localization of na k atpase in photoreceptors

... cascade of events Based mainly on observations with rat myocytes, additional roles in signal-transducing function of ouabain’s interaction with Na+ /K+ -ATPase were attributed to tyrosine protein kinase, ... elicited by the interaction of a minor fraction of ouabainaltered Na+ /K+ -ATPase with neighbouring proteins (Src, growth factor receptors and Ras), i.e by protein–protein interaction In myocytes, ... ouabain-insensitive isoform of Na+ /K+ -ATPase In most tissues, the Na+ /K+ -ATPase is a heterodimer consisting of a 112 kDa hydrolytic a-peptide, through which the vectorial transport of Na+ and K+ takes place, and...

Ngày tải lên: 23/03/2014, 17:21

... functions of the NKA isoforms in skeletal muscle NKA Function isoform α1 Key housekeeping isoform, large contribution to Na+ /K+ exchange mainly during basal conditions α2 Key α isoform in skeletal ... effects of a longitudinal training program on NKA isoform abundance in human are unknown Therefore Chapter investigated whether 12 weeks of high-intensity interval training (HIT) in aged individuals ... 16% (McKenna et al., 1993) No changes in muscle [3H]ouabain binding or NKA isoform abundance were seen in elite cyclists completing additional interval training involving x at 80% of peak power...

Ngày tải lên: 04/12/2015, 13:59

... Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase in the range of the binding ... nucleotide binding sites in Na, K- ATPase In Na/ KATPase and Related ATPases (Taniguchi, K & Kaya, S., eds), pp 115122 Elsevier Science, Amsterdam ể FEBS 2004 59 Linnertz, H., Miksik, I., Kvasnicka, P., ... loop protein of Na+ /K+ ATPase [55] In conclusion, neither method revealed any indication of a conformational change of the N-domain upon ligand binding in the investigated GST fusion protein It is...

Ngày tải lên: 19/02/2014, 16:20

... affinity of Na+ ⁄ K+ -ATPase for Na+ [14] The resultant increase in Na+ binding was regarded as Na+ occlusion In the absence of oligomycin, NaCl gradually inhibited pNPPase activity and Rb+ binding ... responsible for oligomycin-induced occlusion of Na+ within Na ⁄ K- ATPase J Biol Chem 271, 25604–25610 18 Homareda H, Ishii T & Takeyasu K (2000) Binding domain of oligomycin on Na+ ⁄ K+ -ATPase Eur J Pharmacol ... for K+ [38] Increasing the KCl concentration in the presence of NaCl and oligomycin induces the activation curve with two phases (Fig 3) As K+ binding competes with Na+ binding [15], it is likely...

Ngày tải lên: 07/03/2014, 16:20

... subunit of Na+ /K+ ATPase in HEK293 cells [24,25] In Sf9 cells, however, expression of H+ /K+ -ATPase a subunit without a corresponding b subunit produces a ouabain-insensitive H+ /K+ ATPase [26] Finally, ... (Fig 2) The inhibition of palytoxin-induced currents by vanadate, a known blocker of apical H+ /K+ -ATPase [22], suggests that this enzyme is involved in the formation of the palytoxininduced channels ... latter findings, as the palytoxin-induced conductance was insensitive to mM ouabain while being inhibited at low concentrations of orthovanadate Similar findings showing a palytoxin-induced K+ efflux...

Ngày tải lên: 17/03/2014, 11:20

... irreversible tritium incorporation into Na+ /K+ -ATPase are shown in Fig Tritium incor- Fig E€ect of pressure on tritium incorporation into membrane-bound Na+ /K+ -ATPase Membrane-bound Na+ /K+ -ATPase was mixed ... emission maximum (kmax) and intensity of intrinsic ¯uorescence of Na+ /K+ -ATPase under increasing pressures are shown in Fig 2A The value for kmax was shifted from 341 to 345 nm with increasing pressure, ... forms Effects of pressure on ¯uorescence probes Fig E€ect of pressure on the intensity and kmax of intrinsic ¯uorescence of membrane-bound Na+ /K+ -ATPase (A) and solubilized Na+ / K+ -ATPase (B) Ten...

