oxidative stress dna damage and apoptosis in male infertility

... improvement in FBS or glycated hemoglobin, but in the control group, the insulin sensitivity decreased and the fasting insulin and insulin resistance increased, which shows a protective effect in our intervention ... Instrument Ltd, United Kingdom HOMA Calculator ver 2.2 (University of Oxford) by analyzing the two parameters fasting glucose and fasting insulin: insulin sensitivity (%S) and HOMA (insulin resistance), ... reduce insulin resistance, oxidative stress, and in ammation and also increase insulin sensitivity Thus, future studies should emphasize longer periods of supplementation and larger doses in milder...

Ngày tải lên: 22/07/2016, 08:43

... late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new ... fractionation) and in the methodology used (PFGE, comet assay, gamma-H2AX induction) could help to explain the discrepancies observed Initial DNA damage levels in breast cancer patients Radiation-induced apoptosis ... the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval of the version...

Ngày tải lên: 09/08/2014, 09:20

... zvd-fmk By inhibiting apoptosis, we were able to reduce spontaneous DNA breakage and estimate rejoining as the initial DNA released minus residual DNA released without the interference of apoptosis ... radiation induced DNA breakage, which is attributable to rejoining, continued diminishing (Figure 5) The blockade of apoptosis thus allowed us to efficiently determine rejoining without the inference ... by apoptosis (which can be inhibited by caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity...

Ngày tải lên: 09/08/2014, 09:20

... hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation Clin Sci (Lond) 2008, 115:91-97 31 Maines MD, Mayer RD, Ewing JF, McCoubrey WK Jr: Induction of ... decrease in blotting for I-CAM in aorta (Figure 4c) but not in heart (P < 0.05) in comparison to the HS-saline group (Figure 4f) NaHS reduces I/R-induced oxidative stress Compared to the HS-saline ... enzymes, HO-1 and HO-2 Indeed, Maines et al [30] reported increased levels of HO-1 in I/R injuries; moreover, HO-1 was found to improve resistance to oxidative stress [32] and modulate inflammatory...

Ngày tải lên: 13/08/2014, 21:21

... a minimum of 10 sec, was considered as hypopnea Individuals with an apnea hypopnea index (AHI) >5 were diagnosed as OSAS and included in the study Determining of Arousals An arousal was defined ... triglyceride levels were increased in patients above median arousal and TAC was reduced in these subjects Other demographic and laboratory findings were comparable in two groups According to the arousal ... arousal index was associated with increased LP-PLA2 level The elevated levels of circulating LP-PLA2 may explain prone to sudden death due stroke and AMI in patients with OSA and increased arousal index...

Ngày tải lên: 25/10/2012, 11:00

... enormous interest and intensive research in this field that prompted us to develop this book idea In addition to interest in basic science, there is also a growing interest in medicine, agriculture and ... samples and involvement of oxidative stress in diseases and aging is covered by S Argỹelles and colleagues The complicated way of our understanding of hydrogen roles in biological systems from inert ... with Intuitively, it is accepted that oxidative stress is the situation when oxidative damage is increased that, in turn, can be explained as an imbalance between ROS production and elimination in...

Ngày tải lên: 12/02/2014, 16:20

... Over-expression of HSP70 inhibits oxidative stress- induced apoptosis in C2C12 myogenic cells We next examined the effects of HSP70 over-expression on oxidative stress- induced apoptosis in C2C12 myogenic ... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore, ... no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding domain, was essential for this specific function of the protein Recent...

Ngày tải lên: 16/03/2014, 01:20

... outside of demyelinated lesions Our finding of changes in cytokine expression following NG2 ablation may also be important in understanding changes in demyelination and remyelination in the NG2 null ... signaling by b1 integrins [50] and promotion of signaling by receptors for the growth factors PDGF and FGF [27,51] In vitro studies also indicate that NG2-dependent signaling by b1 integrins and ... free-floating in 0.1 M PBS containing 0.02% sodium azide For immunostaining, free-floating sections were first incubated for 60 at room temperature in 0.1 M PBS containing 5% normal goat serum and...

Ngày tải lên: 19/06/2014, 22:20

... al., 2005a) and its differential binding to DNA is a determining factor in its involvement in DNA replication, exhibiting distinct origin DNA binding properties from its association with DNA ends ... eukaryotic single-stranded (ss) DNA binding protein and it is required for DNA replication, recombination and repair RPA helps recruit DNA primase/polymerase α to the origins, stabilizing ssDNA in the ... both Dna2 and Fen1 in vitro in an ATPindependent manner (Kang et al., 2009) Stimulation depends on the specific protein-protein interaction between the N-terminal domain of Dna2 and Mph1 Since...

Ngày tải lên: 28/06/2014, 05:20

... external and internal origin Therefore, this book mainly contains the information on oxidative stress induced by physical and chemical factors and a portion of the book includes the information ... and replacing other metal ions in their binding centers (Valko et al., 2007) The detailed description of toxicokinetics of lead and cadmium, induction and role of oxidative stress in neurochemical ... 2011b) In this definition, the dynamic character of ROS-involving processes and their effects on core and regulatory processes in living organisms are underlined To date, development of oxidative stress...

Ngày tải lên: 28/06/2014, 11:20

... enormous interest and intensive research in this field that prompted us to develop this book idea In addition to interest in basic science, there is also a growing interest in medicine, agriculture and ... samples and involvement of oxidative stress in diseases and aging is covered by S Argỹelles and colleagues The complicated way of our understanding of hydrogen roles in biological systems from inert ... with Intuitively, it is accepted that oxidative stress is the situation when oxidative damage is increased that, in turn, can be explained as an imbalance between ROS production and elimination in...

