na k atpase acts as signal transducer of endogenous cardiac glycosides
Báo cáo Y học: Na+/K+-ATPase as a signal transducer Zijian Xie and Amir Askari docx
... through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation of this cascade, unlike that of ROS generation, also ... Role of protein kinase C in the signal pathways that link Na+ / K+ -ATPase to ERK1/2 J Biol Chem 276, 42050–42056 32 Tian, J., Gong, X & Xie, Z (2001) Signal- transducing function of Na+ -K+ -ATPase ... L., Xie, Z., Huang, W.-H & Askari, A (1996) Partial inhibition of Na+ /K+ -ATPase by ouabain induces the Ó FEBS 2002 23 24 25 26 27 28 29 30 31 Na+ /K+ -ATPase as a signal transducer (Eur J Biochem...
- 6
- 427
- 1
Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx
... ouabain-insensitive isoform of Na+ /K+ -ATPase In most tissues, the Na+ /K+ -ATPase is a heterodimer consisting of a 112 kDa hydrolytic a-peptide, through which the vectorial transport of Na+ and K+ takes place, ... -insensitive a-isoforms of Na+ /K+ -ATPase in rats All isoforms of the catalytic a-peptide of Na+ /K+ -ATPase have similar, high affinity for ouabain [17] with Kd in the order of one or a few nanomolar [12,17] ... function of Na+ /K+ -ATPase J Biol Chem 275, 27832–27837 10 Liu, J., Tian, J., Haas, M., Shapiro, J.I., Askari, A & Xie, Z (2000) Ouabain interaction with cardiac Na+ /K+ -ATPase initiates signal cascades...
- 4
- 423
- 0
Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... mM NADH, lgámL)1 of pyruvate kinase, and 2.5 lgámL)1 LDH in a total volume of mL The reaction was initiated by an addition of 50 ng of Na+ /K+ -ATPase Na+ -dependent ATPase activity was also measured ... volume of mL The reaction was initiated by the addition of lg enzyme + Three activities of Na /K -ATPase were determined as follows Na+ /K+ -ATPase activity was measured by monitoring the decrease ... subjected to ultrasonication RESULTS Effects of pressure on Na+ /K+ -ATPase, Na+ -dependent ATPase and K+ -activated phosphatase activities It could be con®rmed that the Na+ /K+ -ATPase activity, as determined...
- 9
- 432
- 0
Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
... 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase in the range of the binding ... labeling leads to an inactivation of Na+ /K+ ATPase [51] pNPP docking experiments to the H4-H5 loop Table Eect of FITC-labeling on the phosphatase activities of Na+ / K+ ATPase and a H4-H5 loopGST ... Na+ /K+ ATPase [17] K+ activated phosphatase activity of the ATPase as well as the phosphatase activity of the H4-H5 loop protein were tested as described above [14,17] Labeling and purication of...
- 14
- 586
- 0
Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx
... of pNPPase activity of Na+ ⁄ K+ -ATPase Fig Activation of pNPPase by KCl in the presence of NaCl and oligomycin The pNPPase activity was assayed in a mixture containing lg Na+ ⁄ K+ -ATPase, the ... Stimulation of pNPPase activity of Na+ ⁄ K+ -ATPase H Homareda and M Ushimaru Table Phosphorylation of Na+ ⁄ K+ -ATPase from pNPP, Pi and ATP For phosphorylation by pNPP or Pi, Na+ ⁄ K+ -ATPase was incubated ... inactivated ATPase On the other hand, omission of NaCl completely inactivated ATPase (Fig 9B) 677 Stimulation of pNPPase activity of Na+ ⁄ K+ -ATPase Fig Na+ -dependent activation curves for Na+ ...
- 12
- 327
- 0
Na+, k+ ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity
... 1.1 Basic functions of the NKA isoforms in skeletal muscle NKA Function isoform α1 Key housekeeping isoform, large contribution to Na+ /K+ exchange mainly during basal conditions α2 Key α isoform ... Example of a three point calibration curve for the Na+ , K+ -ATPase β1 isoform 138 Figure 6.2 Representative blots of Na+ , K+ -ATPase at baseline, following ULLS and following four weeks of resistance ... phosphorylation via protein kinase A had no effect on the K+ affinity of α1/β1isoforms Activation by PKA phosphorylation alone of PLM influences the apparent Na+ affinity of the Na+ , K+ -ATPase α1/β1 and α2/β1...
- 188
- 370
- 0
Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
... Ó FEBS 2003 Measurement of a-Crystallin protects Na/ K- ATPase in red cell ghosts (Eur J Biochem 270) 2607 86 Rb flux while modifying Na/ K- ATPase The activity of Na/ K- ATPase was assessed by ouabainsensitive ... Na/ K- ATPase This type of assay provides additional evidence of the important role of the small heat shock proteins in cell protection Also, the protection from modification of ghost cell Na/ KATPase ... the supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose...
