microcarpa Hassk Quýt Sài gòn M1 2 Citrus nobinis var.. chryoscarpa Lamk Quýt Thái Lan M2 3 Citrus japonica Thunb.. Cam Vinh M8 9 Citrus aurantifolia Sw.. 1998 Tocopherol and tocotrie
Trang 2
c chi Citrus
Trang 4
2
7
8
9
10
- 12
CITRUS 12
aceae 12
Citrus 13
19
20
1.2.2 Các tocopherol v 28
32
1 36
- 39
39
39
39
40
- phân tích lipid, tocopherol và axit béo 40
Trang 5 41
43
Citrus japonica Thunb 43
44
- 45
45
45
3.1.2 45
3.1.3 K 45
46
3.2.1 in vitro -diphenyl-1-picryldydrazyl) 46
in vitro 48
3.2.3 Total Oxyradical Scavenging Capacity Assay) 49 51
3.3.1 51
52
54
54
Trang 6 - 57
57
CITRUS 60
60
4.2.2 62
65
65
ng 69
76 4.4 80
80
80
81
83
Trang 7
PUFA Polyunsaturated fatty acids
POL Peroxy hóa lipid
AND Acid Deoxyribo Nucleic
HPLC High-performance liquid chromatography
ELC Equivalent Chain- lengths
DPPH 2,2-diphenyl-1-picryldydrazyl
ABAP -Azobis(2-methylpropionamidine) dihydrochloride
KMBA -Keto - -(methylthio)butyric acid
TLC Thin layer chromatography
TOSCA Total Oxyradical Scavenging Capacity Assay
SC50 Scavenging capacity 50%
LD50 Lethal dose 50%
ED50 Effective dose 50%
Trang 8 - Các axit béo no 21
- 23
- 25
B - 26
- Các axit acetylenic 27
- Danh 39
- 55
- o và tocopherol 58
58
- 59
59
- 60
- 61
61
- 62
- 64
64
- 65
- 66
- 66
- 69
- 70
- 73
- 73
- (Thunb.) Swingle tr 79
- 80
Trang 9 - 12
- 13
- Flavonoit 18
- -tocopherol 31
Hình 3.1 - 47
- 63
63
- 63
63
- 65
66
67
Hình 4.6 - 68
Hình 4.7 - 71
Hình 4.8 - 72
Hình 4.9 - 72
Hình 4.10 - 75
Hình 4.11 - 76
Hình 4.12 - 77
Hình 4.13 - 78
Trang 10
Database for New Crop Seeds) -
Trang 11 axit béo omega6 , c tocopherol T các k t qu nghiên c u s ác
b ên, ctr nh các cây thu c chi Citrus Vi Nam
Trang 19
Trang 20 enoic và alkyn thành alkynoic
[18]
Trang 21 1.1 - Các axit béo no
Hexadecanoic CH3(CH2)14-COOH 16:0 Palmitic
Heptadecanoic CH3(CH2)15-COOH 17:0 Margaric
Octadecanoic CH3(CH2)16-COOH 18:0 Stearic
Nonadecanoic CH3(CH2)17-COOH 19:0
Eicosanoic CH3(CH2)18-COOH 20:0 Arachidic
Docosanoic CH3(CH2)20-COOH 22:0 Behenic
Tetracosanoic CH3(CH2)22-COOH 24:0 Linoceric
Hexacosanoic CH3(CH2)24-COOH 26:0 Cerotic
Octacosanoic CH3(CH2)26-COOH 28:0 Montanic
Triacontanoic CH3(CH2)28-COOH 30:0 Melissic
Dotriacontanoic CH3(CH2)30-COOH 32:0 Lacceroic
Trang 23 -
cis 9- Octadqecenoic 18:1 9c Asclepic or cis-vaccenic
trans-11- Octadecenoic 18:1 11t Gadoleic
cis-13-Docosenoic 22:1 13c Selacholeic or nervonic
cis-19-Nonacosenoic 26:1 19c
Axit oleic
axit oleic cao (72 ± ±
±
80% -
Trang 24-nhi ên [59] Axit vaccenic (11c-18:1)
Trang 258c,10t,12c- Octadecatrienoic Jacaric Jacaranda minosifolia
8t,10t,12c- Octadecatrienoic Calendic Calendula officinalis
8t,10t,12t- Octadecatrienoic Calendula officinalis
9c,11t,13c- Octadecatrienoic Catalpic Catalpa ovata
9c,11t,13t- Octadecatrienoic -Eleostearic Tung iol
9t,11t,13c- Octadecatrienoic Punicic Punica granatum
Trang 26 -
V
n-3
9,12,15- Octadecatrienoic 18:3(n-3) -Linolenic 6,9,12,15- Octadecatetraenoic 18:4(n-3) Stearidonic 5,8,11,14,17-Eicosapentaenoic 20:5(n-3) EPA
4,7,10,13,16,19-Docosahexaenoic 22:6(n-3) DHA
n-6
6,9,12-Octadecatrienoic 18:3(n-6) -Linolenic 5,8,11,14-Eicosatetraenoic 20:4(n-6) Arachidonic 7,10,13,16-Docosatetraenoic 22:4(n-6) Adrenic
- prostaglandin, thromboxan [32], [34]
Trang 27Các axit acetylenic trong
-C C-
thê
9c-Octadecene-12-ynoic 18:2 9c,12a Crepenynic
[9]
Trang 307,8 - dimetyl tocoltrienol ( -T 3)
8 - methyl tocotrienol ( -T 3)
- và - tocopherol - n/c = 2,5-3,50 C, không tan trong
Trang 31H3C R O
O
OH CH3
- e+ e
Trang 32 tocopherol acetat (este)
1
oxy
Trang 33
Trang 39 2.1
Citrus nobilis var microcarpa Hassk -12/2010
2 Citrus nobinis var chryoscarpa Lamk i 2-3/2011
4 Citrus grandis (L.) Osb.var.gradis -10/2010
5 Citrus grandis var.racemosa -3/2011
7 Citrus nobilis Lour var Nobinis -3/2011
9 Citrus aurantifolia Swingle -3/2011
Trang 40 - Hitachi F-1000 Fluorescence Spectrophotometer, 295/330nm, D-
- 1.3 ml/p
Trang 41(99+1,v/v (99+1,v/v).
-
5509:1997 (International Organisation for Standardisation/ Final draft) [42]
instrument Model 5890 Series II, CP--Sil 88, 100m /0.25mm/0.25C18:0) [17]
-220oC(1.5ooC/ phút, 260oC/5 phút; Split: 1:50; injector 250oC, detector 250oC, khí mang 36cm/s hydrogen, detector gas:
-2
2 diphenyl-1-picryldydrazyl)
lg RT 18:0 lgRT 16:0
Trang 42Haber-Weiss [4] 2O2 (hydrogen perox ) và id superoxid 2-
Trang 45 3.1
.1
3.1.2
3.1.1 Hoà tan 70mg-
-6000 Pump, Fluorescenz spectrophormeter 295/330nm, D-
Trang 46502SO4
-Sil 88, 100m/0.25mm/0.25
[17] 4.2,
3.2
CITRUS
3.2.1 in vitro 1-picryldydrazyl)
Trang 47
Hình 3.1 -
Trang 48- E (Minimal Essential Medium Eagle), trypsin,
MTT -(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-(3 tetrazolium- bromide),
Trang 49o
Mi
3
a TM STB-1 (30m x 0,32mm x 0,25µm)0C, injector: 1800C, detector FID 180: 0
b :