Khóa luận tốt nghiệp Viện đại học Mở Hà Nội LỞI CÁM ƠN Đe hồn thành khóa luận này, tơi xin chân thành căm ơn tiến sĩ Trằn Thị Như Hằng, thạc sĩ Hoàng Kim Chi hướng dẫn, chi bảo tận tình cho tơi suốt q trình nghiên cứu hồn thành khóa luận Cám ơn tồn the cán phịng Sinh học thực nghiệm-Viện hóa học hợp chất thiên nhiên tạo điều kiện giúp đỡ - -(Formatted: Vietnamese (Vietnam) thời gian thực tập phòng Xin càm ơn dạy bào, giúp đờ cúa thầy, cô khoa Công nghệ sinh hục Viện dại học Mớ Hà Nội trình học tập Tơi xin chân thành cám ơn gia đình bạn bè ln bên động viên tơi hồn thành khóa luận Xin chân than^^mW viện Đại học Nộ' Hà Nội, ngày 21 tháng 05 năm 2015 „ -f Formatted: Vietnamese (Vietnam) Sinh VÍCI\ Chu Thanh Hà Chu Thanh Hà KI8- 1104 Khóa luận tốt nghiệp Viện đại học Mở Hà Nội MỤC LỤC LỜI CẢM ƠN MỤC LỤC DANH MỤC CÁC CHỮ VIÉT TÂT DANH MỤC BÁNG, HÌNH VÊ, BIÊU ĐÒ MỚ ĐÀU I PHÀN I: TÔNG QUAN 1.1 Tổng quan nấm nội sinh 1.1.1 Sơ lược nam nội sinh 1.1.2 Các chất có hoạt tính sinh hợc từ nam nội sinh 1.1.3 Các nghiên cứu nấm nội sinh Việt Nam 1.2 Cây Trầm dó Aquilaria crassna nấm nội sinh Trầm dó 1.2.1 Sơ lược Trầm dó 1.2.2 Nghiên cuniive èơ thể tìội sirthi thtit bậỉ4rêh1bầổ'tẳh câytéhố^ùa ềhtìngJ |Yuan, J 1., Jian Nan B I Bing Y A N„ & Xu Dong, z (Ĩ006) Taxol producing fungi:—a—new—approach—to—industrial—production—of—taxolChinese—Journtd—oj Biotechnologv>22{\\ 1-61 L X _ ye mặt - {-Formatted: Font: 13 pt - - Comment [Kl]: IVtK ó đoạn nào? X i , di truyen VII lien hoa tong hợp-ta-xol-cuii vsv phun lập tư-eae-eay *■ ( Formatted: Font: 13 pt thêttg-dé-Tavt^-spp rat dáng ý: CÓ lẻ dã xãy-Fa-mộ( truyền gen theo* , ,1 c ~ ' TFormatted: Vietnamese (Vietnam) ' ~I chieu ngang (horizontal gene transfer) từ Taxus spp sang cac NNS cua chung] - • _ Các xanton I 'C {Formatted: Font: 13 pt 'ừ— < ' Í Formatted: Vietnamese (Vietnam) - X — \ Trên thuốc có tênCroton oblongifolius cùa Thái Lan, jPornpakakul^vh CI< >= - '.'Z T ■ '1 '?■ — Comment [K2]: DS cỏ sổ tài liệu mói hon Đẻ nghi update I j Formatted: Font: 13 pt I , , - { Formatted: Vietnamese (Vietnam) £2006) phân lập NNSEmericella variecolor có tống hợp bốn xanton ”\'ỊFormatted: Font: Not Italic Ị J I Formatted: Vietnamese (Vietnam) j CĨ hoạt tính kháng tế bào ung thư Bốn xanton là: samixanton (1), 14metoxytajixanton-25-axetat (2), tajixanton metanoat (3), tajixanton hydrat (4) Tất cã chất có hoạt tính kháng vừa phái chọn lọc khối u dày, khối u ruột kết, khối u vú Riêng chat số biểu thị hoạt tính gây độc trung bình tất Chu Thanh Hà KI8- 1104 !8.Ki£t L^ Cong, p^jul, (Thymelaeaceae) Formatted: Font color: Auto, French (France), Pattern: Clear I^tssỊgr, and M^i$cj_^yrlings "A new species of Aquilariav Vietnam." Blumea-Biodiversity, from Evolution Biogeography of Plants 50.1 (2005): 135-141J and yV Formatted: Font color: Auto, French (France), Pattern: