dna damage and telomeric loss in yeast aging

... and has been involved in type of packaging likewise in the submission process PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval ... were held hour at 4°C and incubated at 37°C for 24 hours The study of initial DNA damage was completed in the University of Granada (Spain) Initial radiation-induced DNA damage in PBLs was measured ... association between initial DNA damage, measured as DSB, and RIA Ionizing radiation (IR) kills cells by damaging the structure and function of genomic DNA The response of cells to this damage and their...

Ngày tải lên: 09/08/2014, 09:20

... late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new ... the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval of the version ... damage and RIA in LABC patients [23] Taking into account the above background and our previously observations, we explored the clinical association between initial DNA damage and RIA in relation...

Ngày tải lên: 09/08/2014, 09:20

... examination Ovaries and uteri were removed from the mice, fixed in 10% phosphate-buffered formalin (pH 7.4) for 24 h, dehydrated, and embedded in paraffin Sections were cut 3-lm thick and stained ... Molecular Cloning A Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory press, Cold Spring Harbor, NY 25 Hogan, B., Beddington, R., Costantini, F & Lacy, E (1994) Tail bleeding In Manipulating the ... was to examine the in vivo estrogenic effects of BPA on the female reproductive tract and bone by using ArKO mice MATERIALS AND METHODS Materials A standard rodent chow (NMF) was obtained from...

Ngày tải lên: 24/03/2014, 03:21

... contamination of reagents or equipment with amplicon DNA Uracil -DNA glycosylase (UNG) is a DNA repair enzyme that will cleave uracil-containing DNA while leaving the natural, thymine-containing DNA ... containing UNG and incubated at 30°C for 20 slightly greater than the standards that did not contain UNG and were not incubated prior to RT-PCR The yintercept value for the reactions not containing ... contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime RT-PCR provides a rapid method to assess and optimize...

Ngày tải lên: 19/06/2014, 08:20

... contamination of reagents or equipment with amplicon DNA Uracil -DNA glycosylase (UNG) is a DNA repair enzyme that will cleave uracil-containing DNA while leaving the natural, thymine-containing DNA ... containing UNG and incubated at 30°C for 20 slightly greater than the standards that did not contain UNG and were not incubated prior to RT-PCR The yintercept value for the reactions not containing ... contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime RT-PCR provides a rapid method to assess and optimize...

Ngày tải lên: 20/06/2014, 04:20

... al., 2005a) and its differential binding to DNA is a determining factor in its involvement in DNA replication, exhibiting distinct origin DNA binding properties from its association with DNA ends ... eukaryotic single-stranded (ss) DNA binding protein and it is required for DNA replication, recombination and repair RPA helps recruit DNA primase/polymerase α to the origins, stabilizing ssDNA in the ... both Dna2 and Fen1 in vitro in an ATPindependent manner (Kang et al., 2009) Stimulation depends on the specific protein-protein interaction between the N-terminal domain of Dna2 and Mph1 Since...

Ngày tải lên: 28/06/2014, 05:20

... types The interaction data types include both direct physical interactions - protein complexes and proteinDNA interactions - and indirect functional interactions genetic interactions and interactions ... motifs containing a transcription factor that regulates two physically interacting proteins can be combined into a single theme corresponding to a protein complex whose component proteins are controlled ... [23] seek to interpret genetic interactions in yeast through the physical interaction network between this organism’s genes that can be generated from protein-protein and protein -DNA interaction...

Ngày tải lên: 06/08/2014, 18:21

... the potential damage caused by fire in any forest stand in Catalonia, depending on the site and the structure of the stand In addition to predicting the degree of damage at the stand level, the ... thinning and prescribed burning on crown fire severity in ponderosa pine forests, Int J Wildl Fire 11 (2002) 1–10 [32] Rigolot E., Predicting postfire mortality of Pinus halepensis Mill and Pinus ... to in rather uneven stands and approaches to in homogeneous stands All the variables included in the stand damage model were significant at the 0.05 level (Tab III) The coefficient of determination...

Ngày tải lên: 07/08/2014, 16:21

... iodide and mithramycin stainings Results are listed in table II In F sylvatica, the GC content was 40.0 ± 0.2 %; in the tortuosa variety, 39.8 ± 0.2 % These values are not significantly different and ... error in cytophotometric determination of nuclear DNA content in plants, Plant Syst Evol 158 (1988) 87-96 advice [17] Laurie D.A., Bennett M.D., Nuclear DNA content in the genera Zea and Sorghum Intergeneric, ... is extremely rapid and convenient: it allows determinations of nuclear DNA content [13] and of AT/GC base composition in a genome [15] Favre and Brown [ 12] developed a fast and simple flow cytometry...

Ngày tải lên: 08/08/2014, 14:21

... (2C 2.85 pg; 41 % GC) was selected as an internal standard The Q petraea and Q robur clones originated from donor trees in northeastern France (Lorraine) The Q pubescens donor tree was from the ... taking emissions of > 590 or 408-530 nm Each event was analysed for pulse versus integral to avoid doublets, as explained in a general review of the method by Marie and Brown (1993) Nuclear DNA ... base-specific dye and applying the fifth root relationship of Godelle et al (1993): RESULTS AND DISCUSSION Results are given in tableI They show a relatively uniform nuclear DNA content and base composition...

