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Tap chi Khoa hoc vd Cong ngh£ Nong nghiep Viet Nam - So 05(126)/2021 HOAT TINH GAY D C TE BAO UNG THU GAN VA UNG THU PHOI CUA CAO CHIET CAY LAN GAM TAI AN GIANG Nguyen Cong Kha1, Do Thj Hong Tucfi2, Nguyen Le Thanh Tuyen1 t m tat Nghien cdu ddpc thdc hien danh gia kha nang gay doc te bao ung thd gan va ung thd ph6 i cua cao chiet cay Lan gam dieu kien phong thi nghiem Cao chiet cay Lan gam ddpc thdc hien theo phddng phap ngam dam vdi dung moi va ndPc, ket hop song sieu am Te bao ung thii gan va ung thii phoi sd dung nghien cdu la te bao HepG2 va te bao A549 Hieu qua gay doc te bao ung thii gan va ung thii phoi ddpc xac dinh bang phiiOng phap MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromid)] PhiiOng phap MTT diia tren phan dng khd mau cua MTT- 3-(4,5- Dimethylthiazol-2 -yl)- 2,5- diphenyltetrazolium bromide, 1-tetrazol) co mau vang formazan co mau tim ty the cua te bao song Ket qua cho thay, cao chiet cay Lan gam bang niicfc va co kha nang gay doc te bao ung thii gan HepG2 va ung thii phoi A549 nhiing tac dung gay doc chiia cao Cay Lan gam til vimg That Son co kha nang gay doc te bao ung thii va la nguon nguyen lieu cho qua trinh san xuat cac san pham co kha nang ho trp va dieu tri benh tiiong lai Tii khoa: Lan gam, hoat tinh gay doc, ung thii gan, ung thii phoi I DAT VAN DE Cung vPi sd phat trien cua xa hoi, hien so ngdPi mac benh ung thii d Viet Nam cung nhii tren the gidi cang gia tang Benh ung thii da trd mot moi de doa cho sdc khoe cong dong, khong loai trii mot xa hoi Viec nghien cdu tim cac chat co kha nang dieu tri can benh ung thii, cung nhii ngan can sU phat trien cua cac te bao ung thii lam tang thdri gian song cho benh nhan luon diipc cac nha khoa hoc midtc va the gidi quan tam nghien cdu Den nay, da co nhieu hoat chat chong ung thii co nguon goc tii nhien da diipc kham pha va diia vao sd dung tren lam sang nhii paclitaxel, vinblastin va vincristin, camptothecin, (Nguyen Thi Ngoc Tram va ctv., 2011) Te bao ung thii gan HepG2 la mot dong te b io bat tit bao gom cac te bao ung thii bieu m gan ngiiPi, xuat phat tii mo gan cua mot nam gicfi da trang 15 tuoi Te bao ung thii phoi A549 la dong te bao ung thii kh i u d phoi va diipc phan lap tii phoi bi ung thii cua benh nhan nam 58 tuoi va thiiPng diipc dung nghien cdu ung thii va phat trien cac loai thuoc dieu tri ung thii Xu hiiPng tren the gidi va Viet Nam hien la nghien cdu sd dung cac san pham tiem nang td td nhien co kha nang gay doc cac te bao ling thu nhiing it tac dung phu, de tim va nguon cung cap phong phu Nhom Lan gam (Jewel orchid) gom co 04 loai khac nhd Anoectochilus spp., Goodyera spp., Ludisia spp vd Macodes spp (Hayden, 2016) Nhom Lan gam (Jewel orchid) la mot loai thao diipc quy hiem va chda nhieu hoat tinh sinh hoc nhii flavonoid, phenolic, polysaccharide va kinsenoside la hpp chat day tiem nang va day hda hen ho trp va dieu tri benh nhdng nam trd lai day cua cay Lan