Characterization of gli2 2b genes in zebrafish hindbrain development 1

... affected in mib-/- embryos 11 1 Fig 3 -12 gli2b and Fgf3/8 11 3 Fig 3 -13 Validation of gli2b MO for blocking translation of zebrafish 11 6 gli2b Fig 3 -14 gli2b MO blocks zebrafish gli2b mRNA splicing 11 7 ... Concluding remarks 13 1 13 4 13 6 13 6 13 9 14 3 14 6 14 8 15 7 15 9 16 1 16 3 16 5 16 6 16 7 17 0 Chapter V Conclusion 17 3 Chapter VI References 17 5...

Ngày tải lên: 15/09/2015, 17:10

... the hindbrain and its deficiency in morphants is in line with expression of these genes in this part of brain ctrl/brain A gli2b MO/brain B ctrl/trunk D gli2 MO/brain C gli2b MO/trunk E gli2 ... that in Gli2b morphant (data now shown) These results indicated a minor role of both gli2 and gli2b in regulating expression of nkx2.2 in the ventral hindbrain Due to d...

Ngày tải lên: 15/09/2015, 17:10

... containing gli2 genes 101 Results 3 .2 Expression analysis of gli2b in zebrafish 3 .2. 1 Temporal expression of gli2b in zebrafish embryos Temporal expression of gli2b was first compared with that of ... hpf gli2b dorsal smu-/-/48 hpf B gli2b dorsal syu-/-/48 hpf C gli2b dorsal cyclopamine/48 hpf E gli2b WT/48 hpf D gli2b dorsal shh mRNA/48 hpf F Fig 3-10 gli2b expression...

Ngày tải lên: 15/09/2015, 17:10

... (b) AG nb035-sv1 2389 801 AATTTTGGATACTAACATGCTACAAAATAGGAAGGAGATTCATTTACTAA N F G Y nb035-sv2 1570 521 GAGATTCCATTTGATCCAAGGGAAGCATATGGAAGGAGATTCATTTACTAA E I P F D P R E A Y G R R F I Y 901 ... 301 AG domain-structure nb035-sv1 / Cl1Contig431 * AG * GT gene-model AAAAGATCAGTTCATAAAAGATCTGTTGAAGGAAGGAGATTCATTTACTAA K R S V H K R S V E G R R F I Y Figure organization and alternative...

Ngày tải lên: 14/08/2014, 21:20

... Amino acid sequence alignment of Zebrafish, Human, Mouse and Chick ColIXα2 88 Phylogenetic tree of FACIT family comprising of chains of collagen IX, collagen XII, collagen XIV and collagen XIX ... chains of zebrafish type IX collagen and type II collagen alpha in the tail bud during segmentation and post-segmentation period 106 Translation inhibition of col...

Ngày tải lên: 15/09/2015, 22:06

... 18 4 3 2 10 1 1 1 1 2 2 1 22 12 17 10 Subgenus Betulenta: B lenta 10 6 3 4 1 - - 1 14 11 10 3 10 2 10 3 10 6 - - 4 4 - 2 3 5 - 3 2 - 1 1 2 2 - 1 1 - - - - 20 16 20 17 - 17 11 12 11 13 Subgenus Betula: ... 17 % - Isoform Gene 01A 01 1A Ia: n.q IIIa: 51 IVa: 10 0 Va: 46 VIIa: 75 01A06 1A Ia: n.q IIIa: 51 IVa: 10 0 Vb: 23 VIIa: 75 01B 01 1B Ib: 69 IIIb: 1...

Ngày tải lên: 12/08/2014, 03:20

... for their role in maintaining HIV1 latency The only purpose of our microarray analysis was to identify candidate genes potentially involved in the control of the HIV latency For this reason, we ... latency In this study, the role of the HDAC inhibitor NaB on HIV-1 latently infected cells gene expression was explored using microarrays Since chromatin remodeling...

Ngày tải lên: 13/08/2014, 09:21

... Mopholino targeting at the splice junction of exon and intron of irf6 pre-mRNA (E3I3) in zebrafish that strongly suggests a critical role of Irf6 in proper gastrulation and early embryogenesis In ... profiling microarray analysis of the E3I3 injected embryos Perturbation of Irf6 either by injection of Irf6 mRNA encoding only its DNA-binding domain (Sabel et al., 20...

Ngày tải lên: 01/10/2015, 17:26

... Mopholino targeting at the splice junction of exon and intron of irf6 pre-mRNA (E3I3) in zebrafish that strongly suggests a critical role of Irf6 in proper gastrulation and early embryogenesis In ... profiling microarray analysis of the E3I3 injected embryos Perturbation of Irf6 either by injection of Irf6 mRNA encoding only its DNA-binding domain (Sabel et al., 20...

Ngày tải lên: 09/10/2015, 10:49

... and the other was exposed to anoxic conditions (use of nitrogen gas and addition of 20 mg-N/L NaNO3 to the cup) The PUR was estimated from the slope of the line describing the linear decrease in ... facility where the retention time of wastewater in the sewer line is lower than those in the UCT processes In sequencing batch reactors (SBRs), the PURs are hig...

Ngày tải lên: 05/09/2013, 09:38

... mgÆmL)1 BSA and stored at )20 °C Electrogenic BSP uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL ... addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as well as of K+ (6.25 ± 0.16 unitsÆmEq)1 K+, with lg valinomycin) and valinomycin (0.5...

Ngày tải lên: 20/02/2014, 01:20

... survey of missing enzymes in the KEGG PATHWAY database suggests that the lysine-degradation pathway map for P aeruginosa is missing 28 of its 62 enzymes (45%) Lysine catabolism is notable for ... that there are multiple known enzyme genes around a target pathway hole as A ¼ {a1 ,a2 , ,a| A|}, where |A| is the number of known enzyme genes that are adjace...

Ngày tải lên: 07/03/2014, 09:20

... in the higher activity observed in Vasconcellea spp.: (1) a higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these ... relationships of cysteine proteinases it can be deduced that the common ancestor of the genera Carica and Vasconcellea already contained at least two d...

Ngày tải lên: 07/03/2014, 11:20

... Variations in the residues involved in the binding at site of the human FABP1, and possibly in zebrafish FABP1a and zebrafish FABP1b, may reflect differences in the binding affinities of the zebrafish ... during embryogenesis and therefore may not perform a function equivalent to mammalian FABP1 during zebrafish embryogenesis The distinct patterns of ex...

Ngày tải lên: 07/03/2014, 12:20

... Characterization of flavin in hexose oxidase T Rand et al apparent extinction coefficient of the flavin in the mutant enzyme (e437 ¼ 5.5 mm)1Æcm)1), only half of the flavin is incorporated in the mutant ... to the in uence of the remaining free amino acids in the hydrolysate HOX, GOOX comparison According to the model of FAD in HOX presented...

Ngày tải lên: 16/03/2014, 14:20