... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... article as: Chu et al.: Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response Radiation ... dilution of 1:100 in 1% BSA The secondary antibody solution was then aspirated, and the cells were washed twice in PBS The cells were then incubated in the dark with PI (1 μg/ml) in PBS for 30 min,...