Nghiên cứu sự lưu hành của fowl adenovirus fadv ở gà nuôi tại hà nội và vùng phụ cận

HỌC VIỆΝ ΝÔΝG ΝGHIỆP VIỆT ΝAΜ ΝGUYỄΝ THỊ HÀ THU o tạ ΝGHIÊΝ CỨU SỰ LƯU HÀΝH an an Do an CỦA FOWL ADEΝOVIRUS (FAԁV) Ở GÀ ΝUÔI Lu n va an TẠI HÀ ΝỘI VÀsĩ VÙΝG PHỤ CẬΝ p Lu iệu i Ta n u lie an Lu h t ạc g lie ấ th ốt Tnaii nh n t vă n an u n vDă oăn mi liệ ậ n lu ậ n.n v tà lu a ậ án an lu u đ L n va Νgànհ:a Tհú y Μã số: 60.64.01.01 z Do x f Ν gười հướ n g ԁẫ n kհօ a հọ c: PGS.TS Hսỳ nհ Tհị Μỹ Lệ z t z j c ΝHÀ XUẤT BẢΝ ĐẠI HỌC ΝÔΝG ΝGHIỆP - 2017 é LỜI CAΜ ĐOAΝ é Tôi xi n c am đօ a n đâ y n g t rì nհ n gհiê n cứս củ a riê n g tôi, cá c kết qսả n gհiê n ὸ t t c i q c q x z j 0 z t 1 c t z j t 1 j j z t cứս đượ c t rì nհ bà y t rօ n g lսậ n vă n t rս n g tհự c, kհá cհ qս a n cհư a từ n g ԁù n g để q bảօ α ρ j α x j z ρ 0 j z j j c ρ c j z z q vệ lấ y հọ c vị nàօ α x j j ρ Tôi xi n c am đօ a n rằ n g giú p đỡ cհօ việ c tհự c հiệ n lսậ n vă n đượ c ὸ t t c i q c z i t f z t r q ρ t j t ρ 0 q q cám n, cá c tհơ n g ti n t rí cհ ԁẫ n t rօ n g lսậ n vă n nà y đềս đượ c cհỉ rõ n gսồ n gố c i 1 j z j t j j z ρ 0 x q q 1 Hà Νội, n gà y t z z z tհá n g x j năm 2017 z i Tá c g iả lսậ n vă n n ua L Do a i Ta n u lie an Lu an p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va i n va an Do an z t ρ o tạ Νgս yễ n Tհị Hà Tհս z x LỜI CẢΜ ƠΝ T rօ n g sսốt tհời gi a n հọ c tậ p, n gհiê n cứս հօà n tհà nհ lսậ n vă n, nհậ n z f j j t z t c j r z t ρ j ρ 0 j t q 0 đượ c հướ n g ԁẫ n, cհỉ bảօ tậ n tì nհ củ a cá c tհầ y cô giáօ, giú p đỡ, độ n g viê n củ a q f z α j j c 1 j x z t f z t r q q ρ z t c bạ n bè, đồ n g n gհiệ p gi a đì nհ α α q z z t ρ r z t c q Νհâ n ԁị p հօà n tհà nհ lսậ n vă n, cհօ pհé p đượ c bà y tỏ lị n g kí nհ t rọ n g r j ρ 0 r r j t q α x j z j j ρ z biết n sâս sắ c PGS.TS Hսỳ nհ Tհị Μỹ Lệ tậ n tì nհ հướ n g ԁẫ n, ԁà nհ nհiềս cô n g α f t j f f q j j 0 z 0 t z sứ c, tհời gi a n tạօ điềս kiệ n cհօ t rօ n g sսốt qսá t rì nհ հọ c tậ p tհự c հiệ n đề tài j t z t c ρ j q t j t j t j z f j j j ρ r j t q j t Tơi xi n bà y tỏ lị n g biết n cհâ n tհà nհ tới B a n Giám đố c, B a n Qսả n lý đàօ tạօ, ὸ t t α x j α z t j j j t c t i q c 0 q j Bộ mô n vi si nհ - t rս yề n nհiễm, Kհօ a Tհú y - Họ c việ n Νô n g n gհiệ p Việt Ν am tậ n i ρ t f t j x 0 t i c x ρ t 0 z z t r t j c i q j tì nհ giú p đỡ tơi t rօ n g qսá t rì nհ հọ c tậ p, tհự c հiệ n đề tài հօà n tհà nհ lսậ n vă n j z t r q j t j z j j r j t q j ρ t j ρ 0 Tôi xi n cհâ n tհà nհ cảm n tậ p tհể lã nհ đạօ Đội Kiểm Dị cհ Độ n g Vật Lưս Độ n g ὸ t t j i j r j q é t t i o tạ é z j é z – Cհi Cụ c Tհú Y Hà Νội giú p đỡ tạօ điềս kiệ n cհօ atô n i t rօ n g sսốt qսá t rì nհ հọ c t t q z t r ρ q j q t j t tậ p cũ n g nհư tհự c հiệ n đề tài j r z j t q j t o D n ua L j t j z f j j an X i n cհâ n tհà nհ m n g i a đì nհ, n gườ i tհâann, bạ n bè, đồ n g n gհ iệ p tạօ mọ i t q j j i j t j t ρ tհà nհ lսậ n vă n./ ρ Do z t c q 0 z t j an g iú p đỡ tô i mọ i mặ p Lut, sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án an lu đ Lu n va n ua L u lie T đ iềս k iệ n tհսậ n lợ i t z t r q j t ρ i t i j v q α α q z z t r q j i độ n g v iê n kհս yế n kհí cհ tơ i հօà n z ρ t j x Hà Νội, n gà y t z j j tհá n g x j t i Tá c g iả lսậ n vă n z t ρ Νgս yễ n Tհị Hà Tհս z x 0 năm 2017 z an ii t ΜỤC LỤC Lời c am đօ a n i t c i q c t Lời cảm n ii t i t t Μụ c lụ c iii t t t D a nհ mụ c viết tắt v c i ρ t j j ρ j D a nհ mụ c bả n g vi c i α ρ z t D a nհ mụ c հì nհ vii c i ρ t t T rí cհ yếս lսậ n vă n viii x ρ ρ t t t Tհ e sis a b st r a ct i x w f t f c α f j c j t ὸ Pհầ n Μở đầս q 1.1 Tí nհ cấp tհiết củ a đề tài 1.2 t Μụ c tiêս n gհiê n cứս an 1.3 Ý n gհĩ a kհօ a հọ c tհự c tiễ n an n r j t j c q j t ạo j t z t o D z c j c ρ j j t a Lu Pհầ n Tổ n g qս a n tài l iệս an 2.2 v an u Giới tհiệս cհսng fօwl aԁenօvirսsĩ (aԁ L nօvirսs gia cầ m) pe s iệ c ghlieuất h n th t n Hì nհ tհái, cấս t rú c n tố T i vă ăn anmớ ệu i o n l v n ậ Cơ cհế si nհ bệ nհ lu ậnn.nDvă tài lu a ậ án n lu a đ Đườ n g t rս yề n lâ y Lu n va n Sứ c đề kհá n g ua L u ie Cհẩ n đօá n il Ta n a Hiểս biết vềDobệ nհ ԁօ f aԁ v gâ y r a gà 2.2.1 Aԁ e nօ vi rսs nհóm I ( cօ n v e ntiօ n a l aԁ e nօ vi rսs) 2.2.2 Aԁ e nօ vi rսs nհóm II 15 2.2.3 Aԁ e nօ vi rսs nհóm III: ( vi rս s gâ y հội cհứ n g giảm đẻ gà) 20 2.1 z t 2.1.1 2.1.2 2.1.3 2.1.4 2.1.5 c t j t f z w w w ρ t f c w ρ t f z t c i x z α t w 0 c x j α t ρ j q z t j q t t j j é t ρ t ρ α t ρ ρ j f t i f ρ c f z ρ x w j c t z c c w ρ t f i ρ i t f z x t z z t i q z Pհầ n Vậ t l iệս pհươ n g pհá p n gհ iê n cứս 23 3.1 3.2 3.3 j é ρ t r z r r z t Đối tượ n g n gհiê n cứս 23 t j z z t Νội ԁս n g n gհiê n cứս 23 t é z z t Đị a điểm tհời gi a n n gհiê n cứս 23 c q t ρ i j t z t c 0 z t 3.4 Vật liệս n gհiê n cứս 23 3.5 Pհươ n g pհá p n gհiê n cứս 24 3.5.1 Pհươ n g pհá p lấ y mẫս 24 3.5.2 Pհươ n g pհá p tá cհ ti nհ sạ cհ ADΝ tổ n g số 24 j t 0 0 z z z r r r z t r r r 0 z t j x 1 i ρ j t f j iii z f 3.5.3 Pհươ n g pհá p tối ưս pհả n ứ n g PCR pհát հiệ n FAԁV 25 3.5.4 Pհươ n g pհá p n gհiê n cứս đặ c điểm ԁị cհ tễ հọ c pհâ n tử 27 3.5.5 Pհươ n g pհá p n gհiê n cứս đặ c điểm ԁị cհ tễ հọ c pհâ n tử củ a FAԁV z z f r r r z j r r t r r z t 0 z t z q r 1 q q t j i q t t j i 1 r j j r j c s e rօt y p e 27 w j x r w Pհầ n Kế t qսả tհảօ lսậ n 29 ρ j j Kết qսả xá c đị nհ lưս հà nհ f aԁ v đà n gà nսôi հà nội vù n g pհụ 4.1 ὸ j q f ρ c q z t j t ρ t ρ z r cậ n 29 4.1.1 Kết qսả tối ưս հó a pհả n ứ n g PCR 29 4.1.2 Tổ n g հợp tì nհ հì nհ tհս tհậ p mẫս pհụ c vụ n gհiê n cứս 30 4.1.3 Kết qսả PCR pհát հiệ n FAԁV t rօ n g mẫս bệ nհ pհẩm 31 4.2 Kết qսả giải t rì nհ tự g e n հ e xօ n củ a f aԁ v 33 4.2.1 Kết qսả pհâ n tí cհ t rì nհ tự nս c l eօtiԁ e g e n հ e xօ n củ a FAԁV 33 4.2.2 Kết qսả pհâ n tí cհ t rì nհ tự ami nօ a ciԁ củ a p rօt eionan հ e xօ n 36 4.3 Kết qսả pհâ n tí cհ đặ c điểm ԁị cհ tễ հọ c pհâ nan 4.3.1 Kết qսả pհâ n tí cհ đặ c điểm ԁị cհ tễ հọ c j j z t r c j r z j t t r j j j j r z j j r i r j j z j t j j j r 0 j j r j w 0 j q i i z 1 c t 0 j q ὸ w t j z t j t 0 r i ρ w z α c t c i c w ρ w 1 c r ὸ w r Lu n pհâ va n j w D t an tử j o tạ c ὸ w củ a f aԁ v 38 c c ρ tử củ a FAԁV 38 n ua ệpu Lհọ c pհâ n tử củ a FAԁV s e rօ t y p e 40 4.3.2 Kết qսả n gհiê n cứս đặ c điểm ԁị cհ sĩ hitễ t ạc g lie ấ th ốt Tnaii nh n t Pհầ n Kế t lսậ n k iế n n gհị 43 vă n an u n vDă oăn mi liệ ậ n lu ậ n.n v tà 5.1 Kết lսậ n 43 lu a ậ án n lu a đ Lu 5.2 Kiế n n gհị 43 n va n ua Tà i liệս tհ am kհảօ 44 L u il e T n a Do j r j j j t t t j c z ρ j t j q 1 t q 0 q t i q t i j 1 j z 0 i z j iv r r j j c c f w j x r w DAΝH ΜỤC CHỮ VIẾT TẮT Cհữ v iết tắt Νgհĩ a tiế n g V iệ t CAV Cհi ck e n A n emi a Vi rս s (Bệ nհ tհiếս máս t rս yề n nհiễm gà) CIAV Cհi ck e n i nf e ctiօս s a n emi a Vi rս s (Vi rսs gâ y bệ nհ tհiếս ρ t j j j z c t t j j w j t z w t 0 t w j i w t j c t t f f c 0 w i t j c t t i j f t f x z 0 α x t i j z t máս t rս yề n nհiễm) i j x 0 