gam (Qi et al, 2018) Cac hpp chat cua cay Lan gam diipc sd dung de chong oxy hoa khuan, virus, benh tim mach, benh thoai hoa than kinh, ung thii va cac benh lien quan tPi lao hoa (Harleen et at, 2011); co tac dung chong ung thd, giam diiPng mau, ngan ngda thoai hoa te bao, giai doc cd the (Nguyen Van Binh va civ., 2018) va bao ve gan, chong tang md mau, chdng viem, bao ve mach mau va chong loang xddng An Giang la mot tinh thuoc vung Dong bang song Cdu long, diipc thien nhien ban tang co vdng That Son chda rat nhieu cay diipc lieu da dang va phong phu Theo dieu tra cua Trung tam Sam va Ddpc lieu Thanh Ho Chi Minh va Trung tam Cong nghe Sinh hoc An Giang thu thap ddpc khoang - giong cay Lan Gam tai viing Nui Cam, An Giang Cac giong Lan gam thu thap tai vung That Sdn, An Giang cho ket qua phan tich hinh thai hoc va sinh hoc phan td (vung trinh td ITS) thuoc loai Ludisia spp De gop phan nang cao gia tri ddpc lieu cay Lan gam, nghien cdu nham danh gia kha nang gay doc te bao ung thd gan va phoi, gop phan tao nguon nguyen lieu cho qua trinh san xuat cac san pham co kha nang ho trp va dieu tri benh II VAT LIEU VA PHl/ONG p h Ap NGHIEN CUtJ 2.1 Vat lieu nghien cdu Cay Lan gam (Lusidia discolor MK451745.1) thu thap tai xa Co To, huyen Tri Ton, tinh An Giang Dong te bao ung thd gan (te bao HepG2) va te bao ung thd phoi (te bao A549) ddpc cung cap td TrdPng Dai hoc Y Ddpc Thanh Ho Chi Minh ; Thing tam Cong nghe Sinh hoc tinh An Giang; 2TrdPng Dai hoc Y Ddpc Thanh Ho Chi Minh Tap chiKhoa hoc va Cong nghe Nong nghiep Viet Nam - So 05(126)/2021 2.2 Phtidng phap nghien cdu 2.2.1 Phtidngphdp too coo chief cay Lan gam Cay Lan gam (Lusidia discolor MK451745.1) thu thap tai xa Co To, huyen Tri Ton, tinh An Giang va liiu gid tai Trung tam Cong nghe Sinh hoc tinh An Giang (sau thang cham soc) Toan bo phan cua cay Lan gam (than, la va re) thu thap, rda sach va say kho d nhiet 50°C 96 gid va nghien hot 300 g hot Lan gam kho diidc chiet vcfi ethanol 80% va niidc cat theo ty le nguyen lieu va dung moi la 1:10 (w/v), ket hop vcfi song sieu am 12 gid, ngam 72 gid va de toi de tranh oxy hoa Sau 72 gid, hon hop diidc ly tam 5.000 vong/phut 20 phut, thu phan dich va bo phan ba Phan dich loc qua giay loc Whatman 0,45 pm, thu dich loc va tien hanh co quay chan khong d nhiet 50°C va dong kho bang may dong kho chan khong de thu cao chiet Lan gam Cao Lan gam niidc va khong bo sung them chat bao quan va diidc trfl d nhiet -4°C va thiic hien thl nghiem 2.2.2 Khdo sat hoot ttnh gay doc te b io ung thii phoi va ung thiigan in vitro Chuan bi mau thd: mau thd diiOc hoa tan DMSO cac dung dich me co nong 10 mg/mL Cac dung dich me diiOc bao quan d -20°C, dong va pha loang moi triidng nuoi cay de dat cac nong khao sat triidc xd ly te bao Tu an toan sinh hoc diigfc chieu UV 30 phut, cac dung cu diicfc hap tiet trung d nhiet 121°C (3-(4.5-diro«thy«hiazol-2-yl)-2.Sdiphenyltetrazolium bromide) 30 phut triidc si£ dung Tat ca hoa chat, dung cu deu phai