- 7
- 291
- 0
BÁO CÁO " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu s vannamei) " pptx
... so với thời điểm ngày, ngày ngày 3.3 Hoạt tính men Na/ K ATPase mang tôm thẻ chân trắng nghiệm thức có độ mặn thấp khác Hoạt tính men Na/ K ATPase mang tôm chuyển vào môi trường có độ mặn thấp trình ... tính men Na/ K ATPase mang tôm thẻ chân trắng theo thời gian độ mặn khác (chữ khác thể sai khác có ý nghĩa nghiệm thức (P
- 10
- 695
- 5
Báo cáo nghiên cứu khoa học: " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu svannamei)" ppsx
... so với thời điểm ngày, ngày ngày 3.3 Hoạt tính men Na/ K ATPase mang tôm thẻ chân trắng nghiệm thức có độ mặn thấp khác Hoạt tính men Na/ K ATPase mang tôm chuyển vào môi trường có độ mặn thấp trình ... tính men Na/ K ATPase mang tôm thẻ chân trắng theo thời gian độ mặn khác (chữ khác thể sai khác có ý nghĩa nghiệm thức (P
- 10
- 506
- 1
Báo cáo Y học: Endogenous cardiac glycosides, a new class of steroid hormones pot
... subunit of Na+ /K+ -ATPase [93,94] As one of the factors associated with blunted natriuresis, saltsensitive Dahl rats have a mutation in the a1 subunit of Na+ /K+ -ATPase [95] The mutated renal sodium ... some time that treatment of cells in culture with cardiac glycosides affects cell proliferation as well as the expression of isoforms of Na+ /K+ -ATPase When infused for weeks in rats, ouabain and ... M.N., Roukoyatkina, N.I., Ukhanova, M.A & Zhabko, E.P (1991) Endogenous plasma Na, K- ATPase inhibitory activity and digoxin-like immunoreactivity after acute myocardial infarction Cardiovasc Res...
- 9
- 651
- 0
Báo cáo khoa học: The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response pptx
... Oh SY, Woo SH, Kim KH, Kim JH, Kim HD & Kang HS (2001) Implication of small GTPase Rac1 in the activation of c-junN-terminal kinase and heat shock factor in response of heat shock J Biol Chem ... present case The heat-induced activation of kinases such as Akt has been shown to increase HSF1 activity Enhanced Ras maturation by heat stress was associated with a heightened activation of extracellular ... extracellular signal- regulated kinase (ERK), a key mediator of both mitogenic and stress signaling pathways, in response to subsequent growth factor stimulation [36] Given the importance of the plasma...
- 10
- 452
- 0
Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf
... heat shock proteins of murine cells Biochem Biophys Res Commun 204, 357–365 Yasuda K, Nakai A, Hatayama T & Nagata K (1995) Cloning and expression of murine high molecular mass heat shock proteins, ... activate JAKs directly, as JAKs localize mainly into the cytoplasm As Hsp105a, which localizes in the cytoplasm of cells, did not enhance the expression of Hsp70, Hsp105b may activate unknown factor(s) ... knockdown of STAT3 expression, 10 pmol of the STAT3 siRNA (Santa Cruz Biotechnology) or RISC-free control siRNA (Dharmacon, Chicago, IL, USA) was cotransfected into HEK293 cells with 1.5 lg of...
- 11
- 584
- 0
Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... and JAK tyrosine kinases participates in growth regulation of human breast carcinoma cells Oncogene 20, 2499–2513 Kanda N, Seno H, Konda Y, Marusawa H, Kanai M, Nakajima T, Kawashima T, Nanakin ... Proc Natl Acad Sci USA 97, 4227–4232 15 Konnikova L, Kotecki M, Kruger MM & Cochran BH (2003) Knockdown of STAT3 expression by RNAi induces apoptosis in astrocytoma cells BMC Cancer 3, 23 16 Nagel-Wolfrum ... Grammatikakis I, Rassidakis GZ, Zhang W, Fujio Y, Kunisada K et al (2005) Constitutive activation of JAK3 ⁄ STAT3 in colon carcinoma tumors and cell lines: inhibition of JAK3 ⁄ STAT3 signaling...
- 11
- 558
- 0
Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc
... Life Sciences, East Hills, NY, USA) and blocking with 10% skim milk (Becton-Dickinson, Franklin Lakes, NJ, USA) in NaCl ⁄ Pi containing 0.1% Tween 20 (Wako, Osaka, Japan) Signals were detected ... isothiocyanate (TRITC)-labeled swine anti-rabbit IgG (1 : 100; DAKO, Glostrup, Denmark) Structural analysis The putative signal sequence of mouse Skn and Gup1 was calculated using signalp 3.0 ... Kessler DP, Ekker SC, Young KE, Lee JJ, Moses K & Beachy PA (1995) The product of hedgehog autoproteolytic cleavage active in local and long-range signaling Nature 374, 363–366 Porter JA, Ekker...
