Analysis of type IX collagen in zebrafish axial development

... Amino acid sequence alignment of Zebrafish, Human, Mouse and Chick ColIXα2 88 Phylogenetic tree of FACIT family comprising of chains of collagen IX, collagen XII, collagen XIV and collagen XIX ... chains of zebrafish type IX collagen and type II collagen alpha in the tail bud during segmentation and post-segmentation period 106 Translation inhibition of col...

Ngày tải lên: 15/09/2015, 22:06

... real-time quantification RT-PCR detection of type X collagen mRNA Gene Primer Sequence Type X collagen Forward Reverse Forward Reverse 5'-AGTGCTGTCATTGATCTCATGGA-3' 5'-TCAGAGGAATAGAGACCATTGGATT-3' ... cartilage Since type X collagen is a marker of hypertrophic cartilage and osteoarthritic cartilage, our data suggest that mechanical strain above certain threshold (2.5%)...

Ngày tải lên: 20/06/2014, 00:20

... was that in RA the use in an ELISA of fragments of the intact CII molecule, particularly CB10, as antigen markedly increased the sensitivity of detection of antibodies in serum against NCII from ... Purification of CB peptides of type II collagen (CII) (a) Carboxymethylcellulose chromatogram and corresponding Coomassie blue protein stain (CII) illustrating th...

Ngày tải lên: 09/08/2014, 01:23

... blockade of macropinocytosis prevented intracellular accumulation of CII and resulted in profound blockade of antigen presentation to T cells The involvement of macropinocytosis in uptake of autoantigens, ... down-regulation of antigen presentation and inhibits activation of CII-specific T cells in in vitro antigen presentation assays Since T cells sp...

Ngày tải lên: 09/08/2014, 08:22

... RESULTS Expression analysis of zfgfap-l Temporal expression analysis of zfgfap-l Expression analysis of zfgfap-l by whole-mount in situ hybridization Expression analysis of zfgfap-l in mindbomb ... analysis of zfgfap-l in wild type embryos Expression analysis of zfgfap-l in mib-/- embryos Expression of GFAP in wild type and zfgfap-l morpholino...

Ngày tải lên: 05/10/2015, 22:31

... the hindbrain and its deficiency in morphants is in line with expression of these genes in this part of brain ctrl/brain A gli2b MO/brain B ctrl/trunk D gli2 MO/brain C gli2b MO/trunk E gli2 ... that in Gli2b morphant (data now shown) These results indicated a minor role of both gli2 and gli2b in regulating expression of nkx2.2 in the ventral hindbrain Due to d...

Ngày tải lên: 15/09/2015, 17:10

... containing gli2 genes 101 Results 3 .2 Expression analysis of gli2b in zebrafish 3 .2. 1 Temporal expression of gli2b in zebrafish embryos Temporal expression of gli2b was first compared with that of ... hpf gli2b dorsal smu-/-/48 hpf B gli2b dorsal syu-/-/48 hpf C gli2b dorsal cyclopamine/48 hpf E gli2b WT/48 hpf D gli2b dorsal shh mRNA/48 hpf F Fig 3-10 gli2b expression...

Ngày tải lên: 15/09/2015, 17:10

... affected in mib-/- embryos 11 1 Fig 3 -12 gli2b and Fgf3/8 11 3 Fig 3 -13 Validation of gli2b MO for blocking translation of zebrafish 11 6 gli2b Fig 3 -14 gli2b MO blocks zebrafish gli2b mRNA splicing 11 7 ... Concluding remarks 13 1 13 4 13 6 13 6 13 9 14 3 14 6 14 8 15 7 15 9 16 1 16 3 16 5 16 6 16 7 17 0 Chapter V Conclusion 17 3 Chapter VI References 17 5...

Ngày tải lên: 15/09/2015, 17:10

... attack (15) The present clinical trial evaluated the safety and efficacy of UC -II in the treatment of the knee in OA patients Materials and Methods Study Design This clinical trial (Human Clinical ... Compliance was assessed by capsule count at visits 3, 4, and and review of subject diary Efficacy and Safety Variables Efficacy and safety measu...

Ngày tải lên: 26/10/2012, 09:48

... function that are involved in the induction of peripheral tolerance in an orally tolerized mouse model of arthritis R218 Our findings indicate that the serum levels of proinflammatory and anti-inflammatory ... production of IL-10, TGF-β and IFN-γ by lymphocytes of orally tolerized mice R216 To examine the effect of oral CII administration on the activity of immun...

Ngày tải lên: 09/08/2014, 01:23

... edema and deformity of the hindpaws; however, SIA is primarily an enthesopathy characterized by the proliferation of fibroblasts and the formation of periarticular enthesophytes of cartilage and ... severity of arthritis Conclusion To understand the importance of CIX for cartilage integrity and stability and in the disease process of arthritis, we used a t...

Ngày tải lên: 09/08/2014, 08:22

... determined by intracellular (IC) staining of lines PMA (phorbol 12-myristate 13-acetate)/ionomycin activated T cells (a) We detected interferon (IFN)-γ staining but no interleukin (IL)-4 staining ... of a more generalised immune reactivity to extracellular matrix proteins in SSc Laminin and, less often, type IV collagen can induce proliferation of SSc lymphocytes [16] Autoanti...

Ngày tải lên: 09/08/2014, 08:22

... containing substitutions within the immunodominant determinant of type II collagen that effectively suppress autoimmune arthritis in DR4 transgenic mice Both of these analog peptides contain substitutions ... a decrease in the incidence and severity of arthritis, the production of antibody against CII was also significantly decreased (Table and Figure 3) Discussion Bi...

Ngày tải lên: 09/08/2014, 08:22

... human clinical trials In a randomized, double-blind, multicenter, and controlled phase II clinical trial involving 236 patients with severe active RA, a decrease in the number of swollen joints ... and safety of CCII in RA patients further, we treated two groups of RA patients with oral CCII or MTX in a randomized, doubleblind, multicenter, and controlled...

Ngày tải lên: 12/08/2014, 11:22

... shown) In order to obtain the mathematical relation of enhancing parameters of 3-chlorophenol on OH radical generation during ozonation, the initial velocities in the mixture of various amounts of ... evaluate OH radical generation in water containing humic acid during ozonation 20-60 µM of aqueous ozone was rapidly mixed with DMPO solution containing humic...

Ngày tải lên: 05/09/2013, 08:40