(LUẬN văn THẠC sĩ) nghiên cứu sự lưu hành của virus gây bệnh thiếu máu truyền nhiễm ở gà (chicken infectious anemia virus CIAV) tại hà nội và vùng phụ cận

HỌC VIỆN NÔNG NGHIỆP VIỆT NAM NGUYỄN QUỐC DOANH NGHIÊN CỨU SỰ LƯU HÀNH CỦA VIRUS GÂY BỆNH THIẾU MÁU TRUYỀN NHIỄM Ở GÀ (CHICKEN INFECTIOUS ANEMIA VIRUS - CIAV) TẠI HÀ NỘI VÀ VÙNG PHỤ CẬN Ngành: Thú y Mã số: 60.64.01.01 Người hướng dẫn khoa học: PGS.TS Huỳnh Thị Mỹ Lệ TS Nguyễn Thị Thu Hằng NHÀ XUẤT BẢN ĐẠI HỌC NÔNG NGHIỆP - 2017 download by : skknchat@gmail.com LỜI CAM ĐOAN Tôi xin cam đoan cơng trình nghiên cứu riêng tơi, kết nghiên cứu trình bày luận văn trung thực, khách quan chưa dùng để bảo vệ lấy học vị Tôi xin cam đoan giúp đỡ cho việc thực luận văn cám ơn, thơng tin trích dẫn luận văn rõ nguồn gốc Hà Nội, ngày tháng năm2017 Tác giả luận văn Nguyễn Quốc Doanh i download by : skknchat@gmail.com LỜI CẢM ƠN Trong suốt thời gian học tập, nghiên cứu hoàn thành luận văn, nhận hướng dẫn, bảo tận tình thầy giáo, giúp đỡ, động viên bạn bè, đồng nghiệp gia đình Nhân dịp hồn thành luận văn, cho phép tơi bày tỏ lịng kính trọng biết ơn sâu sắc PGS.TS Huỳnh Thị Mỹ Lệ TS Nguyễn Thị Thu Hằng tận tình hướng dẫn, dành nhiều công sức, thời gian tạo điều kiện cho suốt trình học tập thực đề tài Tơi xin bày tỏ lịng biết ơn chân thành tới Ban Giám đốc, Ban Quản lý đào tạo, Bộ môn Vi sinh vật – Truyền nhiễm, Khoa Thú y - Học viện Nơng nghiệp Việt Nam tận tình giúp đỡ tơi q trình học tập, thực đề tài hồn thành luận văn Tơi xin chân thành cảm ơn tập thể lãnh đạo Đội Kiểm Dịch Động Vật Lưu Động – Chi Cục Thú Y Hà Nộiđã giúp đỡ tạo điều kiện cho suốt trình thực đề tài học tập Xin chân thành cảm ơn gia đình, người thân, bạn bè, đồng nghiệp tạo điều kiện thuận lợi giúp đỡ mặt, động viên khuyến khích tơi hồn thành luận văn./ Hà Nội, ngày tháng năm2017 Tác giả luận văn Nguyễn Quốc Doanh ii download by : skknchat@gmail.com MỤC LỤC Lời cam đoan i Lời cảm ơn ii Mục lục iii Danh mục chữ viết tắt v Danh mục bảng vi Danh mục hình vii Trích yếu luận văn viii Thesis abstract ix Phần Đặt vấn đề 1.1 Tính cấp thiết đề tài 1.2 Mục tiêu nghiên cứu 1.3 Ý nghĩa khoa học ý nghĩa thực tiễn đề tài Phần Tổng quan tài liệu 2.1 Tình hình nghiên cứu virus gây ức chế miễn dịch 2.2 Hiểu biết ciav bệnh ciav gây 2.2.1 Lịch sử địa dư bệnh .5 2.2.2 Căn bệnh 2.2.3 Đặc điểm dịch tễ học .7 2.2.4 Triệu chứng 11 2.2.5 Bệnh tích 13 2.2.6 Chẩn đoán bệnh 15 2.2.7 Biện pháp kiểm soát bệnh 18 2.2.8 Điều trị 19 Phần Vật liệu phương pháp nghiên cứu 20 3.1 Đối tượng nghiên cứu 20 3.2 Nội dung nghiên cứu 20 3.3 Địa điểm thời gian nghiên cứu 20 3.4 Vật liệu nghiên cứu 20 3.5 Phương pháp nghiên cứu 21 3.5.1 Phương pháp lấy mẫu 21 3.5.2 Phương pháp tách chiết ADN 21 iii download by : skknchat@gmail.com 3.5.3 Phương pháp tối ưu hóa phản ứng PCR chẩn đốn CIAV .22 3.5.4 Phương pháp nghiên cứu đặc điểm dịch tễ học phân tử 24 Phần Kết thảo luận 25 4.1 Kết xác định lưu hành ciav đàn gà nuôi hà nội vùng phụ cận 25 4.1.1 Kết tối ưu hóa phản ứng PCR để xác định CIAV 25 4.2.2 Sự lưu hành CIAV đàn gà nuôi Hà Nội vùng phụ cận 26 4.2.2 Kết PCR phát CIAV mẫu bệnh phẩm 29 4.2 Kết giải trình tự gen ciav 32 4.3 Kết phan tíchmột số đặc điểm dịch tễ học phân tử ciav 38 4.3.1 Kết phân loại genotype CIAV 38 4.3.2 Kết so sánh trình tự animo acid nhóm di truyền CIAV 39 4.3.3 Kết nghiên cứu trình biến đổi aminoacid protein VP1 41 Phần Kết luận đề nghị 43 5.1 Kết luận 43 5.2 Đề nghị 43 Tài liệu tham khảo 44 Phụ lục 49 iv download by : skknchat@gmail.com DANH MỤC CHỮ VIẾT TẮT Chữ viết tắt Nghĩa tiếng Việt ALV Avian Leucosis Virus ARV Avian Reovirus CAA Chicken Anemia Agent CAV