Na+, k+ ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity

... declare that the PhD thesis entitled Na+, K+- ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity is no more than 100,000 words in length, exclusive of tables, ... isoforms in single skeletal muscle fibres to have a more complete understanding of the underlying molecular properties and adaptations of...

Ngày tải lên: 04/12/2015, 13:59

... applied and (c) assume a similar role by putative endogenous ouabain or OLF Ouabain- sensitive and -insensitive a- isoforms of Na+/K+-ATPase in rats All isoforms of the catalytic a- peptide of Na+/K+-ATPase ... rat by another group in collaboration with Hamlyn [21] by means of two independent assays, a radioimmunoassay using an anti -ouabain Ig and an enzymat...

Ngày tải lên: 23/03/2014, 17:21

... Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+/K+ ATPase and H+/K+ ATPase in the range of the binding site ... and its position in the binding site was optimized Results Effects of truncation of the cytoplasmic H4-H5 loop of the a subunit of N...

Ngày tải lên: 19/02/2014, 16:20

... 675 Stimulation of pNPPase activity of Na+ ⁄ K+-ATPase H Homareda and M Ushimaru Table Phosphorylation of Na+ ⁄ K+-ATPase from pNPP, Pi and ATP For phosphorylation by pNPP or Pi, Na+ ⁄ K+-ATPase ... Stimulation of pNPPase activity of Na+ ⁄ K+-ATPase Fig Na+- dependent activation curves for Na+ ⁄ K+-ATPase and pNPPase in the pres...

Ngày tải lên: 07/03/2014, 16:20

... interactions between skeletal muscle TnC( 1–91) and TnI( 98–182), we have constructed two single chain chimeras composed of these domains The chimeras were formed by residues 1–91 of TnC and residues ... the TnC( 1–91) and TnI( 98–182) form a regulatory subunit, and that the first half of the C terminus of TnI is important for this binding Structural inf...

Ngày tải lên: 16/03/2014, 18:20

... on the 86Rb uptake into red cell ghosts after h incubation at 37 °C, and the effect of the molecular chaperone a-crystallin (1 mgÆmL-1) resealed inside red cell ghosts upon the rubidium uptake ... ouabain, 50 mM sucrose and 50 mM fructose on the 86Rb uptake into red cell ghosts after h incubation at 37 °C, and the effect of the molecular chaper...

Ngày tải lên: 17/03/2014, 03:20

... effect on the palytoxin- induced current across the apical membrane of colon epithelial cells Ouabain inhibition of the colonic H+/K+-ATPase has been a matter of controversy in many investigations ... Palytoxin Palytoxin + borate Palytoxin Palytoxin + ouabain Palytoxin + vanadate Palytoxin + scilliroside Palytoxin, Na ± free Basolateral depolarization (apical Na+) B...

Ngày tải lên: 17/03/2014, 11:20

... mM NADH, lgámL)1 of pyruvate kinase, and 2.5 lgámL)1 LDH in a total volume of mL The reaction was initiated by an addition of 50 ng of Na+/K+-ATPase Na+-dependent ATPase activity was also measured ... be con®rmed that the Na+/K+-ATPase activity, as determined by the coupled assay system, increased linearly with an increase in the incubation time and enzyme concentrati...

Ngày tải lên: 24/03/2014, 00:21

... the past three decades, has clearly shown that in addition to its established role as an ion pump, Na+/K+-ATPase also functions as a signal transducer to relay messages from the plasma membrane ... J., Haas, M., Shapiro, J.I., Askari, A & Xie, Z (2000) Ouabain interaction with cardiac Na+ +-ATPase /K initiates signal cascades independent of changes in intracellular Na+ and...

Ngày tải lên: 24/03/2014, 00:21

... aztecus, Penaeus duorarum, Penaeus setiferus, Penaeus stylirostris Áp suất thẩm thấu nhóm có độ đẳng áp với môi trường nước biển 745 mOsm/Kg, 768 mOsm/K g, 680 mOsm/Kg 699 mOsm/Kg Áp suất thẩm thấu ... trường có độ mặn thấp điều cho thấy tôm thẻ chân trắng điều hòa tình trạng ASTT dịch máu cao s ng môi trường có độ mặn thấp Áp suất thẩm th...

Ngày tải lên: 02/04/2014, 10:20

... 3.3 Hoạt tính men Na/ K ATPase mang tôm thẻ chân trắng nghiệm thức có độ mặn thấp khác Hoạt tính men Na/ K ATPase mang tôm chuyển vào môi trường có độ mặn thấp trình bày Hình 4; hoạt tính men giảm ... P=0,05 thấp KẾT QUẢ THÍ NGHIỆM 3.1 Áp suất thẩm thấu dịch máu tôm thẻ chân trắng độ mặn thấp khác Áp suất thẩm thấu...

Ngày tải lên: 22/07/2014, 11:21

... received general anaesthesia with ketamine/xylazine 80:10 mg/kg via intraperitoneal injection Sepsis was induced by cecal ligation and perforation (CLP) An incision was made along the linea alba The ... tissues in the cardiovascular system, including cardiomyocytes, vascular smooth muscle, and endothelial cells, and has been shown to mediate anti-apoptotic, anti-inflammatory, and...

Ngày tải lên: 13/08/2014, 03:21

... substitution mutant snake DNases I The thermal stabilities of the wild-type and mutant enzymes of the snakes were examined by measuring the activities remaining after incubation for 40 at various ... mechanism of generating a thermally stable enzyme form from a thermally unstable one: frog, toad and newt DNases I all have a Ser205 insertion in a domai...

Ngày tải lên: 20/02/2014, 23:20