diiOc xit triidc diia vao tu an toan sinh hoc Cac hoa chat, thuoc thd diiOc chuan bi tu an toan sinh hoc, loc vo trung qua mang loc 0,22 pm hoac chieu UV 60 phut triidc sd dung Te bao diiOc nuoi moi triidng MEM (te bao HepG2) hoac DMEM/F12 (te bao A549), bo sung 10% huyet bao thai be (FCS), mM L-glutamin, 100 IU/mL penicilin, 100 pM streptomycin va u d 37°C, 5% C binh nuoi cay Khi phu day binh nuoi cay cua te bao dat khoang 70 - 80%, thu va dem so liicfng te bao song, cho te bao vao dia nuoi cay 96 gieng vdi mat thlch hop U te bao d 37°C, 5% C - gid de te bao bam len be mat dia nuoi cay va phat trien on dinh Xd ly te bao vdfi cac mau thd d cac nong khac (100, 50, 25, 12,5 pg/mL) 72 gid, ndng cuoi cimg cua dung moi pha mau DMSO moi tnidng nuoi cay la 1% (v/v) Mau doi chdng am (moi tnidng nuoi cay chda DMSO 1%) diiOc tien hanh dong thdL Sau 72 gid, tien hanh danh gia ty le te bao song bang phiiong phap MTT Nguyen tac: Ty le song cua te bao diiOc xac dinh diia tren hoat tinh cua enzym succinat dehydrogenase (SDH) ty the cua te bao song Enzym chuyen MTT tinh the formazan co mau tim, tan dung moi hdu cof nhii isopropanoL Ty le te bao song ddOc tinh diia tren OD d 570 nm (Wan et al, 2011) «E,Z)-5-(4.5-dini8thyl»Mazot-2-yt)-1.3diphenylformszan) Hinh Ccf che cua phan dng cua phiiofng phap MTT Te bao sau xd ly vdi cac mau thd, diicfc loai bo moi triidng nuoi cay, bo sung moi triidng khong huyet chda 0,5 mg/mL thuoc thd MTT U te bao d 37°C, 5% C de tao cac tinh the formazan Sau gid, bo moi triidng chda MTT, tham kho, hoa tan tinh the formazan dung dich isopropanol acid hoa (HC1 0,1%), lac rung d nhiet phong den cac tinh the tan hoan toan, OD d biidc song 570 nm Tlnh ket qua: % dc che so vdi mau chdng = 100 - (OD mau thd/OD mau chdng) x 00 2.2.3 Phtidngphdp thong ke Cac so lieu thi nghiem xd ly bang phan mem Excel, thong ke bang phiidng phap Statgraphics plus 16.0 va trinh bay dang trung binh 2.3 Thdi gian va dia diem nghien cdu Nghien cdu diicfc thiic hien td thang dim thang 11 nam 2020 tad Khoa Diidc, Tnidng D p hgc Y Diidc Thanh Ho Chi Minh Tap ch! Khoa hoc va Cong nghe Nong nghiep Viet Nam -So 05(126)/2021 III KET QUA VA THAO LUAN 3.1 Dac diem hinh thai, phan bo, phan hoa hoc cac bo phan cua cay Lan gam Cay Lan gam (Lusidia discolor MK451745.1) thu thap tai xa Co To, huyen Tri Ton, tinh An Giang vdi cao khoang 500 - 614 m Cay Lan gam lia am va moc noi co bong ram tren cac tang da hoac tren mat dat Hinh thai cua than, re, la va hoa cua cac giong Lan gam thu thap tinh An Giang co nhflng dac diem nhvt sau: (i) Hoa co mau trang; co 05 canh hoa va nhuy co mau vang nhat; (ii) la co hinh dang oval hoac trai xoan; (iii) mau sac la co 03 mau khac nhii la co mau nau vdi cac diidng gan (03 - 05 gan) mau cam hoac do; la co mau xanh nhat vdi nhflng dfldng gan (03 - 05 dflcfng gan) mau vang va la co mau xanh dam vdi 03 - 05 gan la; la co be va be la bao boc quanh than Mau sac la thuoc va anh sang va am cua moi trfldng ma co sd