Xây dựng quy trình nhân nhanh in vitro cây bẫy kẹp b52 (dionaea muscipula)

KHOA IN VITRO B52 (Dionaea muscipula) n : K63-CNSHB : 637136 -2022 T i xin m o n ề t i n y o t i th u qu tr nh n y l trung th h h hi n S li u v kết qu nghi n t ng s ng v ng t v n o T i xin kh m o n m i s gi p ều m n v th ng tin tr h n lu n ều h r ngu n g ăm 2022 Sinh vi n th hi n i ho n th nh kh th n t i nh n nhi t t nh lu n t t nghi p n y ngo i s n l g ng n s qu n t m t o iều ki n thu n l i nh t t s gi p th y n v nh ng ng u ti n v i l ng k nh tr ng v n TS Nguy n Th L m H i m i iều ki n gi p tin t i th n gi iết n s u s nh t i xin h n th nh ng gi o ề t i t n t nh h t i su t th i gi n th ng m n v t o hi n v ho n th nh o o t t nghi p T i xin N m t o iều ki n ho t i th nhi m kho h m n h n th nh t i Th ng ngh sinh h v t t n t nh h su t th i gi n th n nh t i ng ngh sinh h th hi n kh Th y n h nh s v t i ho n th nh t t kh ng xin v t p th vi n N ng nghi p Vi t lu n t t nghi p ng ng t p n qu n l H gi p m n n h ng ngh sinh sung kiến th ho t i lu n t t nghi p m n ến to n th n m n ph ng n kho ng vi n t i qu tr nh t p t t nghi p T i ng xin gian ch b o h gi p y tỏ l i c m n s u s ng d n v ng S n nh th i hi sẻ kiến th c kinh nghi m xu t ph t t th c ti n, t i su t qu tr nh th c t p Do th i gi n v iều ki n h n n n ề t i t t nghi p c c nh ng thiếu s t k nh mong th y lu n ến nh V v n ng g p em kh ng tr nh kiến b n kho ho n thi n h n T i xin h n th nh m n ăm 2022 Sinh vi n ii L I M O N i L I M N ii DANH MỤC B NG vi DANH MỤ HÌNH NH vii DANH MỤC VIẾT TẮT viii T M TẮT ix Ph n I: M 11 u t v n ề 12 M ti u 13 Y u ề t i u Ph n II: T ng Qu n T i Li u 2.1 Gi i thi u chung h t m i Droseraceae) 1 Gi i thi u h tm i roser e e 2 Ph n lo i 2 Gi i thi u 2 Ngu n g y y kẹp B52 (Dionaea muscipula) 2 Ph n lo i 223 i m th 2 H nh th v th y y kẹp 52 sinh s n 2 Ph ng th t m i 23 Y u u ngo i nh Gi tr y M t s nghi n u chi Dionaea Tr n gi i T i Vi t N m 12 PH N III: V T LI U N I UNG V PH NG PH P NGHI N U 14 iii Th i gi n v i m nghi n V t li u nghi n u 14 3 Ph u 14 ng ph p nghi n u 14 3 Ph ng ph p nu i y invitro 14 3 Ph ng ph p 3 Ph ng ph p x l s li u 15 N i ung nghi n tr th nghi m 15 u 15 Nghi n u s nh n nh nh in vitro Nghi n us r r 35 h ti u theo y 411 nh h nh h ng nh h m i tr nh h nh h y y kẹp i 21 y ng c a kinetin ến s nh n nh nh h i y kẹp 52 s u y y kẹp 52 i 27 ng c a kinetin +IAA ến s nh n nh nh h i 52 s u tu n theo 415 ng ến s ph t tri n i 24 (sau tu n theo 414 u V Th o Lu n 21 ng c a BA ến s nh n nh nh h i tu n theo 4.1.3 y kẹp 52 18 u nh n nh nh h i 21 B52 (sau tu n theo 4.1.2 y kẹp 52 15 i 19 Ph n IV: Kết Qu Nghi n Nghi n y y kẹp i 30 ng c a kinetin+ IBA ến s nh n nhanh ch i B52 ( sau tu n theo y y y kẹp i 34 Nghi n u s r r 37 421 nh h ng c a IAA ến s r r y s u tu n theo i 37 422 nh h ng c a IBA ến s r r y s u tu n theo i 39 423 nh h ng th n ho t t nh ến s r r y s u tu n theo i 40 PH N V: KẾT LU N V Ề NGH 43 iv Kết lu n 43 52 ề ngh 43 T I LI U TH M KH O 45 PHỤ LỤ 48 v DANH M C B NG B ng M t s enzyme ung B ng nh h ng m i tr kẹp 52 s u tu n theo h ti u h y n th t ng ến s ph t tri n ng c a BA ến s nh