oxidative stress dna damage and apoptosis in male infertility
Effect of coenzyme q10 on oxidative stress, glycemic control and inflammation in diabetic neuropathy a double blind randomized clinical trial pdf
... improvement in FBS or glycated hemoglobin, but in the control group, the insulin sensitivity decreased and the fasting insulin and insulin resistance increased, which shows a protective effect in our intervention ... Instrument Ltd, United Kingdom HOMA Calculator ver 2.2 (University of Oxford) by analyzing the two parameters fasting glucose and fasting insulin: insulin sensitivity (%S) and HOMA (insulin resistance), ... reduce insulin resistance, oxidative stress, and in ammation and also increase insulin sensitivity Thus, future studies should emphasize longer periods of supplementation and larger doses in milder...
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Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
... late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new ... fractionation) and in the methodology used (PFGE, comet assay, gamma-H2AX induction) could help to explain the discrepancies observed Initial DNA damage levels in breast cancer patients Radiation-induced apoptosis ... the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval of the version...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... zvd-fmk By inhibiting apoptosis, we were able to reduce spontaneous DNA breakage and estimate rejoining as the initial DNA released minus residual DNA released without the interference of apoptosis ... radiation induced DNA breakage, which is attributable to rejoining, continued diminishing (Figure 5) The blockade of apoptosis thus allowed us to efficiently determine rejoining without the inference ... by apoptosis (which can be inhibited by caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity...
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Báo cáo y học: "Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats" potx
... hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation Clin Sci (Lond) 2008, 115:91-97 31 Maines MD, Mayer RD, Ewing JF, McCoubrey WK Jr: Induction of ... decrease in blotting for I-CAM in aorta (Figure 4c) but not in heart (P < 0.05) in comparison to the HS-saline group (Figure 4f) NaHS reduces I/R-induced oxidative stress Compared to the HS-saline ... enzymes, HO-1 and HO-2 Indeed, Maines et al [30] reported increased levels of HO-1 in I/R injuries; moreover, HO-1 was found to improve resistance to oxidative stress [32] and modulate inflammatory...
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Báo cáo y học: "he Association Among Lipoprotein-associated Phospholipase A2 Levels, Total Antioxidant Capacity and Arousal in Male Patients with OSA"
... a minimum of 10 sec, was considered as hypopnea Individuals with an apnea hypopnea index (AHI) >5 were diagnosed as OSAS and included in the study Determining of Arousals An arousal was defined ... triglyceride levels were increased in patients above median arousal and TAC was reduced in these subjects Other demographic and laboratory findings were comparable in two groups According to the arousal ... arousal index was associated with increased LP-PLA2 level The elevated levels of circulating LP-PLA2 may explain prone to sudden death due stroke and AMI in patients with OSA and increased arousal index...
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Tài liệu OXIDATIVE STRESS – MOLECULAR MECHANISMS AND BIOLOGICAL EFFECTS doc
... enormous interest and intensive research in this field that prompted us to develop this book idea In addition to interest in basic science, there is also a growing interest in medicine, agriculture and ... samples and involvement of oxidative stress in diseases and aging is covered by S Argỹelles and colleagues The complicated way of our understanding of hydrogen roles in biological systems from inert ... with Intuitively, it is accepted that oxidative stress is the situation when oxidative damage is increased that, in turn, can be explained as an imbalance between ROS production and elimination in...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... Over-expression of HSP70 inhibits oxidative stress- induced apoptosis in C2C12 myogenic cells We next examined the effects of HSP70 over-expression on oxidative stress- induced apoptosis in C2C12 myogenic ... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore, ... no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding domain, was essential for this specific function of the protein Recent...
