identification of functional candidate genes for drought tolerance in rice

... the final manuscript. Acknowledgements We thank Robert Haynes for the countless hours spent in caring for and maintaining precise records of these RI lines and Sung Kim for his help in analyzing ... crosso- vers in Aec2 of chromosome 1, consisting of 33 lines with a single crossover in Aec2 and 16 lines with a crossover in both Aec1 and Aec2. In addition, 2 lines were established using mice ... num- bers of recombinant events, there do not appear to be any crossover hotspots. Disease profiling of the recombinant inbred lines SjS-like disease in our NOD-derived mouse lines, including C57BL/6.NOD-Aec1Aec2,...

Ngày tải lên: 09/08/2014, 13:22

... as of today, especially for a large number of DNA samples. The newly identified SNP were used for exclusion of the four investigated candidate genes in canine eye disease for a large number of ... results in four canine retina-specific genes by SSCP analysis and DNA sequencing in 29 breeds of dogs. In addition, we use iden- tified single nucleotide polymorphisms (SNP) for indirect exclusion of ... 5 (page number not for citation purposes) In this study we investigated four retina-specific genes, ATP binding cassette transporter retina (ABCA4), con- nexin 36 (CX36), c-mer tyrosin kinase receptor...

Ngày tải lên: 11/08/2014, 08:20

... expression in its flanking region. These findings clarify the crucial role of RIN in the transcriptional regulation of ripening initiation and progression. Background Ripening processes of many kinds of ... ces in the LeEXP1 and LeACS4 promotersaswellasanalyzingthepossible CArG boxes. Binding of RIN to the CArG boxes in the ripening-induced gene promoters in vivo and in vitro To distinguish the genes ... encoding a protein similar to thioredoxin y1. An increase in the transcript level of this gene was observed with ripening in normal fruit but not in the rin mutant, suggesting that RIN possibly induces...

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 11/08/2014, 11:21

... set in green house in Faculty of Agronomy, Hanoi University for Agriculture, Vietnam during spring cropping season in 2006. 2.2. Planting Seed of rice cultivars were incubated and sown in ... stomatal conductance contributed to drought tolerance in rice plant. The larger number of root per plant was the reason for better recovering of rice plant after drought treatment. REFERENCES ... parameters: At three stages as before draining, during drought treatment and 4 days after recovering, 4 plants of each rice cultivars were randomly selected for measuring photosynthetic Pham Van...

Ngày tải lên: 06/08/2014, 18:21

Ngày tải lên: 12/08/2014, 03:20

Ngày tải lên: 12/08/2014, 15:20

... the discriminatory peaks found in this study (Continued) Coronin, actin binding protein, 1B 55.7 Keratin 9 62 Tubulin, beta polypeptide 47.7 Tubulin, alpha, ubiquitous 50.1 Beta-actin 41.7 Actin, gamma ... E: Diagnostic markers for diseases: SELDI-TOF profiling of pig sera for PRRS. Dev Biol 2008, 132:399–403. 21. Zhong L, Taylor DL, Whittington RJ: Proteomic profiling of ovine serum by SELDI-TOF MS: optimisation, ... pig proteins identified in other studies and the discriminatory peaks found in this study (Continued) Chaperonin containing TCP1, subunit 5 (epsilon) protein 59.6 Chaperonin containing TCP1,...

Ngày tải lên: 05/03/2014, 17:20

... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... reliable reference gene in HEP2 cells infected with the same strain of HSV-1). These findings therefore highlight the impor- tance of re-evaluating the choice housekeeping genes when making even slight ... microtiter plates in a final volume of 25 μl under the following cycle conditions: 50°C for 2 minutes, 95°C for 2 minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds. Data...

Ngày tải lên: 18/06/2014, 18:20

... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... reliable reference gene in HEP2 cells infected with the same strain of HSV-1). These findings therefore highlight the impor- tance of re-evaluating the choice housekeeping genes when making even slight ... microtiter plates in a final volume of 25 μl under the following cycle conditions: 50°C for 2 minutes, 95°C for 2 minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds. Data...

Ngày tải lên: 20/06/2014, 01:20

... experimental transmission of infection and disease in inoculees, (ii) examination of seroprevalence rates in flocks with high and low PDD incidences, (iii) documentation of serocon- version accompanying development of ... since the first description of PDD. Although a viral etiology has long been suspected, a convincing lead for a responsible viral pathogen has been lacking. By combining veterinary clinical investigation with ... kidneys, brain) were placed into RNAlater within 1 minute of death and frozen within 2 minutes of death. Duplicate samples were collected for histopatho- logic diagnosis of PDD. Inclusion criteria PDD-positive...