n nh nh h i B ng 4.3 nh h ng c a kinetin ến s nh n nh nh h i B ng 4 nh h nh h ng kinetin I nh h y kẹp 52 24 y y kẹp 52 ến s nh n nh nh h i y y i 31 ng c a kinetin+ IBA ến s nh n nh nh h i kẹp 52 s u tu n theo B ng y i 27 kẹp s u tu n theo B ng y i 22 B ng 4.2 nh h s u tu n theo y y i 34 ng c a IAA ến s r r I y ến s r r y s u tu n theo i 37 y s u tu n theo i 39 B ng 4.7 nh h ng c B ng 4.8 nh h ng c a th n ho t t nh ến s r c y 41 vi DANH M NH H nh Dionaea muscipula B52 H nh h i y y kẹp invitro tr n m i tr ng sung h m l ng kho ng MS s u tu n 23 H nh h i kh H nh kh y y kẹp invitro tr n m i tr ng sung n ng nh u s u tu n 26 h i y y kẹp tr n m i tr ng sung kinetin n ng nh u s u tu n 30 H nh 4 h i n ng H nh y kh h i y kẹp tr n m i tr ng sung kinetin I nh u s u tu n 33 y y kẹp tr n m i tr ng sung Kinetin I n ng kh H nh R y y kẹp tr n m i tr ng sung I s u tu n 38 H nh R y y kẹp tr n m i tr ng sung I s u tu n 40 H nh R n ng y kh nh u s u tu n 36 y kẹp tr n m i tr ng sung th n ho t t nh nh u s u tu n 42 vii DANH M C VIẾT TẮT BA CT Benzyl adenine ng th Cm Centimet CV% S i s th nghi m i h ng G/l Gam/lit KI Kinetin LSD 0,05 S i s nhỏ nh t Mg/l Milig m/l t MS M i tr IAA Indole-3-acetic acid IBA Indole-3-butyric acid ngh m ng Mur shige n Skoog 1962 viii Ắ ề t i X Y NG QUY TRÌNH NH N NH NH IN VITRO Y K P B52 (Dionaea muscipula nh n nh nh in vitro y th p Kết qu nghi n M i tr MS 30g/L h i trung y kẹp t i u h quy tr nh ho h s nh n o hi ph u ho th y: y 6g/L g r pH v i s l trung y kẹp invitro l 1/2 nh l 15 43 hiều o nh 26 m s u tu n Kinetin I ng nh n nh nh h i t t nh t l m i tr ho h s nh n h i 0,6 mg/l Kinetin mg/l I nh M i tr t75l / h iv ng t o su rose g/l g r r trung hi n nh m ho n thi n quy tr nh ng th h h p ho s ph t tri n ng M i tr l trung th Y nh thu ng sung kết h p o nh t: 1/2 MS 30g/l su rose g/l g r pH ho h s nh n h i l h i/m u s hiều o thu l 35 m s u tu n y ho n ch nh t t nh t l m i tr ng 1/2 MS 30g/l g/l th n ho t t nh, pH= 5,8 ho t l r r t 83 r /m u v ho hiều i r trung t 100 s nh l 39 cm sau tu n ix 14 Phytochemistry 41:775–778 Parliman BJ, Evans PT, Rupert EA (1982a) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex L., the Venus fly-trap, for rapid asexual propagation 15 J Am Soc Hort Sci 107:305–310 Parliman BJ, Evans PT, Mazur AR (1982b) Adventitious bud differentiation and development in leaf cuttings of Dionaea muscipula Ellis ex L (Venus fly trap) cultured in vitro 16 J Am Soc Hort Sci 107:310–316 Perica MC, Berljak J (1996) In vitro growth and regeneration of Drosera spathulata Labill https://vovankienthuc.com/blog/cay-bat-ruoi-dionaea.10 https://vi.wikide2.com/wiki/Venus_flytrap https://www.sbc-vietnam.com/san-pham/hoa-chat-dung-cu-nuoi-cay-mo-thucvat/vi-nhan-giong-cay-bay-ruoi-venus-bang-nuoi-cay-cho.aspx 4.https://codai.net/tra-cuu-ten-khoa-hoc-cua-cac-giong-lai-tao-thuoc-chidionaea-chi-bay-kep-bang-hinh-anh/ https://en.wikipedia.org/wiki/Droseraceae 47 Th nghi m 1: nh h (s u tu n theo ng c m i tr ng ến s ph t tri n y y kẹp B52 i BALANCED ANOVA FOR VARIATE CCAO FILE TN1 23/ 8/** 15:58 PAGE thi nghiem VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 