Clear V Formatted: Font color: Auto, French (France), Pattern: Clear _ Formatted: Font color: Auto, French (France), Pattern: Clear _ _ million species estimate revisited Mvcological research 105(12) 1422-1432 8:20 Likhitayawuid K„ Angerhofer C.K (1993) Cytotoxic and antimalarial ii'S bisbenzylisoquinoline alkaloids from Sephania evecta, Jounal of Natural Products “ijv Formatted: Font color: Auto, French (France), Pattern: Clear _ _ »5, 56 (1) pp 30-38 Formatted: Normal, Indent: Left: 0.05", Numbered + Level: + Numbering Style: 1, 2, 3, + Start at: + Alignment: Left + Aligned at: 0" + Indent at: 0.25" Formatted: Font color: Auto, French (France), Pattern: Clear 9.-21 Mohamed R., Jong p L., & Zali M s (2010) Fungal diversity in wounded stems of Aquilaria malaccensis Fungal Diversity 43(1): 67-74 Formatted: Font color: Auto, French (France), Pattern: Clear Doi: Formatted: Font color: Auto, French (France), Pattern: Clear 10.1007/sl 3225-010-0039-z Formatted: Font color: Auto, French (France), Pattern: Clear 40:22 Mohanta J Tayung K and Mphapatral U.B (2008), “Antimicrobial potentials '(Formatted: Font color: Auto, Pattern: Clear of endophytic fungi inhabiting three Ethno-medicinal plants of Similipal Biosphere (Formatted: Font color: Auto, English (United States), Pattern: Clear Reserve, India", The Internet Journalof Microbiology 5(21 fill' Vk’l-I vli'll rJai hoc Moi 4-k23.Nimnoi, p., Pongsilp, N., & Lumyong s (2010) Genetic diversity and community of endophytic actinomycetes within the roots of Aquilaria crassna Pierre ex Lee assessed by Actinotnyceles-specific PCR and PCR-DGGE of I6S rRNA gene Biochemical Systematics and Ecology 38(4): 595-601 24 Formatted: Font color: Auto, English (United PornpakakulL _s.2 _ Lia_ngsakuj._ _J_.,_ Ngaiiiroianavanich, _ N\._ Roengsumran; _ s *v , -' States), Pattern: Clear Sihanonth p„ Piapukiew J- & Petsom A (2006) Cytotoxic activity of^fouj xanthones fromEmericella variecolor, an endophytic fungus isolated from Croton oblongifolius Archives of pharmacal research 29(2), 140-144À Formatted: Normal, Indent: Left: 0.05”, Numbered + Level: + Numbering Style: 1, 2, 3, + Start at: + Alignment: Left + Aligned at: 0" + Indent at: 0.25" Formatted: Font color: Auto, English (United States), Pattern: Clear 43r25.Sheila A., Wiseman, Douglas A Balentine, and Balz Frei "Antioxidants in tea."Critical Reviews in Food Science & Nutrition 37.8 (1997): 705-718 4-3:26 Skehan p., Storeng R., Scudicro D., Monks A., McMahon J., Vistica D., Warren J.T., Bokesch H., Kenney s., Boyd M.R., (1991), “New colorimetric cytotoxicity assay for anticancer agents”, Eur.J.Cance 27, pp 1162-1168 40 Chu Thanh Hà \ KI8- 1104 Formatted: Font color: Auto, English (United States) 27 ■jitrobcl G A.L E, Ford, JWqrapong i._ jC Harper, A MArifID, M .Grant, p c»ỵ ' w, Fung, and