Ngày tải lên: 24/03/2014, 00:21

... Phosphoinositide 3-kinase: a key biochemical signal for cell migration in response to chemokines Immunol Rev 177, 217–235 Yasui, K & Komiyama, A (2001) Roles of phosphatidylinositol 3-kinase and ... Bourgoin, S., Jean, N., McColl, S.R & Naccache, P.H (1995) Diverging signal transduction pathways activated by interleukin-8 and related chemokines in human neutrophils: interleukin-8, but not NAP-2 ... phospholipase D in intact cells FEBS Lett 264, 87–90 Wakelam, M.J., Hodgkin, M & Martin, A (1995) The measurement of phospholipase D-linked signaling in cells, Methods Mol Biol 41, 271–278 Skepper, J.N...

Ngày tải lên: 07/03/2014, 15:20

... supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 ... prevention of aggregation of insulin B chain following reduction of disulphide bonds [13], refolding of guanidine hydrochloride (or urea)-denatured proteins [12,14] and prevention of inactivation of ... resealing solution Ó FEBS 2003 Measurement of a-Crystallin protects Na/ K- ATPase in red cell ghosts (Eur J Biochem 270) 2607 86 Rb flux while modifying Na/ K- ATPase The activity of Na/ K- ATPase...

Ngày tải lên: 17/03/2014, 03:20

... activation of p42/44 MAPK (also called ERK1/2) through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation of this ... such cells, using high ouabain concentrations, or palytoxin, which also alters [Na+ ]i/ [K+ ]i ratio by interaction with Na+ /K+ -ATPase, activation of a number of protein kinase signaling pathways ... other than inhibition or activation of Na+ /K+ -ATPase are known to affect intracellular signal pathways; e.g a rise in [Na+ ]i induced by gramicidin activates a stress-activated protein kinase [37]...

Ngày tải lên: 24/03/2014, 00:21

... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro ... C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and P.M Van Wyk 2002 Review of some recent developments in sustainable shrimp farming practices in Texas, Arizona and Florida J.Appl Aquac 12 ... regulation, In: Mantel L (Ed.) The biology of crustacean, Vol Internal anatomy and physiological regulation New York: Academic Press, pp:54-161 Menz A and B.F Blake 1980 Experiments on the growth of Penaeus...

Ngày tải lên: 02/04/2014, 10:20

... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro ... C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and P.M Van Wyk 2002 Review of some recent developments in sustainable shrimp farming practices in Texas, Arizona and Florida J.Appl Aquac 12 ... regulation, In: Mantel L (Ed.) The biology of crustacean, Vol Internal anatomy and physiological regulation New York: Academic Press, pp:54-161 Menz A and B.F Blake 1980 Experiments on the growth of Penaeus...

Ngày tải lên: 22/07/2014, 11:21

... staining profile by comparison to the rest of the neostriatum Like the patches, the staining of AChE was weaker in the MrD than in the rest of the neostriatum [9], and a layer of densely packed ... leu-enkephalin immunoreactive fibers and terminals was observed in the MrD in rat and cat [19] However, met-enkephalin immunostaining was reported to be more intensely packed in the rat MrD than in ... Histochemically distinct compartments in the striatum of human, monkey, and cat demonstrated by acetylthiocholinesterase staining Proceedings of the National Academy of Sciences of the United States of America...

Ngày tải lên: 10/08/2014, 05:21

... cell signalling properties of a fascinating molecule Planta 2005, 221:1-4 16 Sokolovski S, Hills A, Gay R, Garcia-Mata C, Lamattina L, Blatt MR: Protein phosphorylation is a prerequisite for intracellular ... measurement of a minimum of nine images corresponding to three independent experiments For quantification of the dark purple-coloured precipitate, an independent subtraction of the background was ... Nitric oxide is involved in growth regulation and re-orientation of pollen tubes Development 2004, 131:2707-2714 Cárdenas L, McKenna ST, Kunkel JG, Hepler PK: NAD(P)H Oscillates in Pollen Tubes...

Ngày tải lên: 12/08/2014, 03:21

... recommended dose of vitamin K is 10 mg [18] However, vitamin K therapy alone is inadequate during an emergency situation in which rapid cessation of bleeding or correction of INR is required [1,2,20] ... H, Haertel S, Knaub S, Kalina U, Jung K, Pabinger I: Pharmacokinetics of Beriplex P/N prothrombin complex concentrate in healthy volunteers J Thromb Haemost 2007, 98: 790-797 60 Kessler CM: Urgent ... 20-50 Not in label Not in label Not in label Not in label >20 7-31 15-45 C 5-25 Not in label Not in label Not in label Not in label Not in label 7-32 13-26 S Not in label Not in label Not in label...