5.75393 1.91798 ****** 0.000 NL 620000E-03 310000E-03 0.35 0.713 * RESIDUAL 114 101838 893317E-03 * TOTAL (CORRECTED) 119 5.85639 492134E-01 BALANCED ANOVA FOR VARIATE SLTB FILE TN1 23/ 8/** 15:58 PAGE thi nghiem VARIATE V004 SLTB LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 563.292 187.764 396.52 0.000 NL 1.05000 525000 1.11 0.334 * RESIDUAL 114 53.9831 473536 * TOTAL (CORRECTED) 119 618.325 5.19601 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN1 23/ 8/** 15:58 PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 CCAO 0.655667 1.25933 1.06733 0.948667 SLTB 9.56667 15.4333 13.7000 12.0000 SE(N= 30) 0.545685E-02 0.125636 5%LSD 114DF 0.152860E-01 0.351939 MEANS FOR EFFECT NL NL NOS CCAO SLTB 40 0.980250 12.7750 40 0.985750 12.5500 40 0.982250 12.7000 48 SE(N= 40) 0.472577E-02 0.108804 5%LSD 114DF 0.132380E-01 0.304788 - ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN1 23/ 8/** 15:58 PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 120) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CCAO 120 0.98275 0.22184 0.29888E-01 3.0 0.0000 0.7125 SLTB 120 12.675 2.2795 0.68814 5.4 0.0000 0.3342 th nghi m tu n theo nh h ng |NL | ến s nh n nh nh h i y y kẹp B52 (s u i BALANCED ANOVA FOR VARIATE CCAO FILE TN2 23/ 8/** 19:54 PAGE thi nghiem VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 5.75595 1.15119 ****** 0.000 NL 101333E-02 506667E-03 0.52 0.603 * RESIDUAL 172 168461 979424E-03 * TOTAL (CORRECTED) 179 5.92542 331029E-01 BALANCED ANOVA FOR VARIATE SL FILE TN2 23/ 8/** 19:54 PAGE thi nghiem VARIATE V004 SL LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 93.0278 18.6056 94.62 0.000 NL 1.47778 738888 3.76 0.025 * RESIDUAL 172 33.8222 196641 * TOTAL (CORRECTED) 179 128.328 716915 BALANCED ANOVA FOR VARIATE HS FILE TN2 23/ 8/** 19:54 PAGE thi nghiem VARIATE V005 HS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER 49 SQUARES SQUARES LN ============================================================================= CT 527.466 105.493 527.46 0.000 NL 133333 666663E-01 0.33 0.722 * RESIDUAL 172 34.4005 200003 * TOTAL (CORRECTED) 179 562.000 3.13966 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN2 23/ 8/** 19:54 PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CCAO 1.18033 1.20100 1.30967 1.48167 1.70367 1.36967 SL 4.06667 4.50000 5.00000 5.70000 6.16667 5.53333 HS 2.50000 5.50000 6.33333 6.80000 7.76667 7.10000 SE(N= 30) 0.571379E-02 0.809611E-01 0.816502E-01 5%LSD 172DF 0.159479E-01 0.225973 0.227896 MEANS FOR EFFECT NL NL NOS CCAO SL 60 1.37767 5.28333 60 1.37233 5.13333 60 1.37300 5.06667 HS 6.00000 6.03333 5.96667 SE(N= 60) 0.404026E-02 0.572481E-01 0.577354E-01 5%LSD 172DF 0.112769E-01 0.159787 0.161147 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN2 23/ 8/** 19:54 PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CCAO 180 1.3743 0.18194 0.31296E-01 2.3 0.0000 0.6028 SL 180 5.1611 0.84671 0.44344 8.6 0.0000 0.0248 HS 180 6.0000 1.7719 0.44722 7.5 0.0000 0.7219 Th nghi m (s u tu n theo nh h |NL | ng c a Kinetin ến s nh n nh nh h i y y kẹp B52 i