Immune memory and activation markers in systemic lupus erythematosus

... Layout…………………………………………………………….….….…….51 2.4.4 Staining Layout for Extracellular Staining………………………………….….….… 52 2.4.5 Staining Layout for Intracellular Staining………………………………… … …….53 2.4.6 Staining Procedures…………………………………………………………… ... patients is novel and original Besides investigating expression of Bcl2 and IL7R in T memory populations, expressions of CD25 and CXCR6 are also studie...

Ngày tải lên: 09/10/2015, 11:06

... expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon-induced transmembrane protein 1, ISG15, IRF7, ... repressive effect on the expressions of most IFI genes in PBMCs in vitro in this experiment The relative expressions of three of the representative genes (t...

Ngày tải lên: 09/08/2014, 01:22

... Hanly JG, Isenberg DA, Kalunian K, Nived O, et al.: The reliability of the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index in patients with systemic ... antibodies and nine (28%) had lupus anticoagulant TGF-β1 activation index: an in vitro measure of the in vivo efficiency of TGF-β1 activation To examine the ability of...

Ngày tải lên: 09/08/2014, 08:22

... expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon-induced transmembrane protein 1, ISG15, IRF7, ... repressive effect on the expressions of most IFI genes in PBMCs in vitro in this experiment The relative expressions of three of the representative genes (t...

Ngày tải lên: 09/08/2014, 13:22

... normal individuals However, VH5< /b> -51, one of < /b> only two functional % of < /b> functional independent V-genes DH gene < /b> 70 60 50 40 30 20 10 VH1< /b> VH2< /b> VH3< /b> VH4< /b> VH5< /b> VH6< /b> VH7< /b> D6 D7 VH < /b> gene < /b> family % of < /b> functional independent ... time point In an earlier investigation, Ravirajan and coworkers [7,8] demonstrated the pres...

Ngày tải lên: 09/08/2014, 01:21

... elsewhere in more detail [2,89] and their interplay is illustrated in Figure Proinflammatory high-density lipoproteins and nonrheumatic diseases Other diseases in which dysfunctional, presumably proinflammatory, ... PON1 influence levels of that enzyme and are also likely to alter HDL function; at least one also predisposes to SLE [65,66] Proinflammatory high-density lipo...

Ngày tải lên: 09/08/2014, 10:23

... the transcriptional levels of IFN-inducible chemokines in peripheral blood cells were closely associated with disease activity and organ damage in SLE, and may be useful in disease monitoring and ... McMahon M, Taylor M, Brahn E, Hahn BH, Tsao BP: Association of increased interferon-inducible gene expression with disease activity and lupus nephritis...

Ngày tải lên: 09/08/2014, 13:22

... an increased frequency of Th17 cells but not Th1 cells in peripheral blood, with an impaired balance of Th17 and Th1 responses We investigated whether the frequency of Th17 and Th1 cells in patients ... frequencies of Th17 and Th1 cells that was found in healthy controls was disrupted in lupus patients These observations indicate that the...

Ngày tải lên: 12/08/2014, 12:20

... In addition, TC measurements may be inherently less variable over time for physiological reasons and also because TC is measured in a laboratory by using standardized assays that have small interassay ... article as: Nikpour et al., Variability over time and correlates of cholesterol and blood pressure in systemic lupus erythematosus: a longitudinal...

Ngày tải lên: 12/08/2014, 14:22

... LDL, and by secreting IFN-γ, which then leads to increased in ammatory cytokine production This chronic production of in ammatory cytokines and proteases may lead to thinning of the plaque wall and ... Page of 10 Other cytokines T cells The in ammatory cytokine TNF-α appears to play an important role in the initiation and perpetuation of atherosclerotic lesions in...

Ngày tải lên: 12/08/2014, 15:22

... Prevalence and risk factors of carotid plaque in women with systemic lupus erythematosus Arthritis Rheum 1999, 42:51-60 Salmon JE, Roman MJ: Accelerated atherosclerosis in systemic lupus erythematosus: ... Controlling cardiovascular risk factors in systemic lupus erythematosus J Musculoskel Med 1998, 15: 42-52 El-Magadmi M, Ahmad Y, Wajed J, Bruce IN: The bimodal...

Ngày tải lên: 09/08/2014, 01:22

... immunofluorescent ANA pictures, and also helped edit the manuscript MS designed and coordinated the study, performed the immunoassays and the data analysis, and also edited the manuscript All authors read and ... coexist and thus about 80% of SSc has one of these autoantibodies [30] In PM/DM, patients with antiaminoacyl tRNA synthetase antibodies have antibodies again...

Ngày tải lên: 09/08/2014, 08:22

... Overproduction of type I interferons in SLE matures dendritic cells (DCs) into immunogenic antigen-presenting cells that strongly contribute to the T- cell and B-cell hyperactivity characteristic of this ... with disease activity, and the suppressive function of these cells was intact in cultures without antigen-presenting cells The functional activity of both patient and h...

Ngày tải lên: 09/08/2014, 13:22

... are the C-type lectin receptors (CTLRs) The CTLRs comprise a family of evolutionarily conserved proteins containing one or more C-type lectin domains, and may bind carbohydrate moieties in a calcium-dependent ... proinflammatory cytokines, and increased ability to promote increased proliferation and activation of allogeneic control T cells [21] In the present study,...

Ngày tải lên: 09/08/2014, 13:22

... with lupus disease activity indices or other clinical features was determined High IFN scores were positively associated with increased disease activity as indicated by the SLEDAI and the Systemic ... B-lymphocyte activating factor in systemic lupus erythematosus and rheumatoid arthritis in relation to autoantibody levels, disease measures and time Lupus 2006, 15:570-576...

Ngày tải lên: 09/08/2014, 14:22