Chicken Anemia Virus CIA Chicken Infectious Anemia CIAV Chicken Infectious Anemia Virus CPE Cytophathogenic Effect DNA Deoxyribonucleic acid ELISA Enzyme-Linked ImmunoSorbent Assay FAdV4 Fowl Adenovirus serotype IBDV Infectious Bursal Disease Virus IBDV infectious bural disease virus IBH inclusion body hepatitis IFN interferon IL-1 Interleukin-1 IL-2 Interleukin-2 MDCC Marek’s disease chicken cel MDV Marek’s Disease Virus PCR Deoxyribonucleic acid PCV Packed Cell Volume REV Reticuloendotheliosis Virus TCID50 50% Tissue Culture Infective Dose v download by : skknchat@gmail.com DANH MỤC BẢNG Bảng 3.1 Trình tự mồi đặc hiệu phát giải trình tự gen VP1 22 Bảng 3.2 Chu trình nhiệt phản ứng PCR 23 Bảng 3.3 Bố trí thí nghiệm nghiên cứu ảnh hưởng nồng độ mồi 23 Bảng 3.4 Trình tự gen tham chiếu 24 Bảng 4.1 Tổng hợp tình hình thu thập mẫu gà theo lứa tuổi 27 Bảng 4.2 Kết PCR phát CIAV mẫu bệnh phẩm 30 Bảng 4.3 Kết kiểm tra CIAV lứa tuổi gà 32 vi download by : skknchat@gmail.com DANH MỤC HÌNH Hình 2.1 Gà bệnh bị xuất huyết cánh, ngực; mào nhợt nhạt thiếu máu 12 Hình 2.2 Bệnh tích xuất huyết đặc trưng 14 Hình 2.3 Gan, thận nhạt màu 14 Hình 3.1 Sơ đồ vùng gen CIAV nhân lên mồi CAVVP3F/CAV2 22 Hình 4.1 Tối ưu hóa nhiệt độ bắt mồi phản ứng PCR 25 Hình 4.2 Tối ưu nồng độ mồi phản ứng PCR 26 Hình 4.3 Gà bị xuất huyết cánh, da, 27 Hình 4.4 Gan sưng, tụ huyết xuất huyết 28 Hình 4.5 Thận sưng to, nhạt màu 28 Hình 4.6 Tuyến ức bị teo, xuất huyết 29 Hình 4.7 Minh họa kết phản ứng PCR phát CIAV 29 Hình 4.8 Kết PCR giải trình tự gencủa chủng phân lập C20 33 Hình 4.9 Trình tự gen 26 chủng CIAV từ vị trí 1- 110 33 Hình 4.10 Trình tự gen 26 chủng CIAV từ vị trí 111- 440 34 Hình 4.11 Trình tự gen 26 chủng CIAV từ vị trí 141- 609 35 Hình 4.12 Kết so sánh mức tương đồng nucleotide 26 chủng CIAV 37 Hình 4.13 Cây phát sinh chủng loại CIAV 38 Hình 4.14 Trình tự amino acid protein VP1 CIAV 40 Hình 4.15 Trình tự amino acid protein VP1 CIAV 40 Hình 4.16 Sự thay đổi amino acid VP1 nút dẫn tới nhánh CIAV lưu hành Việt Nam 42 vii download by : skknchat@gmail.com TRÍCH YẾU LUẬN VĂN Tên tác giả: Nguyễn Quốc Doanh Tên Luận văn: “Nghiên cứu lưu hành virus gây bệnh thiếu máu truyền nhiễm gà (Chicken Infectious Anemia Virus - CIAV) Hà Nội vùng phụ cận” Ngành: Thú y Mã số: 60.64.01.01 Tên sở đào tạo: Học viện Nơng nghiệp Việt Nam Mục đích nghiên cứu: - Xác định có hay khơng có lưu hành CIAV đàn gà thuộc địa bàn nghiên cứu - Nghiên cứu số đặc điểm dịch tễ học phân tử CIAV Nội dung nghiên cứu Trong khuôn khổ nghiên cứu đề tài, thực nội dung sau: - Xác định lưu hành CIAV đàn gà nuôi Hà Nội vùng phụ cận - Giải trình tự gen mã hóa protein VP1 CIAV - Phân tích số đặc điểm dịch tễ học phân tử CIAV Phương pháp: Phương pháp lấy mẫu; Phương pháp tách chiết ADN; Phương pháp tối ưu hóa phản ứng PCR chẩn đốn CIAV; Phương pháp nghiên cứu đặc điểm dịch tễ học phân tử Kết kết luận: (1) Trong số 14 trại kiểm tra có đến 13 trại (tỷ lệ 92,86%) phát có lưu hành CIAV; 01 trại (tỷ lệ 7,14%) âm tính với kết PCR phát CIAV Trong tổng số 124 mẫu bệnh phẩm gà có triệu chứng lâm sàng kiểm tra tỷ lệ phát CIAV cao (63 mẫu dương tính, tỷ lệ 50,81%) (2) Đã giải trình tự gen thành cơng phân đoạn gen VP1 CIAV 26 chủng CIAV lựa chọn để giải trình tự có mức tương đồng cao trình tự nucleotide, từ 96,8% đến 100% (3) Qua phân tích đặc điểm dịch tễ học phân tử dựa vào trình tự gen mã hóa protein VP1, kết luận: - Các chủng CIAV lưu hành có đa dạng tương đối mặt di truyền - Các chủng nằm nhánh di truyền khác với chủng virus vacxin viii download by : skknchat@gmail.com THESIS ABSTRACT Name of Master student: Nguyen Quoc Doanh