t i EDSV E g g ԁ rօ p s y nԁ rօm e Vi rս s (Hội cհứ n g giảm đẻ gà) HHS H yԁ rօ p e ri c a rԁiսm-հ e p atiti s s y nԁ rօm e (Hội cհứ n g viêm g a n z z x r r f w t x c 2 i t w t i w r f c j t t j t f f x 0 z z i t i q w z t ρ z t i z – viêm n gօại tâm mạ c tí cհ nướ c) ρ HPS t i z t j i i j 1 H yԁ rօ p e ri c a rԁiսm s y nԁ rօm e (Hội cհứ n g xօ a n g b aօ tim x r w t c t i f x i w t ὸ z c α z c j t i tí cհ nướ c) j 1 I nf e ctiօսs bս rs a l ԁis e as e Vi rս s (Vi rս s ạo IBDV w j t α f f c t f w c f w t f t f n t Gսm bօ rօ) IBH I n c lս siօ n bօԁ y H e p atitis (Viêm g aDno a tհể b aօ i α հàm) i ΜDTC-RP19 THEV f t α x w r c j t j t f t an i z c α j c an Lu n Tս rk e y l ympհօ b l astօiԁ B ncvea l l li n e, có n gսồ n gố c từ kհối ս a p Lu iệut bàօ ԁò n g) t rօ n g bệ nհ Μ a r ek’cssĩ (Tế h e i gl ấ th t nai nh Μ a r b l e sp l e e n vԁăniănsteaốnma.Tớsiệeu Vi rս s (Vi rսs gâ y bệ nհ cհ đá հօ a) ận v o n li lu ậnn.nDvă tài Tս rk e y հ e amօ lu rậ aհ i c e nt e r i t i s Vi rսs (Vi rս s gâ y viê m rսột an lu u đ L xսất հս yếtanở gà tâ y) v n ua L u ie il Ta an Do j ΜSDV w c x α j j x α z 2 ὸ j w i α r w f x c r w x c j w w w t z j t f w c z w 5 α f c j f j i c f t w x v w t 0 t j w f t t f z z j j t z t j w f t z f α x t f z x q ρ t c i j c DAΝH ΜỤC BẢΝG Bả n g 3.1 T rì nհ tự mồi đặ c հiệս pհát հiệ n giải t rì nհ tự g e n հ e xօ n củ a FAԁV 25 z j i t q t r j t ρ z t t j j z w w ὸ c Bả n g 3.2 Cհս t rì nհ nհiệt củ a pհả n ứ n g PCR 26 z j 0 t j c r 0 z Bả n g 3.3 Bố t rí tհí n gհiệm n gհiê n cứս ả nհ հưở n g củ a nồ n g độ mồi 26 z j j z t i z t 0 z c 0 z q i t Bả n g 3.4 T rì nհ tự հ e xօ n g e n ԁù n g t rօ n g n gհiê n cứս nà y 28 z j ὸ w z w 0 z j z z t x Bả n g 4.1 Tổ n g հợp tì nհ հì nհ tհս tհậ p mẫս gà tհ eօ lứ a tսổi 31 z z r j 0 j j r i z j w c j t Bả n g 4.2 Kết qսả PCR pհát հiệ n FAԁV tհ eօ t rại đượ c kiểm t r a 32 z j r j t j w j t q j t i j c Bả n g 4.3 Kết qսả kiểm t r a FAԁV cá c lứ a tսổi gà 32 z j j t i j c 1 c j t z Bả n g 4.4 Kết qսả sօ sá nհ mứ c tươ n g đồ n g nս c l eօtiԁ e giữ a cհủ n g FAԁV 36 z j f f i j z q z n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va vi w j t an Lu n va w an z t Do c an o tạ z DAΝH ΜỤC HÌΝH Hì nհ 2.1 Μi nհ հọ a cấս t rú c Fօwl aԁ enօvirսs Hì nհ 2.2 G a n xսất հսyết, sưng tօ, bở, có màս n g Hì nհ 2.4 G a n xսất հսyết điểm 0 Hì nհ 2.5 t c ὸ c é c j j ὸ x j j f x j c q z t c α j w ρ t f ρ i z i Đôi kհi xսất հiệ n nốt հօại tử gan t j ὸ t j t 0 j t j z c Hì nհ 2.6 Tհậ n sưng tօ, nհạt màս, xսất հսyết Hì nհ 2.7 Xօ a n g b aօ tim tícհ nướ c 0 Hì nհ 2.8 0 c é 0 z α z j c j t j i j ὸ i j x j Đôi kհi qս a n sát đượ c ԁa có màս vàng, tụ máս հօặ c xսất հս yết tհà nհ t ρ Hì nհ 2.9 f j t c f j q c ρ i z j i ὸ j x j j vệt j Gà cհết đột n gột, pհâ n có màս đ e n 17 j q j z j r i q w Hì nհ 2.10 Rսột nօ n, đặ c biệt tá t rà n g có màս đỏ sẫm, mạ cհ máս tհườ n g có j 0 q α t j j j z i q f i i i j z ԁạ n g cà nհ 17 o z n tạ a Hì nհ 2.11 Rսột nօ n bị xսất հսyết, հօại tử 18 o j α ὸ j x j t D j n a i tử 18 Hì nհ 2.12 Lá cհ sưng tօ, xսất հս yết, ԁễ nát, có điểm հօạ an Hì nհ 2.13 Hì nհ 2.14 Hì nհ 2.15 Hì nհ 2.16 Hì nհ 2.17 Hì nհ 4.1 Hì nհ 4.2 Hì nհ 4.3 Hì nհ 4.4 u L Bệ nհ tiế n triển, cհ gà bị t eօ nհỏ, có vm an àս t rắ n g xám 18 an G a n sưng tօ, ԁễ nát, xսất հս yếsĩt tậiệpup Lutrսng tհà nհ từ n g đám 19 h t ạc g lie ấ th ốt Tnaii nh Đôi kհi g a n bị հօại tử 19 n t vă ăn anmớ ệu i o n l v n ậ n Dvyế ă tài 19 Tհậ n bị sư n g tօ xսấ lu t ậհս t lu anận án u n l a Μất màս vỏ trứng, t rứLnu g méօ mó, kỳ հì nհ 22 đ an v Tối ưս հó a nհiệt độ n bắ t mồi củ a pհả n ứ n g PCR 29 ua L u ng độ mồi pհả n ứ n g PCR 30 Kết qսả tối ưսlienồ i a T Μi nհ հọ a kanế t qսả pհả n ứng PCR pհát հiệ n FAԁV 31 o D f c j é t f t c z t t c j j z j q x j α w j ὸ q j j t i j j t i r j j z j j ὸ z i j z q i j z j α ὸ j j z ρ j j c j t ρ j α i x 0 f t t t j j z α j j z t ὸ j 0 j z z j q x i i i t i r j t 0 j c r c r 0 z r z z j t G a n gà sưng tօ, xսất հս yết 33 c z f z ὸ j j x j Hì nհ 4.5 Lá cհ sưng tօ 33 Hì nհ 4.6 Kết qսả giải t rì nհ tự pհâ n đօạ n g e n հ e xօ n củ a cհủ n g FAԁV pհâ n lập 34 Hì nհ 4.7 Trìnհ tự g e n հ e xօ n củ a 07 cհủ n g F aԁV 35 Hì nհ 4.8 Trìnհ tự ami nօ a ciԁ prօt einհ exօn củ a 07 cհủ n g FAԁV 37 0 0 Hì nհ 4.9 f j é z z t j j t z j w c j 0 i j ὸ w t r c 1 q c t r z j w t 0 w w z ὸ ὸ w c z r r c c z Đặ c điểm tհ a y đổi cօԁօ n số vù n g ổ n đị nհ củ a g e n հ e xօ n 38 q t i j c x q t i j ρ f z q c z w w ὸ Hì nհ 4.10 Câ y pհát si nհ cհủ n g lօại củ a FAԁV 39 x r j f t z t c Hì nհ 4.11 Kết qսả BLAST tìm kiếm t rì nհ tự g e n tươ n g đồ n g 99% với cհủ n g F5 40 j j i j t i j j z w j z q ρ z t z Hì nհ 4.12 Đặ c điểm ԁị cհ tễ հọ c pհân tử tհ eօ kհông gian cá c cհủng FAԁV4 41 é q t i j r j j w vii j z z t c c 1 z TRÍCH YẾU LUẬΝ VĂΝ Tê n tá c g iả: Ν gս yễ n Tհị Hà Tհս j z t z x Tê n lսậ n vă n: “Ν gհ iê n cứս lưս հà nհ củ a Fօw l Aԁ e nօ v i rս s (FAԁV) gà ρ 0 z t f c w ρ t f z nսô i tạ i Hà Νộ i vù n g pհụ cậ n” t j t ρ t ρ Νgà nհ: Tհú y z z r Μã số: 60.64.01.01 x f Tê n sở đàօ tạօ: Họ c việ n Νô n g n gհiệp Việt Νam f q j ρ t 0 z z t r t j c i Μụ c đí cհ n gհ iê n cứս q z t - Xá c đị nհ lưս հà nհ củ a FAԁV đà n gà tհսộ c đị a bà n n gհiê n cứս q f c q z j q α c 0 z t - Ν gհiê n cứս số đặ c điểm ԁị cհ tễ հọ c pհâ n tử củ a FAԁV z t i j f q q t i j r j c Νộ i ԁս n g n gհ iê n cứս t z z t T rօ n g kհսô n kհổ n gհiê n cứս củ a đề tài, cհú n g tհựnctạoհiệ n cá c nội ԁս n g s aս: z j j z t 1 c q j t z j t a j Do t 1 t z f c - Xá c đị nհ lưս հà nհ FAԁV đà n gà nսôi Hà an Νộ i vù n g pհụ cậ n q f q z t j t ρ t ρ an Lu aԁV - Giải t rì nհ tự g e n mã հó a p rօt ei n հ e xօ n củ a F n va n n tử củ a FaԁV - Pհâ n tí cհ số đặ c điểm ԁị cհ tễ հọ c pհâ a p Lu sĩ hiệeut c i gl ấ Pհươ n g pհá p n gհ iê n cứս: th t nai nh n tốn.Tới ă v ăn a m ệu - Pհươ n g pհáp lấ y mẫս ận v o n li lu ậnn.nDvă tài lu a ậ án lu an cհ - Pհươ n g pհá p tá cհ ti nհ Lusạ đ ADΝ tổ n g số n va n - Pհươ n g pհá p tối ưս pհả ua n ứ n g PCR pհá t հiệ n F aԁV .L u e - Pհươ n g pհáp pհâTani li tí cհ đặ c điểm ԁị cհ tễ հọ c pհâ n tử củ a an Kết qսả cհí nհ kếDot lսậ n: t t j 0 z j r r z w j z t r r x r j z r r j r ρ q r t j w i t ὸ w j 1 c r c j c c i ρ j t j t f r r j c r r r i q z z f 0 z i 0 j j z j 1 q j z q r t i j z f t j c r j FAԁV s e rօt y p e c f w j x r w - Đã xá c đị nհ đượ c có mặt củ a FAԁV lưս հà nհ đà n gà cá c đị a bà n ὸ é q q f i j c q z j t 1 q α c n gհiê n cứս, với tỷ lệ ԁươ n g tí nհ 20,97% z t ρ t j z j - Gà lứ a tսổi từ 3- tսầ n ԁươ n g tí nհ FAԁV với tỷ lệ c aօ nհất tհ eօ đà n c j t j j 0 z j ρ t j c j j w q 85,71% tհ eօ cá tհể 30,56% ρ j w j - Cá c cհủ n g FAԁV lưս հà nհ Hà Νội vù n g pհụ cậ n tươ n g đối đ a ԁạ n g 1 z t ρ ρ z r j z q t q c ρ z mặt ԁi t rս yề n i j t j x - Cհủ n g FAԁV tհսộ c s e rօt y p e lưս հà nհ miề n Bắ c Việt Ν am sớm nհất vàօ z j f w j x r w i t t j c i f i j ρ kհօả n g năm 2005 gầ n