molecular analysis of na k atpase

... 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase in the range of the binding ... K. , Kaya, S., Abe, K & Mardh, S (2001) The oligomeric nature of Na+ /K+ -transport ATPase J Biochem (Tokyo) 129, 335342 13 Karlish, S.J.D (1980) Characterization of conformational changes in (Na, K) -ATPase ... R.W (2000) Contribution of quarternary protein interactions to the mechanism of energy transduction in Na+ /K+ -ATPase In Na/ K- ATPase and Related ATPases (Taniguchi, K & Kaya, S., eds), pp 349356...

Ngày tải lên: 19/02/2014, 16:20

... oligomeric nature of the enzyme J Biochem (Tokyo) 116, 1360–1369 ˚ Taniguchi K, Kaya S, Abe K & Mardh S (2001) The oligomeric nature of Na ⁄ K- transport ATPase J Biochem (Tokyo) 129, 335–342 Abe K, Kaya ... ions with Na+ ⁄ K+ -ATPase IV Affinity change for K+ and Na+ of Na+ ,K+ -ATPase in the cycle of the ATP hydrolysis reaction J Biochem (Tokyo) 109, 70–77 17 Arato-Oshima T, Matsui H, Wakizaka A & Homareda ... inactivated ATPase On the other hand, omission of NaCl completely inactivated ATPase (Fig 9B) 677 Stimulation of pNPPase activity of Na+ ⁄ K+ -ATPase Fig Na+ -dependent activation curves for Na+ ...

Ngày tải lên: 07/03/2014, 16:20

... ouabain-insensitive isoform of Na+ /K+ -ATPase In most tissues, the Na+ /K+ -ATPase is a heterodimer consisting of a 112 kDa hydrolytic a-peptide, through which the vectorial transport of Na+ and K+ takes place, ... -insensitive a-isoforms of Na+ /K+ -ATPase in rats All isoforms of the catalytic a-peptide of Na+ /K+ -ATPase have similar, high affinity for ouabain [17] with Kd in the order of one or a few nanomolar [12,17] ... in Na, K- ATPase isoform expression in rat cardiac ventricle J Biol Chem 266, 9327–9331 26 Hansen, O (2000) Real concentrations of isoforms of Na, KATPase from western blots of tissue homogenates...

Ngày tải lên: 23/03/2014, 17:21

... protection of the membrane enzyme Na/ K- ATPase from inactivation by the heat shock protein a-crystallin Na/ K- ATPase activity decreased upon incubation with fructose, H2O2 and methylglyoxal However, Na/ K- ATPase ... specific inhibitor of Na/ K- ATPase, caused a 35% decrease in the 86Rb uptake within the ghost cells This demonstrates that the ghosts have functional Na/ KATPase The presence of a-crystallin within ... efficiency of the Na/ K- ATPase Resealing of the molecular chaperone a-crystallin within a red cell ghost, followed by stress from post-translational modifications protected the ghost cell Na/ K- ATPase...

Ngày tải lên: 17/03/2014, 03:20

... forms Effects of pressure on ¯uorescence probes Fig E€ect of pressure on the intensity and kmax of intrinsic ¯uorescence of membrane-bound Na+ /K+ -ATPase (A) and solubilized Na+ / K+ -ATPase (B) Ten ... thickness and cross-sectional area of the lipid bilayer of the present pig kidney Na+ /K+ ATPase is dif®cult because the precise lipid composition is unknown Generally, phospholipids of pig kidney ... domain of Na+ , K+ -ATPase: The tetrameric nature of the enzyme J Biol Chem 273, 24339±24345 41 Tsuda, T., Kaya, S., Yokoyama, T., Hayashi, Y & Taniguchi, K (1998) Half site modi®cation of Lys-480 of...

Ngày tải lên: 24/03/2014, 00:21

... functions of the NKA isoforms in skeletal muscle NKA Function isoform α1 Key housekeeping isoform, large contribution to Na+ /K+ exchange mainly during basal conditions α2 Key α isoform in skeletal muscle, ... of these isoforms include global knockout models, which are not successful in helping determine functions of each isoform, as a knockout of either of the three α isoforms leads to the death of ... phosphorylation via protein kinase A had no effect on the K+ affinity of α1/β1isoforms Activation by PKA phosphorylation alone of PLM influences the apparent Na+ affinity of the Na+ , K+ -ATPase α1/β1 and...