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báo cáo hóa học: " Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord" potx
... outside of demyelinated lesions Our finding of changes in cytokine expression following NG2 ablation may also be important in understanding changes in demyelination and remyelination in the NG2 null ... signaling by b1 integrins [50] and promotion of signaling by receptors for the growth factors PDGF and FGF [27,51] In vitro studies also indicate that NG2-dependent signaling by b1 integrins and ... free-floating in 0.1 M PBS containing 0.02% sodium azide For immunostaining, free-floating sections were first incubated for 60 at room temperature in 0.1 M PBS containing 5% normal goat serum and...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
... al., 2005a) and its differential binding to DNA is a determining factor in its involvement in DNA replication, exhibiting distinct origin DNA binding properties from its association with DNA ends ... eukaryotic single-stranded (ss) DNA binding protein and it is required for DNA replication, recombination and repair RPA helps recruit DNA primase/polymerase α to the origins, stabilizing ssDNA in the ... both Dna2 and Fen1 in vitro in an ATPindependent manner (Kang et al., 2009) Stimulation depends on the specific protein-protein interaction between the N-terminal domain of Dna2 and Mph1 Since...
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OXIDATIVE STRESS – ENVIRONMENTAL INDUCTION AND DIETARY ANTIOXIDANTS doc
... external and internal origin Therefore, this book mainly contains the information on oxidative stress induced by physical and chemical factors and a portion of the book includes the information ... and replacing other metal ions in their binding centers (Valko et al., 2007) The detailed description of toxicokinetics of lead and cadmium, induction and role of oxidative stress in neurochemical ... 2011b) In this definition, the dynamic character of ROS-involving processes and their effects on core and regulatory processes in living organisms are underlined To date, development of oxidative stress...
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OXIDATIVE STRESS – MOLECULAR MECHANISMS AND BIOLOGICAL EFFECTS doc
... enormous interest and intensive research in this field that prompted us to develop this book idea In addition to interest in basic science, there is also a growing interest in medicine, agriculture and ... samples and involvement of oxidative stress in diseases and aging is covered by S Argỹelles and colleagues The complicated way of our understanding of hydrogen roles in biological systems from inert ... with Intuitively, it is accepted that oxidative stress is the situation when oxidative damage is increased that, in turn, can be explained as an imbalance between ROS production and elimination in...
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Báo cáo y học: " Resveratrol retards progression of diabetic nephropathy through modulations of oxidative stress, proinflammatory cytokines, and AMP-activated protein kinase" potx
... plasma glucose and creatinine concentrations, and increased plasma insulin level, to moderate extents in the STZ-diabetic rats Additionally, RSV remarkably alleviated oxidative stress and prevented ... accumulating evidence indicating that renal inflammation played a key role in the pathogenesis of DN it was demonstrated that diabetes increased proinflammatory cytokines including TNF-a, IL-1b and ... attenuated in the diabetes-affected renal tissues (Figure 1C, D) RSV ameliorated oxidative stress in the STZ-induced type diabetic kidneys The indicators of oxidative stress including the contents...
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Báo cáo y học: "24-hour changes in circulating prolactin, follicle-stimulating hormone, luteinizing hormone and testosterone in male rats subjected to social isolation" doc
... apparently attempting to escape (sniffing and chewing at the bars and suddenly dashing around their cage) while those housed in groups spent more time sleeping and feeding [13] This indicates that ... of plasma prolactin, luteinizing hormone (LH), folliclestimulating hormone (FSH) and testosterone levels at different time points within a 24-h cycle in growing male rats kept in isolation or ... housing of animals is associated with an increase in aggression of males [10,19], hyperresponsiveness to several stressors [12] and an increase in time spent attempting to escape and decrease in...
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báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway" pptx
... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... has been reported in a few papers Both in vitro and in vivo studies showed that Osthole possessed an anticancer effect by inhibiting human cancer cells growth and inducing apoptosis [13-17] It ... checkpoint is controlled by Cdc2 and Cyclin B1 [27], and some anticancer-drugs could induce G2/M arrest through down-regulating the expressions of Cyclin B1 and Cdc2 [28] The results in our study...