khac biet; (iv) than co mau xanh trang, doi co mau nau do, thfldng nhan va khong phu long, than co mang nflcfc va diicfng kinh mm va chieu cao mm; (v) re diidc moc tii cac mau tren than cua cay, doi re cung diicfc hinh til than sinh (Hinh 2) i flavonoid va polysaccharide co tiem nang dieu tri ung thii gan Trong do, hop chat kinsenoside viia co tiem nang khang te bao ung thii phoi va viia co tac dung bao ve gan (Yu et a l, 2017) Bang Ket qua phan tich hoat chat sinh hoc cao chiet Lan gam Hoat cMt Ham liiOng hoat chat Ham liicfng phenolic 55,17 mg gallic acid/g Ham liidng flavonoid 724,58 mg quercetin/g Ham liicfng polysaccharide 93,25 mg GE/g Ham liidng kinsenoside 62,75 mg/g liidng kho 3.2 Ket qua tao cao chiet cay Lan gam Quy trin h trich cao chiet vcfi nguyen lieu la 300 gram (kho) Do am cua cay Lan gam la 81,19% (Bang 2) Hieu suat chiet cao niicfc va ethanol cua cay Lan gam dat lan liiqt la 6,62% va 5,06% Qua trinh tao cao chiet tii cac loai dung moi khac cho hieu suat trich ly khac nhau, nguyen nhan la neu sii dung niidc lam loai dung moi de ly trich thi mau trich nhiem nhieu tap chat nhii cac acid hiiu cd, diicfng va protein tan niicfc anh hiidng tofi qua trinh dinh tinh hay dinh liidng cac hop chat thien nhien Ngoai ra, neu sii dung tuyet doi se giam hieu suat chiet cao ethanol kho tham vao mau Vi vay, viec sii dung ethanol 80% lam dung moi se tao mot moi triidng toi liu cho viec li trich, tang sii tiep xuc mau va dung moi, tang hieu suat trich ly va bao quan mau khoi cac vi sinh vat (Bandar et al., 2013) Bang Ket qua phan tich am, hieu suat trich cao cua cay Lan gam Hinh Mau Lan gam Lusidia discolor MK451745.1 thu thap viing That Scfn, tinh An Giang Mau Lan gam thu thap tai xa Co To, huyen Tri Ton, tinh An Giang cho ket qua tiicfng dong vdi mau Lan gam Lusidia discolor MK451745.1 vcfi tiicfng dong 99,84% Cao chiet niicfc va ethanol cua cay Lan gam co chda cac hop chat co hoat tinh sinh hoc nhii alkaloid, flavonoid, saponin, steroid, terpenoid, tannin va phenol Tien hanh phan tich mot so phan hoat chat chinh cay Lan gam bang phiidng phap quang va HPLC cho thay, cay Lan gam lusidia discolor MK451745.1 co chda cac hoat chat co hoat tinh sinh hoc nhii phenolic (55,17 mg gallic acid/g), flavonoid (724,58 mg quercetin/g), polysaccharide (93,25 mg GE/g) va kinsenoside (62J3 mg/g trpng liicmg kho) (Bang 1) (Nguyen C e « Kha « rtv, 2019) Hop chat thuoc nhom ,A Chi tieu theo doi | Cao chiet niicfc Cao chiet ethanol Khoi liicfng mau tiicfi (g) 2.000 2.000 Do am (%) 80,19 80,19 Khoi liidng mau khd (g) 300 300 Khoi liidng cao kho (g) 19,87 15,18 Hieu suat chiet (%) 6,62 5,06 3.3 Khao sat hoat tin h gay doc te bao ung thii phoi in vitro Tac dung gay doc te bao ung thii phoi A549 cua cao thd Lan gam va Lan gam niicfc sau 72 gicf xii ly diicfc trinh bay bang Ket qua cho thay xd ly te bao 72 gid, cao thif Lan gam va Lan gam niidc the hien tac dong dc che tang triidng doi vdi te bao ung thii phoi A549 yeu, ty le dc