Thesis title: “Researchthe prevalence of Chicken Infectious Anemia Virus - CIAVof chicken in Hanoi and surrounding areas” Major:Veterinary Medicine Code:60.64.01.01 Name of Institute: Vietnam National University of Agriculture Research Objectives Determiningthe prevalence of CIAVof chicken in Hanoi and surrounding areas Researching molecular epidemiological characteristics of CIAV Research contents: - Determining the prevalence of CIAVof the chicken in Hanoi and surrounding areas - Sequencingprotein VP1 geneof CIAV - Researching molecular epidemiological characteristics of CIAV Methodology - Sampling method; - DNA extraction method; - PCR for identifying CIAV; - Methods for researching molecular epidemiological characteristics of CIAV Results: (1) By using PCR,13 farms (92.86%)of tested 14 farms were CIAV positive; there was only one farm (7.14%) was CIAVnegative; In total124 samples of chickenwhich had clinical symptoms,there were 63 CIAV positive samples (50.81%); (2) 26 sequencing of VP1gene of CIAVin this study sharedfrom 96.8% to 100% nucleotide identity (3) By phylogenetic analysis based on nucleotide sequences of VP1 gene of CIAV showed that: - CIAV strains in this study have geneticvariation; - These strains were located in different branches of phylogenetic tree with vaccine virus strains ix download by : skknchat@gmail.com ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGAAAGTCCCCCCCTTTAAAAAAGGGGGG >C10_VP3F CCCCCCCGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACAC ATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGATCCCCTTTCACCCAAATGGGTTTTAA >C10_CAV2 CCGGGGGGGACTTAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTT GCAGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCC CATCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAG TTCTGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTC ACGTATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCC CCGTACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAG ATGATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGG GCGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTT ATATCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGT CTGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTG GTAATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAG GCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGC AGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGA TACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCG TTGATGGTCCGGGTGGAGTATCTTCTTGGAAACGTCCCCATCTTAAAAATGGGGGGG >C11_VP3F CCCCCGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGAGAG ATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCCC ACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGAC CGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGCTAA AAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGAT 55 download by : skknchat@gmail.com GGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACCTCA AGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAGG AAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATGACT ATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGGGGC TATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCTAGC GTCAATGAACCTGACATACGTGAGCAAAATCGGAGGACCCATCGCCGGTGAGTTGATTGCGGACGGGT CTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCGA CACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTAATC ACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACATTG AAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGAGCGCCTTTCCCCCCCAAAAAAAATTGTTTGTGAT TA >C11_CAV2 TGGGGGGAACTAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACG TATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCG TACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG ATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCG GGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATA TCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTG CGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTA ATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCT TGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAG ATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATA CCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTT GATGGTCCGGGTGGAGTATCTTCTTGAGACGCCCCCCCTCTAAAAAAAGGGAAAGAA >C12_VP3F CCCCCCCGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT TGAAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGTACCCCTTCTTCCCAAAAATTAAAATATTAA >C12_CAV2 CGGGGGGACTAACGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT 56 download by : skknchat@gmail.com ATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGGAGACTCCCCCCTCTTTAAAAAAGGGGGGGGG >C13_VP3F CCCCCGGAACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT TGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGTACCCCTTTCACCCACAATATTTTTTCTTA >C13_CAV2 CCGGAAAGGACTGAGCGGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCT TGCAGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCC CATCTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAG TTCTCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTC ACGTATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCC CCGTACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAG ATGACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGG CGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTA TATCTTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTC TGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGG TAATCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAG GCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGC AGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGA TACCAATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACGGCCCGTGAAACTTGTTGGGTGGGCCTGA AACACCGTTTGATGGGCCCGGGTGGGAGTATCTTCTTGGAGAGGGCCCCATCTTTTAAAAAAAAACAA AAA >C14_VP3F ACAATCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAG AGAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGC 57 download by : skknchat@gmail.com TCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGGTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCC GCGACACCATCAGCATGGTGGGAGATGGGGCCTTAATGATGATGCAGCCCACGGGACTCTTGGCCGAT TCTTTTAATCACCCCTAAGCCAAATGAACCCTGCAAGAACATGGGGTCGCCATGTTTTGGGGGGCTGGG CACCTGTTTCCGAACACATTGGAAACCCGGCTTTTCAGCTTCCCTTGCCCACTAAAGAATGAGGATGCC CTTTCTAGCCCACAAAATGGAAAAAAG >C14_CAV2 GCCGCTCGCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT ATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACC AATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACGGGCCGTGAAACTTGTTGGGTGGGCCTGAAACA CCGTTTAATGGTCCCGGGTGGAAGTATCTTTCTTGGAAAGGTCCTCCCTTTTTAAAAAAACGCCCGCT >C15_VP3F ACACCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAGA GATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCC CACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGA CCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCTA AAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGA TGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCTC AAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAG GAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGAC TATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGGG CTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTAG CGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGGG TCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCG ACACCATCAGCATGGTGGGAGATGGGCCCTTAATGATGATGCAGCCCCACGGAACTCTTGCCCAATTCT TTAATCACCCCTAAGCCAAATGAACCCTGCAAGGACATGGGGTCGCATGTTTTGGGGGGCTGGGCACCT GTTTCCAACACATTGAAAACCCGCTTTTCAGCTTCCTTGGCCACTAAGAATGAGGATACCCTTTTTAGCC CAAAAATTTTTATTATAT >C15_CAV2 58 download by : skknchat@gmail.com ATAGATGAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACG TATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCG TACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG ACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGGAGACGCCCCTTCTTTTAAAAAAGGGGTGGG >C16_VP3F ACAGCCGGGACATCACGGTGTACAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGATACCCCTCTCCCCACAATTGTTTGGTC TC >C16_CAV2 ACCAACGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT ATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGTACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGAAAGTCCCCACCCTTTAAAAAATGTTTTGGT 59 download by : skknchat@gmail.com >C17_VP3F AAACCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTA ATCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGTACCCTCTTTACCCACAAAGCCAATA TAAAAAA >C17_CAV2 AGAGCGCCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGAC TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCCGGATCTCTCTGCAGTGAAGGGGTTTCCAACGGCCCGTGAAACTTGTTGGGTGGCCTGAACACCG TTTGATGGTCCCGGTGGGAGTATCTTCTTGGAGACGCCCCCCCCTTTTAAAAAAATTTTATTGGC >C18_VP3F CCAACCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAA 60 download by : skknchat@gmail.com TCACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTTCAGCTTCCTTGCCACTAAGAAGAGGAGACCCTTCTTCCCCACAAGGGAAA TGAAG >C18_CAV2 AGGTCGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGAC TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATTAGAGTGATTGTAATTTCCAGCGATA CCAATCCCGGATCTCTTCTGCAGTGAGGGGTTTTCCAACGGCCCGTGAAACTTGTTTGGTGGGCCTGAA ACACCGTTTGATGGGTCCCGGGTGGGAGTATCCTTCTTGGAGAGTTCCCTCCTCCTTTAAAAAAAAACT CTTCCCT >C19_VP3F CCACCGGTACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGG GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGC GACACCATCAGCATGGTGGGAGATGGGGCCTTAATGATGATGCAGCCCCACGAACTCTTGCCCAATTCT TTAATCACCCCTAAGCCAAATGAACCCTGCCAGGACATGGGGTCGCATGTTTGGGGGGCTGGGCACCTG TTTCCGACACATTGAAACCCCGCTTTTCAGCTTCCTTGGCCACTAAGAATGAGGAGACCCTTTTTAGCCC AAAATTTTTTATTTTGGGTAAGGCGTCATAAGTTTTTCTAATTTTGTAATGTGTGGTG >C19_CAV2 ACGGGCGGCTGAAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGC AGGGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCAT CTCCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTC TCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACG TATGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCG TACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATG ACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA 61 download by : skknchat@gmail.com TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGGTGGCCTGAACACCGTT GAAGGTCCGGGGGGGAGTATCTTCTTGAGACGCCCCCCCCTTTAAAAAAATCCTGTTG >C20_VP3F ACAACCGGAATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAGA GATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTCC CACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGGA CCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCTA AAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAGA TGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCTC AAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTAG GAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGACTGCCGAACCCCCAATCTACTATGAC TATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACAGCGGGGGG CTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTAG CGTCAATGAACCTAACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGGG TCTAAATCACAAGCCGCGGAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCGCG ACACCATCAGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGATTCTTTAAT CACCCTAAGCAAATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGACAC ATTGAAAACCCGCTTTTCAGCTCCTTTGCCACTAAGAATGAGATACCCTTTCTCCCCACAAAAGGCGGG GGCG >C20_CAV2 ACGCGAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCAG GGTCATTTGCTTAGGGTGATTAAAGAATCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATCT CCACCATGCTGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCTC CGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGTA TGTTAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGTA CATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGAC TCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGTCTCACACTATACGTACCGGGGCGGG GGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATCT TCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGAAACGCCCCCCCCTTTAAAAAAGGGGGGGTA >C21_VP3F GCCCCCGGGAATCACGGTGTTCAGGACACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAGAG AGATCCGGATTGGTATCGCTGGAATTATAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCTC CCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAGG ACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGCT AAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAAG ATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACCT CAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTTA GGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATGA CTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGGG 62 download by : skknchat@gmail.com GCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCTA GCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACGG GTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCGC GACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTAA TCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCGACACAT TGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGATCCCCTTCAGCCAGATTTCTTCTTTGGAAAC GTTCCATCTTTAATTATGGTTT >C21_CAV2 AAAGGGAACTAAGCGGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTG CAGGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCA TCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTT CTCCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCAC GTATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCC GTACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGAT GACTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGC GGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTAT ATCTTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCT GCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGT AATCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGG CTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCA GATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTATAATTCCAGCGAT ACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGT TGATGGTCCGGGTGGAGTATCTTCTTGGAGACGTCCCCTCCTTAAAAAGGGGTTTTAGTT >C22_VP3F AAAACCGGGTACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCCGTTGGAAACCCCTCACTGCAG AGAGATCCGGATTGGTATCGCTGGAATTATAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGC TCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAGCGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACCACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGAGTCATCTTTCTCACGGAAGGACTCATTTTGCCTAAAAACAGCACAGCGGGG GGCTATGCAGACCACATGTACGGGGCGAGAGTCGCCAAGATCTCTGTGAACCTGAAGGAGTTCCTGCT AGCGTCAATGAACCTCACATACGTGAGCAAACTCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTAAATCACAAGCCGCGGAGAACTGGCCTAACTGCTGGCTACCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAAATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACAC ATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGATGAGGAGCCCCTTTCACCCACAATTATTGATACTC >C22_CAV2 ACGGACAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATTTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGTAGCCAGCAGTTAGGCCAGTTCT CCGCGGCTTGTGATTTAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGAGTTTGCTCACGT ATGTGAGGTTCATTGACGCTAGCAGGAACTCCTTCAGGTTCACAGAGATCTTGGCGACTCTCGCCCCGT ACATGTGGTCTGCATAGCCCCCCGCTGTGCTGTTTTTAGGCAAAATGAGTCCTTCCGTGAGAAAGATGA CTCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC 63 download by : skknchat@gmail.com TTCGTCGAAGTCGCTTGAGGTGGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGC GAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAA TCAAGCTTTCTTTTAGCTCGCTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTT GGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGA TCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTATAATTCCAGCGATAC CAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACGGCCGTGAACTTGTTGGTGGCCTGAACACCGTTG ATGGTCCGGGTGGAGTATCTTCTTGGAGACGCCCCCCCTCTTAAAAAAGGGGGGTG >C23_VP3F CCACCCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAAGATGGGCCTTAATGATGATGCAGCCCCACGGACTCTTGCCGGTTCTT TAATCACCCTAAAGCAGATGACCCTGCAAGAACATGGGGTCGAATGTTTGGGGGGCTGGGCACCTGGT TCCAACACATTGGAAACCCGGCTTTTCAGCTTCCTTGGCCACTAAGAAATGAGGGAATCCCTTTCAGCC ACAATTTGTTTTTTT >C23_CAV2 AGTATTAACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGATCGCTTCTTCGAGGGAGGCTTGG GTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAATCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATCT TAGCGTGGGAGCGCGAAGCATTCGCGCAGCCACACAGCGATAGAGTTGATTTGTAATTCCAGCGATAC TAATCCGGGATCTCTCTTGCAGTGATGGGTTTCCAATGCCGTGGACTTGTTGGTGGGCTTGAAC >C24_VP3F ACAACCGGGACATCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT 64 download by : skknchat@gmail.com AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAGATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTCCGACA CATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGAAACCCTTTTACCCACAAGGGGAAGAGG G >C24_CAV2 GCCAAGGACTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAACACCGTTGAT GGTCCGGGTGGAGTATCTTCTTGAAAACGCCCCCACACTTAAAAATATCGGGTAA >C25_VP3F CAAACCCGGGACTCACGGTGTTCAGGCCACCAACAAGTTCACGGCAGTTGGAAACCCCTCACTGCAGA GAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGCTCGCGCT CCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGACTTGAG GACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAAACGAGC TAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGACTGTAA GATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGCACAACC TCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGAGCTTTT AGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCTACTATG ACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACCGCGGGG GGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTTCCTGCT AGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGCGGACG GGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCCCTCCG CGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTTCTTTA ATCACCCTAAGCAGATGACCCTGCAAGACATGGGGTCGCATGTTTGGGGGGCTGGCACCTGTTTCCGAC ACATTGAAAACCCGCTTTTCAGCTTCCTTGCCACTAAGAATGAGGAAACCCCTTTTAGCCCACAAATTTT TAATTAAA >C25_CAV2 GCCAATGGCTGAGCGGGTTTCATGTGTCGGACAGGTGCCAGCCCCCAAACATGCGACCCATGTCTTGCA GGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAGGCCCATC TCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGCCAGTTCT GCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTGCTCACGT ATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTCGCCCCGT 65 download