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báo cáo khoa học: "Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251" pdf
... receptor-associated tyrosine kinases or growth factor receptor intrinsic tyrosine kinases, besides antagonizing the function of relevant kinases or receptors, targeting the over-expressed ligands that inappropriately ... glioma in vitro and in vivo bFGF exerts functions via its specific binding to the high affinity transmembrane tyrosine kinase receptors [23] and the low affinity FGF receptors (FGFR1-4) [24] The binding ... Muller-Newen G, Schaper F: Principles of interleukin (IL)-6-type cytokine signaling and its regulation Biochem J 2003, 374:1-20 13 Haque SJ, Sharma P: Interleukins and STAT Signaling Vitam Horm 2006,...
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Báo cáo y học: " tế ngành y học dành cho các bạn tham khảo đề tài: Early Oxidative stress increases Fas ligand expression in endothelial cells" ppt
... preliminary experiments showing that H2O2-induced FasL expression in endothelial cells is enhanced by vanadate, a protein tyrosine kinase inhibitor, but reduced by genistein, a tyrosine kinase inhibitor ... HUVECs and stained with diaminobenzidine and Giemsa solutions Arrows point to apoptotic neutrophils with condensed and/ or fragmented nuclei and cytoplasm positively stained with diaminobenzidine ... paraformaldehyde for 15 After rinsing with PBS, plates were blocked with blocking buffer (PBS containing 3% bovine serum albumin and 2% goat serum: Sigma-Aldrich) and incubated for 60 minutes at room temperature...
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Báo cáo khoa học: "Cysteine protease activation and apoptosis in Murine norovirus infection" docx
... remained viable and morphologically indistinct from control cells Blebbing, swelling, and cytoplasmic leaking were evident in cells infected in the presence of the caspase inhibitor (Figure 4) In ... apoptosis [12] The mechanisms for inhibiting apoptosis target a handful of proteins, including caspases, Bcl-2, TNF (tumor necrosis factor), NFκB, PKR (dsRNA-dependent protein kinase), p53, and ... better understanding of the different processes that were occurring in the caspase inhibited and non-inhibited infected RAW264.7 cells, two additional stains were used Hoechst staining is commonly...
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Báo cáo y học: "Endothelin-1 enhances fibrogenic gene expression, but does not promote DNA synthesis or apoptosis in hepatic stellate cell" doc
... or Fas-ligand [19] were used to induce apoptosis in HSC In serum deprivation apoptosis, control was made with 10% serum In Fas-ligand induced apoptosis, control was run without Fas-ligand There ... FCS containing μg/ml of Fas enhancer (mouse IgG) and 10 to 50 μg/ml Fas-ligand for 24 h To investigate the influence of ET-1 on Fas-ligand induced apoptosis, HSC were cultured with increasing concentrations ... fibrogenesis and in particular in HSC matrix production and apoptosis remains controversial Therefore we examined cell proliferation, apoptosis and extracellular matrix metabolism of ET-treated HSC in...
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Molceular genetics of y chromosome in male infertility
... chromodomain implicated in chromatin binding, and a catalytic domain involved in acylation reactions (Lahn and Page, 1997; 1999b) The predicted coding regions of CDY1 and CDY2 were 98% identical in amino ... binding to the androgen binding region (ABD) encoded by exon 4-8 Specific point mutatons associated with male infertility were found in the TAD and ABD regions, respectively The DNA binding region ... Genes involved in the regulation of male reproduction in the mouse Source: Matzuk and Lambs (2002) Figure Genetic etiologies of human male infertility Developmental disorders causing human male infertility...
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Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf
... radical formation in diabetic rats induced by streptozotocin Life Sci 56, 1789–1798 Hodges NJ, Green RM, Chipman JK & Graham M (2007) Induction of DNA strand breaks and oxidative stress in HeLa cells ... longitudinal sections are shown in (A) (magnification ·20; scale bars = mm) The hemotoxylin and eosin-stained sections of the left ventricle are shown in (B) and Masson’s trichrome stained sections ... mitochondria damage) (Figs and and Table 1) A number of studies have demonstrated that CYP2E1 is a loosely coupled enzyme, and can generate ROS and promote oxidative stress in cells during its catalytic...
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