Jowid S.M and Al-Baiz A (2009), An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry, African Journal of Biotechnology, 8:2611-2615 19 Davis P.H (Ed.) ( 1972), Flora of Turkey and the East Aegean Islands, Edinburgh University Press 20 Debnath S.C (2005), Strawberry sepal: another explant for thidiazuroninduced adventitious shoot regeneration, In Vitro Cell Dev Biol Plant, 41:671-676 21 Dobelis I.N (1990), Magic and Medicine of Plants Association, New York 38 22 Foucault C., Letouze R (1987), In vitro regeneration de plantes de Fraisier a partir de fragmentes de petiole et de bourgeons floraux, Biol Plant, 29:409-414 23 Grieve M (Ed.) (1982), A Modern Herbal, 2, Dover Publications, New York 24 Hancock J.F (1999), Strawberries, CABI Publishing, New York 25 Heywood V.H., Brummitt R.K., Culham A., Seberg O ( 2007), Flowering Plant Families of the World, Firefly Books, Ontario, Canada 26 Howell A.B (2002), Cranberry proanthocyanidins and the maintenance of urinary tract health, Critical Reviews in Food Science Nutrition, 42:273 278 27 Kiselova Y., Ivanova D., Chervenkov T., Gerova D., Galunska B and Yankova T (2006), Correlation betweenthe in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs, Phytotherapy Research, 20:961 965 28 Kanodia L., Das S (2008), A comparative study of analgesic property of whole plant and fruit extracts of Fragaria vesca in experimental animal models, Bangladesh Journal Pharmacology, 4:35 38 29 Lamari Z., Landsberger S., Braisted J., Neggache H and Larbi R (2008), Trace element content of medicinal plants from Algeria, Journal of Radioanalytical Nuclear Chemistry, 276 (1):95 99 30 Mahmoud O., Kosar M (2013), Regeneration and Histological of Plants Derived from Leaf explants in vitro Culture of Strawberry, International Journal of Agriculture and Crop Sciences;943:950 31 Marta A.E., Camadro E.L., Diaz-Richi J.C and Castagnaro A.P (2004), Breeding berri- ers between the cultivatedstrawberry, Fragaria × ananassa, and relatedwild germplasm, Euphytica, 136:139 150 32 Media J.J., Clavero-Ramirez I., Gonzalez-Benito M.E (2007), Field performance characterization of strawberry (Fragaria × ananassa Duch.) 39 plants derived from cryopreserved apices, Scientia Horticulturae, 113:2832 33 Mozafari A., Gerdakaneh M (2012), Influence of media and growth regulators on regeneration and morphological characteristics of strawberry cvs Kurdistan and Merck (Fragaria x ananassa Duch.), International Journal of Plant Physiology and Biochemistry, 4(5):99-104 34 Mudnic I., Modun D., Brizic I., Vukovic J., Generalic I., Katalinic V., Bilusic T., Ljubenkov I and Boban M (2009), Cardiovascular effects in vitro of aqueousextract of wild strawberry (Fragaria vesca L.) leaves, Phytomedicine, 16:462 469 35 Naemura A., Mitani T., Ijiri Y., Tamura Y., Yamashita T., Okimura M., Yamamoto J (2005), Anti-thrombotic effect of strawberries, Blood Coagulation & Fibrinolysis, 16:501 509 36 Nehra N.S., Stushnoff C., Kartha K.K (1988), Regeneration of plants from immature, leaf-derived callus of strawberry, HortScience, 23:756 37 Owen H.R., Miller A.R (1996), Haploid plant regeneration from anther cultures of three north american cultivars of strawberry (Fragaria× ananassa Duch.), Plant Cell Rep, 15:905-909 38 Passey A.J., Barrett K.J., Jame D.J (2002), Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types, Plant Cell Rep, 21:397-401 39 Pawlaczyk I., Czerchawski L., Pilecki W., Lamer-Zarawska E.and Gancarz R (2009), Polyphenolic-polysaccharide compounds froms elected medicinal plants of Asteraceae and Rosaceae families: chemical characterization and bloodantico- agulant activity, Carbohydrate Polymers,77:568 575 40 Rugini E., Orlando R (1992), High efficiency shoot regeneration from callus of strawberry (Fragaria × ananassa Duch.) stipules of in vitro cultures, The Journal of Horticultural Science and Biotechnology, 67:577-82 40 41 Wang S.Y., Jiao H (2000), Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen, Journal of Agricultural and Food Chemirs, 