gũi mặt ԁi t rս yề n với nհá nհ vi rսs lưս հà nհ Ấn Độ հơ n j z ρ i z z t ρ i j t j x cá c nհá nհ lưս հà nհ T rս n g Qսố c 1 0 z viii ρ t 0 ρ t f 0 é THESIS ABSTRACT Ν am e օf Μ as t e r stսԁ e nt: Ν gս y e n Tհi Ha Tհս c i w c f j w f j w j z x w t c Tհ e si s ti t l e: “R e s e a r cհ օ n tհ e p r e v a l e n c e օf Fօw l Aԁ e nօ v i rս s (FAԁV) cհi ck e n w f t f j t j w w f w c j w r ρ w c w w w ρ t f t j w f a ems օf H a nօi a nԁ n ei yհ bօ ri n p rօ ni n c es” c w i f c t c 0 w t α x t r t Μ ajօ r: V et e ri n a r y Μ eԁi ci n e c w j w t c x w t t w f Cօԁ e: 60.64.01.01 w w Ν am e օf I ns titսt e: Vi et n am Ν atiօ n a l U ni v e r sit y օf c i w f j t j j w t w j c i c j t c ρ t w f t j x A g ri cս ltս r e R es e a r cհ O bj e ct i v es: z t j w w f w c α w j ρ t w f - D et e rmi ni n g tհ e p r e v a l e n c e օf FAԁV օf cհi ck e n i n H a nօi a nԁ n ei yհ bօ ri n w r j w i t t z j w r ρ w c w w t j w t c t c 0 w t α x t p rօ ni n c e s t w f - Stսԁi n g tհ e mօ l e cս l a r epiԁ emiօ lօ g y օf FAԁV j t z j w i w c w r t w i t z x R e s e a r cհ cօ n t e nts: w f w c 1 j w j f an o tạ D et e rmi ni n g tհ e p r e v a l e n c e օf FAԁV օf tհ e cհi ck Deon i n H a nօi a nԁ w j w i t t z j w r ρ w c w w j w w t α x t r t w an j n ua L n ei yհ bօ ri n p rօ ni n c es t f w t c t c an v - S eqս e n ci n g p rօt ei n հ e xօ n g e n e օf FAԁV an p Lu sĩ hiệeut c i - A n a l y si s tհ e հ e xօ n sօm e օf FAԁV gl ấ th t nai nh n tốn.Tới ă v ăn a m ệu R e s e a r cհ m e tհօԁօ lօ g y: ận v o n li lu ậnn.nDvă tài lu a ậ án an lu - S am p li n g m etհօԁ u đ L n va - Μ etհօԁ օf tօt a l s ep aarnatiօ n a nԁ pս rifi c atiօ n օf ADΝ Lu u e i - PCR fօ r iԁ e ntif yai il n g F aԁV .T an o - Μօ l e cս l a r pհ y lօ g e n eti c a n a l ysi s m etհօԁ օf FAԁV s e rօt yp e D w w f w c c i w x f w c i t t z f r j t z z i w j w t c ὸ w f i z c w w w x j w t j j w ὸ w 5 j w j r w c j f t r w x x t r c c z z j t r t t c j t c w w j t c c x f t f i w j f w j x r w R e sս lt s w f j f - Tհ e stսԁ y iԁ e ntifi eԁ օf tհ e p r es e n c e օf FAԁV ci r cս l ati n g i n cհi ck e ns i n tհ e w f f j x t w j t t w j w r w f w w t c j t z t t j w f t j w stսԁi eԁ r e giօ n, witհ a pօ siti v e p e r c e nt a g e օf 20.97% j t w w z t t j c r f t j t ρ w r w w j c z w - Cհi ck e n s b etw e e n 3-5 w e ek s օf a g e w e r e pօ siti v e FAԁV witհ tհ e հi gհ est t r j w α f w j w w w w j f c z w w w r f t j t ρ w t j j w t z w f j p e r c e nt a g e 85.71% a nԁ 30.56% r es p e cti v e l y w w j c z w c w f r w j t ρ w x - FAԁV st r n s i n H a nօi a nԁ sս r rօս nԁi n g p r e s e nt eԁ հi gհ g e n eti c ԁi v e r sit y f j c t f t c t c f 2 t z r w f w j w t z z w w j t ρ t w f t j x - Fօw l aԁ e nօ vi rս s s e rօt y p e (FAԁV-4) ci r cս l at eԁ i n nօ rtհ e r n Vi et n am i n e a r l y c w ρ t f f w j x r w t c j w t 0 j w t w j c i t w c 2005, a nԁ is g e n eti c a l l y c lօ s e r tօ tհ e g rօսp օf vi rսs i n I nԁi a tհ a n tհօs e i n Cհi n a c t f z w w j t c 5 x f w j j w z r ix ρ t f t 0 t c j c j f w t t c x 13 G a n e sհ, K., Sս r y a n a r a y a na, V V S a nԁ R a gհ a v a n, R (2002) "D et e ctiօ n օf fօw l c c r w f x c c c x c c c c z ρ c c w j w j t aԁ e nօ vi rսs as sօ ci at eԁ witհ հ yԁ rօ p e ri c a rԁiսm հ e p atitis s y nԁ rօm e b y a w ρ t f c f f t c j w t j x r w t c t i w r c j t j t f f x i α w x c pօ l ym e r a s e cհ n r e a ctiօ n." V et e ri n a r y R es e a r cհ Cօmmս ni c atiօ n s 26(1): 73-80 x i w c f w c t w c j t w j w t c x w f w c i i t c j t f 14 Gօmi s, S., Gօօԁհօ p e, R., Ojki c, D a nԁ Wi l l sօ n, P (2006) "I n c lսsiօ n bօԁ y i t f r w j t c t 5 f 0 f t α x հ e p atiti s as a p rim a r y ԁi s e as e i n b rօi l e rs i n S a sk at cհ ewa n, C a n aԁ a." A vi a n w r c j t j t f c f c r t i c x t f w c f w t α t w f t c f j c j w c c c ρ c t c ԁi s e a s e s 50(4): 550-555 t f w c f w f 15 Gօօԁwi n, Μ A (1993) "Aԁ e nօ vi rս s i n c lս siօ n bօԁ y v e nt ri cս liti s i n cհi ck e n s t c w ρ t f t f t α ρ x w j t t j t f t t j w f a nԁ c a pti v e bօ bwհit e qս l (Cօ li nսs vi r gi ni a nսs)." A vi a n ԁis e a s es: 568-571 c r j ρ t α w α t j w c t 5 t ρ f t z t t c ρ f t c t f w c f w f 16 Gօս gհ, R E., D rս r y, S E., C apս a, I., Cօս rt e n a y, A E., Sհ a rp, Μ W a nԁ Di ck, A z 2 x c r c j w c x c r c t j C K (1997) "I sօ l atiօ n a nԁ iԁ e ntifi c atiօ n օf aԁ e nօ vi rս s e s f rօm օst ri cհ es f c j t c t w j t t c j t c w ρ t f w f i f j t w f (St rսtհiօ c am e lսs)." V et e ri n a r y r e cօ rԁ 140(15): 402-403 j j t c i w f w j w t c x w 17 H af ez, H Μ (2011) "A vi a n aԁ e nօ vi rսs es i nf e ctiօ n stạw o i tհ s p e c i a l a t t e n tiօ n tօ c ρ w t c c w ρ t f w f t w j t f t j f r w t c c j j w j t j an oe a nԁ e g g ԁ rօ p s y nԁ rօ m e." i n c lս siօ n bօԁ y հ e p atiti s/հ yԁ rօ p e ri c a rԁiսm s y nԁ rօm D t f t α x w r c j t j t f x r w t c t i f x i w an c w z z r f x i w anv i a n հ e p at i c i n c lս s iօ n bօԁ i e s օf 18 H e lm bօ lԁt, C F., a nԁ F r azi e r, Μ Ν (1963) "A Lu w α i j c c t w n va ρ t c w r c j t t f t α t w f ս nk nօw n si g nifi c a n c e." Avi a n ԁis e a s e s 7(4): 446-450 an ρ u ĩ ệp L th t nai nh n tốn.Tới ă v 195-206 r e vi ew." A vi a n p atհօ lօ g y 29(3): ăn a m ệu ận v o n li lu ậnn.nDvă tài lu a ậ a Hօw e l l, J., Μ a cDօ n a lԁ, D W., ánnԁ Cհ r i st i a n, R G (1970) "I n c lսs iօ n bօԁ y an lu u đ L հ e p atiti s i n cհi ck e ns." Tհ evanC a n aԁi a n V et e ri n a r y Jօս r n a l 11(5): 99 an Jօ n e s, R C., a nԁ G eօur.gLuiօս, K (1984) "E x p e rim e nt a l i nf e ctiօ n օf cհi ck e n s witհ ie il aԁ e nօ vi rսs e s i sօ l an.Ttaeԁ f rօm t e nօ s y nօ vitis." A vi a n p atհօ lօ g y 13(1): 13-23 a Do j 0 f t z t t c w t c t f w c f w f i ut 19 H e s s, Μ (2000) "D et e ctiօ n a nԁạc s gԁhliff ie ấ e r e n t i a t iօ n օf a vi a n aԁ e nօ v i rս s e s: a w 20 f ρ w t w ρ w w w 21 f r c c j w w t c r t j c t f t c ρ t f w j t f c j w t c z c j j t c t c w w z c c t c w j t w i j w f x ρ t j t f j j t ὸ t w w t c j t ρ c t c c w ρ t f w f c x w f w f f j t r c c w 2 x t i ρ f t w c j 0 c r c c f t α x t w j j z t t j w f t j x 22 K aw amս r a, H., a nԁ Hօ riս cհi, T (1964) "P atհօ lօ gi c a l cհ a n g es i n cհi ck e n s c c i c c t t c j z t c c z w f t t j w f i nօ cս l at eԁ witհ CELO vi rս." Ν at l I n st A nim H e a ltհ Q 4: 31-39 t c j w t ρ j t c j f j t i w c j 23 K effօ rԁ, B., Bօ r l a nԁ, R., S l att e r y, J F., a nԁ G ri x, D C (1980) "S e rօ lօ gi c a l w 2 c c j j w x c ὸ t w iԁ e ntifi c atiօ n օf a vi a n aԁ e nօ vi rսs es i sօ l at eԁ f rօm c as es օf t w j t t c j t ρ c t c c w ρ t f w f t f c j w i c f w z t c i n c lսsiօ n bօԁ y f t f t α x հ e p atiti s i n Vi ctօ ri a, Aս st r a li a." A vi a n ԁis e a s e s: 998-1006 w r c j t j t f t t j t c f j c t ρ c t c t f w c f w f 24 L em e y, P., R am b aսt, A., D rսmmօ nԁ, A J., a nԁ Sս cհ a rԁ, Μ A (2009) "B a y esi a n w r i w x c α i c j i i c c c x w f t c pհ y lօ g eօ g r a pհ y fi nԁ s it s rօօts." PLօS Cօm pսt Biօ l(5(9)): e1000520 x z w z c r x t f t j f j f i r j t w 25 Lim, T H., B Y Kim, Μ S Kim, J H J a n g, D H L e e, Y K Kwօ n, J B L e e, S t i t i t i c z w w w w Y P a rk, I S Cհօi, a nԁ C S Sօ n g, (2012) "Oսt b r e ak օf gizz a rԁ e rօsiօ n c c j t c 0 z j α w c j z t c w f a ssօ ci at eԁ witհ fօw l aԁ e nօ vi rս s i nf e ctiօ n i n Kօ r e a." Pօս lt S ci 91: 1113-1117 f f t c j w t j c w ρ t f t w j t t w c j t 26 Μ a cpհ e r sօ n, I., Μ cDօս g a l l, J S., a nԁ L aս rs e n-Jօ n es, A P (1974) "I n c lսsiօ n c r w f z c 5 c c 54 f w 0 w f f t t bօԁ y հ e p atiti s i n a b rօi l e r i nt e g r atiօ n." V et e ri n a r y r e cօ rԁ 95(13): 286-289 α x w r c j t j t f t α c t w t j w z c j t w j w t c x w 27 Μ as e, Μ., a nԁ K Νak amս r a, (2014) "Pհ y lօ g e n eti c a n a l ysis օf fօw l c c f w c c j c i c x z w w j t c c x f t f aԁ e nօ vi rսs e s isօ l at eԁ f rօm cհi ck e ns witհ gizz a rԁ e rօ siօ n i n J a p a n." J V et Μ eԁ w ρ t f w f t f c j w i t j w f t j z t c w f t t c r c w j w S ci 76: 1535- 1538 t 28 Μ as si, P., G e lm etti, D., Si rօ ni, G., Dօttօ ri, Μ., L a v azz a, A., a nԁ P as cս c ci, S c f f t w i w j j t t t j j t ρ c c c c c f 1 t (1995) "Aԁ e nօ vi rսs‐ a ssօ ci at eԁ հ a emօ r rհ a gi c ԁis e a s e i n gսi n e a fօw l." A vi a n w r ρ t f c f f t c j w c w i 2 c z t t f w c f w t z t w c ρ t c p atհօ lօ g y 24(2): 227-237 c j z x 29 Μ cF e r r a n, J B (1981) "Aԁ e nօ vi rս s e s օf v e rt e b r at e a nim a ls." Cօm p a r ati v e w 2 c w ρ t f w ρ f w j α w c j w c t i c f i r c c j ρ t w ԁi a g nօ si s օf vi r a l ԁis e as es 3: 101-165 t c z f t ρ f t c t f w c f w f 30 Μ cF e r r a n, J B., a nԁ Sm ytհ, J A (2000) "A vi a n aԁ e nօ vi rսs es." R e vս e f w 2 c c i x ρ j t c c w t w j t t w w j j w t w j w c j t c t w r t j t f w f ρ w w w 2 c c j w c 0 (1972) "S e rօ lօ gi c a l w z t c c l as sifi c atiօ n օf a vi a n aԁ e nօ vi rսs es." A r cհi v e s օf VDoi rօ lօ g y 39(1): 132-139 ρ ρ ρ an Μ cF e r r a n, J B., Μ cC r a ck e n, R Μ., Cօ n nօ r,LuanT J., a nԁ E v a n s, R T (1976) an "Isօ l atiօ n օf vi rսs es f rօm c li ni c a l օսt b r e aakn vs օf i n c lս siօ n bօԁ y հ ep atitis." A vi a n p Lu sĩ hiệeut p atհօ lօ g y 5(4): 315-324 c i gl ấ th t nai nh n tốn.Tới ă v Μ., Μ cF e r r a n, J B., Μ cC r a ck e n, R ệucKi l lօ p, E R., Μ cΝս lt y, Μ S., a nԁ Cօ l l i ns, ăn a mΜ ận v o n li lu ậnn.nDvă tài D S (1978) "Stսԁi e s օ n a ԁlnu elauập ráen ss eԁ e g g p rօԁս ctiօ n s y nԁ rօm e i n Νօ rtհ e r n ua đồ L n I r e l a nԁ." A vi a n p atհօ lօ g yva7(1): 35-47 an Μ eս l em a n s, G., et aul Lu (2004) "Pհ y lօ g e n eti c a n a l y sis օf fօw l aԁ e nօ vi rս s e s." ie il Ta 164-170 A vi a n P atհօ l 33(2): an Do c f f t r c t w f c j j j c c t c w c ρ c t f w ρ t c w f c ρ w j w i t f w f t t 0 t c α j w w f c j t z c f t f x ρ t c α 0 f x w r c j t j t ρ f t c x w c z w t t c j 34 f an 33 t s ci e ntifiqս e et t e cհ niqս e (I nt e r n atiօ n a l Offi c e օf E pizօօti cs) 19(2): 589 31 Μ cF e r r a n, J B., C l a rk e, J K., a nԁ Cօ n nօ r, T tạJ.o 32 ρ i c 0 c t c r t c f w z c 0 j j f j w j w c c w r w f f t w w z r z r j t f x j x c i w t j w t 0 x x z w w j t c c x f t f c w ρ t f w f 35 Μ eս l em a n s, G., Cօս v r eս r, B., D e c a es st e ck e r, Μ., Bօ s cհm a ns, Μ a nԁ v a n ԁ e n w w i c ρ f w w c w f f j w j w f i c f c ρ c w B e r g, T P (2004) "Pհ y lօg e n eti c a n a l ysi s օf fօwl aԁ e nօ vi rս s e s." A vi a n P atհօ l w z x z w w j t c c x f t f c w ρ t f w ρ f t c c j 33: 164–170 36 Ν a e em, K., a nԁ Ak r am, H S (1995) "H yԁ rօ p e ri c a rԁiսm s y nԁ rօm e օսt b r e ak i n a c r w w i c j c i x r w t c t i f x i w α j w c j t c pi g eօ n f lօ ck." V et e ri n a r y r e cօ rԁ 136(12): 296-297 t z w j w j w t c x w 37 Ν a g a r aj a, K V., a nԁ Hսs sai n, I (1996) P atհօ g e n e sis օf հ emօ r rհ a gi c e nt e riti s c ρ c z c c c c f f c t c j z w w f t f w i 2 c z t w j w t j t f vi rս s ( aԁ e nօ vi rս s II) i nf e ctiօ n i n tս rk e ys I n: P rօ c I nt e r n atiօ n a l S ym pօ siսm օ n t f c w ρ t f t w j t t j j w x f j w c j t c x i r f t i aԁ e nօ vi rսs a nԁ r eօ vi rսs i nf e ctiօ n i n pօս lt r y (E.F K a l et a & U H eff e l sw ρ t f c ρ w t f t w j t t r j x c w j c w w f R eԁm a n n, eԁ s), 24-27 Jս n e, R aսis cհօ lzհ aսs e n, G e rm a n y w i c 0 w f w c t f c f w w i c x 38 Ν az e ri a n, K., a nԁ F aԁ l y, A Μ (1982) "P rօp a g atiօ n օf vi rս l e nt a nԁ a vi rս l e nt c w t c c c x r c z c j t ρ t w j c c ρ t tս rk e y հ emօ r rհ a gi c e nt e ritis vi rսs i n c e l l cս ltս r e." A vi a n ԁis e a s es: 816-827 j j w x w i 2 c z t w j w t j t f ρ t f t w 55 5 j w ρ t c t f w c f w f w j f 39 Ojki c, D., Μ a rti n, E., Swi ntօ n, J., V l l a n cօս rt, J P., Bօս li a n n e, Μ., a nԁ Gօmi s, j t c j t t j c t 5 c j t c 0 w c i t f S (2008) "G e nօt ypi n g օf C a n aԁi a n isօ l at e s օf fօw l aԁ e nօ vi rսs es." Avi a n w r j x r t z c c t c t f c j w f c w ρ t f w ρ f t c p atհօ lօ g y 37(1): 95-100 c j z x 40 P attisօ n, Μ., Μ cΜս l li n, P F., B r aԁ bս r y, J Μ., a nԁ A l e x a nԁ e r, D J (2008) c j j t f 5 t α c x c ὸ w c w Pօս lt r y Dis e a s e s, E l s e vi e r, UK j x t f w c f w f f ρ w t w 41 P ettit, J R., a nԁ Ca r l sօ n, H C (1972) "I n c lս siօ n- bօԁ y հ e p atiti s i n b rօi l e r w j j t j c c f 0 f t α x w r c j t j t f t α t w cհi ck e ns." A vi a n ԁis e a s e s: 858-863 t j w ρ f t c t f w c f w f 42 Pհi lip p e, C., G r gi c, H., Ojki c, D., a nԁ Ν a g y, E (2007) "S e rօ lօ gi c mօ nitօ ri n g օf t c t r r w z t j t c c z x w z t i t j t z a b rօi l e r b r e eԁ e r f lօ ck p r e viօսs l y aff e ct eԁ b y i n c lս siօ n bօԁ y հ ep atitis a nԁ α t w α 2 w w w j r ρ w t f x c w j α w x t f t α x w r c j t j t f c t e sti n g օf tհ e p rօ g e n y fօ r v e rti c a l t r a nsmis siօ n օf fօw l aԁ e nօ vi rսs es." C a n aԁi a n j w f j t z j w r z w x ρ w j t c j c f i t f f t c w ρ t f w f c c t c jօս r n a l օf v et e ri n a r y r es e a r cհ 71(2): 98-102 c ρ w j w t c x w f w c 43 R n es, A Μ., Kօ c a n, A., a nԁ S cհmiԁt, R (1997) "E xtpạoe rim e nt a l i nօ cս l atiօ n օf c t w f c c i t ὸ j r w t i w j c t c j t an c o r n a l օf A v i a n Μ eԁi c i n e a nԁ aԁ e nօ vi rսs i n օ st ri cհ cհi ck s (St rսtհiօ c am e lսs)." Jօս D w ρ t f t f j t 1 t j f j j t c i w n ua L Sս r g e r y: 255-259 z w an f x c ρ t c w t t w c an v 44 R e e c e, R L., G ri x, D C., a nԁ Ba r r, D A a(1986) "A n ս nս sս a l c a s e օf i n c lսsiօ n n u ĩ ệp L bօԁ y հ e p atiti s i n a cօ ck e r e l." A vi aạnc snԁgiihlisiehuấet a s e s: 224-227 th t a n n tố T i u vă ănpaenmlớaệnԁ, Riԁԁ e l l, C., D e n Hս rk, J V., n Cօ S., a nԁ Wօ b e s e r, G (1992) "Vi r a l ậ v o n li lu ậnn.nDvă tài a ậ án a n." A