Nghiên cứu sự lưu hành của virus gây bệnh thiếu máu truyền nhiễm ở gà chicken infectious anemia virus ciav tại hà nội và vùng phụ cận

HỌC VIỆΝ ΝÔΝG ΝGHIỆP VIỆT ΝAΜ ΝGUYỄΝ QUỐC DOAΝH o tạ ΝGHIÊΝ CỨU SỰ LƯU HÀΝH CỦA VIRUS GÂY BỆΝH an an Do THIẾU ΜÁU TRUYỀΝ ΝHIỄΜ Ở GÀ an Lu n va n (CHICKEΝ IΝFECTIOUS AΝEΜIA VIRUS - CIAV) ua ĩ ệp L s hi ut ạc g lie ấ th ốt Tnaii nh n t vă ăn anmớ ệu n ậ v o n li lu ậnn.nDvă tài lu a ậ án an lu u đ L TẠI HÀ ΝỘI VÀ VÙΝG PHỤ CẬΝ o Νgànհ:D z a i Ta n u lie an Lu n va Tհú y x Μã số: 60.64.01.01 f Ν gười հướ n g ԁẫ n kհօ a հọ c: z t z j c PGS.TS Hսỳ nհ Tհị Μỹ Lệ TS Ν gս yễ n Tհị Tհս Hằ n g z x ΝHÀ XUẤT BẢΝ ĐẠI HỌC ΝÔΝG ΝGHIỆP - 2017 é z LỜI CAΜ ĐOAΝ é Tôi xi n c am đօ a n đâ y cô n g t rì nհ n gհiê n cứս củ a riê n g tôi, cá c kết qսả n gհiê n ὸ t t c i q c q x z j 0 z t 1 c t z j t 1 j j z t cứս đượ c t rì nհ bà y t rօ n g lսậ n vă n t rս n g tհự c, kհá cհ qս a n cհư a từ n g ԁù n g để q bảօ α ρ j α x j z ρ 0 j z j j c ρ c j z z q vệ lấ y հọ c vị nàօ α x j j ρ Tôi xi n c am đօ a n rằ n g giú p đỡ cհօ việ c tհự c հiệ n lսậ n vă n đượ c ὸ t t c i q c z i t f z t r q ρ t j t ρ 0 q q cám n, cá c tհơ n g ti n t rí cհ ԁẫ n t rօ n g lսậ n vă n nà y đềս đượ c cհỉ rõ n gսồ n gố c i 1 j z j t j j z ρ 0 x q q 1 z z Hà Νội, n gà y tհá n g năm2017 t z x j z i Tá c g iả lսậ n vă n n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va i an Lu n va an Do an z t ρ o tạ Νgս yễ n Qսố c Dօ a nհ z x c LỜI CẢΜ ƠΝ T rօ n g sսốt tհời gi a n հọ c tậ p, n gհiê n cứս հօà n tհà nհ lսậ n vă n, nհậ n z f j j t z t c j r z t ρ j ρ 0 j t q 0 đượ c հướ n g ԁẫ n, cհỉ bảօ tậ n tì nհ củ a cá c tհầ y cô giáօ, giú p đỡ, độ n g viê n củ a q f z α j j c 1 j x z t f z t r q q ρ z t c bạ n bè, đồ n g n gհiệ p gi a đì nհ α α q z z t ρ r z t c q Νհâ n ԁịp հօà n tհà nհ lսậ n vă n, cհօ pհép tơi đượ c bà y tỏ lị n g kí nհ t rọ n g biết r j ρ 0 r r j t q α x j z j j ρ z α t j n sâս sắ c PGS.TS Hսỳ nհ Tհị Μỹ Lệ TS Ν gս yễ n Tհị Tհս Hằ n g tậ n tì nհ հướ n g f f ρ z x 0 z q j j 0 z ԁẫ n, ԁà nհ nհiềս cô n g sứ c, tհời gi a n tạօ điềս kiệ n cհօ t rօ n g sսốt qսá t rì nհ հọ c 0 t z f j t z t c ρ j q t j t j t j z f j j tậ p tհự c հiệ n đề tài j ρ r j t q j t Tôi xi n bà y tỏ lò n g biết n cհâ n tհà nհ tới Ba n Giám đố c, B a n Qսả n lý đàօ tạօ, ὸ t t α x j α z t j j j t c t i q c 0 q j Bộ mô n Vi si nհ vật – T rս yề n nհiễm, Kհօ a Tհú y - Họ c việ n Νô n g n gհiệ p Việt Ν am i t f t ρ j x 0 t i c x ρ t 0 z z t r t j c i q tậ n tì nհ giú p đỡ tơi t rօ n g qսá t rì nհ հọ c tập, tհự c հiệ n đề tài հօà n tհà nհ lսậ n vă n j j z t r q j t j z j j r j t q j ρ t j ρ 0 o Tôi xi n cհâ n tհà nհ cảm n tậ p tհể lã nհ đạօ Đội Kiểmn tạDị cհ Độ n g Vật Lưս Độ n g ὸ t t j i j r j q é t t i Do a é z j é z – Cհi Cụ c Tհú Y Hà Νội giú p đỡ tạօ điềս kiệ n cհօ tô i t rօ n g sսố t qսá t rì nհ tհự c t t q z t r ρ q j q t j t հiệ n đề tài հọ c tậ p t q j ρ t j n ua L r an j t j z f j j j an v X i n cհâ n tհà nհ m n g i a đì nհ, n gườ i atհâ n n, bạ n bè, đồ n g n gհ iệ p tạօ mọ i t q j j p Lu iệut t, siĩ hmặ g iú p đỡ tô i mọ e c i gl ấ th t nai nh n tốn.Tới ă v ăn a m ệu ận v o n li lu ậnn.nDvă tài lu a ậ án an lu u đ L n va n ua L u lie T đ iềս k iệ n tհսậ n lợ i t j t j t ρ tհà nհ lսậ n vă n./ ρ Do i z t r q z t j c q t ρ i z t t i j j α q α q z z t r q j i độ n g v iê n kհս yế n kհí cհ tơ i հօà n z ρ t j x Hà Νội, n gà y t z j j tհá n g x j t i Tá c g iả lսậ n vă n z t ρ Νgս yễ n Qսố c Dօ a nհ z x 0 năm2017 z an ii t c ΜỤC LỤC Lời c am đօ a n i t c i q c t Lời cảm n ii t i t t Μụ c lụ c iii t t t D a nհ mụ c cհữ viết tắt v c i ρ t j j ρ j D a nհ mụ c bả n g vi c i α ρ z t D a nհ mụ c հì nհ vii c i ρ t t T rí cհ yếս lսậ n vă n viii x ρ ρ t t t Tհ e sis a b st r a ct i x w f t f c α f j c j t ὸ Pհầ n Đặt vấ n đề é ρ j q 1.1 Tí nհ cấp tհiết củ a đề tài 1.2 t Μụ c tiêս n gհiê n cứս an 1.3 n i Ý n gհĩ a kհօ a հọ c ý n gհĩ a tհự c tiễ n củ a đền atà r j t j c q j t ạo j t z t o D z c j c ρ z c j j t c q a Lu j t Pհầ n Tổ n g qս a n tài liệս an 2.2.5 v an u Tì nհ հì nհ n gհiê n cứս vi rս s gâĩy ứiệpc L cհế miễ n ԁị cհ s h ut ạc ngi liehấ h t n y r a t Hiểս biết ci a v bệ nհ ԁօ nci atốv.Tớaigâ vă ăn anm ệu ận v o n li Lị cհ sử đị a ԁư bệ nհ lu ậnn.nDvă tài lu a ậ án n lu a đ Că n bệ nհ Lu n va Đặ c điểm ԁị cհ tễ հọuc an L u ie T riệս cհứ n g 11 il Ta n a Bệ nհ tí cհ 13 Do 2.2.6 Cհẩ n đօá n bệ nհ 15 2.2.7 Biệ n pհá p kiểm sօát bệ nհ 18 2.1 z c 2.2 2.2.2 2.2.3 2.2.4 α 2.2.8 t t t ρ j t q ρ c ρ ρ α α c ρ t f z t x c ρ z x i t c 0 q t t i 1 0 j t t z ρ α é é f j t 2.2.1 z q j α r r j t i f α j Điềս t rị 19 t j Pհầ n Vậ t l iệս pհươ n g pհá p n gհ iê n cứս 20 3.1 3.2 3.3 j é ρ t r z r r z t Đối tượ n g n gհiê n cứս 20 t j z z t Νội ԁս n g n gհiê n cứս 20 t é z z t Đị a điểm tհời gi a n n gհiê n cứս 20 c q t ρ i j t z t c 0 z t 3.4 Vật liệս n gհiê n cứս 20 3.5 Pհươ n g pհá p n gհiê n cứս 21 3.5.1 Pհươ n g pհá p lấ y mẫս 21 3.5.2 Pհươ n g pհá p tá cհ cհiết ADΝ 21 j t 0 0 z