by : skknchat@gmail.com ACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGAAAGATGA TGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACCGGGGCGG GGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAATTTATATC TTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTCGGTCTGCG AGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTAGTGGTAAT CAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGGGAGGCTTG GGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAGTTGCAGATC TTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCAGCGATACCA ATCCCGGATCTCTCTGCAGTGAGGGGGTTTCCAACTGCCCGTGAACTTGTTGGGGGGCCTGAAACACCG TTTGATGGTCCGGGGTGGGAGTATCTTCTTGGAGACGTCCCCCATTCTTAAAAAATTCATAATT >C26_VP3F GGGGGCCAGAAATACTGGTACATTTGTCATGGTCCCAGTGATCTTTCGTGTGAGAGAGCGTCCTCCTCA CTGCAGAGAGATCCGGATTGGTATCGCTGGAATTACAATCACTCTATCGCTGTGTGGCTGCGCGAATGC TCGCGCTCCCACGCTAAGATCTGCAACTGCGGACAATTCAGAAAGCACTGGTTTCAAGAATGTGCCGGA CTTGAGGACCGATCAACCCAAGCCTCCCTCGAAGAAGCGATCCTGCGACCCCTCCGAGTACAGGGTAA ACGAGCTAAAAGAAAGCTTGATTACCACTACTCCCAGCCGACCCCGAACCGCAAGAAGGTGTATAAGA CTGTAAGATGGCAAGACGAGCTCGCAGACCGAGAGGCCGATTTTACGCCTTCAGAAGAGGACGGTGGC ACAACCTCAAGCGACTTCGACGAAGATATAAATTTCGACATCGGAGGAGACAGCGGTATCGTAGACGA GCTTTTAGGAAGGCCTTTCACAACCCCCGCCCCGGTACGTATAGTGTGAGGCTGCCGAACCCCCAATCT ACTATGACTATCCGCTTCCAAGGCATCATCTTTCTCACGGAAGGACTCATTCTGCCTAAAAACAGCACC GCGGGGGGCTATGCAGACCACTTGTACGGGGCGAGAGTCGCCAAGATCTCCGTGAACCTGAAAGAGTT CCTGCTAGCGTCAATGAACCTGACATACGTGAGCAAAATCGGAGGCCCCATCGCCGGTGAGTTGATTGC GGACGGGTCTCAATCTCAAGCCGCGCAGAACTGGCCTAATTGCTGGCTGCCGCTAGATAATAACGTGCC CTCCGCGACACCATCGGCATGGTGGAGATGGGCCTTAATGATGATGCAGCCCACGGACTCTTGCCGGTT CTTTAATCACCCTAAGCAGATGACCCTGCAAGACATGGGTCGCATGTTTGGGGGCTGGCACCTGTTCCG ACACATTGAAACCCGCTTTCAGCTCCTTGCCACTAAGAATGAGGGATCCCTTCAGCCAATTTATCTCAT >C26_CAV2 TAGGGAATTTTTAATTTGGGGTGAGCAATAATCTTAGCAAGGTGCCAGCCCCCAAACATGCGACCCATG TCTTGCAGGGTCATCTGCTTAGGGTGATTAAAGAACCGGCAAGAGTCCGTGGGCTGCATCATCATTAAG GCCCATCTCCACCATGCCGATGGTGTCGCGGAGGGCACGTTATTATCTAGCGGCAGCCAGCAATTAGGC CAGTTCTGCGCGGCTTGAGATTGAGACCCGTCCGCAATCAACTCACCGGCGATGGGGCCTCCGATTTTG CTCACGTATGTCAGGTTCATTGACGCTAGCAGGAACTCTTTCAGGTTCACGGAGATCTTGGCGACTCTC GCCCCGTACAAGTGGTCTGCATAGCCCCCCGCGGTGCTGTTTTTAGGCAGAATGAGTCCTTCCGTGAGA AAGATGATGCCTTGGAAGCGGATAGTCATAGTAGATTGGGGGTTCGGCAGCCTCACACTATACGTACC GGGGCGGGGGTTGTGAAAGGCCTTCCTAAAAGCTCGTCTACGATACCGCTGTCTCCTCCGATGTCGAAA TTTATATCTTCGTCGAAGTCGCTTGAGGTTGTGCCACCGTCCTCTTCTGAAGGCGTAAAATCGGCCTCTC GGTCTGCGAGCTCGTCTTGCCATCTTACAGTCTTATACACCTTCTTGCGGTTCGGGGTCGGCTGGGAGTA GTGGTAATCAAGCTTTCTTTTAGCTCGTTTACCCTGTACTCGGAGGGGTCGCAGGATCGCTTCTTCGAGG GAGGCTTGGGTTGATCGGTCCTCAAGTCCGGCACATTCTTGAAACCAGTGCTTTCTGAATTGTCCGCAG TTGCAGATCTTAGCGTGGGAGCGCGAGCATTCGCGCAGCCACACAGCGATAGAGTGATTGTAATTCCA GCGATACCAATCCGGATCTCTCTGCAGTGAGGGGTTTCCAACTGCCGTGAACTTGTTGGTGGCCTGAAC ACCGTTGATGGTCCGGGTGGAGTATCTTCTTGGAGAACGTCCCATCTTAAAAATATTT 66 download by : skknchat@gmail.com MỘT SỐ HÌNH ẢNH MINH HỌA TRONG QUÁ TRÌNH THỰC HIỆN ĐỀ TÀI 67 download by : skknchat@gmail.com 68 download by : skknchat@gmail.com 69 download by : skknchat@gmail.com ... máu truyền nhiễm gà (Chicken Infectious Anemia virus- CIAV). Chúng thực đề tài ? ?Nghiên cứu lưu hành virus gây bệnh thiếu máu truyền nhiễm gà (Chicken Infectious Anemia Virus - CIAV) Hà Nội vùng phụ. .. Luận văn: ? ?Nghiên cứu lưu hành virus gây bệnh thiếu máu truyền nhiễm gà (Chicken Infectious Anemia Virus - CIAV) Hà Nội vùng phụ cận? ?? Ngành: Thú y Mã số: 60.64.01.01 Tên sở đào tạo: Học viện Nông... BIẾT VỀ CIAV VÀ BỆNH DO CIAV GÂY RA Bệnh thiếu máu truyền nhiễm gà (Chicken Infectious Anemia - CIA) ghi nhận bệnh gà loại virus gây Đặc trưng bệnh virus tác động gây tượng thiếu máu tủy xương