BALANCED ANOVA FOR VARIATE CCAO FILE TN3 2/ 9/** 20:11 PAGE thi nghiem 50 VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 4.63999 927999 943.85 0.000 NL 619000E-02 309500E-02 3.15 0.044 * RESIDUAL 172 169112 983209E-03 * TOTAL (CORRECTED) 179 4.81530 269011E-01 - BALANCED ANOVA FOR VARIATE SL FILE TN3 2/ 9/** 20:11 PAGE thi nghiem VARIATE V004 SL LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 178.761 35.7522 199.08 0.000 NL 677778 338889 1.89 0.152 * RESIDUAL 172 30.8890 179587 * TOTAL (CORRECTED) 179 210.328 1.17502 - BALANCED ANOVA FOR VARIATE HS FILE TN3 2/ 9/** 20:11 PAGE thi nghiem VARIATE V005 HS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 583.161 116.632 634.39 0.000 NL 211111 105555 0.57 0.570 * RESIDUAL 172 31.6220 183849 * TOTAL (CORRECTED) 179 614.994 3.43572 - 51 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN3 2/ 9/** 20:11 PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CCAO 1.26000 1.60067 1.65633 1.74833 1.50833 1.41733 SL 3.96667 5.26667 6.16667 7.13333 6.30000 6.20000 HS 2.53333 6.80000 7.03333 7.80000 7.50000 7.30000 SE(N= 30) 0.572483E-02 0.773708E-01 0.782834E-01 5%LSD 172DF 0.159787E-01 0.215952 0.218499 MEANS FOR EFFECT NL NL NOS CCAO SL 60 1.52650 5.76667 60 1.52900 5.91667 60 1.54000 5.83333 HS 6.45000 6.50000 6.53333 SE(N= 60) 0.404806E-02 0.547094E-01 0.553547E-01 5%LSD 172DF 0.112987E-01 0.152701 0.154502 - 52 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN3 2/ 9/** 20:11 PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CCAO 180 1.5318 0.16402 0.31356E-01 2.0 0.0000 0.0443 SL 180 5.8389 1.0840 0.42378 7.3 0.0000 0.1523 HS 180 6.4944 1.8536 0.42878 6.6 0.0000 0.5698 Th nghi m nh h B52 s u tu n theo ng c a Kinetin I |NL | ến s nh n nh nh h i y y kẹp i BALANCED ANOVA FOR VARIATE CCAO FILE TN4 2/ 9/** 19:55 PAGE Th nghi?m VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 8.01529 1.60306 ****** 0.000 NL 176777E-02 883886E-03 0.89 0.414 * RESIDUAL 172 170067 988760E-03 * TOTAL (CORRECTED) 179 8.18712 457381E-01 - 53 BALANCED ANOVA FOR VARIATE SL FILE TN4 2/ 9/** 19:55 PAGE Th nghi?m VARIATE V004 SL LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 210.117 42.0233 243.92 0.000 NL 325114E-12 162557E-12 0.00 1.000 * RESIDUAL 172 29.6333 172286 * TOTAL (CORRECTED) 179 239.750 1.33939 - BALANCED ANOVA FOR VARIATE HS FILE TN4 2/ 9/** 19:55 PAGE Th nghi?m VARIATE V005 HS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 62.1611 12.4322 78.17 0.000 NL 2.14444 1.07222 6.74 0.002 * RESIDUAL 172 27.3556 159044 * TOTAL (CORRECTED) 179 91.6611 512073 - 54 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN4 2/ 9/** 19:55 PAGE Th nghi?m MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CCAO 1.29967 1.38967 1.80367 1.84833 1.77033 1.68300 SL 3.40000 5.10000 6.33333 6.60000 6.06667 5.00000 HS 6.73333 6.76667 7.30000 8.30000 7.90000 6.96667 SE(N= 30) 0.574096E-02 0.757818E-01 0.728112E-01 5%LSD 172DF 0.160237E-01 0.211516 0.203225 MEANS FOR EFFECT NL NL NOS CCAO SL 60 1.63150 5.41667 60 1.63667 5.41667 60 1.62917 5.41667 HS 7.46667 7.31667 7.20000 SE(N= 60) 0.405947E-02 