Ngày tải lên: 04/12/2015, 13:59

... activation of p42/44 MAPK (also called ERK1/2) through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation of this ... Role of protein kinase C in the signal pathways that link Na+ / K+ -ATPase to ERK1/2 J Biol Chem 276, 42050–42056 32 Tian, J., Gong, X & Xie, Z (2001) Signal-transducing function of Na+ -K+ -ATPase ... 8851– 8854 Yamamoto, K. , Ikeda, U., Seino, Y., Tsuruya, Y., Oguchi, A., Okada, K. , Ishikawa, S.-e., Saito, T., Kawakami, K. , Hara, Y & Shimada, K (1993) Regulation of Na, K- adenosine triphosphatase...

Ngày tải lên: 24/03/2014, 00:21

... binding region of the C2B domain of synaptotagmin J Biol Chem 273, 32966–32972 Nakatani Y, Tanioka T, Sunaga S, Murakami M & Kudo I (2000) Identification of a cellular protein that functionally interacts ... mRNA was purified using the mRNA Purification Kit 5794 (Amersham Biosciences) Immature pistil mRNA was used in the construction of a kTriplEx cDNA library as follows A TimeSaver cDNA Synthesis Kit ... PCRamplify internal fragments of the cDNA The nature of the fragments was confirmed by DNA sequencing and by comparison with the known partial amino-acid sequences Specific internal primers were...

Ngày tải lên: 30/03/2014, 11:20

... loài Penaeus aztecus, Penaeus duorarum, Penaeus setiferus, Penaeus stylirostris Áp suất thẩm thấu nhóm có độ đẳng áp với môi trường nước biển 745 mOsm/Kg, 768 mOsm /K g, 680 mOsm/Kg 699 mOsm/Kg Áp ... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro 1998 ... thải nước khỏi thể Cơ chế vận chuyển ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland)...

Ngày tải lên: 02/04/2014, 10:20

... volume The analysis of gene expression represents a key area of research in the study of human cancer and a number of chapters in Molecular Analysis of Cancer address this subject Global RNA expression ... Institutes of Health, Bethesda, MD KAZUMA OHYASHIKI First Department of Internal Medicine, Tokyo Medical University, Tokyo, Japan JUNKO H OHYASHIKI First Department of Internal Medicine, Tokyo Medical ... Institute of Molecular Medicine, Oxford, UK TOSHIKI UCHIDA First Department of Internal Medicine, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan IGOR VORECHOVSKY Department of Biosciences...

Ngày tải lên: 08/04/2014, 12:51

... http://www.translational-medicine.com/content/7/1/48 a a ERK2 p21 pERK p16 pAKT p27 AKT D1 C 10 20 75 120 Rb b ERKs AcH3 Days C ERKs b Days C TRAIL FAS Flip-L c pRb Flip-S p53 ERKs Days C ERKs C c d pBAD ER-alpha BCL2 ERKs ERKs Days C Figure ... determination of ERK2, pERK, Akt and pAkt, 35 μg of total protein extracts were separated on a 12% polyacrylamide gel and blotted Antibodies used were: ERK2 (Santa Cruz sc-154, dilution 1:500), pERK ... combination of ATRA μM and BPA, at the indicated concentrations for 48 hrs (B) Cell cycle analysis and apoptosis in K5 62 cells after 48 hrs of treatment with 60 and 100 μM BPA (C) Cell cycle analysis...

Ngày tải lên: 18/06/2014, 15:20

... loài Penaeus aztecus, Penaeus duorarum, Penaeus setiferus, Penaeus stylirostris Áp suất thẩm thấu nhóm có độ đẳng áp với môi trường nước biển 745 mOsm/Kg, 768 mOsm /K g, 680 mOsm/Kg 699 mOsm/Kg Áp ... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro 1998 ... thải nước khỏi thể Cơ chế vận chuyển ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland)...

Ngày tải lên: 22/07/2014, 11:21

Ngày tải lên: 07/08/2014, 17:22

... 52-wk exposure Furthermore, all Molecular analysis of hprt mutation 383 Table DNA sequence analysis of hprt mutant in splenic cells of B6C3F1 male mice in 52-wk study Type of mutation Number of ... as the source of total RNA for RT-PCR reactions For the initial RT-PCR amplification of hprt mRNA, µl of the cell lysate was used in a final volume of 20 µl RT-PCR containing µl of 10×VM buffer ... selection and DNA sequence analysis of numerous mutant clones An important component in the application of lymphocyte hprt assays for the study of in vivo mutation is the characterization of DNA sequence...