che tang triidng te bao A549 d nong 100 pg/mL thap Tap chi Khoa hoc va Cong nghe Ndng nghiep Viet Nam - So 05(126)/2021 (< 10%) Cu the, cao chiet Lan gam bang cho hieu qua lie che te bao ung thu phoi A549 d nong 50 va 100 pg/mL cho hieu qua lie che dat lan lbpt la 7,14% va 2,73% Trong do, cao chiet Lan gam d nong 25 va 12,5 pg/mL khong cho hieu qua lie che te bao ung thii phoi A549 (Bang 3) Cao chiet Lan gam bang nbdc cho hieu qua lie che te bao ung thb phoi A549 nong 50 va 100 pg/mL dat hieu qua lan ldpt la 9,44% va 3,96% Trong do, cao chiet Lan gam bang nbdc d nong 25 pg/mL cho hieu qua lie che te bao ung thb phoi A549 dat 1,66% Cl nong cao chiet Lan gam bang nude 12,5 pg/mL khong co hieu qua gay doc te bao ung thii phdi A549 (Bang 3) Bang Tac dong cua cao Lan gam len ty le song cua te bao ung thii phdi A549 WbaoA549 Lan gam Trung binh Lan gam niidc Trung binh Ty le be che (%) 100 fig/ 50 fig/ 25 fig/ mL mL mL 6,59 -1,85 3,05 6,94 7,89 7,14 ± 0,67 2,18 2,95 2,73+ 0,48 9,08 9,35 3,78 3,23 9,89 9,44 + 0,41 4,86 3,96 ± 0,83 -1,69 -0,40 12,5 fig/mL -3,46 -4,60 -4,07 khao sat deu co kha nang gay doc te bao ung thu gan HepG2 nhiing tac dong gay doc chiia manh Cu the, cao chiet Lan gam bang cho hieu qua lie che te bao ung thii gan HepG2 d ndng 25, 50 va 100 pg/mL dat hieu qua Mn liipt la 14,70%; 14,84 va 14,71% va khong co sii khac biet gifla cac mbc ndng khao sat Trong do, cao chiet Lan gam d nong 12,5 pg/mL cho hieu qua be che te bao ung thii gan HepG2 thap hon chi dat 4,14% (Bang 4) Bang Tac dong cua cao Lan gam len ty Ie song cua te bao ung thii gan HepG2 Te bao HepG Lan gam Trung binh Lam gam nbdc Trung binh 1,53 -11,41 1,85 1,59 -10,73 -10,45 ± 0,17 1,66 Ket qua nghien ciiu cao chiet Lan gam niidc va co kha nang khang te bao ung thU phdi A549 Ket qua nghien ciiu thap hon so vdi ket qua nghien ciiu cua Le Dinh Chac va cong tac vien (2021), cao chiet Lan gam Anoectochilus setaceus Blume co chiia cac hoat chat quercetin, isorhamnetin ferulic acid va co kha nang khang te bao ung thii phdi A549 vdi gia tri IC 50 dat 14,85 pg/mL Hcfn niia, Yu va cong tac vien (2017) tinh sach hop chat polysaccharide tii cay Lan gam Anoectochilus roxburghii (wall.) Lindl co kha nang khang te bao ung thii phdi A549 vdi gia tri IC50 dat 15,12 pmol/mL (sau 48 gid) va gia tri IC50 dat 11,34 pmol/mL (sau 48 gid) 3.4 Khao sat hoat tinh gay doc te bao ung thii gan in vitro Tac dung cua cao thb Lan gam va Lan gam niidc sau 72 gid tiep xiic len ty le song cua te bao ung thii gan HepG2 diioc trinh bay bang O cac ndng cao chiet Lan gam bang va niidc Ty le be che (%) 100 fig/mL 50 fig/ mL 25 fig/ mL 12,5 fig/mL 14,48 13,93 14,07 3,28 15,16 15,16 15,29 3,65 14,48 15,42 14,75 5,50 14,71 ± 14,84 ± 14,70 + 0,39 0,80 0,61 4,14 ± 1,19 9,00 5,81 8,27 2,32 10,78 6,32 8,62 1,44 10,16 5,08 8,13 1,02 9,98 ± 0,90 5,74 ± 0,62 8,34 ± 0,25 1,59 ± 0,66 Cao chiet Lan gam bang niidc cho hieu qua be che te bao ung thii gan HepG2 d ndng 50 va 100 pg/mL dat hieu