Ngày tải lên: 28/06/2014, 11:20

... plasma glucose and creatinine concentrations, and increased plasma insulin level, to moderate extents in the STZ-diabetic rats Additionally, RSV remarkably alleviated oxidative stress and prevented ... accumulating evidence indicating that renal inflammation played a key role in the pathogenesis of DN it was demonstrated that diabetes increased proinflammatory cytokines including TNF-a, IL-1b and ... attenuated in the diabetes-affected renal tissues (Figure 1C, D) RSV ameliorated oxidative stress in the STZ-induced type diabetic kidneys The indicators of oxidative stress including the contents...

Ngày tải lên: 10/08/2014, 05:21

... apparently attempting to escape (sniffing and chewing at the bars and suddenly dashing around their cage) while those housed in groups spent more time sleeping and feeding [13] This indicates that ... of plasma prolactin, luteinizing hormone (LH), folliclestimulating hormone (FSH) and testosterone levels at different time points within a 24-h cycle in growing male rats kept in isolation or ... housing of animals is associated with an increase in aggression of males [10,19], hyperresponsiveness to several stressors [12] and an increase in time spent attempting to escape and decrease in...

Ngày tải lên: 10/08/2014, 09:20

... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... has been reported in a few papers Both in vitro and in vivo studies showed that Osthole possessed an anticancer effect by inhibiting human cancer cells growth and inducing apoptosis [13-17] It ... checkpoint is controlled by Cdc2 and Cyclin B1 [27], and some anticancer-drugs could induce G2/M arrest through down-regulating the expressions of Cyclin B1 and Cdc2 [28] The results in our study...

Ngày tải lên: 10/08/2014, 10:21

... receptor-associated tyrosine kinases or growth factor receptor intrinsic tyrosine kinases, besides antagonizing the function of relevant kinases or receptors, targeting the over-expressed ligands that inappropriately ... glioma in vitro and in vivo bFGF exerts functions via its specific binding to the high affinity transmembrane tyrosine kinase receptors [23] and the low affinity FGF receptors (FGFR1-4) [24] The binding ... Muller-Newen G, Schaper F: Principles of interleukin (IL)-6-type cytokine signaling and its regulation Biochem J 2003, 374:1-20 13 Haque SJ, Sharma P: Interleukins and STAT Signaling Vitam Horm 2006,...

Ngày tải lên: 10/08/2014, 10:21

... preliminary experiments showing that H2O2-induced FasL expression in endothelial cells is enhanced by vanadate, a protein tyrosine kinase inhibitor, but reduced by genistein, a tyrosine kinase inhibitor ... HUVECs and stained with diaminobenzidine and Giemsa solutions Arrows point to apoptotic neutrophils with condensed and/ or fragmented nuclei and cytoplasm positively stained with diaminobenzidine ... paraformaldehyde for 15 After rinsing with PBS, plates were blocked with blocking buffer (PBS containing 3% bovine serum albumin and 2% goat serum: Sigma-Aldrich) and incubated for 60 minutes at room temperature...

Ngày tải lên: 11/08/2014, 08:21

... remained viable and morphologically indistinct from control cells Blebbing, swelling, and cytoplasmic leaking were evident in cells infected in the presence of the caspase inhibitor (Figure 4) In ... apoptosis [12] The mechanisms for inhibiting apoptosis target a handful of proteins, including caspases, Bcl-2, TNF (tumor necrosis factor), NFκB, PKR (dsRNA-dependent protein kinase), p53, and ... better understanding of the different processes that were occurring in the caspase inhibited and non-inhibited infected RAW264.7 cells, two additional stains were used Hoechst staining is commonly...

Ngày tải lên: 12/08/2014, 04:20

... or Fas-ligand [19] were used to induce apoptosis in HSC In serum deprivation apoptosis, control was made with 10% serum In Fas-ligand induced apoptosis, control was run without Fas-ligand There ... FCS containing μg/ml of Fas enhancer (mouse IgG) and 10 to 50 μg/ml Fas-ligand for 24 h To investigate the influence of ET-1 on Fas-ligand induced apoptosis, HSC were cultured with increasing concentrations ... fibrogenesis and in particular in HSC matrix production and apoptosis remains controversial Therefore we examined cell proliferation, apoptosis and extracellular matrix metabolism of ET-treated HSC in...

Ngày tải lên: 13/08/2014, 13:20

... chromodomain implicated in chromatin binding, and a catalytic domain involved in acylation reactions (Lahn and Page, 1997; 1999b) The predicted coding regions of CDY1 and CDY2 were 98% identical in amino ... binding to the androgen binding region (ABD) encoded by exon 4-8 Specific point mutatons associated with male infertility were found in the TAD and ABD regions, respectively The DNA binding region ... Genes involved in the regulation of male reproduction in the mouse Source: Matzuk and Lambs (2002) Figure Genetic etiologies of human male infertility Developmental disorders causing human male infertility...

Ngày tải lên: 16/09/2015, 15:53

... radical formation in diabetic rats induced by streptozotocin Life Sci 56, 1789–1798 Hodges NJ, Green RM, Chipman JK & Graham M (2007) Induction of DNA strand breaks and oxidative stress in HeLa cells ... longitudinal sections are shown in (A) (magnification ·20; scale bars = mm) The hemotoxylin and eosin-stained sections of the left ventricle are shown in (B) and Masson’s trichrome stained sections ... mitochondria damage) (Figs and and Table 1) A number of studies have demonstrated that CYP2E1 is a loosely coupled enzyme, and can generate ROS and promote oxidative stress in cells during its catalytic...

Ngày tải lên: 22/03/2014, 16:20