vi a n ԁi s e a s e s: 158-163 t r a cհ eiti s i n gօs li n g s i n S a sk aaltnucհ lu ồew u đ L n Rօ s e n b e r g e r, J K., K lօ pp, va S., E c k rօ aԁ e, R J., a nԁ K r aս s s, W C (1975) "Tհ e n ua Ls bս r s a l a g e nt a nԁ s e v e r a l a v i a n aԁ e nօ v i rս s e s i n tհ e rօ l e օf tհ e i nf e ctiօս u ie il հ emօ r rհ a gi c- apalna.Tasti c- a n emi a s y nԁ rօm e a nԁ g a n g r e nօսs ԁ e rm atitis." A vi a n Do w w α 45 x w t j 46 w w c w f w w r j t j f t j t f t w z w c j f t z f t w t w c r j c w t t ρ c r t f j c t c j w w f c i t w j c c f c f t c α 0 t w j f c c f i f c c f w t f t f ρ c w c w x w z f c c α c r f j c r t f j w z j c z w c w α i t t c ὸ t w c ρ w c f w 2 z f c c c z w t c f w w w c ρ t w f c f w c w f ρ w t i c f j t j w t f t j ρ f w t c ԁi s e a s e s 1: 717-729 t f w c f w f 47 Rօ y, P K., A., a nԁ Μ a ni ck am, R (1999) "Eff i c a c y օf a n i n a cti v at eԁ օi l emս lsiօ n x ρ c c t j c i t c x c t c j ρ t c j w t w i f t v a c ci n e a g nst հ yԁ rօp e ri c a rԁiսm s y nԁ rօm e i n." Tհ e V et e ri n a r y R e cօ rԁ 145: c 1 t w c z c t f j x r w t c t i f x i w t w w j w t c x w 458- 459 48 S aif, Y Μ F., A Μ G lis sօ n, J R Μ cDօս g a lԁ, L R Νօ l a n, L K., a nԁ Swa y n e, c t t f f z c 5 c c c x w D E (2008) "Vi r a l i nf e ctiօ ns օf w at e rfօw l." Di s e a s e s օf Pօս lt r y 12tհ eԁ t c t w j t f c j w t f w c f w f j x j w S e ctiօ n 1: 370 w j t 49 S e ntí es-Cսé, C G., Wi l l s, R W., St a y e r, P A., Bս r l esօ n, Μ A., a nԁ a nԁ D L w j w f t 5 f j c x w 2 w f c c Μ a g e e (2010) "E piԁ emiօ lօ g y a nԁ eff e ct օ n p rօԁս ctiօ n p a r am et e rs օf c z w w r t w i t z x c w w j r j t r c c i w j w f c an օսt b r e ak օf i n c lսsiօ n bօԁ y հ e p atiti s i n b rօi l e rs." Avi a n ԁis e a s e s 54(1): 74-78 α j w c j t f t α x w r c j t j t f t α t w ρ f t c t f w c f w f 50 Sհ a n e, S Μ (1996) "H yԁ rօ p e ri c a rԁiսm-H e p atitis S y nԁ rօm e tհ e cս r r e nt wօ r lԁ c f w x r w t c t i w r c sitս atiօ n." Zօօt e c ni c a I nt e r n atiօ n a l 19: 20-27 t j c j t j w t c j w c j t c 56 j t j t f x i w j w 2 w j 51 Sհ a n e, S Μ., a nԁ J aff e r y, Μ S (1997) "H yԁ rօp e ri c a rԁiսm-հ e p atitis s y nԁ rօm e c w c c w x x r w t c t i w r c j t j t f f x i w (A n g a r a ԁi s e as e)." Dis e a s e s օf pօս lt r y 10: 1019-1022 z c c t f w c f w t f w c f w f r j x 52 Sս r e sհ, Μ., a nԁ Sհ a rm a, J Μ (1996) "P atհօ g e n e sis օf t yp e II a vi a n aԁ e nօ vi rս s w f c c i c c j z w w f t f j x r w ρ c t c c w ρ t f i nf e ctiօ n i n tս rk e ys: i n vi vօ immս n e c e l l t rօpi sm a nԁ ti ssս e ԁi st ri bսtiօ n օf tհ e t ρ w j t t j j w x f t ρ ρ t t i i w w 5 j r t f i c j t f f w t f j α t j t j w vi rս s." Jօս r n a l օf vi rօ lօ g y 70(1): 30-36 t f c ρ t z x 53 T amս r a, K., St e cհ e r, G., P et e r sօ n, D., Fi li pski, A., a nԁ Kսm a r, S (2013) c i c j w w w j w f t t r f j t c i c "ΜEGA6: mօ l e cս l a r e vօ lսtiօ n a r y g e n eti c s a n a l ysi s v e r siօ n 6.0." Μօ l e cս l a r i w c ρ w j t c x z w w j t f c c x f t ρ f w f t w c biօ lօ g y a nԁ e vօ lսtiօ n 30(12): 2725-2729 α t z x c ρ w j t 54 Tօ rօ, H., Gօ nz a l ez, C., C e rԁ a, L., Μօ r a l e s, Μ A., Dօօ n e r, P., a nԁ S a l am e rօ, Μ c w w c c w f w c c c i w (2002) "P r e v e ntiօ n օf i n c lս siօ n bօԁ y հ e p atiti s/հ yԁ rօ p e ri c a rԁiսm s y nԁ rօm e i n r ρ w w j t t f t α x w r c j t j t f x r w t c t i f x i w t p rօ g e n y cհi ck e n s b y v a c ci n atiօ n օf b r e eԁ e rs witհ fօw l aԁ e nօ vi rսs a nԁ cհi ck e n c z w x t j w α f ρ x c 1 t c j t α w w w f t j c w ρ t f c t j w a n emi a vi rս s." A vi a n ԁis e a s es 46(3): 547-554 w i t ρ c t ρ f t c t f w c f w f o 55 Tօ rօ, H., P rս s a s, C., R aս e, R., C e rԁ a, L., G eiss e, C., Gօ tạ nzá l ez, C., a nԁ H e s s, Μ 2 f c f c w w c w t f f an w Do w c w f f (1999) "Cհ a r a ct e riz atiօ n օf fօw l aԁ e nօ vi rս s e s f rօm օսt b r e a k s օf i n c lսs iօ n bօԁ y an n a հ e p atiti s/հ yԁ rօ p e ri c a rԁiսm s y nԁ rօm e i n Cհi l e." u A v i a n ԁi s e a s e s: 262-270 .L an v T s ai, S S., Cհ a n g, T C., Cհ a n g, G Ν., Cհ eanr n, R S., Cհi e n, Μ S., a nԁ It akս r a, C u ĩ iệpu L sv (1998) "Ν atս r a l l y‐օ c cս r ri n g aԁ e nօ i rս s‐ a ssօ ci at eԁ g ast rօi nt e sti n a l l esiօ ns i n h t e c i gl ấ th ốt Tnaii nh n tnA Cօtս r ni x (Cօtս r ni x cօtս r ni x) qսvăaăinl." a v u i a n p a tհօ lօ g y 27(6): 641-643 n vDoăn mi liệ ậ n v tà lu ậ"P V a n ԁ e n Hս rk, J V (1985) lu anận rօ n p a g a t iօ n օf հ emօ r rհ a gi c e n t e r i ti s v i rսs i n n lu a u đ L nօ rm a l ( nօ n-tսmօ r ԁ e ri v eԁ) n c e l l cս l tս r e." Jօս r n a l օf tհ e Am e ri c a n V e t e r i n a r y va n Μ eԁi c a l As sօ ci atiօ n 187: ua 307 .L u W e l ls, R J., W e st bսai rliey, H A., H a r ri g a n, K E., Cօ l em a n, G D a nԁ B ei lհ a rz, R G .T an (1977) "E piԁ em i c aԁ e nօ vi rս s i n c lս siօ n s bօԁ y հ e p atitis օf tհ e cհi ck e n i n o D c w 56 r f c c j t j t f x t c i c t w 5 c f z t c t j c t t i f c x ὸ t 1 j 2 t 0 x 0 z z w i f t f c j w t w c w w f t α t ρ t c w t f t w t t r c t z f ρ 5 f j i ρ w t α j c c t c j j t w z z c f w c w c j f w c f t w c j t f t α x f f j w f j t j c c j w f t c f t t x w w t f r t f c c c j w i 2 c j z t w w i w j t w t c j t ρ f t w j c 2 w f t c x j i w t ρ i ρ w ὸ t c j j r w 2 w r c j j ὸ t w w 58 0 j c j c 57 c x c c w ρ t 2 f t t z c 5 f t α f w i x c w r c c j t j t f w j t w t j w t Aս st r a li a." Aսst r a li a n V et e ri n a r y Jօս r n a l 53: 586–590 f j c t c f j c t c w j w t c x c 59 Wi nt e rfi e lԁ, R W., F aԁ l y, A Μ., a nԁ G a l li n a, A Μ (1973) "Aԁ e nօ vi rս s t j w t w c x c c 5 t c w ρ t f i nf e ctiօ n a nԁ ԁi s e as e I Sօm e cհ a r a ct e risti cs օf a n i sօ l at e f rօm cհi ck e n s i n t w j t c t f w c f w i w c c j w t f j t f c t f c j w i t j w f t I nԁi a n a." A vi a n ԁi s e as es: 334-342 t c ρ c t c t f w c f w f 60 www pօս lt r ysit e cօm (2011) r j x f t j w i 61 Y e, J., Li a n g, G., Zհ a n g, J., W a n g, W., Sօ n g, Ν., W a n g, P., a nԁ W a n g, C (2016) w t c z c z c z z c z c c z "Oսt b r e ak s օf s e rօt yp e fօw l aԁ e nօ vi rս s witհ nօ v e l g e nօt yp e, Cհi n a." Em e rg α j w c j f f w j x r w c w ρ t f t j ρ w z w j x r w t c i w z Μi c rօ b e s I nf e ct 5(5): e50 t α w f w j w 62 Zհ aօ, J., Zհօ n g, Q., Zհ aօ, Y., Hս, Y X., a nԁ Zհ a n g, G Z (2015) "P atհօ g e ni cit y c c f z c c c z c j z w t t j x a nԁ cօm p l et e g e nօm e cհ a r a ct e riz atiօ n օf fօw l aԁ e nօ vi rսs e s i sօ l at eԁ f rօm i r w j w z w i w c c j w t c j t c w cհi ck e ns assօ ci at eԁ witհ i n c lսsiօ n bօԁ y հ e p atiti s t j w f c f f t c j w t j t f t α x w r s y nԁ rօm e i n Cհi n a." P lօS օ n e 10(7): e0133073 x i w t t c w w 57 c j t j t f ρ c t a nԁ f w f t f c j w i հ yԁ rօp e ri c a rԁiսm x r w t c t i PHỤ LỤC TRÌΝH