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CONTENT LIST OF TABLE i !"""#""$#%&''''''''''''''''' () !"#""*)#(&'''''''''''''''''' +, )#" )#%&''''''''''''''''''- $.''''''''''''/ ii Faculty of Agronomy Hanoi University of Agricultural Advance crop science program Progress reports: SUBJECT ADVANCED RESEARCH PROGRAM 2010-2011 I. Introduction , 0 Tomato yellow leaf curl . 1 2 ) ) %)2 3 ) ) 45)*&20- /,%)) 4Bemisia tabaci) " %) 62%)74)52 )4)522 8, .22) 4! 52 49! & 5 :49!: 562 &! ! & 2 )*) 2);2) 8"";2! ()2 < *&#%) 07“ Investigating,study, 1 diogonistic Begomovirus virus on Tomato= .2. Objectives and Requirements 6>7 #)%) .?7 # 8)) ( & #*)%) II. Literature review 2.1. Researches in the world. 2)%82""2@ ;;,2;) ,)2) #)))2) 2)2) 2.2. Host range ( ) %) 2 8 %)9! )-+ )A, 4;;54!5% !2!2!2! 9! )7B)2 $2$ )24&52>4*5 2.3. Symptoms 2 9#, ) ) 2 22)22 22)8) 2.4. Cause agent 8 """ 8 1 48 5 9! %))23 20 "#+" *& ) /""-)2#*& 7*&*&%2-""*& )2*&%) 2.5. Vector and transmitation of Begomovirus Bemisia tabaci 4(7 Aleyrodidae), 2 2 2)2,$,88 )2 ) -"" )) 2 82 2?)))CC2 #+#;28) 2)-D ;D"D.( +"#-"E6212 !,2Bemisia tabaci ,) 1)))00 ))2 +"" ) 0))72 )1 3 ) 9) #) )) ?)2) ))2 )))) ) III.Material and method 3.1. Location and duration of the study #)(&. # . $ $)2(F ) 3.2. Materials #$7 # ! 4 ( (2&*2$2()92(*)5 # ! 2 $. 2 ?2 # )2 2 )2# - .)G %*&1 • %HIJB • &6("J 3.3. Methodology 4 3.3.1. Field surveying )2) ) )7 *4) 5 3.3.2. Collecting and storing samples #)7) )7 H& H!2 H #)7)), H*)7))#)) H)7#" " 3.4 Research method 3.4.1. Survey methodology in prevalence and white fly density on the field #8))7"&##/$$* #CG)2))2"G) #$: ))2"G Ratio of disease 5 & $4E5KLLLLLLLLLL1"" ) White fly density: ) M4G5KLLLLLLLLLLL ) 3.4.2. DNA extraction *&17 (i) )% 7 # )")"") # )"%HIJB,-" ") # :-" " 7 # )"14775,) # )"""G"2,) 4"+5 +7 # $"+14775 ,) #)",) 4"5 6 7 # $$,#" " # )",)2*& 2-7.BC" 4"2CG52B ,1B C7*&+" /7**&",#" (ii) F)&6("J*&1 #") #"&6("J #*"#"""J2(K/ 3.5. PCR test7 3.5.1. PCR testing: N$. #3%)*&#%). O%)4(2""-5 %). 7<#(J*(:%3 N #+< %)7<#393.3339.39. N #+< N94G52.43G52(4GG52J4G52%4G3G52*4G3G52:43G5 K # ! 4K5 H*! 4K5 4!5 < 33333333+ < 4!C-5 HF! P.##7 7 4!-/+5 < 333+ < 4!C"/5 #9! & 4K+/-57 H*9! & ###?7 4!";5 < 3333+ < 4!C5 HF9! & #. # #?7 4!-5 < 3333+ < 4!/"5 #*&#I)%O%. %7<#3333333#+< %.7<#3333333333#+< $*&#I#0+""#"" 3.5.2. Conducting PCR: H8)$.7 ( 6 72Q *% 72"Q &$ 7"2+Q 7"2+Q . 7"2+Q *) 7"2Q *& 7"2Q 7Q H$)$. ; 1 ; +" 1+ 8 [...]... Molecular characterization of begomoviruses and DNA satellites from Vietnam: additional evidence that the New World geminiviruses were present in the Old World prior to continental separation Journal of General Virology 89: 312-326.) 11 Green, S K., Tsai, W S., Shih, S L., Black, L L., Rezaian, A., Rashid, M H., Roff, M M N., Myint, Y Y & Hong, L T A (2001) Molecular characterization of begomoviruses... prepared with TAE buffer and containing 0.5 mg / ml ethidium bromide * Gels were run on mobile devices is the Mini - Sub Cell (Biorad) with TAE buffer at 100 V voltage for 30 - 40 minutes * Gel examined under ultraviolet light and photographed using a dedicated camera or digital camera 9 Advance Crop Science 52 IV Result and discussion 4.1 The survey field 4.1.1 Describe the symptoms in the field In Vietnam,... 