0.535858E-01 0.514853E-01 5%LSD 172DF 0.113305E-01 0.149565 0.143702 - 55 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN4 2/ 9/** 19:55 PAGE Th nghi?m F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CCAO 180 1.6324 0.21386 0.31445E-01 1.9 0.0000 0.4136 SL 180 5.4167 1.1573 0.41507 7.7 0.0000 1.0000 HS 180 7.3278 0.71559 0.39880 5.4 0.0000 0.0017 th nghi m nh h B52 s u tu n theo ng c a Kinetin I |NL | ến s nh n nh nh h i y y kẹp i BALANCED ANOVA FOR VARIATE CCAO FILE TN5 2/ 9/** 20: PAGE thi nghiem VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 14.5660 2.91320 ****** 0.000 NL 233343E-04 116672E-04 0.29 0.753 * RESIDUAL 172 691986E-02 402318E-04 * TOTAL (CORRECTED) 179 14.5729 814130E-01 BALANCED ANOVA FOR VARIATE SL FILE TN5 2/ 9/** 20: PAGE thi nghiem VARIATE V004 SL LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 233.428 46.6855 198.49 0.000 NL 311111 155556 0.66 0.522 * RESIDUAL 172 40.4556 235207 * TOTAL (CORRECTED) 179 274.194 1.53181 BALANCED ANOVA FOR VARIATE HS FILE TN5 2/ 9/** 20: PAGE thi nghiem VARIATE V005 HS LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 113.311 22.6622 90.53 0.000 56 NL 211112 105556 0.42 0.662 * RESIDUAL 172 43.0556 250323 * TOTAL (CORRECTED) 179 156.578 874736 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN5 2/ 9/** 20: PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CCAO 1.52100 1.64900 2.34933 2.17533 1.98567 1.91567 SL 4.00000 6.16667 7.50000 7.20000 6.50000 5.80000 HS 7.26667 7.33333 9.00000 8.26667 7.76667 6.50000 SE(N= 30) 0.115804E-02 0.885451E-01 0.913461E-01 5%LSD 172DF 0.323224E-02 0.247140 0.254958 MEANS FOR EFFECT NL NL NOS CCAO SL 60 1.93233 6.15000 60 1.93250 6.18333 60 1.93317 6.25000 HS 7.68333 7.73333 7.65000 SE(N= 60) 0.818859E-03 0.626108E-01 0.645914E-01 5%LSD 172DF 0.228554E-02 0.174755 0.180283 - ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN5 2/ 9/** 20: PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CCAO 180 1.9327 0.28533 0.63429E-02 0.3 0.0000 0.7528 SL 180 6.1944 1.2377 0.48498 7.8 0.0000 0.5223 HS 180 7.6889 0.93527 0.50032 6.5 0.0000 0.6623 Th nghi m nh h ng c I ến s r r |NL | y (s u tu n theo i BALANCED ANOVA FOR VARIATE CD FILE TN6 23/ 8/** 21:58 PAGE thi nghiem VARIATE V003 CD 57 LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 3.11360 622721 444.00 0.000 NL 168778E-02 843890E-03 0.60 0.554 * RESIDUAL 172 241233 140252E-02 * TOTAL (CORRECTED) 179 3.35652 187515E-01 BALANCED ANOVA FOR VARIATE SR FILE TN6 23/ 8/** 21:58 PAGE thi nghiem VARIATE V004 SR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 454.133 90.8266 375.23 0.000 NL 433334 216667 0.90 0.413 * RESIDUAL 172 41.6335 242055 * TOTAL (CORRECTED) 179 496.200 2.77207 - TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN6 23/ 8/** 21:58 PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CD 0.257667 0.461333 0.530667 0.555000 0.671000 0.383000 SR 3.33333 4.06667 4.20000 4.50000 7.76667 6.73333 SE(N= 30) 0.683744E-02 0.898249E-01 5%LSD 172DF 0.190842E-01 0.250713 MEANS FOR EFFECT NL NL NOS CD 60 0.479167 60 0.472167 60 0.478000 SR 5.16667 