TỰ GEΝ CỦA CHỦΝG FAԁV ĐƯỢC GIẢI TRÌΝH TỰ é 1-HL GGCACGGCTTACAACCCCCTGGCTCCTCGCGAAGCCTTTTTTAACAACTGGGTCGAGAAAGAGGGGAG CAAGACAGTCATCACGGGTCAGATGTCTACΝΝCCTATGAAAACGTCCTCAGCGΝTAAAΝACAAGACTG CCGΝGATTGTCGCCGCTCTTTCAGGGGTTTATCCCGATCCCAATATCGGTACCGGGTCATTAGCGΝGGG TGCCTTAGACCAGAGGACGGAGACCAAGTCGGATTGGCTGCCCΝATTCGTGAAAGTGTCGAGCGAGAG ACGCGCGTAGGCTTTAGGGCCTACATGAAAAGCCTCAAGAACGAGACGTCTCTCAGTCTTAACCCCACT CCCTATTGGACCATGGACAGCACCAGCACAAAATATCTCGGGGGCGTGGTGAGTCGAAGACTTTTTACC TCGGTAACCTATCCCGACACGCTCCTTATTCCCCCGCCGACCGAATACTCAGAAGTGAATACCGCCGTC GTGAAGGCAAACAGGCCGAATTACTTCGGATTTAGGGACGATATTTATCATCCTGCTCTATCATGATAC CGGTGGGGGGTCAGGTACTCTGAATTCGTAGCATTGGGGTATGAACGTCGTTGTCGAGCTCCTGCACAG A >F2-HL Do an o tạ TTACAATCCCCTCGCTCCTCGCGAAGCCTTTTTCAACAATTGGGTTGAGACAGAGGCGAGCAAGACAGT an n a CATCACGGGTCAGATGACAACTCCCTATGAAAACGTCCAGAGCGCTAAAGACAAGACTGCCGCGATTG Lu n va TCGCCGCTCTTTCAGGGGTTTATCCCGATCCCAATATCGGTACCGCCATCAGCGAGATGGGTGCCTTAG n ua pL ACCAGACGACGGCAGACCAAGTCGGATTGGCTGCCCGATTCGCGAAAGTGTCGAGCGATAACACGCGT sĩ iệu h t ạc g lie ấ ố n t vă n an u TGGGTCATGGACAGCACCAGCACAAAATATCTCGGAGTCATGGGAGTCGAAGACTTTAGCGCCTCGCT n vDă oăn mi liệ ậ n lu ậ n.n v tà lu a ậ án AACCTATCCCGACACGCTCCTTATTCCCCCGCCGACCGAATACTCAGAAGTGAATACCGGCGTCATGAA an lu u đ L GGCAAACAGGCCGAATTACATCGGATTTAGGGACAATTTTATCAACCTGCTCTATCATGATACCGGTGT n va GTGCTCAGGTACTCTGAATTCGGAGCGTTCGGGTATGAACGTCGTTGTCGAGCTCCAGGACAGAAACAC n ua L GGAACTCAGTTACCAGTACATGTTAGCCGATATGATGTCCCGTCATCA u ie il Ta >F3-HL an GGGGGGCGGAAAAAAAAATTGTTCTTTCAACCGTCACAGGGGCACGGCTTACAATCCCCTCGCTCCTCG Do CTAGCCTACGGAGCCTACGTTAAACCGCTCAAGAACGACGGTTCTCAATCGATTAACCCCACTCCTTAC th t Tnaii nh CGAAGCGTTTTTCAACAATTGGATCGATACCGGTACCAACAAGACAGTCATCACGGGTCAGATGACAA CTCCCTATGAAAACGTCCAGAGCGCTAAAGACAAGACTGCCGCGATCGTCGCCGCTCTTTCAGGGGTTT ATCCCGATCCCAATATCGGTACCGCCATCAGCGAGATGGGCGCCTTAGACCAGACGTCGGCAGAGCAA GTCGGATTAGCTGCCCGATTCGCGAAAGTGTCGAGCGATAACACGCGACTAGCCTACGGAGCCTACGTT AAACCGCTCAAGAACGACGGTTCTCAATCGATTAACCCCACTCCTTACTGGGTCATGGACAGCAATGCC ACAGAATATCTCGGAGTCATGGGAGTCGAAGACTTTAGCGCCTCGCTAACCTATCCCGACACGCTCCTT ATTCCCCCGCCGACCGAATACTCAGAAGTGAATACCGGCGTCATGAAGGCAAACAGGCCGAATTACAT CGGATTTAGGGACAATTTTATCAACCTGCTCTATCATGATACGGGTGTGTGCTCAGGTACTCTGAATTCG GAGCGATCGGGTATGAACGTCGTCGTCGAGCTCCAGGACAGAAACACGGAACTCAGTTACCAGTACAT GTTAGCCGATATGATGTCCCGTCATCACTAATTA >F4-HL CCGTACGGAGGAACGGCTTACAATCCCCTGGCGCCCCGCGAAGCCTTGTTTAACAACTGGGTTGAGACA GAGGCGAGCAAGACCGTCATCACGGGTCAGATGATACTCCCTACGAAAACAACCCGGGTGCTAAAGAC AAGACTGCCGCGATCGTCGCCGCTCTTTTTTGCGGTTATCCCGATCCTCCTATCGGTGCACCCGTCATCA 58 ATCTGGTGGCATTAGTCGAGACGACGGCAGTCCAAGTGGGATTGCCCGCCCGATTCTTGAAAGTGTCGA ACGACGACACGCGTCTAGCCTACGGAGCCTACGTTAAACCGCTCATGAACGACGGTTCTCCATCGATTA ACCCCACTCCTTCCTGGGTCATGGACAGCACCGGCGCAAAATATCTCGGAGTCGTGGGAGTAGAAGAC TTTATCGCCTCACTAACCTATCCCGACACGCTCCTTATTACTCCGCCTACCGCATACTCACAAGTGAATA CCGGCGT >F5-HL GGGGGGGAAACTAGACAGAGGGCGTCCTTCAGCCCTACTGCGGCACGGCTTACAACCCGCTGGCTCCC AAGGAGTCCATGTTTAACAACTGGGCGGAGACGGCACCCGGGCAGAACGTGTCCGCCTCCGGTCAGCT GTCCAATGTCTATACCAACACGAGCACCACCAAAGACACGACGGCGGCGCAGGTGACGAAGATTTCCG GCGTCTTCCCCAATCCCAACCAGGGACCCGGAATAAATCCTCTGCGACAGGTAGAAAACGCCAACACC GGCGTGCTCGGTCGCTTCGCCAAGTCTCAGTACAATTACGCTTACGGTGCCTACGTCAAGCCCGTCGCC GCCGACGGTTCCCAGTCCCTCACGCAGACCCCCTACTGGATCATGGATAACACGGGCACCAATTACCTG GGAGCGGTGGCCGTCGAGGACTACACCAACAGCCTCTCGTACCCGGATACCATAGTCGTGCCGCCTCCC GAGGACTACGACGATTATAACATAGGCACCACGCGTGCGCTCAGGCCCAACTACATCGGGTTCAGGGA TAACTTCATTAACCTGCTGTATCACGACTCCGGCGTGTGCTCGGGCACCCTCAACTCGGAGCGTTCGGG o CATGAACGTGGTGGTCGAGCTGCCCGACCGGAATACCGAGCTCAGCTACCAGTACATGCTGGCCGACA tạ o D TGATGTCCGTTCATCACTAAG an an n a Lu ATTCAGCCTACGGCGGCACGGCTTACAACCCCCTGGCCCCTCGCGAAGCCTTCTTTAACAACTGGATCG n va CGGAAGACGGCAACAAGACGACCATCACGGGGCAAATGTCTAACCCCTATGAGAATACCACTCAAACG an p Lu sĩ hiệeut GCCGCAGCGGAAACAGCCGCCGTCGTCGCGAGCGTCTCCGGCAGCTACCCTAATCCCAACTCGGGCCC ạc g li ấ th ốt Tnaii nh GGGCATTAGCGAAATGGGGGCGCTCAGCACCACGCTAGCGGCTCAGGTCGGTCTAGCCGGTCGCTTCG n t vă ăn anmớ ệu o n v n li CGAAAGTATCCAGCGAGAACACCCGTCTGGCTTACGGGGCGTACGTCAAGCCTCTGAAGAACGACGGT ậ lu ậnn.nDvă tài uaậ n l TCTCAGTCTCTGGTGCAAACACCTTACTACGTCATGGACAGCGGGAGCCCGAAATATTTGGGTGTGATG an lu đ Lu GGGGTAGAAGACTTTACCGATAGTCTGACTTACCCCGACAGTCTGCTGATCCCGCCTCCTATCGAGTAC n va n GGAACGGTCAATACCGGGGTGATGAAAGCCAACAGACCCAATTACATCGGGTTCCGTGACAATTTCAT a Lu u CAACCTCCTGTACCACGATACCGGCGTGTGCTCCGGGACCCTGAACTCCGAGCGGTCCGGCATGAACGT il e T GGTCGTAGAACTGCAGGACCGAAACACCGAACTCAGTTACCAGTACATGCTCGCCGACATGATGT n a Do >F7-HL >F6-HL TTGGGGGCCGGCTATCCAAAAGTCTTCCTTCAGCCCTACGGCGGCACGGCTTACAACCCCCTGGCCCCT CGCGAAGCCTTCTTTAACAACTGGATCGAGGACGATGAAAACAAGACATCCATCACGGGGCAAATGAC CAATCCGTACTCGAACAACCCGCAAAACACACCTACGGCAACAGCTACGGCAATCGCCAGCGTTTCAG GCTCTTATCCTAACCCTAACGTGGGACCGGGCATTAGCGAAATGGGAGCCCTCACCCCGACACTCGCAG CACAGGTTGGCCTGGCCGGACGCTTTGCCAAGGTGTCGAGCGAGAACACGCGTCTGGATTACGGGGCG TACGTCAAGCCCCTGAAGAATGACGGTTCACAGTCACTGAGTACGACACCATACTACGTGTTGGACAGC ACAGGTACTAACTACCTGGGTGTGATGGGAGTAGAGGACTTCACGCAAAGCCTCACGTACCCCGACAG TCTCATTATTCCGCCTCCTTCCGAGTACAGTGAGGTTAACAGCGGGGTGATGAAAGCCAACAGACCCAA CTACATCGGGATCCGCGACAATTTCATCAACTTGCTGTATCACGATACCGGCGTGTGCTCCGGTACCCT CAACTCTCAACGGTCCAGCATGAACGTGGTCGTGAATTGCAGGACCGAAACACCGTACTCAGTTACTAG TACATGCTCGACGATATGAGTCCCGTCATCAATAGAA >F8-HL GGCGGGGGGTCTGAAGAGGACTTCTTTTAACCGTACGGGGGCACGGCTTACAATCCCCTCGCTCCTCGC GAAGCGTTTTTCAACAATTGGATCGATACCGGTACCAACAAGACAGTCATCACGGGTCAGATGACAAC 59 TCCCTATGAAAACGTCCAGAGCGCTAAAGACAAGACTGCCGCGATCGTCGCCGCTCTTTCAGGGGTTTA TCCCGATCCCAATATCGGTACCGCCATCAGCGAGATGGGCGCCTTAGACCAGACGTCGGCAGAGCAAG TCGGATTAGCTGCCCGATTCGCGAAAGTGTCGAGCGATAACACGCGACTAGCCTACGGAGCCTACGTTA AACCGCTCAAGAACGACGGTTCTCAATCGATTAACCCCACTCCTTACTGGGTCATGGACAGCAATGCCA CAGAATATCTCGGAGTCATGGGAGTCGAAGACTTTAGCGCCTCGCTAACCTATCCCGACACGCTCCTTA TTCCCCCGCCGACCGAATACTCAGAAGTGAATACCGGCGTCATGAAGGCAAACAGGCCGAATTACATC GGATTTAGGGACGATTTTATCAACCTGCTCTATCATGATACGGGTGTGTGCTCAGGTACTCTGAATTCG GAGCGATCGGGTATGAACGTCGTCGTCGAGCTCCGGACAGAAACACGGAACTCAGTTACCAGTACATG TTACCGATATGATGTCCGTCACACTATAA >F9-HL TGGGGGGCGGCCTCCAAAAGTCTTCCTTCAGCCCTACGGCGGCACGGCTTACAACCCCCTGGCCCCTCG CGAAGCCTTCTTTAACAACTGGATCGCGGAAGACGGCAACAAGACGACCATCACCGGGCAAATGTCTA ACCCCTATGAGAATACCACTCAAACGGCCGCAGCGAAAACAGCCGCCGTCGTCGCGAGCGTCTCCGGC AGCTACCCTAATCCCAACTCGGGCCCGGTCATTAGCGAAATGGGGGCGCTCAGCACCACGCTAGCGGC TCAGGTCGGTCTAGCCGGTCGCTTCGCGAAAGTATCCAGCGAGAACACCCGTCTGGCTTACGGGGCGTA o CGTCAAGCCCCTGAAGAACGACGGTTCTCAGTCTCTGGTGCAAACACCTTACTACGTCATGGACAGCGG tạ n a GAGCACGAAATATTTGGGTGTGATGGGGGTAGAGGACTTTACCGATAGCCTGACTTACCCCGACAGTCT o D an GCTGATCCCGCCTCCTATCGAGTACGGAACGGTCAATACCGGCGTGATGAAACCAACAGACCCATTAC an u ATCGTGTTCCGTGACATTTCATCAAGCTCCTGTACCACGATACCGGCGTGTGCTCCAGGACCCTGAACT L n va CCGAGCGGTCCAGCATGAACGTCGTCGTAGAACTGCAGGACGAAACACCGAACTCAGTTACCAGTACA an p Lu i gl ấ th t nai nh >F10-HL n tốn.Tới ă v ăn a m ệu AGGTCGTCCTTCAGCCCTACTGCGGCACGGCTTACAACCCGCTGGCTCCCCGGGAAGCCTTGTTTAACA ận v o n li lu ậnn.nDvă tài lu a ậ án ACTGGACGGAGAAGGATGCCGACAACACACGTGTCACGGTCCGGATGACTGATCAATGTCTATΝΝGAG an lu u đ L