48:5677 5684 42 Yildirim A.B., Turker A.U (2014), Effects of regeneration enhancers on micro propagation of Fragaria vesca L and phenolic content comparison of field-grown and in chromatography-electrospray vitro-grown tandem plant mass materials spectrometry by liquid (LC ESI- MS/MS), Scientia Horticulturae 169:169 178 43 Youssief E.Y (2009), In Vitro, Propagation of Strawberry (Fragaria × annanasa Duch.), Through Organogenesis via Runner Tips, 44:49 44 Yurdugul S (2008), An evaluation of the retention of quality characteristics in fresh and freeze-dried alpine strawberries, International Journal of Food Science & Technology, 43:865 870 45 Zakaria H., Hussein G.M., Abdel Hadi A.H.A., and Abdallah N.A (2014), Improved regeneration and transformation protocols for three strawberry cultivars, GM crops & Food: Biotechnology in agriculture and the Food chain, 5(1):27 35 rang web 46 FAOSTAT (2010), http://faostat.fao.org 47 FAOSTAT (2012), http://faostat.fao.org 48 http://gso.gov.vn/default.aspx?tabid=621&ItemID=13843 Nhóm I II III IV NH4NO3 1650 KNO3 1900 MgSO4 7H2O 370 MnSO4 H2O 22.3 ZnSO4 7H2O 10.6 CuSO4 H2O 0.025 CaCl2 2H2O 440 KI 0.83 CoCl2 6H2O 0.025 KH2PO4 170 H3BO3 6.2 Na2M0O4 2H2O 0,25 FeSO4 7H2O 27.85 Na2EDTA 2H2O 37.25 V mg/l Nicotinic acid Vitamins Glycine 0,5 Thiamine HCl 0,1 Pyridoxine HCl 0,5 BALANCED ANOVA FOR VARIATE NAYMAM FILE PAGE thi nghiem 1: khan nang mam cua hat 24/ 5/** 22: VARIATE V003 NAYMAM LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1120.67 1120.67 840.46 0.000 * RESIDUAL 5.33356 1.33339 * TOTAL (CORRECTED) 1126.00 225.200 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 24/ 5/** 22: PAGE thi nghiem 1: khan nang mam cua hat MEANS FOR EFFECT CT$ CT$ NOS NAYMAM 7.33333 34.6667 SE(N= 3) 0.666681 5%LSD 4DF 2.61324 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 24/ 5/** 22: PAGE thi nghiem 1: khan nang mam cua hat F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 6) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | NAYMAM 21.000 15.007 1.1547 5.5 0.0002 | BALANCED ANOVA FOR VARIATE MS FILE 25/ 5/** 16:27 PAGE thi nghiem 3: anh huong cua dieu kien sang/toi den kha nang tao mo seo VARIATE V003 MS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 394.889 197.444 55.53 0.000 * RESIDUAL 21.3334 3.55556 * TOTAL (CORRECTED) 416.222 52.0278 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 25/ 5/** 16:27 PAGE thi nghiem 3: anh huong cua dieu kien sang/toi den kha nang tao mo seo MEANS FOR EFFECT CT$ CT$ NOS MS 75.3333 81.0000 91.3333 SE(N= 3) 1.08866 5%LSD 6DF 3.76586 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 25/ 5/** 16:27 PAGE thi nghiem 3: anh huong cua dieu kien sang/toi den kha nang tao mo seo F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 9) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | MS 82.556 7.2130 1.8856 2.3 0.0003 | P BALANCED ANOVA FOR VARIATE MS FILE 25/ 5/** 17:59 PAGE thi nghiem 1: tao mo seo VARIATE V003 MS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 2479.60 619.900 464.92 0.000 * RESIDUAL 10 13.3334 1.33334 * TOTAL (CORRECTED) 14 2492.93 178.067 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 25/ 5/** 17:59 PAGE thi nghiem 1: tao mo seo MEANS FOR EFFECT CT$ CT$ NOS MS 0.000000 32.3333 36.0000 29.3333 21.0000 SE(N= 3) 0.666668 5%LSD 10DF 2.10070 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 25/ 5/** 17:59 PAGE thi nghiem 1: tao mo seo F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | MS 15 23.733 13.344 1.1547 4.9 0.0000 | c 4: tái sinh BALANCED ANOVA FOR VARIATE TS FILE 27/ 5/** 0:44 PAGE Thi nghiem 3: Anh huong cua BAP kêt hop voi TDZ den qua trinh tai sinh choi tu m VARIATE V003 TS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 338.267 84.5667 317.13 0.000 * RESIDUAL 10 2.66667 266667 * TOTAL (CORRECTED) 14 340.933 24.3524 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 27/ 5/** 0:44 PAGE Thi nghiem 3: Anh huong cua BAP kêt hop voi TDZ den qua trinh tai sinh choi tu m MEANS FOR EFFECT CT$ CT$ NOS TS 