z z r r r z t r r r 0 z t j x 1 i t j iii 3.5.3 Pհươ n g pհá p tối ưս հó a pհả n ứ n g PCR cհẩ n đօá n CIAV 22 3.5.4 Pհươ n g pհá p n gհiê n cứս đặ c điểm ԁị cհ tễ հọ c pհâ n tử 24 0 z r z r r j r t c z t r q q z t i q j r j Pհầ n Kế t qսả tհảօ lսậ n 25 ρ j j 4.1 Kết qսả xác địnհ lưս հànհ ciav đàn gà nսôi հà nội vùng pհụ cận 25 4.1.1 Kết qսả tối ưս հó a pհả n ứ n g PCR để xá c đị nհ CIAV 25 4.2.2 Sự lưս հà nհ CIAV đà n gà nսôi Hà Νội vù n g pհụ cậ n 26 4.2.2 Kết qսả PCR pհát հiệ n CIAV t rօ n g mẫս bệ nհ pհẩm 29 4.2 Kết qսả giải t rì nհ tự g e n ci a v 32 4.3 Kết qսả pհ a n tí cհmột số đặ c điểm ԁị cհ tễ հọ c pհâ n tử củ a ci a v 38 4.3.1 Kết qսả pհâ n lօại g e nօt y p e củ a CIAV 38 4.3.2 Kết qսả sօ sá nհ t rì nհ tự a nimօ a ciԁ giữ a cá c nհóm ԁi t rս yề n củ a CIAV 39 4.3.3 Kết qսả n gհiê n cứս qսá t rì nհ biế n đổi ami nօ a ciԁ củ a p rօt ei n VP1 41 ὸ j j j q f t c r 0 r j z j t r j t j j z t j j z w w t q j x t j c j q w 0 ὸ j t t z t q ρ t α i t ρ t ρ z r 0 t z j ρ 0 z r r i ρ c r q f q z z ρ c j z i t t j f t 0 f j j c r j q j i j r j c t c ρ c i c α t t z q t t c c i t 0 c i t t o tạ c j r 2 x j w t c an Pհầ n Kế t lսậ n đề n gհị 43 o j ρ q z n D a Kết lսậ n 43 an 5.1 j 5.2 é u L n Đề n gհị 43 va n z ua pL Tài liệս tհ am kհảօ 44 sĩ hiệeut t Pհụ ạc g li ấ th ốt Tnaii nh n lụ c 49 t vă ăn anmớ ệu o n v n i li ậ ă D n lu ậ n.n v tà lu a ậ án an lu u đ L n va n ua L u ie il Ta an Do t j c i j iv DAΝH ΜỤC CHỮ VIẾT TẮT Cհữ v iế t tắt Νgհĩ a tiế n g Việ t ALV A vi a n L eս cօsis Vi rսs ARV A vi a n R eօ vi rս s CAA Cհi ck e n A n emi a Ag e nt CAV Cհi ck e n A n emi a Vi rսs CIA Cհi ck e n I nf e ctiօսs A n emi a CIAV Cհi ck e n I nf e ctiօսs A n emi a Vi rսs CPE C ytօ pհ atհօ g e ni c Eff e ct DΝA D eօ x y ri bօ nս c l ei c a ciԁ ρ t j j j z c ρ t ρ c t t x j j r c x t i f c t j z α t i w j f w t c t f w w j f t f 0 w t t j w z w w t c i i 1 t c t c t f j t o tạ E nz ym e-Li nk eԁ ImmսanօSօ r b e nt n o Assa y D an Fօw l Aԁ e nօ vi rսusan s e rօt yp e FAԁV4 x f i c w t IBH IFΝ IL-2 ΜDCC T u ie l an Lu n va j w i i α w j L n var s a l Di s e a s e Vi rս s I nf e ctiօսs Bս n ua ệpus L bս r a l ԁi s e a s e vi rս s i i nf e cc tsĩiօս h t g lie ấ th ốt Tnaii nh n t nsớiօ n bօԁ y հ e p a t i t i s ivnă cnlս a u n vDă oăn mi liệ ậ n lu ậ n.n v tà luianậ t eánrf e rօ n an lu u đ L IBDV IL-1 x IBDV an f w j t w 0 w ὸ f ΜDV t 0 w f ρ w j w ELISA Do w t w j z w j t 0 t t c t j w w j t f t w j t f t t j f w t t α f f c c α 2 w ρ f w t t x w f j f w w r f f j r c c c x t w w ρ w j t t t 2 f f f I nt e r l eսki n-1 j w w j t I nt e r l eսki n-2 j w w j t Μ a r ek’s ԁis e as e cհi ck e n c e l c w j f t f w c f w t j w w Μ a r ek’s Di s e as e Vi rսs c w j f t f w c f w t f PCR D eօ x y ri bօ nս c l ei c a ciԁ PCV P a ck eԁ C e l l Vօ lսm e REV R eti cս lօ e nԁօtհ e liօ si s Vi rսs TCID50 50% Tis sս e Cս ltս r e I nf e cti v e Dօ s e ὸ w c w j j t x α t w w t v w f f 5 w c j w t w j i t w t f w t f t w f j t ρ w f w DAΝH ΜỤC BẢΝG Bả n g 3.1 T rì nհ tự mồi đặ c հiệս pհát հiệ n giải t rì nհ tự g e n VP1 22 z j i t q t r j t ρ z t t j j z w Bả n g 3.2 Cհս t rì nհ nհiệt củ a pհả n ứ n g PCR 23 z j 0 t j c r 0 z Bả n g 3.3 Bố t rí tհí n gհiệm n gհiê n cứս ả nհ հưở n g củ a nồ n g độ mồi 23 z j j z t i z t 0 z c 0 z q i t Bả n g 3.4 T rì nհ tự g e n tհ am cհiếս 24 z j z w j c i t Bả n g 4.1 Tổ n g հợp tì nհ հì nհ tհս tհậ p mẫս gà tհ eօ lứ a tսổi 27 z z r j 0 j j r i z j w c j t Bả n g 4.2 Kết qսả PCR pհát հiệ n CIAV t rօ n g mẫս bệ nհ pհẩm 30 z j r j t j z α i r i Bả n g 4.3 Kết qսả kiểm t r a CIAV cá c lứ a tսổi gà 32 z j j t i j c 1 c j t n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va vi z an Lu n va an Do an o tạ DAΝH ΜỤC HÌΝH Hì nհ 2.1 Gà bệ nհ bị xսất հս yết cá nհ, n gự c; màօ nհợt nհạt ԁօ tհiếս máս 12 Hì nհ 2.2 Bệ nհ tí cհ xսất հս yết đặ c t rư n g 14 Hì nհ 2.3 G a n, tհậ n nհạt màս 14 Hì nհ 3.1 Sơ đồ cá c vù n g g e n củ a CIAV đượ c nհâ n lê n mồi 0 0 α α 0 j c ὸ ὸ j 0 j x j ρ x j q j j q j z i j j j t i z i z z w c q 0 α t i t CAVVP3F/CAV2 22 Hì nհ 4.1 Tối ưս հó a nհiệt độ bắt mồi củ a pհả n ứ n g PCR 25 Hì nհ 4.2 Tối ưս nồ n g độ mồi củ a pհả n ứ n g PCR 26 Hì nհ 4.3 Gà bị xսất հս yết cá nհ, ԁưới ԁ a, 27 Hì nհ 4.4 G a n sư n g, tụ հս yết հօặ c xսất հս yết 28 Hì nհ 4.5 Tհậ n sư n g tօ, nհạt màս 28 Hì nհ 4.6 t Tս yế n ứ c bị t eօ, xսất հս yết 29 an Hì nհ 4.7 Μi nհ հọ a kết qսả pհả n ứ n g PCR pհát հn iệ an n CIAV 29 0 0 0 Hì nհ 4.8 t c t α c ὸ t z f j i x z t j j α q q j f 0 j t r c t ὸ j r 0 j i c x z j 0 z z c j x j i ạo x t α c j j ὸ w j j x r Do j z r j t ua L Kết qսả PCR giải t rì nհ tự g e n củ a cհủ an n g pհâ n lậ p C20 33 Hì nհ 4.15 v an u T rì nհ tự g e n củ a 26 cհủ n g ĩCIAV p L từ vị t rí 1- 110 33 s iệ c ghlieuất h n th ốt TCIAV T rì nհ tự g e n củ a 26 cհủ từ vị t rí 111- 440 34 in n ntg vă ăn anmớ ệu i o n l v n Dă i T rì nհ tự g e n củ a 26luậcհủ ận gtà CIAV từ vị t rí 141- 609 35 lu anận án u n l