Ngày tải lên: 13/08/2014, 11:23

... F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon Res 6, 349-360 Lin C.C., Draper P.N & De Braekeleer M ... obtain such acute regional assignment, combined with the use of high-resolution chromosome spreads, will be useful in several fields: localization of unique sequences, increasing the precision of ... number of grains pointed in this region is so high, that we could have reduced the number of spreads scored to one third and still maintain a significant signal In the first experiments, only 5% of...

Ngày tải lên: 14/08/2014, 19:23

... Review of Nutrition, 1990;10:23354 Minassian DC, Mehra V Blinded by cataract: Each year projection from the first epidemiological study of incidence of cataract blindness in India British Journal of ... groups were both examined by an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy and intraocular pressure ... alterations in serum cation concentration, can be known as a risk factor for cataract formation Na+ pump activity in lens is as in other cells and it is related to intracellular Na+ , extracellular K+ ...

Ngày tải lên: 03/11/2012, 09:49

... the regulation and localization of claudin expression It has been shown that protein kinases such as Protein Kinase A (PKA) and Protein Kinase C (PKC) can phosphorylate claudins (6,7) Phorbol ... 189-192 KTTS* [R /K] X[pS/pT] [R /K] XX[pS/pT] [R /K] [R /K] X[pS/ pT] KXX[pS/pT] 195-197 195-197 31-34 31-34 65-69 TPR TPR RIYS RIYS KVFDS [pS/pT]X[R /K] [pS/pT]X[R /K] RXXpS [R /K] XX[pS/pT] KXX[pS/pT] PKA PKC ... Ciccocioppo F., Ercolino E., Bascelli A., Cataldi A., and Miscia S Nuclear protein kinase C-delta: a possible check-point of cell cycle progression International journal of immunopathology and...

Ngày tải lên: 03/11/2012, 11:17

... the beginning of leaching, decreased after a peak, and then increased and decreased again This behavior indicates that the initial leaching is like Na+ leaching, and the surplus Ca2+ is drained ... between porous cups was determined as K1 -2, K2 -3, K3 -4 and the total hydraulic conductivity was determined as K1 -4 The changes in K1 -2, K2 -3, and K3 -4 were high during the initial stage, from to about ... Department of Chemical System Engineering, The University of Tokyo Sakai Y., Matsumoto, S and Sadakata M (2004) Alkali soil reclamation with flue gas desulfurization gypsum in China and assessment of...

Ngày tải lên: 05/09/2013, 09:38

... = dimQ H∗ (Bχ , Q) et 7.4.5 End of the proof of Proposition 7.1.7 We compare the K- groups via bcK bck sp K k C K η s k −− K( Bχ )Q K( Bχ )Q →s K( Bχ )Q K( Bχ )Q ∼ K( Bχ )Q ←− = ∼ = The first two isomorphisms ... K ¯ Gal (K/ K) K K 7.2.3 Proof of (8) Lemma 7.2.1 says that K( Bχ )Q = K( Bχ )Q so K it suffices to see that the Galois action on K( Bχ )Q is trivial However, 7.1.8 and ∼ = K ¯ the proof of 7.2.2 provide ... sheaf into irreducible perverse sheaves is independent of p, the calculation of the cohomology of Springer fibers (i.e., the stalks of the Springer sheaf), reduces to the calculation of stalks of intersection...

Ngày tải lên: 16/02/2014, 06:20

... Kwon OY, Lee JH, Kwon K, Min KJ, Jung SA, Kim AK, You KH, Tatar M & Yu K (2008) Drosophila short neuropeptide F signaling regulates growth by ERK-mediated insulin signaling Nat Cell Biol 10, ... Proc Natl Acad Sci USA 104, 7128–7133 Ament SA, Corona M, Pollock HS & Robinson GE (2008) Insulin signaling is involved in the regulation of worker division of labor in honey bee colonies Proc Natl ... 7, 6, and m in NaCl ⁄ Pi at °C, each step for h in 1 0K MWCO SnakeSkin Dialysis Tubing (Pierce, Rockford, IL, USA) The VgR recombinant antigen ( 30 kDa) was concentrated with a 10 kDa AmiconÒ...

Ngày tải lên: 18/02/2014, 08:20