Ngày tải lên: 20/06/2014, 01:20

... a final concentration of 0.3% (v/v) and incubated at 37 o C for 24 h with shaking. Sterility testing was performed by placing 0.5 ml of the killed vaccine into 25 ml of enrichment broth, incubated ... Results of mannose-resistant haemagglutination: The two ETEC strains were examined for mannose-resistant haemagglutinating activity using Sheep Red Blood Cells. Both strains were tested using overnight ... results of haemagglutination of cultures of each strain are presented in Table 1. Mannose-resistant haemagglutination was observed at 37 o C, but not at 18 o C for both strains, confirming the...

Ngày tải lên: 22/06/2014, 12:20

... signaling [18,19]. PAK proteins constitute a family of serine/threonine kinases that utilizes the CRIB (Cdc42/Rac interactive binding) domain to bind to small GTPases; members of the family include ... TRAC-1, for T-cell RING protein in activa- tion. As shown in Figure 4g, TRAC-1 has a RING finger domain, which is characteristically found in a class of pro- teins collectively called ubiquitin ligases ... protein-tyrosine kinases for ubiquitination and therefore to down-regulate their signaling [30]. The TRAC-1 hit we obtained has a trunca- tion in the carboxyl terminus but still retains the intact...

Ngày tải lên: 06/08/2014, 18:20

... list of thousands of genes, and it is not clear which genes are important for what. A key idea, originating from engineering science in general and computer science in particular, is the notion of ... (GATA3) regulating one of this subset, the ITK gene, a promoter analysis was performed. The final module of 37 disease-associated genes consisted of genes listed in public databases as having relevant ... ITK. Finally, using available databases, 47 genes were identified as interacting with GATA3. The expression data were used to filter out 10 inactive genes, thus leaving a final module of 37 disease-associated...

Ngày tải lên: 06/08/2014, 19:20

... School of Medicine, Department of Medicine, IB424G, 975 West Walnut Street, Indianapolis, IN 46202, USA. 2 Indiana University School of Medicine, Department of Psychiatry, PR116, Indianapolis, IN ... the introgressed interval. The goal of this study was to identify candidate genes that may influence alcohol consumption by comparing gene expression in five brain regions of alcoh ol-naïve inbred ... onsidered necessary for an ani- mal model of alcoholism [39,40]. Subsequently, inbred alcohol-preferring (iP) and -nonpreferring (iNP) strains were established; these inbred strains maintain highly divergent...

Ngày tải lên: 09/08/2014, 20:21

... information in expres- sion profiles [2]; applied linear transformations to mea- sure the discriminative information of genes [3]; and combined information from multiple assessments [4]. One of ... mutual influence of neighboring genes within a pathway, including the effects of genetic buffering. Because the TIF is c omputed for one pathway at a time, PWEA cannot detect genetic buffering exerted ... experimental findings. As would be expected of any method designed to aid in the interpre- tation of expression data, the pathways reported in PWEA give insights into the nature of the different types of...

Ngày tải lên: 09/08/2014, 20:21

... straightforward method for the identification of host genes involved in viral infection. We succeeded in establishing a cDNA-AFLP system to help track changes in gene expression patterns in N. ... these genes influence the accumulation of BaMV protein. Nine of the 15 genes showed either a positive or a negative influence on the accumulation of BaMV protein. Six knockdown plants showed an increase ... accumulation of BaMV in plants. An interesting observation was that eight of the nine plants showing an increase in BaMV coat protein were associated with cell rescue, defense, death, aging, signal...

Ngày tải lên: 11/08/2014, 11:21

... protein, a glycine rich protein and a pectinesterase inhibitor-domain containing pro- tein), and one gene encoding a flavin-bin ding monooxy- genase-likefamilyproteinwhichhastheinferred function in ... genes detected in this study are, though incomplete, important in determining rhizome initiation and development in O. longistami- nata. Detailed examination of the functions of this set of genes ... in RT include those encoding two CEN-like proteins, two meiosis 5 proteins, two GA response proteins, and two auxin-responsive proteins. Also, the expression levels of two meiosis 5 protein genes...

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 11/08/2014, 12:21