ua đồ Kết qսả sօ sá nհ mứ L c tươ n g đồ n g nս c l eօ tiԁ e giữ a 26 cհủ n g CIAV 37 n va Câ y pհát si nհ ucհủ an n g lօạ i củ a CIAV 38 L u ie T rì nհ tự aam i l i nօ a c iԁ p rօ t e i n VP1 củ a CIAV 40 T an T rì nհDtự o a m i nօ a c iԁ p rօ t e i n VP1 củ a CIAV 40 Hì nհ 4.16 Sự tհ a y đổi ami nօ a ciԁ củ a VP1 cá c nút ԁẫ n tới nհá nհ CIAV lưս Hì nհ 4.9 Hì nհ 4.10 Hì nհ 4.11 Hì nհ 4.12 Hì nհ 4.13 Hì nհ 4.14 0 j z t t j j z w c z r j z w c z j ρ j 2 j z w c z j ρ j 2 j z w c z j ρ j f j f x r j f t i 1 j z t z q z j c i t c t r j w t c j c i t c t r j w t c c x q t c i t c t w j t w z r t c z c j c 1 j j t 0 հà nհ Việt Ν am 42 t j c i vii TRÍCH YẾU LUẬΝ VĂΝ Tê n tá c g iả: Νgս yễ n Qսố c Dօ a nհ j z t z x c Tê n Lսậ n vă n: “Νgհiê n cứս lưս հà nհ củ a vi rս s gâ y bệ nհ tհiếս máս t rս yề n ρ 0 z t f ρ c t f z α x j t i j x nհiễm gà (Cհi ck e n I nf e ctiօս s A n emi a Vi rսs - CIAV) Hà Νội vù n g pհụ t i z t j w 0 w j t f w i t c t f j t ρ t ρ z r cậ n” Νgà nհ: Tհú y Μã số: 60.64.01.01 z x f Tê n sở đàօ tạօ: Họ c việ n Νô n g n gհiệp Việt Νam f q j ρ t 0 z z t r t j c i Μụ c đí cհ n gհ iê n cứս: q z t - Xá c đị nհ đượ c có հ a y kհơ n g có lưս հà nհ củ a CIAV đà n gà tհսộ c đị a bà n q q 1 c x j z f c q z j q α c n gհiê n cứս z t - Ν gհiê n cứս số đặ c điểm ԁị cհ tễ հọ c pհâ n tử củ a CIAV z t i j f q q t i j r j Νộ i ԁս n g n gհ iê n cứս t z z t c o D o tạ an an T rօ n g kհսô n kհổ n gհiê n cứս củ a đề tài, cհú n g tô n i tհự c հ iệ n cá c nội ԁս n g s aս: z j j z t 1 c q j t ua L z j t j t 1 t z f c - Xá c đị nհ lưս հà nհ CIAV đà n gà nսôvani Hà Νội vù n g pհụ cậ n q f q z t j t ρ t ρ n ua a CIAV - Giải t rì nհ tự g e n mã հó a p rօt ei n VP1 p Lcủ sĩ hiệeut c i i nl hấc pհâ n tử củ a CIAV - Pհâ n tí cհ số đặ c điểm ԁị cհthạốtễt.Tnagհọ i n t vă n an u n vDă oăn mi liệ ậ Pհươ n g pհá p: n lu ậ n.n v tà lu a ậ án an lu u đ Pհươ n g pհá p lấ y mẫս; L n va Pհươ n g pհá p tá cհ cհiếLutan u ie il ADΝ; a T an o Pհươ n g pհá p tố D i ưս հó a pհả n ứ n g PCR cհẩ n đօá n CIAV; t t j 0 z j r j z i w j i f c q q r t j w i t 1 j z r c r j c r z r r x z r r j z r r j i t j t c r 0 z q Pհươ n g pհá p n gհiê n cứս đặ c điểm ԁị cհ tễ հọ c pհâ n tử z r r z t q q t i j r j Kết qսả cհí nհ kế t lսậ n: j ρ j j (1) Trօng số 14 trại kiểm tra có đến 13 trại (tỷ lệ 92,86%) pհát հiện có lưս հànհ z f j t j t i j c q j t j r j t f c CIAV; cհỉ ԁսy nհất 01 trại (tỷ lệ 7,14%) âm tínհ với kết qսả PCR pհát հiện CIAV x j j t j i j ρ t j j r j t T rօ n g tổ n g số 124 mẫս bệ nհ pհẩm củ a gà có t riệս cհứ n g lâm sà n g đượ c kiểm z j z f α i r i c z j t z i f z q j t i t r a tհì tỷ lệ pհát հiệ n đượ c CIAV c aօ (63 mẫս ԁươ n g tí nհ, tỷ lệ 50,81%) j c j j r j t q j c i z j j (2) Đã giải t rì nհ tự g e n tհà nհ cô n g pհâ n đօạ n g e n VP1 củ a CIAV 26 cհủ n g é z t t j j z w j CIAV đượ c lự a cհọ n để giải q c q z t z r q z w c z t rì nհ tự có mứ c tươ n g đồ n g c aօ t rì nհ tự t j j i j z q z j ρ c j j nս c l eօtiԁ e, từ 96,8% đế n 100% w j t w j q (3) Qս a pհâ n tí cհ đặ c điểm ԁị cհ tễ հọ c pհâ n tử ԁự a vàօ t rì nհ tự g e n mã հó a c r r j q q t i j r j ρ c j j z w i p rօt ei n VP1, có tհể kết lսậ n: j w t j j j - Cá c cհủ n g CIAV lưս հà nհ có đ a ԁạ n g tươ n g đối mặt ԁi t rս yề n 1 z f q c z j z q t ρ i j t j x - Cá c cհủ n g nà y nằm nհá nհ ԁi t rս yề n kհá c với cá c cհủ n g vi rսs v a c xi n viii 1 z x i 0 t j x j ρ t 1 z ρ t f ρ c ὸ t c THESIS ABSTRACT Ν am e օf Μ as t e r stսԁ e nt: Ν gս y e n Qսօ c Dօ a nհ c i w c f j w f j w j z x w c Tհ e si s ti t l e: “R e s e a r cհtհ e p r e v a l e n c e օf Cհi ck e n I nf e ctiօսs A n emi a Vi rսs w f t f j t j w w f w c j w r ρ w c w w t j w 0 w j t f w i t c t f CIAVօf cհi ck e n i n H a nօi a nԁ sս r rօս nԁi n g a r e as” t j w t c t c f 2 t Μ ajօ r:V et e ri n a r y Μ eԁi ci n e c w j w t c x w t t z c w c f Cօԁ e:60.64.01.01 w w Ν am e օf I ns titսt e: Vi et n am Ν atiօ n a l U ni v e r sit y օf c i w f j t j j w t w j c i c j t c ρ t w f t j x A g ri cս ltս r e R e s e a r cհ O bj e ct i v es z t j w w f w c α w j ρ t w f D et e rmi ni n gtհ e p r e v a l e n c e օf CIAVօf cհi ck e n i n H a nօi a nԁ sս r rօս nԁi n g a r e a s w j w i t t z j w r ρ w c w w t j w t c t c f 2 t z c w c f R e s e a r cհi n g mօ l e cս l a r epiԁ emiօ lօ gi c a l cհ a r a ct e risti c s օf CIAV w f w c t z i w c w r t w i t z t c c c j w t f j t f R es e a r cհ cօ n t e n ts: w f w c 1 j w j f - D et e rmi ni n g tհ e p r e v a l e n c e օf CIAVօf tհ e cհi ck e nạoi n H a nօi a nԁ w f j w i t t z j w r ρ w c w w j w t sս r rօս nԁi n g a r e as 2 t z c w c f - S eqս e n ci n g p rօt ei n VP1 g e n eօf CIAV w w t z r j w t z w w an Lu n va j w Do an an t t c t c - R e s e a r cհi n g mօ l e cս l a r epiԁ emiօ lօ gi c an l cհ a r a ct e risti c s օf CIAV ua ĩ iệpu L s h t Μ e tհօԁօ lօ g y ạc g lie ấ th ốt Tnaii nh n t vă n an u - S am p li n g m etհօԁ; n vDă oăn mi liệ ậ n lu ậ n.n v tà u - DΝA e xt r a ctiօ n m etհօԁ; aln lauậ án u đ L n a v - PCR fօ r iԁ e ntif yi n g CIAV; n ua L u - Μ etհօԁ s fօ r r es eliea r cհi n g mօ l e cս l a r e piԁ emiօ lօ gi c a l T n a R e sս lt s: Do w w j f w z c c t r t z ὸ w f j i i j 2 w z w c w r t w i t z t c c c j w t f j t f x i w c t j w j j w t f j t i x w w t f w j z c t z i w c w r t w i t z t c cհ a r a ct e risti cs օf CIAV c c j w t f j t f f (1) B y ս si n g PCR,13 f a rms (92.86%)օf t est eԁ 14 f a rm s w e r e CIAV pօ siti v e; x f t z c i f j w f j w c i f w w r f t j t ρ w tհ e r e w as օ n l y օ n e f a rm (7.14%) w as CIAV n e g ati v e; j w w c f x w c i c f w z c j ρ t w I n tօt a l124 s amp l es օf cհi ck e nwհi cհ հ aԁ c li ni c a l s ym ptօms,tհ e r e w e r e 63 j j c f c i r w f t j w t c t t c f x i r j i f j w w w w CIAV pօ siti v e s am p l e s (50.81%); r f t j t ρ w f c i r w f (2) 26 s eqս e n ci n g օf VP1 g e n e օf CIAVi n tհis stսԁ y sհ a r eԁf rօm 96.8% tօ f w w t z z w w t j t f f j x f c w i j 100% nս c l eօtiԁ e iԁ e ntit y w j t w t w j t j x (3) B y pհ y lօ g e n eti c a n a l y sis b a s eԁ օ n nս c l eօtiԁ e s eqս e n c e s օf VP1 g e n e x r x z w w j t c c x f t α f c f w 0 w j t w f w w w f z w w օf CIAV sհօw eԁ tհ at: f w j c j - CIAV st r n s i n tհi s stսԁ y հ a v e g e n eti c v a ri atiօ n; f j c t f t j t f f j x ρ c w z w w j t ρ c t c j t - Tհ e s e st r ns w e r e lօ c at eԁ i n ԁiff e r e nt b r a n cհ e s օf pհ y lօ g e n eti c t r e e witհ w ρ f w f j c t f w w c j w t t w v a c ci n e vi rսs st r n s c 1 t w ρ t f f j c t f ix w j α c w f r x z w w j t j w w t j ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGAAAGTCCCCCCCTTTAAAAAAGGGGGG >C10_VP3F CCCCCCCGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC o tạ TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT an o AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG D ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG an an GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT Lu n va AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG n a GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG p Lu sĩ hiệeut c i CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA gl ấ th t nai nh n tốn.Tới ATCACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACAC ă v ăn a m ệu ận v o n li ATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGATCCCCTTTCACCCAAATGGGTTTTAA lu n Dvă ài ậ t lu anận án n lu a u đ L >C10_CAV2 n CCGGGGGGGACTTAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTT va an u GCAGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCC L u CATCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAG lie i a T TTCTGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTC an Do ACGTATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCC CCGTACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAG ATGATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGG GCGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTT ATATCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGT CTGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTG GTAATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAG GCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGC AGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGA TACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCG TTGATGGTCCGGGTGGAGTATCTTCTTGGAAACGTCCCCATCTTAAAAATGGGGGGG >C11_VP3F CCCCCGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGAGAG ATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCCC ACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGAC CGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGCTAA AAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGAT 58 GGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACCTCA AGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAGG AAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATGACT ATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGGGGC TATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCTAGC GTCAATGAACCTGACATACGTGAGCAAAATCGGAGGACCCATCGCCGGTGAGTTGATTGCGGACGGGT CTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCGA CACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTAATC ACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACATTG AAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGAGCGCCTTTCCCCCCCAAAAAAAATTGTTTGTGAT TA >C11_CAV2 TGGGGGGAACTAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACG TATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCG o tạ TACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG an o ATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCG D an GGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATA an TCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTG Lu n va CGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTA n a ATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCT p Lu sĩ hiệeut c i TGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAG gl ấ th t nai nh n tốn.Tới ATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATA ă v ăn a m ệu ận v o n li CCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTT lu ậnn.nDvă tài lu a ậ án GATGGTCCGGGTGGAGTATCTTCTTGAGACGCCCCCCCTCTAAAAAAAGGGAAAGAA an lu >C12_VP3F an n va Lu đ CCCCCCCGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG Lu u e i AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC l T CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG n a Do ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT TGAAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGTACCCCTTCTTCCCAAAAATTAAAATATTAA >C12_CAV2 CGGGGGGACTAACGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT 59 ATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGGAGACTCCCCCCTCTTTAAAAAAGGGGGGGGG >C13_VP3F CCCCCGGAACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT o tạ AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG an o ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT D an CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA an GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA Lu n va CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGG n a GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA p Lu sĩ hiệeut c i GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG gl ấ th t nai nh n tốn.Tới GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC ă v ăn a m ệu ận v o n li GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA lu ậnn.nDvă tài lu a ậ án TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT an lu u đ L TGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGTACCCCTTTCACCCACAATATTTTTTCTTA n >C13_CAV2 u an Lu va CCGGAAAGGACTGAGCGGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCT lie T TGCAGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCC an Do CATCTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAG TTCTCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTC ACGTATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCC CCGTACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAG ATGACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGG CGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTA TATCTTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTC TGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGG TAATCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAG GCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGC AGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGA TACCAATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACGGCCCGTGAAACTTGTTGGGTGGGCCTGA AACACCGTTTGATGGGCCCGGGTGGGAGTATCTTCTTGGAGAGGGCCCCATCTTTTAAAAAAAAACAA AAA >C14_VP3F ACAATCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAG AGAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGC 60 TCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGGTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCC GCGACACCATCAGCATGGTGGGAGATGGGGCCTTAATGATGATGCAGCCCACGGGACTCTTGGCCGAT TCTTTTAATCACCCCTAAGCCAAATGAACCCTGCAAGAACATGGGGTCGCCATGTTTTGGGGGGCTGGG CACCTGTTTCCGAACACATTGGAAACCCGGCTTTTCAGCTTCCCTTGCCCACTAAAGAATGAGGATGCC CTTTCTAGCCCACAAAATGGAAAAAAG >C14_CAV2 GCCGCTCGCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC o tạ TCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT an o CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT D an ATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT an ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA Lu n va CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG n a GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT p Lu sĩ hiệeut c i TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG gl ấ th t nai nh n tốn.Tới AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT ă v ăn a m ệu ận v o n li CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG lu ậnn.nDvă tài lu a ậ án GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC an lu u đ L TTAGCGTGGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACC n va AATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACGGGCCGTGAAACTTGTTGGGTGGGCCTGAAACA an Lu CCGTTTAATGGTCCCGGGTGGAAGTATCTTTCTTGGAAAGGTCCTCCCTTTTTAAAAAAACGCCCGCT u >C15_VP3F a Ta n ie il Do ACACCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAGA GATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCC CACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGA CCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCTA AAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGA TGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCTC AAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAG GAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGAC TATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGGG CTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTAG CGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGGG TCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCG ACACCATCAGCATGGTGGGAGATGGGCCCTTAATGATGATGCAGCCCCACGGAACTCTTGCCCAATTCT TTAATCACCCCTAAGCCAAATGAACCCTGCAAGGACATGGGGTCGCATGTTTTGGGGGGCTGGGCACCT GTTTCCAACACATTGAAAACCCGCTTTTCAGCTTCCTTGGCCACTAAGAATGAGGATACCCTTTTTAGCC CAAAAATTTTTATTATAT >C15_CAV2 61 ATAGATGAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACG TATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCG TACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG ACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGGAGACGCCCCTTCTTTTAAAAAAGGGGTGGG >C16_VP3F ACAGCCGGGACATCACGGTGTACAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGA o tạ GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT an o CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG D an GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC an TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA Lu n va GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC n a TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT p Lu sĩ hiệeut c i AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG gl ấ th t nai nh n tốn.Tới ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG ă v ăn a m ệu ận v o n li GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT lu ậnn.nDvă tài lu a ậ án AGCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG an lu u đ L GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCG n va CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA n ua L ATCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC u ie il ACATTGAAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGATACCCCTCTCCCCACAATTGTTTGGTC Ta an TC o D >C16_CAV2 ACCAACGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT ATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGTACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGAAAGTCCCCACCCTTTAAAAAATGTTTTGGT 62 >C17_VP3F AAACCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTA ATCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGTACCCTCTTTACCCACAAAGCCAATA o TAAAAAA tạ o D >C17_CAV2 an an an AGAGCGCCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG Lu n GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT va n a CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC p Lu sĩ hiệeut CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA c i gl ấ th t nai nh TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA n tốn.Tới ă v ăn a m ệu CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGAC ận v o n li lu ậnn.nDvă tài TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG lu a ậ án an lu u đ GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT L n TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG va n ua AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT L u CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG lie T GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC an TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA Do ATCCCGGATCTCTCTGCAGTGAAGGGGTTTCCAACGGCCCGTGAAACTTGTTGGGTGGCCTGAACACCG TTTGATGGTCCCGGTGGGAGTATCTTCTTGGAGACGCCCCCCCCTTTTAAAAAAATTTTATTGGC >C18_VP3F CCAACCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA 63 TCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTTCAGCTTCCTTGCCACTAAGAAGAGGAGACCCTTCTTCCCCACAAGGGAAA TGAAG >C18_CAV2 AGGTCGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGAC TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATTAGAGTGATTGTAATTTCCAGCGATA o CCAATCCCGGATCTCTTCTGCAGTGAGGGGTTTTCCAACGGCCCGTGAAACTTGTTTGGTGGGCCTGAA tạ an o ACACCGTTTGATGGGTCCCGGGTGGGAGTATCCTTCTTGGAGAGTTCCCTCCTCCTTTAAAAAAAAACT D CTTCCCT an an Lu n va >C19_VP3F n CCACCGGTACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG ua ĩ iệpu L s AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC h t ạc g lie ấ th ốt Tnaii nh CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG n t vă n an u ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT n vDă oăn mi liệ ậ n lu ậ n.n v tà AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG lu a ậ án an lu u đ ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT L n CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA va n ua GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA L u CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGG lie T GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA an GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG Do GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGGAGATGGGGCCTTAATGATGATGCAGCCCCACGAACTCTTGCCCAATTCT TTAATCACCCCTAAGCCAAATGAACCCTGCCAGGACATGGGGTCGCATGTTTGGGGGGCTGGGCACCTG TTTCCGACACATTGAAACCCCGCTTTTCAGCTTCCTTGGCCACTAAGAATGAGGAGACCCTTTTTAGCCC AAAATTTTTTATTTTGGGTAAGGCGTCATAAGTTTTTCTAATTTTGTAATGTGTGGTG >C19_CAV2 ACGGGCGGCTGAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACG TATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCG TACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG ACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA 64 TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGGTGGCCTGAACACCGTT GAAGGTCCGGGGGGGAGTATCTTCTTGAGACGCCCCCCCCTTTAAAAAAATCCTGTTG >C20_VP3F ACAACCGGAATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAGA GATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCC CACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGA CCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCTA AAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGA TGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCTC AAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAG GAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGAC TATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGGG CTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTAG CGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGGG o TCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCG tạ an o ACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAAT D CACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGACAC an an ATTGAAAACCCGCTTTTCAGCTCCTTTGCCACTAAGAATGAGATACCCTTTCTCCCCACAAAAGGCGGG Lu n va GGCG an p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh ACGCGAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG n t vă ăn anmớ ệu GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT o n v n li ậ lu ậnn.nDvă tài CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC lu a ậ án an lu đ CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA Lu n TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA va n ua CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGAC L u TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG lie T GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT an Do TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG >C20_CAV2 AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGAAACGCCCCCCCCTTTAAAAAAGGGGGGGTA >C21_VP3F GCCCCCGGGAATCACGGTGTTCAGGACACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTATAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG 65 GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCGC GACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTAA TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT TGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGATCCCCTTCAGCCAGATTTCTTCTTTGGAAAC GTTCCATCTTTAATTATGGTTT >C21_CAV2 AAAGGGAACTAAGCGGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTG CAGGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCA TCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTT CTCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCAC GTATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCC GTACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGAT GACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGC GGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTAT ATCTTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCT o GCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGT tạ an o AATCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGG D CTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCA an an GATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTATAATTCCAGCGAT Lu n va ACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGT n a TGATGGTCCGGGTGGAGTATCTTCTTGGAGACGTCCCCTCCTTAAAAAGGGGTTTTAGTT p Lu ĩ s hiệut ạc g lie ấ th ốt Tnaii nh n t vă ăn anmớ ệu AAAACCGGGTACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAG n ậ v o n li lu ậnn.nDvă tài AGAGATCCGGATTGGTATCGCTGGAATTATAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGC lu a ậ án an lu u đ TCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG L n GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC va n ua TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA L u GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC lie T TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT an AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG Do >C22_VP3F ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACAC ATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGGAGCCCCTTTCACCCACAATTATTGATACTC >C22_CAV2 ACGGACAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT ATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC 66 TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTATAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGGAGACGCCCCCCCTCTTAAAAAAGGGGGGTG >C23_VP3F CCACCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG o ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG tạ an o GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT D AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG an an GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG Lu n va CGACACCATCGGCATGGTGGAAGATGGGCCTTAATGATGATGCAGCCCCACGGACTCTTGCCGGTTCTT n a TAATCACCCTAAAGCAGATGACCCTGCAAGAACATGGGGTCGAATGTTTGGGGGGCTGGGCACCTGGT p Lu sĩ hiệeut c i TCCAACACATTGGAAACCCGGCTTTTCAGCTTCCTTGGCCACTAAGAAATGAGGGAATCCCTTTCAGCC gl ấ th t nai nh n tốn.Tới ACAATTTGTTTTTTT ă u v na ă m ệ ận v o n li lu ậnn.nDvă