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DAI HOC QUOC GIA THANH PHO HO MINH NGUYEN THJ NHAT LE NGHIEN CLI’U XAC D!NH HAM LQNG SUDAN I TRONG T €1NG IT BANG PHLI€JNG PHAP VON AMPE TREN DIEN C C GLASSY CARBON Chuyén nganh Hoa Phan Tich Ma so 1[.]
DAI HOC QUOC GIA THANH PHO HO MINH NGUYEN THJ NHAT LE NGHIEN T €1NG CLI’U XAC D!NH HAM LQNG SUDAN I TRONG IT BANG PHLI€JNG PHAP VON-AMPE TREN DIEN C C GLASSY CARBON Chuyén nganh: Hoa Phan Tich Ma so: 1.04.03 LUAN VAN THAC SI KHOA HOC - HOA HUNG DAN KHOA HOC TS.NGUYEN PHLIC THANH THANH PHO HO CH I MINH — 2008 HOC UIc a m u n - iv Cack(Nieu×ie tit v Chid a n c i i c hinhv e vi Chu’dng 1:DAICURING VES L I D A N viii 1.1MCiD D U PHANTONG QUAN l 1.2PHAN LOAI,TINH CHATV A T L Y , H O A HOC .2 1.3D CC F H SPLY Chirfing2:CCJ PHAP CCPHOXUNG THUYETVACACPHLICING PHAPV O N HOAT A N PHAPXACD!NH A M P E pJ-IjyQNG p TRINH TICHGOPVAH O A TAN TOT RO N G Q U A PVIN - A M P E IONG 2.1.1PH PH/\ PCXCPHO 2.1.2PHLICJNG 2.1.3PHUCJNG 2.1.4CACYEU VIP H A N .7 2.1.4.1QUATRINHTICHGOP - 2.1.4.2QUATRINH HOA TA N 11 2.1.5.1DM“NNCLICGLASSY CHE“PHANNLON G CUASUDANI 13 PHAPK H A C D E MAU 2.2CACPHLICJNG XACD}NHPHAM 2.2.1PHLIONG CCARBON KHLI BO PHAPV O N TR SUDAN 14 AMPED I E N I 14 C 2.2.2 PHLIDNGP H A P SA CKYK H I DAU D O 2.2.3 PH LICJNGP H A P DIENDIMAOQUANV I I DAUD O UV 15 2.2.4 SAC KYL O N G H I E U N A N G C A O DAUD O Q U A N G HOA 15 2.2.5 SACKY L O N G H UNANGCAOVCI ID AU DOUV/Vis 16 2.2.6 PHL IONGP H A P SACKYL O N G G H E P KHO IPHO 17 PHANT H CNGHIE/M Chifdng3:KHAO SATQUI TRINHPHANTICH 18 1HOA C H A T , THIETBJ .18 3.1.IHOACHAT 18 3.2TOUUHOA 3.2.1KHAOOSATDIENC 50 19 CGLASSYCARBON 19 3.2.2KHAO S A T DOL A P LAI CUAABBEEMATDIENC C 21 3.2.4KHAO SATDUNGMOI 23 5pJ-JDJJNGDIII _ , .23 3.2.6THAIGIANTICHGOP .- 25 3.2.7 THETICH 3.2.8 TO“CCDDOOQUETTHE”VABIEN 3.2.9CACCHATG AYN H I E U - 27 3.2.9.1CAC C H A T C U N G H O S U D A N N H L I SUDAN I I , I I I , IV .27 3.2.9.2CAC CCHHAA“TTTTTAAAOOOMMAAUUCCOOTTRROONNGG TTLUICDJNNGGDD’’TT 227 3.2.10D U © NGCHUAN,GI NH IH A N P H A T H I E N , G I O ’IH A N D! L@N€i 28 3.2.10.1KHAOSETDLI’ONGCHUAN 28 3.2.10.2GUIHANPHAT HI E/N,GUIHANDINH LUNG 29 Chilling4 : PHANT I C H M A U T R O N G C i T TIN TH !TREfiNG 31 1C A C PHC1€JNGPHAPXLILYMAUTH CPHAMCNCHL’IASUDAN 314.1.1TRACINGHQPMAUDCINGIAN 31 4.1.2TRYINGHPMAUP H1’1CTAP 31 4.1.3XLILYMAUDEPHAN TICHBANGHPLC-UV/Vis 31 4.1.4XLLYMAUDEPHAN TICHBANGLCMS/MS 34 ° » 4.1.5XLLYM A U D EXAC DINHB A N G V O N - A M P E D U N G G C E 4.2 QUITRINHPHANTICH MAUTUCINGIT 35 4.4 MAUTLIONGC I T G I A HOA 37 4.5 MAUTUCJNGITHAUSANH 38 Chu'dng5: KETQUA THAOLUAN 43 47 TAIL IE/UT H A M K H A O Déh o é n thénhlua n gifxinbéythlongbié“ton ‘uons é u véiTSN guyénP h i t d c T h é n h , TSN guyénB é H o é i Anh,n h i t n g n g u é i Théykinh yé ud é l é n t i n h d i n d t , c h i ’ b a n , hiténgd i n e m t r o n g c a q u é t r i n h h p c t a p tr udng Dpih p c Khoah p c T i t nhient nh ’HoC h i Minh Xinchant h é n h dénhi ettinhtpom p i diéukic:n,giiipddchoemhoénthénhfuauviinnéy YincémonC é c Anh,C h i , CécBpnb triténgdpih