Ngày tải lên: 07/08/2014, 18:20

... Pourkarim MR, Amini-Bavil-Olyaee S, Verbeeck J, Lemey P, Zeller M, Rahman M, Maes P, Nevens F, Van Ranst M: Molecular evolutionary analysis and mutational pattern of full-length genomes of hepatitis ... JE, Norkrans G, Horal P: Genotyping of hepatitis B virus by restriction pattern analysis of a pre-S amplicon J Virol Methods 1998, 72(2):163-74 Kato H, Ruzibakiev R, Yuldasheva N, Hegay T, Kurbanov ... 14(1):3-16 Serin MS, Akkiz H, Abayli B, Oksuz M, Aslan G, Emekdas G: Genotyping of hepatitis B virus isolated from chronic hepatitis B patients in the south of Turkey by DNA cycle-sequencing method...

Ngày tải lên: 12/08/2014, 02:20

... deletion of ARP6 Last but not least, this study investigates the T-DNA tracking in the yeast cell, showing that knockout of ARP6 influences AMT process not by increasing uptake of T-DNA but by weakening ... of knowledge of the molecular principles of T-DNA transfer process AMT is one of the most powerful genetic techniques for generation of transgenic plants Transgenic plants have a great many of ... transport of molecular cargoes becomes a prime target for the viral usurpation (Ouko et al., 2010) A great many of techniques were adopted to analyze the exploitation of MTs by different kinds of viruses,...

Ngày tải lên: 09/09/2015, 10:08

... MAPK cascade Two complete MAPK cascades, MEKK1/MKKKs-MKK4/5/9-MPK3/6 and MEKK1-MKK1/2-MPK4, are involved in signaling transduction of MAMP induced primary response (Tena et al 2011) Activation of ... Jinghan and Guo Song Finally, I gratefully acknowledge the financial support provided by National University of Singapore I TABLE OF CONTENTS ACKNOWLEDGEMENTS I TABLE OF CONTENTS ... targeting of T-strand, including a kinase CAK2Ms, which indirectly help target VirD2 to the transcriptionally active regions through phosphorylation of the largest subunit of RNA polymerase II (Bako...

Ngày tải lên: 09/09/2015, 11:19

... T-DNA extraction and analysis Fig 3.7 Border analysis of S pombe transformants Fig 3.8 Comparison of S pombe binary vectors Fig 3.9 Comparison of S pombe and binary vectors Fig 3.10 Affect of ... Preparation of A tumefaciens genomic DNA 2.2.5 Preparation of S pombe genomic DNA 2.2.6 Preperation of S cerevisiae genomic DNA 2.3 Analysis of potential integrant into the S pombe genome 2.4 Total RNA ... HRP horseradish peroxidase SDS sodium dodecyl sulphate kb kilobase(s) or 1000bp sec second kDa kilodalton(s) ssDNA single-stranded DNA km kanamycin UV ultraviolet M molar v/v volume per volume...

Ngày tải lên: 12/09/2015, 08:20

... serum albumin kb kilobase(s) or 1000 bp C- terminal carboxyl terminal kDa kilodalton(s) C cytidine Km kanamycin Cb Carbenicillin lacZ β-galactosidase gene DMSO dimethylsulfoxide M molar DNA deoxyribonucleic ... One dimensional SDS PAGE of A6007 and A6340 membrane proteins 137 Fig 4-9 138 Generation of pEX18TcKmUFDF Fig 4-10 Result of knockout: colony and represent the successful Atu4026 knockout strain ... life Finally, I thank the National University of Singapore for awarding me a research scholarship to carry out this interesting project i TABLE OF CONTENTS ACKNOWLEDGEMENTS I LIST OF PUBLICATIONS...

Ngày tải lên: 13/09/2015, 20:52

... encodes a single-stranded DNA-binding protein Proc Natl Acad Sci USA 85: 2909-2913 De Costa, D M., Suzuki, K and Yoshida, K 2003 Structural and functional analysis 153 of a putative gene cluster ... PCR analysis Subsequetly, the mutants were also subjected to Southern analysis to confirm the deletion of Atu4026 (data not shown) We named the Atu4026 deletion strain as A4026 M P 3kb 1kb Figure ... Deschenes, S., Baker, S and Liskay, R 1999 Mammalian DNA mistach repair Annu Rev Genet 33: 533-564 Bundock, P., Den Dulk-Ras, A., Beijersbergen, A and Hooykaas, P J J 1995 Trans-kindom T-DNA transfer...

Ngày tải lên: 13/09/2015, 20:53