qua lan liidt la 5,74% va 9,98% Trong do, cao chiet Lan gam bang niidc d nong 25 pg/mL cho hieu qua be che te bao ung thb gan HepG2 dat 8,34% C1ndng cao chiet Lan gam bang nbdc la 12,5 pg/mL cho hieu qua gay doc te bao ung thii gan HepG2 thap hdn, chi dat 1,59% (Bang 4) Ket qua nghien ebu cao chiet lan gam nbdc va co kha nang khang te bao ung thb gan HepG2 Theo Yu va cong tac vien (2017), tinh sach hop chat polysaccharide tb cay Lan gam Anoectochilus roxburghii (wall.) Lindl co kha nang khang te bao ung thb phdi gan HepG2 vdi gia tri IC ^ dat 35,22 pmol/mL (sau 48 gid) va gia tri IC50 dat 33,67 pmol/mL (sau 48 gid) IV KET LUAN Cay Lan gam thu thap tai xa Co To, huyen I n Ton, tinh An Giang co dac diem hinh thai hoc n trinh tb gene tbdng dong giong Lan gam (Iiw iifa discolor MK451745.1) Cay Lan gam co ch b ac ach a* chat nhb phenolic (55,17 mg gallic add/g), Baammi (724,58 mg quercetin/g), pidysaccharide (9325egC B |Ji Tap chi Khoa hoc va Cong nghe Ndng nghiep Viet Nam - So 05(126)/2021 va kinsenoside (62,75 mg/g hieing kho) Do am cua cay Lan gam la 81,19%, hieu suat chiet cao niidc va ethanol cua cay Lan gam dat 6,62% va 5,06% Cao chiet Lan gam bang niidc nong 100 pg/mL cho hieu qua dc che te bao ung thii gan HepG2 va ung thii phoi A549 dat hieu qua 9,98% va 9,44% Cao chiet Lan gam bang d nong 50-100 pg/mL cho hieu qua dc che te bao ung thii gan HepG2 va ung thii phoi A549 dat hieu qua 14,84% va 7,14% LCfl CAM CfN Nhom tac gia chan cam On Trung tam Cong nghe sinh hoc An Giang va Trddng Dai hoc Y Ddpc Thanh Ho Chi Minh da tao dieu kien va ho trp nghien cdu t Ail ie u t h a m k h a o Nguyen Van Binh, Pham Thi Phiidng, Nguyen Ta Ldi, 2018 Nghien cdu mot so yeu to anh hiidng den qua trinh trich ly ham liidng polysaccharide toan phan nam linh chi Tap chi khoa hoc va Cong nghe, Dai hoc Thai nguyen, 180 (04): 3-8 Nguyen Cong Kha, Nguyen Pham Tuan, Lam Bao Nhii Phiidng, Nguyen Pham Tu, 2019 Phan tich ham liidng flavonoid, phenolic, polysaccharide va kinsenoside cua cay lan gam thu thap vung That sdn, An Giang va tinh Lam Dong Ky yeu Hoi nghi Cong nghe sinh hoc Toan quoc nam 2019 Nguyen Thi Ngoc Tram, Kamenarka Z., Bankova V., Popov S., Zvetkova E va Le Mai Hiidng, 2011 Hoat tinh gay doc te bao cua cac phan doan Alcoloid td cay Trinh nd hoang cung (Crium latifolium) Tap chi DUdc hoc, so 11: 21-23 Bandar, H., Hijazi, A., Rammal, H., Hach, A., Saad, Z and Badran, B 2013 Techniques for the extraction of bioactive compounds from Lebanese Urtica Dioica American J of Phytomedicine and C Therapeutics, 6: 507-513 Harleen, K.S., K Bimlesh, P Sunil, T Prashant, S Manoj, S Pardeep 2011 A Review of Phytochemistry and Pharmacology of Flavonoids Internationale pharmaceuticasciencia, 1(1) Hayden W.J., 2016 Jewels of the Orchidaceae Sempervirens Quarterly, Fall 2016: 6-7 Le Dinh Chac, Bui Bao Thinh, Nguyen Thi Yen 2021 Anti-cancer activity of dry extract of Anoectochilus setaceus Blume against BT474 breast cancer cell line and