K Chan 2002 Ispoestacin an isobenzofuranone from Pestalotiopsis antifungal microspora,possessing Formatted: Normal, Indent: Left: 0.05", Numbered + Level: + Numbering Style: 1, 2, 3, + Start at: + Alignment: Left + Aligned at: 0” + Indent at: 0.25" antioxidant and Formatted: Font: 13 pt, Not Bold, Font color: Auto, Pattern: Pear activities Phytochemistry 60:179-183 Formatted: Font: 13 pt, Font color: Auto, Pattern: Clear 28.Strobel Gary, and Bryn Daisy (2003) Bioprospecting for microbial endophytes and their natural products Microbiology and Molecular Biology Reviews 67,4: 491 -502 Formatted: Font color: Auto, English (United I SBtes ) _ ■ _ I 29.Strobel Gary, et al "Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana." Microbiology 142.2 (1996): 435-440x , - -(Formatted: Font color: Auto, Pattern: Clear 44:311.Wang F w„ Hou z M., Wang c R Li p & Shi D H (2008) Bioactive metabolites from Penicillium sp., an endophytic fungus residing in Hopea hainanensis World Journal of Microbiology and Biotechnology, 24(10), 21432147 31 West, c P Izckor E Turner, K E & Eimi A A (1993) Endophyte effects on^x,- growth and perĩỉhblriòéllỏĩl tlll$&dwflhlafl£aVkitJrlsbDblWl gradient.Agronomy Journal 85(2), 264-270 Formatted: Font color: Auto, English (United States), Pattern: Clear Formatted: Indent: Left: 0.05", Numbered + Level: + Numbering style: 1, 2, 3, + start at: + Alignment: Left + Aligned at: 0" + Indent at: 0.25“ 32 Wu Chunwu, Caesar E Ordonez, and Chin-Tu Chen "F1P1: Fast 3-D PET reconstruction by Fourier inversion of rebinned plane integrals." Three- Dimensional Image Reconstruction in Radiology and Nuclear Medicine Springer Netherlands, 1996 277-296 4-5.-3'.Zich F.A and Compton J., 2001 The Final Frontier: Towards Sustainable Management of Papua New Guinea's Agarwood Resource TRAFFIC Occania.WWF South Pacific Programme Trang báo mạng http://thsngocphuong.blogspot.com/2012/05/tram-huong.html Field Code Changed jittp://www.tramhuong.info 41 Chu Thanh Hà KI8- 1104 ] http://www.tramhuoiiehanoi.coni.vn www.ncbi.nlm.nih.gov/BLAST http://www.zmt-bremen.de http://vietbao.vn/Suc-khoe/Tram-kv-nam-co-cong-dung-gi/45207693/248/ Thư viện Viện Đại học Mờ Hà Nội 42 Chu Thanh Hà KI8- 1104 PHỤ LỤC Hình ãnh chùng SHTr-Hc8 ni cấy điều kiện pH khác Hình ảnh sàng lọc hoạt tính sinh enzym ngoại bào dịch lên men A: Enzytn xenlulaza Chu Thanh Hà B: Enzym amilaza KI8- 1104 Formatted: Font: 13 pt, English (United States) Thư viện Viện Đại học Mờ Hà Nội Chu Thanh Hà KI8- 1104 Page 8: [1] Formatted Killer 5/15/2015 1:04:00 AM KIMCHI 5/14/2015 3:39:00 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Gần vào năm 201,0,4- tác giá Lê Huy Bá nghiên cứu đa dạng sinh học x cùa số thực vật thuộc họ Trầm khu hệ nấm nội sinh chúng Nghiên cứu chi ràng Trầm dó nguồn gốc khác (dựa 400 bp ADN) tạo trầm