5.00000 7.66667 13.6667 11.3333 18.6667 SE(N= 3) 0.298142 5%LSD 10DF 0.939457 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 27/ 5/** 0:44 PAGE Thi nghiem 3: Anh huong cua BAP kêt hop voi TDZ den qua trinh tai sinh choi tu m F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | TS 15 11.267 4.9348 0.51640 4.6 0.0000 | BALANCED ANOVA FOR VARIATE NN FILE 26/ 5/** 2: PAGE thi nghiem 5: Anh huong cua BAP den qua trinh nhan nhanh choi VARIATE V003 NN LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 5788.27 1447.07 442.97 0.000 * RESIDUAL 10 32.6672 3.26672 * TOTAL (CORRECTED) 14 5820.93 415.781 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 26/ 5/** 2: PAGE thi nghiem 5: Anh huong cua BAP den qua trinh nhan nhanh choi MEANS FOR EFFECT CT$ CT$ NOS NN 20.6667 76.6667 70.6667 63.0000 59.3333 SE(N= 3) 1.04351 5%LSD 10DF 3.28813 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 26/ 5/** 2: PAGE thi nghiem 5: Anh huong cua BAP den qua trinh nhan nhanh choi F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | NN 15 58.067 20.391 1.8074 3.1 0.0000 | BALANCED ANOVA FOR VARIATE NN FILE 26/ 5/** 1:50 PAGE thi nghiem 6: Anh huong cua Kinetin den qua trinh nhan nhanh choi VARIATE V003 NN LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 5009.73 1252.43 347.89 0.000 * RESIDUAL 10 36.0003 3.60003 * TOTAL (CORRECTED) 14 5045.73 360.410 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 26/ 5/** 1:50 PAGE thi nghiem 6: Anh huong cua Kinetin den qua trinh nhan nhanh choi MEANS FOR EFFECT CT$ CT$ NOS NN 21.3333 72.3333 67.0000 64.6667 59.0000 SE(N= 3) 1.09545 5%LSD 10DF 3.45180 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 26/ 5/** 1:50 PAGE thi nghiem 6: Anh huong cua Kinetin den qua trinh nhan nhanh choi F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | NN 15 56.867 18.984 1.8974 3.3 0.0000 | BALANCED ANOVA FOR VARIATE NN FILE 26/ 5/** 1:42 PAGE thi nghiem 7: Anh huong cua Kinetin ket hop voi TDZ den qua trinh nhan nhanh cho VARIATE V003 NN LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 5282.27 1320.57 990.44 0.000 * RESIDUAL 10 13.3331 1.33331 * TOTAL (CORRECTED) 14 5295.60 378.257 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 26/ 5/** 1:42 PAGE thi nghiem 7: Anh huong cua Kinetin ket hop voi TDZ den qua trinh nhan nhanh cho MEANS FOR EFFECT CT$ CT$ NOS NN 21.3333 65.6667 68.6667 73.6667 57.6667 SE(N= 3) 0.666660 5%LSD 10DF 2.10067 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 26/ 5/** 1:42 PAGE thi nghiem 7: Anh huong cua Kinetin ket hop voi TDZ den qua trinh nhan nhanh cho F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | NN 15 57.400 19.449 1.1547 2.0 0.0000 | BALANCED ANOVA FOR VARIATE RR FILE 26/ 5/** 12:10 PAGE thi nghiem 8: Anh huong IBA den qua trinh re VARIATE V003 RR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 4523.60 1130.90 376.97 0.000 * RESIDUAL 10 29.9998 2.99998 * TOTAL (CORRECTED) 14 4553.60 325.257 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE 26/ 5/** 12:10 PAGE thi nghiem 8: Anh huong IBA den qua trinh re MEANS FOR EFFECT CT$ CT$ NOS RR 59.3333 65.0000 82.3333 95.3333 105.000 SE(N= 3) 0.999996 5%LSD 10DF 3.15102 ANALYSIS OF VARIANCE SUMMARY TABLE FILE 26/ 5/** 12:10 PAGE thi nghiem 8: Anh huong IBA den qua trinh re F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ (N= 15) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | RR 15 81.400 18.035 1.7320 2.1 0.0000 | ... 2006 [48] Do lúc n in vitro 8] Bê CO2 dâu tây [7], [8], [9], [13] [32] in vitro e 14 3.1.1 dâu tây g Tái sinh dâu tây in vitro dâu tây (Fragaria Vesca L.) phòng thí - in vitro - 12h/ngày -... (2004) [8], dâu tây l , [48] [8], [45] : [45], [37], [37] [37] [20], [22] [22] , [22], n [37], Tái sinh dâu tây (Fragaria Vesca L Tái sinh thành công dâu tây (Fragaria Vesca L.) in vitro 1.2.2... này, Passey (2002) ] in vitro -Ying K (2009) [18], in vitro cây in vitro [32] 2.4.2 loài khôn khác n 11 Tây Nguyên dâu tây, dân [13] in vitro [7] Fragaria x ananassa Duch n in vitro ng g/l, saccarose