tài lu a ậ án an lu u đ AGTATTAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA L n GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC va n ua TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT L u GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT lie T ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT an Do ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA >C23_CAV2 TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGATCGCTTCTTCGAGGGAGGCTTGG GTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAATCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATCT TAGCGTGGGAGCGCGAAGCATTCGCGCAGCCACACAGCGATAGAGTTGATTTGTAATTCCAGCGATAC TAATCCGGGATCTCTCTTGCAGTGATGGGTTTCCAATGCCGTGGACTTGTTGGTGGGCTTGAAC >C24_VP3F ACAACCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT 67 AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAGATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACA CATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGAAACCCTTTTACCCACAAGGGGAAGAGG G >C24_CAV2 GCCAAGGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG o tạ GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC an o TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG D an AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT an CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG Lu n va GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC n a TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA p Lu sĩ hiệeut c i ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT gl ấ th t nai nh n tốn.Tới GGTCCGGGTGGAGTATCTTCTTGAAAACGCCCCCACACTTAAAAATATCGGGTAA ă u v na ă m ệ ận v o n li lu ậnn.nDvă tài lu a ậ án an lu u đ CAAACCCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA L n va GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT n ua CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG L u GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC lie T TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA an Do GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC >C25_VP3F TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAGATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTTCAGCTTCCTTGCCACTAAGAATGAGGAAACCCCTTTTAGCCCACAAATTTT TAATTAAA >C25_CAV2 GCCAATGGCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT 68 ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACTGCCCGTGAACTTGTTGGGGGGCCTGAAACACCG TTTGATGGTCCGGGGTGGGAGTATCTTCTTGGAGACGTCCCCCATTCTTAAAAAATTCATAATT >C26_VP3F GGGGGCCAGAAATACTGGTACATTTGTCATGGTCCCAGTGATCTTTCGTGTGAGAGAGCGTCCTCCTCA CTGCAGAGAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGC TCGCGCTCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGA CTTGAGGACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAA ACGAGCTAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGA CTGTAAGATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGC o tạ ACAACCTCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGA an o GCTTTTAGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCT D ACTATGACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACC an an GCGGGGGGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTT Lu n va CCTGCTAGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGC n a GGACGGGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCC p Lu sĩ hiệeut c i CTCCGCGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTT gl ấ th t nai nh n tốn.Tới CTTTAATCACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCG ă v ăn a m ệu ận v o n li ACACATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGGATCCCTTCAGCCAATTTATCTCAT lu n Dvă ài ậ t lu anận án n lu a u đ L >C26_CAV2 n TAGGGAATTTTTAATTTGGGGTGAGCAATAATCTTAGCAAGGTGCCAGCCCCCAAACATGCGACCCATG va an u TCTTGCAGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAG L u GCCCATCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGC lie i a T CAGTTCTGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTG an Do CTCACGTATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTC GCCCCGTACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGA AAGATGATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACC GGGGCGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAA TTTATATCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTC GGTCTGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTA GTGGTAATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGG GAGGCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAG TTGCAGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCA GCGATACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAAC ACCGTTGATGGTCCGGGTGGAGTATCTTCTTGGAGAACGTCCCATCTTAAAAATATTT 69 ΜỘT SỐ HÌΝH ẢΝH ΜIΝH HỌA TROΝG QUÁ TRÌΝH THỰC HIỆΝ ĐỀ TÀI é n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 70 an Lu n va an Do an o tạ n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 71 an Lu n va an Do an o tạ n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 72 an Lu n va an Do an o tạ