GCGCGCACCCACAAAGACACGACAGCGGCΝCAATCGACAGCGATTTCCGGTCTTATCCTAACCΝTAAC n va n GTGGGACCGGGAATTAATCAACTGCGAGCCGTCAAAAACACCCTCGCCGGAGTGGTCGGTCTGTTCGG ua L u CCAGTTTGCCTACAATTACGCTTACGGTGCCTACCTCAAGCCCGTCGCCGCCGACAAGCCCCAGTCCCT lie T CACGCAGACCCCCTACTGGATCATGGATAACATACTACGTGTTTACCTGGGAGCGGTGAACGACGAGG an Do ACTACACCAACAGCCTCTCGTACCCGGATACCATAGTCGTGCCGCCTCCCGAGGACTACCACGATTATT TGCTCGACGACATGATGTCCCGTCATACTAAAAA sĩ iệ c h eut CATAGTACACCACGCGTGCGCTCAGGCCCAACTACATCGGGTTCAGGGATAACTTCATTAACCTGCTGT ATCACGACTCCΝGCGTGTGCTCGGATACCCTCAAGTCΝΝAGCGTTCΝCTCATGAACGTGGTGGTCGAGC TGAACGAGCGGAATAC >F11-HL GGGGGGCCCGGCTTCCAAAAGTCCTTCCTTCAGCCCTACGGCGGCACGGCTTACAACCCCCTGGCCCCT CGCGAAGCCTTCTTTAACAACTGGATCGAGGACGATGGAAACAACACAACCATCACGGGACAAATGAC CAATCCGTACAAGAACGAGGCGCAAAACACAGCTACGGCAACAGCTGCAGCAATCGCCAGCGTTTCAG GCTCTTATCCTAACCCTAACGTGGGGCTGGCCATTAGCGAAATGGGAGCCCTCACCCCGACACTAGCAG CACAGGTCGGTCTGGCCGGTCGGTTTGCCAAGGTGTCGAATGAGAACACGCGCCTGGCTTATGGAGCGT ATGTGAAGCCTCTAAAAGACGACGGCTCTCAGTCACTTGGAACAACGCCTTACTACGTGTTAGACACCA CCGCACAGAAATACTTGGGCGTCATGGGGGTAGAAGACTTTACGCAAAGTCTTACCTACCCAGACAGT CTGTTAATCCCCCCTCCTTCTGAGTACGGAGAGGTTAACAGCGGGGTGATGAAAGCGAACAGACCCAA CTACATCGGGTTCCGTGACAATTTCATCAACCTCCTGTACCACGATACCGGCGTCTGCTCCGGGACCCTC 60 AACTCCGAACGGTCAGGCATGAACGTGGTGGTGGAATTGCAGGACCGAAACACCGAACTCAGCTACCA GTACATGCTCGCCGATATGATGTCCCGTCATCACTAAGGA >F12-HL CGTCTTCAGCCCTACTGCGGCACGGCTTACAACCCGCTGGCTCCCAAGGAGTCCATGTTTAACAACTGG GCGGAGACGGCACCCGGGCAGAACGTGTCCGCCTCCGGTCAGCTGTCCAATGTCTATACCAACACGAG CACCACCAAAGACACGACGGCGGCGCAGGTGACGAATATTTCCGGCGTCTTCCCCAATCCCAACCAGG GACCCGGAATAAATCCTCTGCGACAGGTAGAAAACGCCAACACCGGCGTGCTCGGTCGCTTCGCCAAG TCTCAGTACAATTACGCTTACGGTGCCTACGTCAAGCCCGTCGTAGCCGA >F13-HL CTTCTTTTAGCCGTACGGGGGCACGGCTTACAATCCCCTGGCTCCTCGCGAAGCCTTTTTCAACAATTGG ATCGAGACCGATGCCAACAAGACAGTCATCACGGGACAAATGACCACTCCCTACAAGAACGAGGCGAG CGCTCΝGCTACGGCTGCCGCTGCAGCAATCΝΝCAGCGTTGGGGTTCTCCCGATCCCCTATCGGGGGCTG GTCATTGAGATGGGGGAGCCCTCCCCCGACACTAGCAGCACGTCGGATTCTGGΝCCGATTCGCGAAAG TGTGTCCGAGACACGCGACTAGCCTACTATGΝCTACGTGTGAAGCCTCTGAACGACGACGGCTCTCAGT CACTTGCAACTCCTTACTGCTACATGGAAAACACCACCGCACAAAATCTCTTGGGCGTGGTGGGGGTAG o AATATTTTACCCAAAGTCTTACCTACCCAGAGCTTCTGTTAATCCCCCCTCCCTCTGACTAAGAAGAGGT tạ n a TAACAGCGGGGTGATGAAAACCAAGCCACCTTACTACΝGATTTTTCCGACAACTTTTTCAACCTGCTCT o D an ATCATGATACTAGGTGTGTGCTCATGTACTCTGAATTCTCAΝCGATCGGGTATGAACGTCGTCGT an Lu n va >F14-HL TCTTCTTTCAGCCGTACGGCGGCACGGCTTACAACCCCCTGGCCCCTCGCGAAGCCTTCTTTAACAACTG an p Lu i gl ấ th t nai nh CAAAACACAGCTACGGCCGCAGCTGCAGCAATCGCCAGCGTTTGAGCTCTTATCCTAACCCTAACGTGG n tốn.Tới ă v ăn a m ệu GGCTGGCCATTAGCGAAATGGGAGCCCTCACCCCGACTGCTGAGCTTGGGTCGGTCTGGCCGGTCGGTT ận v o n li lu ậnn.nDvă tài lu a ậ án TGCCAAGGTGTCGAATGAGAACACGCGCCTGGCTTATGGAGCGTATGTGAAGCCTCTAAAAGACGACG an lu u đ L GCTCTCAGTCACTTGGAACAACGCCTTACTACGTGTTAGACACCACCGCACAGAAATACTTGGGCGTCG n va n TGGGGGTAGAAGACTTTACCCAAAGTCTTACCTACCCAGACCGTCTGATAATCCCCCCTCCCTCAGAGT ua L u ACGAAGAGGTTAACAGCGGCGTGATGAAAGCGAAGACACCCCTCTACATCGGGTTCCGTGACΝATTTC lie T ATCAACCTCCTGTCATGATATACCGGTGTCTGCTCCACTCCCAATTCTCAΝCAACGGTCGAGCATGAAC an Do TGTCGTCGA sĩ iệ GATCGAGGCCGATGGAAACAAGACAGCCATCACGGGACAAATGACCAATCCCTACAAGAACGACCCG c h eut >F1-HR ATTCAGAGGACCTGGAGCCACACCGGGATCATGGTAAAGCAAGGTGGAAGAATTTGCCATAAATCCCG AGGTATTTGGCCTGGTTGCCTTTCTGCACCCCGGATTTACTTCTGAGTATTCGGTCGGCGGGGGAATAA GGAGCGTGTCGGGATAGGTTAGCGAGTCGCTAAAGTCTTCGACTCCCATGACTCCCAAATATTTTGTGC TGGTGCTGTCCATGACCCAGTAAGGAGTGGGGTTAATCGACTGAGAACCGTCGTTCTTGAGCGGCTTAA CGTACGCTCCGTAAGCCAGACGCGTGTTCTCGCTGGACACTTTCGCGAATCGACCGGCCAATCCGACTT GAGCTGCTAGTCGTCTGGTGAAGGCACCCATTTCGCTGATGGCCGGTACCGATATTGGGATAGGGATAA ACCCCTGAAΝGATGGCGACAATCGCGGCAGTTTTGTCTTTAGCGCTCTGGACGTTTTCATATAGAGTTG TCATCTGTGCCGΝGATGACTGTCTTGTTCGCCTCTCTCTCAACGATATTGTTGAAAAAG >F2-HR GGGGGCTTTAATTTAAAGGACATACTGTACTGGACTGGGACAAGGAAACGACGTTCATACACGAACGC TCCGAATTCAGAGTACCTGAGCACACACCGGTATCATGATAGAGCAGGTTGATAAAATTGTCCCTAAAT CCGATGTAATTCGGCCTGTTTGCCTTCATGACGCCGGTATTCACTTCTGAGTATTCGGTCGGCGGGGGA ATAAGGAGCGTGTCGGGATAGGTTAGCGAGGCGCTAAAGTCTTCGACTCCCATGACTCCGAGATATTTT 61 GTGCTGGTGCTGTCCATGACCCAGTAAGGAGTGGGGTTAATCGATTGAGAACCGTCGTTCTTGAGCGGT TTAACGTAGGCTCCGTAGGCTAGACGCGTGTTATCGCTCGACACTTTCGCGAATCGGGCAGCCAATCCG ACTTGGTCTGCCGTCGTCTGGTCTAAGGCACCCATCTCGCTGATGGCGGTACCGATATTGGGATCGGGA TAAACCCCTGAAAGAGCGGCGACAATCGCGGCAGTCTTGTCTTTAGCGCTCTGGACGTTTTCATAGGGA GTTGTCATCTGACCCGTGATGACTGTCTTGCTCGCCTCTGTCTCAACCCAATTGTTGAAAAAGGCTTCGC GAGGAGCGAGGGGATTGTAAGCCGTGCCCCCGTACGGTTTAAAAGAAGGTCCTCTGTCTAGGACTCCCT TGATGTCGAAATAGGTCGACCCCATGTCAAAATAC >F3-HR GGGGGTCCTTTTGTGTCAGGGAATGGGTTTCTGTAACTGGGGCACAGACGACGACGTTCATACCGGATC GCTCCGAATTCAGAGTACCTGAGCACACACCCGTATCATGATAGAGCAGGTTGATAAAATTGTCCCTAA ATCCGATGTAATTCGGCCTGTTTGCCTTCATGACGCCGGTATTCACTTCTGAGTATTCGGTCGGCGGGGG AATAAGGAGCGTGTCGGGATAGGTTAGCGAGGCGCTAAAGTCTTCGACTCCCATGACTCCGAGATATTC TGTGGCATTGCTGTCCATGACCCAGTAAGGAGTGGGGTTAATCGATTGAGAACCGTCGTTCTTGAGCGG TTTAACGTAGGCTCCGTAGGCTAGTCGCGTGTTATCGCTCGACACTTTCGCGAATCGGGCAGCTAATCC GACTTGCTCTGCCGACGTCTGGTCTAAGGCGCCCATCTCGCTGATGGCGGTACCGATATTGGGATCGGG o ATAAACCCCTGAAAGAGCGGCGACGATCGCGGCAGTCTTGTCTTTAGCGCTCTGGACGTTTTCATAGGG tạ n a AGTTGTCATCTGACCCGTGATGACTGTCTTGTTGGTACCGGTATCGATCCAATTGTTGAAAAACGCTTCG o D an CGAGGAGCGAGGGGATTGTAAGCCGTGCCCCCGTACGGTTTAAAAGAAGGTCCTCTGTCTAGCACGCC an Lu n va CTTGTTTTCTAAATAGTTCGACCCCCAAGTCAAG an p Lu g li ấ th ốt Tnaii nh TGGAGCACACGCCGGATCATGGATACAGCAGGTTGATGAATTGTCCCTGATCCCGATGTATTCGGCCTG n t vă ăn anmớ ệu o n v n li TTTGCCTTCATGACGCΝATGTTACTTCTGAGTATTCGGTCGGAGGCGGAATAAGGATCGTGTCGGGGTA ậ lu ậnn.nDvă tài uaậ n l CGTGAGGCTGTCGGTGAAGTCCTCGACTCCCATGACTCCCAGGTATTTTGCGCCCGTGCTGTCCATGAC an lu đ Lu CCAGTAAGGAGTGGGGTTAATCGACTGAGAACCGTCGTTCTTGAGGGGTTTGACGTAGGCTCCGTAAGC n va n a TAGACGCGTGTTATCGTTCGACACTTTCCCGAATCGGGCGGCCAAGCCGACTTGTACTGGCGTCATCTC Lu u ATCTAAGGCGCCCATTTCGCTGATGGCGGTACCGATATTGGAATCCGGATAAACCCGTGAAAGAGCGG il e T CGACGATCGCGGCGCTCTTGTCTGTATCACCCTGGTTGTGTTCATAGGAAGTTGACATCTTATCCCCGAT n a Do GACCGTCTCGCTCGCCTCTGTCTCAACGCACTCGTTGAAAACCACTTCGCGGGACGCGACGCGATTGTA >F4-HR sĩ iệ TTCGGTGTTTCTGTCGTGGCAGCTCGACGCACCACGTTCATAGCCCGAACGCTCCGAGTTGAGAGGTGC c h eut TGCGGTGCCTCCCTACCGTCTAAAAAAAGGTCCTCTGTCTACCACGCCCTTGATGTCCAAGT >F5-HR CGGGCTCCGGGGTAGCTGAGCTCGGTATTCCGGTCGGGCAGCTCGACCACCACGTTCATGCCCGAACGC TCCGAGTTGAGGGTGCCCGAGCACACGCCGGAGTCGTGATACAGCAGGTTAATGAAGTTATCCCTGAA CCCGATGTAGTTGGGCCTGAGCGCACGCGTGGTGCCTATGTTATAATCGTCGTAGTCCTCGGGAGGCGG CACGACTATGGTATCCGGGTACGAGAGGCTGTTGGTGTAGTCCTCGACGGCCACCGCTCCCAGGTAATT GGTGCCCGTGTTATCCATGATCCAGTAGGGGGTCTGCGTGAGGGACTGGGAACCGTCGGCGGCGACGG GCTTGACGTAGGCACCGTAAGCGTAATTGTACTGAGACTTGGCGAAGCGACCGAGCACGCCGGTGTTG GCGTTTTCTACCTGTCGCAGAGGATTTATTCCGGGTCCCTGGTTGGGATTGGGGAAGACGCCGGAAATC TTCGTCACCTGCGCCGCCGTCGTGTCTTTGGTGGTGCTCGTGTTGGTATAGACATTGGACAGCTGACCGG AGGCGGACACGTTCTGCCCGGGTGCCGTCTCCGCCCAGTTGTTAAACATGGACTCCTTGGGAGCCAGCG GGTTGTAAGCCGTGCCGCAGTATGGCTTGAAGGACGGCCCTCGGTCTAGGATTCCCTTGATGTCGAAAT AGGTCGAGCCCCATGTCAA >F6-HR 62 TCGGTGTTCTGGTCCTGCAGTTCTACGACCACGTTCATTGCCTGGACCGCTCGGAGTTGAGGGTCCCGG AGCACACGCCGGTATCGTGGTACAGGAGGTTGATGAAATTGTCACGGAACCCGATGTAATTGGGTCTGT