4.1% to 5.5% (up 1.4%) White fly density was irregular changing, too It was increase rapidly from branching to fruiting stage For Chanako variety was 0.62 individual/leaf and C155 variety was 0.60 individual /leaf There was two days white fly density decrease (10/02/2010 and 03/07/2011) because it was rain , temperature also decrease It made white fly could not fly into anywhere Through the survey... showed that 2/8 samples generated single tape and the size was about 1386 bp confirmed the presence of TYLCVNV Results of PCR tests found 6th samples (samples collected in Thieu Do- Thieu Hoa- Thanh Hoa) results (+) with both pairs of primer demonstrated both samples were infected with two viruses (TYLCVNV, ToLCVV) This result is not surprising because two or more mixed begomovirus on the same plan... the seedling stage with a low rate In particular, at the same 16 Advance Crop Science 52 stages infected Chanoka variety is higher than C155 In old stage, the disease rate is highest with both varieties: Chanoka was 10.2% and C115 was 8.0% Disease appears early in the final stages of seedlings with rate of 0.8% in Chanoka and 0.2% in C155 Prevalence of disease increased rapidly when plants entered a... sample 6: ( TH4) Thieu Do – Thieu Hoa – Thanh Hoa sample 7: ( ND) Truc Chinh – Truc Ninh – Nam Dinh sample 8: ( PT) Phu Tho Table 4.2 PCR test results tomato samples collected in the field 17 Advance Crop Science 52 No Sample’ name Bego TY To 1 2 3 4 5 6 7 8 HC1 HC2 TH1 TH2 TH3 TH4 ND PT + + + + - + + - + + + - We used pairs of general primer for DNA-A of different species of Begomovirus (BegoAFor1/BegoARev1)... to detect the sample To check the presence of begomovirus and 2 viruses ToLCVV and TYCVNV in 8 tomatoes samples which bear yellow leaf curl symptom collected in Thanh Hoa, Nam Dinh, Phu Tho, and HaNoi University of Agricultural Using (BegoAFor1/BegoARev1) showed that 4/8 samples created single tape and the size was about 1200 bp determine the existence of Begomovirus As a results we used pairs of specific... (diseases caused by unfavorable environmental causes) Tomato disease with typical symptoms: curly top of the plant, causing curled leaves, stunted plant, flowers grow less, vulnerable to loss Infected at a young stage, it will be curly faster; plant can not grow fast, no flower to wither Based on the typical symptoms of the leaf culf disease are described in the virus literature, we conducted observations,... method to detect that disease on tomato and other plant Reference 1 Nguyen Tho (1984), Dieu tra nghiencuu mot so benh virus chu yeu cua ba cay trong ho ca ( salanaceae) co y nghia kinh te ; thuoc la , ca chua, khoai tay ) Luan an PTS, tr 45-72 2 VuTrieu Man (2003), Chan đoan nhanh benh hai thuc vat Agricultural published , HN 3 Cohen, S & Antignus, Y (1994) Tomato yellow leaf curl virus, a whitefly-borne... in VL2000 than F1 hybrid White fly density increased from branching stage and highest in the greening fruit stage It was small rain in 15th and 16 th October, white fly density has reduced on the next day of survey (0.46 individual/ leaf) 31/10/2010 density of White fly 0.78 individual/leaf in F1 and 0.80 like VL2000 Thank to reduced of temperature density of white fly on the two varieties were dropped . ii Faculty of Agronomy Hanoi University of Agricultural Advance crop science program Progress reports: SUBJECT ADVANCED RESEARCH PROGRAM 2010-2011 I. Introduction , 0 . ) /""-)2#*& 7*&*&%2-""*& )2*&%) 2.5. Vector and transmitation of Begomovirus Bemisia tabaci 4(7 Aleyrodidae), 2 2. and VL101 in fall-winter 2010 in Dong Anh – Ha Noi Table 4.1.2.2. Tomato yellow leaf curl and white fly density developmenmt on Hong Chau and VL101 in fall-winter 2010 in Dong Anh – Ha Noi 13 No