5.08333 5.05000 SE(N= 60) 0.483480E-02 0.635158E-01 5%LSD 172DF 0.134945E-01 0.177281 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN6 23/ 8/** 21:58 PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | |NL | 58 NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CD 180 0.47644 0.13694 0.37450E-01 7.9 0.0000 0.5544 SR 180 5.1000 1.6650 0.49199 9.6 0.0000 0.4131 Th nghi m nh h ng c I ến s r r y (s u tu n theo i BALANCED ANOVA FOR VARIATE CD FILE TN7 24/ 8/** 18:26 PAGE thi nghiem VARIATE V003 CD LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 2.86065 572130 350.49 0.000 NL 298778E-02 149389E-02 0.92 0.405 * RESIDUAL 172 280770 163238E-02 * TOTAL (CORRECTED) 179 3.14441 175665E-01 BALANCED ANOVA FOR VARIATE SR FILE TN7 24/ 8/** 18:26 PAGE thi nghiem VARIATE V004 SR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 505.628 101.126 518.35 0.000 NL 877779 438889 2.25 0.106 * RESIDUAL 172 33.5555 195090 * TOTAL (CORRECTED) 179 540.061 3.01710 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN7 24/ 8/** 18:26 PAGE thi nghiem MEANS FOR EFFECT CT CT NOS 30 30 30 30 30 30 CD 0.312333 0.719667 0.513667 0.470667 0.444000 0.394000 SR 1.86667 6.46667 6.20000 4.76667 4.53333 2.73333 SE(N= 30) 0.737650E-02 0.806412E-01 5%LSD 172DF 0.205887E-01 0.225080 MEANS FOR EFFECT NL NL NOS CD 60 0.478000 SR 4.33333 59 60 60 0.470000 0.479167 4.50000 4.45000 SE(N= 60) 0.521597E-02 0.570220E-01 5%LSD 172DF 0.145584E-01 0.159155 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN7 24/ 8/** 18:26 PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CD 180 0.47572 0.13254 0.40403E-01 8.5 0.0000 0.4048 SR 180 4.4278 1.7370 0.44169 10.0 0.0000 0.1062 Th nghi m nh h ng c |NL | th n ho t t nh ến s r c y (s u tu n theo i BALANCED ANOVA FOR VARIATE CD FILE TN8 24/ 8/** 18: PAGE thi nghiem VARIATE V003 CD LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 17.2876 3.45753 ****** 0.000 NL 291000E-02 145500E-02 1.06 0.348 * RESIDUAL 172 235145 136712E-02 * TOTAL (CORRECTED) 179 17.5257 979088E-01 BALANCED ANOVA FOR VARIATE SR FILE TN8 24/ 8/** 18: PAGE thi nghiem VARIATE V004 SR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT 305.961 61.1923 439.97 0.000 NL 177778 888889E-01 0.64 0.534 * RESIDUAL 172 23.9221 139082 * TOTAL (CORRECTED) 179 330.061 1.84392 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE TN8 24/ 8/** 18: PAGE thi nghiem MEANS FOR EFFECT CT - 60 CT NOS 30 30 30 30 30 30 CD 0.422667 0.530667 0.700333 0.741333 0.892000 1.38500 SR 5.83333 6.06667 6.86667 7.03333 7.13333 9.83333 SE(N= 30) 0.675060E-02 0.680886E-01 5%LSD 172DF 0.188418E-01 0.190044 MEANS FOR EFFECT NL NL NOS CD 60 0.774667 60 0.777167 60 0.784167 SR 7.08333 7.15000 7.15000 SE(N= 60) 0.477340E-02 0.481459E-01 5%LSD 172DF 0.133231E-01 0.134381 ANALYSIS OF VARIANCE SUMMARY TABLE FILE TN8 24/ 8/** 18: PAGE thi nghiem F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT (N= 180) SD/MEAN | | | NO BASED ON BASED ON % | | | OBS TOTAL SS RESID SS | | | CD 180 0.77867 0.31290 0.36975E-01 4.7 0.0000 0.3483 SR 180 7.1278 1.3579 0.37294 5.2 0.0000 0.5341 |NL | 61