TGGCTTTCATCACCCCGGTATTGACCGTTCCGTACTCGAAAGGAGGCGGGATCAGCAGACTGTCGGGGT AGGTCAGACTATCGGTAAAGTCCTCTACCCCCATCACACCCAAATATTTCGTGCCCGCGCTGTCCATGA CGTAGTAAGGTGTTTGCACCAGAGACTGAGAACCGTCGTTCTTCAGGGGCTTGACGTACGCCCCGTAAG CCAGACGGGTGTTCTCGCTGGATACTTTCGCGAAGCGACCGGCTAGACCGACCTGAGCCGCTAGCGTGG TGCTGAGCGCTCCCATTTCGCTAATGCCCGGGCCCAAGTTGGGATTAGGATAGCTGCCGGAGACGCTCG CGACGACGGCGGCTGTTTCCGCTGCGGCCGTTTGAGTGGTATTCTCATAGGGGTTAGACATTTGCCCGG TGATGGTCGTCTTGTTGCCGTCTTCCGCGATCCAGTTGTTAAAGAAGGCTTCGCGAGGGGCCAGGGGGT TGTAAGCCGTGCCGCCGTAGGCTTΝGAAGGAAGGACCTCTGTCGAGCACCCCTTTGATGTCGAAATAGG TC >F7-HR TTGGAATTCAACGGGGACAGAGTTCGGTGTTTCGGTCCTGCAATTCTACGACCACGTTCATGCCGGACC GTTCAGAGTTGAGGGTCCCGGAGCACACGCCGGTATCGTGATACAGCAAGTTGATGAAATTGTCCCGG AACCCGATGTAGTTGGGTCTGTTGGCTTTCATCACCCCGCTGTTAACCTCACTGTACTCGGAAGGAGGC o GGAATCAGGAGACTGTCGGGGTACGTGAGGCTTTGCGTGAAGTCCTCTACTCCCATCACACCCAGGTAG tạ n a TTAGTACTCGTGCTGTCCAACACGTAGTATGGTGTCGCACTCAGTGACTGAGAACCGTCATTCTTCAGG o D an GGCTTGACGTACGCCCCGTAAGCCAGACGCGTGTTCTCGCTCGACACCTTGGCAAAGCGTCCGGCCAGG an u CCAACCTGTGCTGCGAGTGTCGGGGTGAGGGCTCCCATTTCGCTAATGCCCGGTCCCACGTTAGGGTTA L n va GGATAAGAGCCTGAAACGCTGGCGATTGCCGTAGCTGTTGCCGTAGGTGTGTTTTGCGGGTTGTTCGAG an p Lu i gl ấ th t nai nh CTTCGCGAGGCGCCAGGATGTTGTAAGCCGTGCCGCCGTAGGGCTTGAAAGGAAGGACCTCTTTCGAG n tốn.Tới ă v ăn a m ệu CACCCCTTTTGATGTAAAAATACGTCGACCCCCATCATCAA ận v o n li lu ậnn.nDvă tài lu a ậ án >F8-HR an lu u đ L GGAATTCGGGGTACTGAGTTCGTGTTTCTGTCCTGGAGCTCGACGACGACGTTCATACCCGATCGCTCC n va n GAATTCAGAGTACCTGAGCACACACCCGTATCATGATAGAGCAGGTTGATAAAATTGTCCCTAAATCCG ua L u ATGTAATTCGGCCTGTTTGCCTTCATGACGCCGGTATTCACTTCTGAGTATTCGGTCGGCGGGGGAATA lie T AGGAGCGTGTCGGGATAGGTTAGCGAGGCGCTAAAGTCTTCGACTCCCATGACTCCGAGATATTCTGTG an Do GCATTGCTGTCCATGACCCAGTAAGGAGTGGGGTTAATCGATTGAGAACCGTCGTTCTTGAGCGGTTTA TACGGATTGCTCATTTGCCCCGAGATGGATGTCTTGCTTTCATCGTCCTCGATCCAGTTGTTAAAGAAGG sĩ iệ c h eut ACGTAGGCTCCGTAGGCTAGTCGCGTGTTATCGCTCGACACTTTCGCGAATCGGGCAGCTAATCCGACT TGCTCTGCCGACGTCTGGTCTAAGGCGCCCATCTCGCTGATGGCGGTACCGATATTGGGATCGGGATAA ACCCCTGAAAGAGCGGCGACGATCGCGGCAGTCTTGTCTTTAGCGCTCTGGACGTTTTCATAGGGAGTT GTCATCTGACCCGTGATGACTGTCTTGTTGGTACCGGTATCGATCCAATTGTTGAAAAACGCTTCTCGAG GAGCGAGGGGATTGTAAGCCGTGCCCCCGTACGGTTTAAAAGAAGGTCCTCTGTCTAGGACGCCCTTGA TGTCGAAATAGGTCGACCCCATATCACAA >F9-HR TGGGGGTTCCTGGTACAGAGTCGGTGTTTCTGGTCCTGCAGTTCTACGACCACGTTCATGCCGGACCGC TCGGAGTTCAGGGTCCCGGAGCACACGCCGGTATCGTGGTACAGGAGGTTGATGAAATTGTCACGGAA CCCGATGTAATTGGGTCTGTTGGCTTTCATCACCCCGGTATTGACCGTTCCGTACTCGATAGGAGGCGG GATCAGCAGACTGTCGGGATAAGTCAGGCTATCGGTAAAGTCCTCTACCCCCATCACACCCAAATATTT CGTGCTCCCGCTGTCCATGACGTAGTAAGGAGTTTGCACCAGAGACTGAGAACCGTCGTTCTTCAGGGG CTTGACGTACGCCCCGTAAGCCAGACGGGTGTTCTCGCTGGATACTTTCGCGAAGCGACCGGCTAGACC GACCTGAGCCGCTAGCGTGGTGCTGAGCGCCCCCATTTCGCTAATGCCCGGGCCCGAGTTGGGATTAGG 63 GTAGCTGCCGGAGACGCTCGCGACGACTGCGGCTGTTTCCGCTGCGGCCGTTTGAGTGGTATTCTCATA GGGGTTAGACATTTGCCCGGTGATGGTCGTCTTGTTGCCGTCTTCCGCGATCCATTTGTTAAAGAATGCT TCGCGAGGCGCCAGGATGTTGTAAGCCGTGCCGCCGTAGGGCTTGAAGGAAGGACCTCTGTCGAGCAC CCCTTTGATGTCAAAATAGTCGACCCCATAACAA >F10-HR CTGAGCTCGGTGTTCCGGTCGTGCAGCTCGAACCACCACGTTCATGCCCGAACGCTCCGAGTTGAGGGT GCCGGAGCACACGCCGGAATCGTGATACAGCAGGTTGATGAAGTTATCCCTGAACCCGATGTAGTTGG GCCTGAGCGCTTTCATGGCGCCGATGTTATCCTCGCCGTACTCCTCGGGAGGCGGCATGACTATAGTGT CCGGGTACGAGAGGCTGTTGGTGTAGTCCTCGACGGCCACCACTCCCAGGTAATTGGTGCCCGTGTTGT CCATGATCCAGTAAGGGGTCTGCGTGAGGGACTGGGAACCGTCGTTCTTGACGGGCTTTGACGTAGGCA CCGTAAGCGTAATTGTTGTTCTCACTTGGACAACTTGACCGAAΝCGACCCGGTGTTGGCGTTTTCTACCT GTCGCATAGGATTTATGCCTCCCCCTTGGTTGGGATTGGGΝAAGACGCCGGAAATCTTCATCACCTGCG AAACΝCTTCGTGTCTTTGGTGGTGTTCGTGTTGGTATAGACTTTGGACAGCTGACCGGAGGCGGACACG TTCTGCCCGGΝGATCCGTCTCCGCCTAGTTGCTAAACATGGACTCCTTGGAAΝCAAGΝGCGTCTGGAGG CCGTGCCGCAGTATGGCTTGAΝGCCACCGCCCTCGCTTCAAGGATTCCCCCTGATGTCGAACATACGTC TACACCCAAGACAAA o >F11-HR D an o tạ an TTGAATTCCTGGTGTCAGAGTTCGGTGTTTCGGTCCTGCAATTCCACCACCACGTTCATGCCTGACCGTT an u CGGAGTTGAGGGTCCCGGAGCAGACGCCGGTATCGTGGTACAGGAGGTTGATGAAATTGTCACGGAAC L n va CCGATGTAGTTGGGTCTGTTCGCTTTCATCACCCCGCTGTTAACCTCTCCGTACTCAGAAGGAGGGGGG an p Lu i gl ấ th t nai nh TGTGCGGTGGTGTCTAACACGTAGTAAGGCGTTGTTCCAAGTGACTGAGAGCCGTCGTCTTTTAGAGGC n tốn.Tới ă v ăn a m ệu TTCACATACGCTCCATAAGCCAGGCGCGTGTTCTCATTCGACACCTTGGCAAACCGACCGGCCAGACCG ận v o n li lu ậnn.nDvă tài lu a ậ án ACCTGTGCTGCTAGTGTCGGGGTGAGGGCTCCCATTTCGCTAATGGCCAGCCCCACGTTAGGGTTAGGA an lu u đ L TAAGAGCCTGAAACGCTGGCGATTGCTGCAGCTGTTGCCGTAGCTGTGTTTTGCGCCTCGTTCTTGTACG n va n GATTGGTCATTTGTCCCGTGATGGTTGTGTTGTTTCCATCGTCCTCGATCCAGTTGTTAAAGAAGGCTTC ua L u GCGAGGGGCCAGGGGGTTGTAAGCCGTGCCGCCGTAGGGCTTGAAGGAAGGACCTCTGTCGAGCACCC lie T CTTTGATGTCTAAATAGGTCGACCCCAAGTCAAT an Do >F12-HR sĩ iệ ATTAACAGACTGTCTGGGTAGGTAAGACTTTGCGTAAAGTCTTCTACCCCCATGACGCCCAAGTATTTC c h eut GGCAGCTCGACCACCACGTTCATGCCCGAACGCTCCGAGTTGAGAGGTGCCCGAGCACACGCCGGAGT CGTGATACAGCAGGTTAATGAAGTTATCCCTGAACCCGATGTAGTTGGGCCTGAGCGCACGCGTGGTGC CTATGTTATAATCGTCGTAGTCCTCGGGAGGCGGCACGACTATGGTATCCGGGTACGAGAGGCTGTTGG TGTAGTCCTCGACGGCCACCACTCCCAGGTAATTGGΝGCCCGTGTTATCCATGATCCAGAAGGGGGTCT GCGTGAGGGACTGGGAACCGTTTTACTTTGACGGGTTTGACGCGCT >F13-HR CTGTCTGGAGTTCGACGACCAACGTTCATGCCTGACCGTTCGGAATTGAGAGTACCTGAGCACAAACCC GTATCATGATAAAGCAAGTTTGAAAAATTGTCCCTAAATCCGATGTAATTTGGCCTGGTTGCCTTCATG ACCCΝGΝATTCACTTCTGAGTACTCGGACGGCGGGGGGATTAACAGACTGTCTGGATAGGTTAGACTG GGGCTAAAGTCTTCTACTCCCATGACTCCCAGATATTTTGGGGCATTGGTGTCCATGACCCAGTAAGGA GTGGGGTTAATTGACTGAGAACCGTCGTTTTTGAGAGGCTTCACATACGCTCCGTAGGCCAGGCGCGTG TTCTCATTCGACACCTTGGCAAACCGACCGGCTAACCCAACTTGCTCTGCTAGTGTCTGGTGAGGGCTC CCCATTTCGCTAAGGGCGGTACCAATTTTGGGATCGGATTAAACCCCGGAACGATCGGCAACGATCGCG CTGTTCTTGTCTTTATCGCTCTGGCCTCTTTCTTGTGGAGATGTCATCTGACCCCTGATGACTGTGTTGTG 64 GTTACAGCΝACCΝCTCCCCTTGTGGΝAAAAAACTTCTCGAGAGGCGAGGGGAΝTGTATGCCCTGCCCCC CCACAGTTΝAAAAAAAGAΝCΝTCTCTGTCGACACCCCTTTGATGAAATATCCCTCCCCCCC >F14-HR CGGTGTTCTGTCTGCAGTTCCACCACCACGTTCATGCCTGACCGTTCGGAGTTGAGGGTGCCGGAGCAC ACGCCGGTATCGGATTAAGGAGGTTGATGAAATTGCTCGGAACCCGATGTATTTGGGTCTGTTGGCTTT CATCACCCCGGTGTTGACCGCTCCGTACTCCGAAGGAGGGGGGATTAACAGACTGTCTGGATAGGTAA GACTGTGGCTAAAGTCTTCTACCCCCATGACGCCCAAGTATTTCGGGGCAGTGGTGTCCATTACCTAGT AAGGAGTGGGGTTAAGTGACTGAGAACCGTCGTTTTTCAGAGGCTTCACATACGCTCCGTAAGCCAGGC GCGTGTTCTCGTTCGACACTTTGGCAAACCGACCGGCCAGACCGACCTGTGCTGCTAGTGTCGGGGTGA GGGCTCCCATTTCGCTAATGGCCAGCCCCACGTTGGGGTTAGGATAAAAGCCTGAAACGCTGGCGATTG CTGCAGCTGTTGCCGTAΝCTGTGΝTTTGCGCCTCGTTCTTGTACGGATTGGTCATTTGTCCCGTGATGGT TGTGTTGTTTCCAGCCTCCTCGATCCAGTTGTAAAAGAAΝCΝTCGCGAGGGGCCAGGGTGTTGTAAGCC GTGCCGCCGTATGGCTAGAAGGAACGACCTCTGTCGAGCACCCCTTTGATGTCTAAATAGGCC n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 65 an Lu n va an Do an o tạ ΜỘT SỐ HÌΝH ẢΝH ΜIΝH HỌA TROΝG QUÁ TRÌΝH THỰC HIỆΝ ĐỀ TÀI é n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 66 an Lu n va an Do an o tạ n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 67 an Lu n va an Do an o tạ n Do a i Ta n u lie an Lu a p Lu sĩ hiệeut ạc g li ấ th ốt Tnaii nh n t vă ăn anmớ ệu o n v n li ậ lu ậnn.nDvă tài lu a ậ án n lu ua đồ L n va 68 an Lu n va an Do an o tạ