o c , C é c bandongnghiép,Bpnb e thénhimvaGia dishdfif iifindpngv i é n , khichf,chiasé,gitipd é téihoént h d nhluanv én néy CACK Y AN API BDM E DHP VIET Acetonitril Ionho:ihoa hpc Dungdichd(mBrittonRobinsonDrencifc giptthuynganrcii Dihexadecylhydrogenphosphate DP Phifclngphii pcfcphoxungviphfin ECD Daud6di(nhoa EI EtH Phunion Etanol Daud o h u y n h q u a n g GCEGC/ MSHDME HPLC/CL HPLC/UV- Drencu’cglassycarbon PhiffingphiipsackykhighépkhoiphfiDie nc i f c giotthuyn g a n l r e o Sackyl o n g hieun a n g c a o diingdau d quanghoé VisMeOHLC S ac kyl o n g hieun a n g c a o diingdau doUV/Vis MS/MSLOD Metanol LOQMALD Sack$l o n g g h é p khoip h o IMFEMWC GidihanphiithienGi NTSCE c’lihandinhlifcing SPE Ionhoiiq u a n g hotd i fd’ié psuatthirdng Dienc f c phut h u y n g a n Vidi n c f c carbond a l o p D ienc u ' c Calomenb a o hoa Chiétphar a n CHIDAN CAC HINHVE Hinhl :l-Pickhii’cfiaSudanIquétthetir 0.0V—- V,tiChgop 90s,nongdQ Sudan15.l0" M,2-piCOxyhoiiSudanIquétthet’ir-1.0V—0.0V 14 Hinh2:Biéudiénc u ’ c l n g dpdongcuaSudanI0.2pg/rnL ._ 21 Ifinh3 : Phfid o c u a S u d a n I p g / m L , p H = , d BR E p a k '•436lTlV,d c › I T l photphatéE Peak-•260mV,demacetateE,. 202rriV 22 I-Finh4: Biéu diénsdichchuyénthetheopHcuaSudan I0.2pg/mL 24 I-dinh5 : Pic S u d a n I taic a c gi:it r | pH4 E = - m V , I= 19 8p A ; p H = m V , I = p A ; pH8E = - m V , I =1l.57qA 24 Ffinh6 : PicSudan I0.5mg/Lcicilc thcli g ia n tich gopl-tichgop 60s,2tich g p 90s,3-tich gdp s , 4-tichgop1 s , 5-tichgdp240sva - tichgop300s.2 Ifmh7 : Pic S u d a n 10.15mg/L tait h e t i c h g o p (1)-E=0.10V;(2)-E=0.00V;(3)- E=-0.l0V _ ,., „ _.2 IFinh8 : D u ’ c l n g c h u a n S u d a n I , t i c h g o p 180s;I -pi c S u d a n I m g / L ; p i c Sudan1 0.10mg/L; 3-p i c S u d a n 0.15mg/L;4 - p i c S u d a n I m g / L ; p i c SudanI0.25mg/L 28 Hinh9:DiffingchuanSudanI,thfiigian tichg6p180s,diencfcglassy carbon 29 Ifinh10:ScidokhoiquitrinhchiéttdchtiffingcitcotSPESilicage1 32 Hlnh11:Scidokhoiquitrlnhchiéttiichtu'c1ngf’itcotSPEC18 33 I-dinh1 : PicchuanSudanI,II,III,IVl 0 p g / L diingphifdngp h : i p LCM S/ MS.35 If mh : SudanI , I I , III, I V t h e m c h u a n l00pg/ Lt r é n n é n t u ’ f i n g c i t Chin S u p p LCMS/MS _ _ 36 Hinh14 : SackJdociiamautu’fing dtChinSutheoLCMS/MS 37 I-finh1 : Mautu’cingcitHauSanh(1),themchuanSudanI0.05mg/L(2),SudanI J.10mg/L(3)b a n g Von-ampedien cu'cGlassy lfmh16:Sack J doS u d a n I c h u a n m g / L , d i i n g i n : i yH P L C - U V / Vis,cot lfinh17: Sac k y doS u d a n I t h e m c h u a n m g/ L, d i i n g m i l y H P L C - U V / V i s , ctC18 .40 ITinh18:Sackydomautu’fingdt HauSanhdiingmiiyHPLC- UV/Vis .41 Hinh1 :pic(1)maut i t d n g dtRongVi(t,pic(2),(3),(4),(5),(6), (7)lfinlu’cIt t h e m c h u a n S u d a n I trénn é n t i f f i n g d t RongVi(tc i ciic nong d o s au 0.0473mg/L, l41mg/L,0.232mg/L,0.323mg/L,0.4l1mg/L,0.455mg/L .42 lfinh2 : s a c kyd o x a c dJ n h Sudan I trongc a c maut i f f i n g d t , pic(1)maut i f f i n g ft’tH(au Sanh,pic(2)mautu’fingft’t RongVi(t,pic(3)mautu’fingd’tChinSu.43