A549 lung cancer cell line Research J Pharm and Tech 14 (2): 730-734 Qi C.X., Q Zhou, Z Yuan, Z.W Luo, C Dai, H.C Zhu, 2018 Kinsenoside: A Promising Bioactive Compound from Anoectochilus Species Curent Medical Science, 38 (1): 11-18 Nguyen Van Ket, Hahn E.J., Park S.Y., Chakrabarty D., Paek K.Y., 2004 Micropropagation of an endangered orchid Anoectochilus formosanus Biologia Plantarum, 48(3): 339-344 Wan, C., Yanying, Y., Shouran, Z., Wei, L., Shuge, T and Shuwen, C„ 2011 Antioxidant activity and free radical-scavenging capacity of Gynura divaricate leaf extracts at different temperatures Pharmacognosy Magazine, (25): 40-45 Yu, X,, Lin, S., Zhang, J., Huang, L., Li, S., 2017 Purification of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl by high-speed counter current chromatography and its antitumor activity Journal of Separation Science, 40(22): 4338-4346 Cytotoxicity activity o f L u sid ia d isco lo r extract against lung and liver cancer cells in An Giang Nguyen Cong Kha, Do Thi Hong Tuoi, Nguyen Le Thanh Tuyen Abstract The study was conducted to evaluate the cytotoxicity of liver and lung cancer cells from Lusidia discolor extract under laboratory conditions Lusidia discolor extract were made by the method of soaking with alcohol and aqueous solvent combined with ultrasonic waves Liver cancer and lung cancer cells used in the study were HepG2 cells and A349 cells The cytotoxic effect of liver cancer cells and lung cancer cells was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromid)] method The method of MTT is based on the reduction of a yellow tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT) to purple formazan crystals by the mitochondria of cells The results showed that the Lusidia discolor extract with aqueous and alcohol has the ability to cause cytotoxicity of liver and lung cancer, but the toxic effect is not high The Lusidia discolor from That Son region having ability to cause cytotoxicity to cancer cells and can be used as a source of raw materials for the production of products capable of supporting and treating diseases in the future Keywords: Lusidia discolor, Cytotoxicity activity, lung cancer, liver cancer \g ? y nhan bah 28/4/2021 N g frp ian b ie n : 11/5/2021 Ngiidi phan bien: PGS.TS Nguyen Huy Sdn Ngay duyet dang: 04/6/2021 ... bao ung thii gan in vitro Tac dung cua cao thb Lan gam va Lan gam niidc sau 72 gid tiep xiic len ty le song cua te bao ung thii gan HepG2 diioc trinh bay bang O cac ndng cao chiet Lan gam bang... Trong do, cao chiet Lan gam d nong 12,5 pg/mL cho hieu qua be che te bao ung thii gan HepG2 thap hon chi dat 4,14% (Bang 4) Bang Tac dong cua cao Lan gam len ty Ie song cua te bao ung thii gan HepG2... tinh An Giang vdi cao khoang 500 - 614 m Cay Lan gam lia am va moc noi co bong ram tren cac tang da hoac tren mat dat Hinh thai cua than, re, la va hoa cua cac giong Lan gam thu thap tinh An Giang
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