Ngày tải lên: 08/08/2014, 18:21

... exclusively involved in acute inflammatory responses and act as the first line of defence against invading pathogens needs modification The neutrophil presence in a number of chronic inflammatory ... transient increase in RANKL levels in the joint, supplementing that normally provided by the chronic inflammatory cells in synovial tissues This may result in rapid stimulation of bone erosion during ... are pivotal in causing tissue destruction, either directly or indirectly We are still to determine the importance of the neutrophil in regulating the bone destruction that occurs in RA and other...

Ngày tải lên: 09/08/2014, 10:20

... phenotyping, intracellular and extracellular cytokine profiling in ageing mice 6.4.1 T cell surface marker phenotyping 6.4.2 Cytokine profiling assay: Intracellular and extracellular cytokines 6.4.3 ... plasma levels and reduced age-dependent accumulation of 1) 8OHdG in liver and heart; 2) 8-Iso-PGF2 in heart and urine and 3) PCC in liver and kidney However, a 12-month RSV intake resulted in significant ... using F344 rats for validating methods in ageing biomarker assays and to establish oxidative damage levels in ageing rodents 28 Table 1.3 Phase and studies using F2 hybrid mice for the middle and...

Ngày tải lên: 12/09/2015, 08:20

... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage ... damage checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage checkpoints In S.cerevisiae, ... and effectors In budding yeast, Mec1, (a phosphatidylinositol kinase like kinase, PIKK and an ortholog of human ATR) is responsible for detection of genomic insults and initiation of signalling,...

Ngày tải lên: 14/09/2015, 14:03

... protective effects against hypoxia and reoxygenation This is mainly ascribed to its ability to maintain redox status [17], intervening in the DNA repair system [18–20] and restoring antioxidant capacities ... detection of single-strand and double-strand breaks Pyruvate reduces DNA damage during ⁄ after hypoxia Cells cultivated in Petri dishes (25 mm in diameter) were suspended in 0.5 mL of NaCl ⁄ Pi, and cell ... reoxygenation but is ineffective at maintaining glutathione levels during hypoxia Glutathione is one of the main antioxidant compounds in the cell, and it is also essential for DNA synthesis and repair...

Ngày tải lên: 18/02/2014, 16:20

... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage ... DNA making it ideal for labeling fragments in apoptotic cells The enzyme will also label single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in ... used methods for detecting DNA damage in situ is TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining (1) TUNEL staining was initially described as a method for staining cells that have undergone...

Ngày tải lên: 11/04/2014, 09:47

... cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10] We determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum -DNA damage ... were the kind gift of Dr Eddie Reed Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, 100 μg/mL penicillin/streptomycin, mM L-glutamine and 0.2 units/mL insulin Cells ... cisplatin efficacy because of its abilities to impair NER by inhibiting induction of p53 and XPC and to activate MMR by maintaining high levels of MSH2 and enhancing platinum accumulation in tumors...

Ngày tải lên: 20/06/2014, 07:20

... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... then washed in PBS twice, and the anti-gH2AX antibody was added at a dilution of 1:500 in 1% BSA and incubated overnight at 4℃ Again, the cells were washed twice in PBS before incubating in the dark ... produce DNA interstrand and/ or intrastrand cross-links [29,30] As has been known, bifunctional alkylating agents induce collapsed replication forks that can lead to either cell cycle arrest, DNA...

Ngày tải lên: 09/08/2014, 09:20

... more efficiently and in a coordinated manner Further investigations will define the precise mechanisms of the striking coordination between transcription, chromatin and splicing, and the functional ... II pausing within introns Materials and methods Yeast strains and experimental conditions Wild-type fission yeast cells (972 h-) were grown in rich yeast extract media at 32°C before being harvested ... accumulates at the 3’ end of introns, most notably in lowly expressed genes Intriguing studies in budding yeast have recently reported splicing-related pausing of Pol II during transcription [40-42]...

Ngày tải lên: 09/08/2014, 23:20

... certain types of damage induced in DNA, principally interstrand crosslinks [20] In contrast, Cluster includes key genes whose proteins mediate cell cycle arrest in response to genotoxic and other cellular ... Tax-binding proteins generated from our physical mapping efforts, DNAPK was in the top five best represented binding proteins and occupied a top tier ranking via our functional clustering for DNA ... 278(39):37736 Defining first neighbor interactions of the known Taxbinding proteins In this section we conducted a query for immediate binding partners of a selected group of known Tax-binding proteins Our...

Ngày tải lên: 13/08/2014, 05:21

... shuttling in response to DNA damage may be a consequence of their inability to be ubiquitinated Lysine residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in ... demonstrate that DNA damage results in increased mono-ubiquitination of Tax Mutation of Tax amino acids known to be targets of ubiquitination (K280 and K284) inhibited DNA damage- induced ubiquitination ... cytoplasm following DNA damage To examine this possibility, the distribution of wild-type Tax and a Tax mutant containing arginine substitutions in all lysine residues (TaxK1-10R) was examined by immunofluorescent...

Ngày tải lên: 13/08/2014, 06:20