- 14
- 499
- 0
Báo cáo khoa học: Action of palytoxin on apical H+/K+-ATPase in rat colon pdf
... measured Na+ - and K+ -stimulated ATPase activity found in the apical membranes of the colon is not due to Na+ / K+ -ATPase, but could be associated with some form(s) of the colonic H+ /K+ -ATPase, as ... subunit of Na+ /K+ ATPase in HEK293 cells [24,25] In Sf9 cells, however, expression of H+ /K+ -ATPase a subunit without a corresponding b subunit produces a ouabain-insensitive H+ /K+ ATPase [26] Finally, ... a subunit of colonic H+ /K+ -ATPase was expressed together in Xenopus oocytes with the b subunit of the H+/ K+ -ATPase from toad bladder or with either the b subunit of gastric H+ /K+ -ATPase or the...
- 7
- 351
- 0
Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot
... induce a conformational change of this receptor chain sufficient for the induction of a cytoplasmic signal In contrast, the first step of OSM signal transduction is binding of the cytokine to gp130 The ... unchanged Replacement of D6 of gp130 by the corresponding domain of the GCSFR leads to the abrogation of signal transduction of all tested cytokines As coprecipitation of this gp130 mutant and ... presented in this work (Fig 5) The formation of a signal transducing heterocomplex of gp130 and LIFR depends on the binding of a cytokine (e.g LIF) Site II of the ligand contacts the CBM of gp130 [14,15],...
- 11
- 583
- 0
Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A pot
... neuronal cell-specific suppressor cytokine signaling-3 (SOCS3) knockout mice exhibit higher leptin-induced phosphorylation of hypothalamic STAT3, loss of body weight, and suppression of food intake ... containing a Kozak sequence was cloned into pGEM-T easy The EcoRI–SmaI fragment of this plasmid was subcloned into the EcoRI–SmaI site of pEGFP-N1 (TaKaRa Bio, Otsu, Japan) For construction of a reporter ... the developing forebrain and spinal cord Mol Cell Neurosci 35, 272–282 Mori H, Hanada R, Hanada T, Aki D, Mashima R, Nishinakamura H, Torisu T, Chien KR, Yasukawa H & Yoshimura A (2004) Socs3...
- 12
- 484
- 0
Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... dominant negative variants of Stat5 Mol Cell Biol 16, 6141–6148 Matsumura I, Kitamura T, Wakao H, Tanaka H, Hashimoto K, Albanese C, Downward J, Pestell RG & Kanakura Y (1999) Transcriptional ... KE (1995) Direct stimulation of Jak ⁄ STAT pathway by the angiotensin II AT1 receptor Nature 375, 247–250 10 Guo Y, Mascareno E & Siddiqui MA (2004) Distinct components of Janus kinase ⁄ signal ... protein-dependent activation of angiotensinogen promoter: a cellular signal for hypertrophy in cardiac muscle Proc Natl Acad Sci USA 95, 5590–5594 Pan J, Fukuda K, Kodama H, Makino S, Takahashi T, Sano M,...
- 9
- 331
- 0
Báo cáo y học: "Attenuation of murine antigen-induced arthritis by treatment with a decoy oligodeoxynucleotide inhibiting signal transducer and activator of transcription-1 (STAT-1)" ppt
... Janus kinases and signal transducers and activators of transcription: their roles in cytokine signaling, development and immunoregulation Arthritis Res 2000, 2:16-32 Quandt K, Frech K, Karas H, ... Sugiura T, Tanaka M, Nakagawa M, Ichida H, Takagi K, Higami-Ohsako S, et al.: Amplification of the synovial inflammatory response through activation of mitogen-activated protein kinases and nuclear ... No Hückel et al IFN-γ as well as many other cytokines from Th cells regulate gene expression and cellular activation, proliferation and differentiation by means of the janus kinase /signal transducer...
- 13
- 449
- 0
Báo cáo khoa học: " A geminiviral amplicon (VA) derived from Tomato leaf curl virus (ToLCV) can replicate in a wide variety of plant species and also acts as a VIGS vector" pps
... reflects that the production of siRNA increased by more than two fold from 14 to 28 dpi in case of VIGS-PCNA(AC2N) The siRNA production in case of VIGS-PCNA(AC2M) was observed to be considerably ... period of time whereas the levels of PCNA-siRNA increased gradually Thus the silencing of the PCNA gene, induced by the VA vector, was evidently responsible for the stunting phenotype of the ... fractionated by the addition of 5% PEG, 0.5 M NaCl followed by centrifugation Small RNA was precipitated by adding volumes of ethanol to the supernatant as obtained above The enriched small RNA was...
- 13
- 428
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008